Hypoxia-Induced TGFBI as a Serum Biomarker for Laboratory Diagnosis and Prognosis in Patients with Pancreatic Ductal Adenocarcinoma.

Abstract:

OBJECTIVE:To explore novel biomarkers for patients with pancreatic ductal adenocarcinoma (PDAC), from the perspective of tumor hypoxia. METHODS:We screened 29 differentially expressed and hypoxia-upregulated genes from the Oncomine database. A total of 12 secretory proteins that interact with hypoxia-inducible factor 1 (HIF-1A) were selected by STRING (protein-protein interaction networks). After excluding enzymes and collagens, insulin-like growth factor-binding protein 3 (IGFBP3), glycoprotein NBM (GPNMB), transforming growth factor-β-induced (TGFBI), and biglycan (BGN) were detected by sandwich enzyme-linked immunosorbent assay (ELISA) in patients with cancer and healthy control individuals. RESULTS:The serum level of TGFBI was significantly elevated in patients with PDAC, compared with healthy controls; the assay could discriminate among cases of PDAC in different clinical stages. The amount of TGFBI was significantly decreased after treatment. The combination of TGFBI and cancer antigen (CA) 19-9 was more accurate than TGFBI or CA 19-9 alone as diagnostic markers. Also, TGFBI might be used as a prognostic marker according to the PROGgeneV2 Pan Cancer Prognostics Database. CONCLUSIONS:Serum TGFBI, combined with CA 19-9, offers higher diagnostic value than other methods for patients with PDAC. Also, TGFBI might be used as a prognostic marker.

journal_name

Lab Med

journal_title

Laboratory medicine

authors

Yang L,Cui R,Li Y,Liang K,Ni M,Gu Y

doi

10.1093/labmed/lmz063

subject

Has Abstract

pub_date

2020-07-08 00:00:00

pages

352-361

issue

4

eissn

0007-5027

issn

1943-7730

pii

5598921

journal_volume

51

pub_type

杂志文章
  • The Use and Misuse of Statistical Methods in Cytopathology Studies: Review of 6 Journals.

    abstract:Background:The misuse of statistical methods in diagnostic accuracy studies has been criticized in many publications. Objective:To assess the use and misuse of statistical methods in medical journal articles. Methods:We reviewed 141 original articles from 6 cytopathology journals published in 2014. Results:In total,...

    journal_title:Laboratory medicine

    pub_type: 杂志文章,评审

    doi:10.1093/labmed/lmy036

    authors: Bahar B,Pambuccian SE,Barkan GA,Akdas Y

    更新日期:2019-01-01 00:00:00

  • Naegleria fowleri That Induces Primary Amoebic Meningoencephalitis: Rapid Diagnosis and Rare Case of Survival in a 12-Year-Old Caucasian Girl.

    abstract::Primary amoebic meningoencephalitis (PAM) is a rare and almost always fatal disease that is caused by Naegleria fowleri, a freshwater thermophilic amoeba. Our case involves an adolescent female who presented with fever of unknown origin. A lumbar puncture was performed, and the Wright-Giemsa and Gram stained cerebrosp...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmw008

    authors: Dunn AL,Reed T,Stewart C,Levy RA

    更新日期:2016-05-01 00:00:00

  • Antinuclear Antibodies: Marker of Diagnosis and Evolution in Autoimmune Diseases.

    abstract::Antinuclear antibodies (ANAs) are autoantibodies that attack self-proteins within cell nucleus structures; their presence in serum may indicate an autoimmune disease. Also, positive ANA test results have been obtained in chronic infectious diseases, cancers, medication-related adverse events, and even healthy individu...

    journal_title:Laboratory medicine

    pub_type: 杂志文章,评审

    doi:10.1093/labmed/lmy024

    authors: Sur LM,Floca E,Sur DG,Colceriu MC,Samasca G,Sur G

    更新日期:2018-07-05 00:00:00

  • Impact of Time Lapse on ASCP Board of Certification Medical Laboratory Scientist (MLS) and Medical Laboratory Technician (MLT) Examination Scores.

    abstract:BACKGROUND:Research in several professional fields has demonstrated that delays (time lapse) in taking certification examinations may result in poorer performance by examinees. Thirteen states and/or territories require licensure for laboratory personnel. A core component of licensure is passing a certification exam. A...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1309/LMNM534LIACPBZWH

    authors: ASCP Board of Certification Research and Development Committee.,Brown KA,Fenn JP,Freeman VS,Fisher PB,Genzen JR,Goodyear N,Houston ML,O'Brien ME,Tanabe PA

    更新日期:2015-07-01 00:00:00

  • Serum Levels of IL-6, IL-8, TNF-α, and TGF-β in Chronic HBV-Infected Patients: Effect of Depression and Anxiety.

    abstract:Objective:To assess the effects of depression and anxiety on serum cytokine levels in patients with chronic hepatitis B (CHB) infection. Methods:In this cross-sectional study, 60 healthy control individuals and 60 patients with CHB participated after filling out standard questionnaires. We examined their serum interle...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmx064

    authors: Bahramabadi R,Fathollahi MS,Hashemi SM,Arababadi AS,Arababadi MS,Yousefi-Daredor H,Bidaki R,Khaleghinia M,Bakhshi MH,Yousefpoor Y,Torbaghan YE,Arababadi MK

    更新日期:2017-12-22 00:00:00

  • Assessment of the Reliability of the Sysmex XE-5000 Analyzer to Detect Platelet Clumps.

    abstract:OBJECTIVE:Platelet counts generated by automated analyzers on blood specimens that contain platelet clumps are often inaccurate and require verification by blood-smear review. In this study, we assessed the reliability of the Sysmex XE-5000 instrument to detect platelet clumps. METHOD:We reviewed automated complete bl...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmw016

    authors: Hawkins J,Gulati G,Uppal G,Gong J

    更新日期:2016-08-01 00:00:00

  • Oxidized low-density lipoprotein and upregulated expression of osteonectin and bone sialoprotein in vascular smooth muscle cells.

    abstract:BACKGROUND:Oxidative stress has been associated with the progression of atherosclerosis and activation of genes that lead to increased deposition of proteins in the extracellular matrix. Bone sialoprotein (BSP) and osteonectin are proteins involved in the initiation and progression of vascular calcification. OBJECTIVE...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1309/LMUJWVQFW6CJMSOQ

    authors: Farrokhi E,Samani KG,Chaleshtori MH

    更新日期:2014-10-01 00:00:00

  • Developmental Defects Associated With DNA Copy Number Gain of Chromosome 2q33.1: A Case Report and Review of Literature.

    abstract::Caspases play a vital role during apoptosis. In addition to apoptosis, caspases play a role in cytokine gene induction and work to inhibit apoptosis. In order for individuals to thrive with useful tissue growth, the rate of cell growth and division must surpass the rate of cell division. It is well established that ex...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmx086

    authors: Gupta A,Yo J,Huang G,Soong L,Dong J

    更新日期:2018-03-21 00:00:00

  • Current Practice and Regional Variability in Recommendations for Patient Preparation for Laboratory Testing in Primary Care.

    abstract:BACKGROUND:Preparation of the patient for laboratory tests is crucial. Our aim was to investigate the current practice and regional variability of recommendations regarding patient preparation for laboratory testing. METHODS:A call for data was posted by email. Spanish laboratories were invited to fill out and submit ...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmz092

    authors: Salinas M,López-Garrigós M,Flores E,Leiva-Salinas C

    更新日期:2020-05-06 00:00:00

  • Verification of 20 Mathematical Formulas for Discriminating Between Iron Deficiency Anemia and Thalassemia Trait in Microcytic Anemia.

    abstract:BACKGROUND:Currently, more than 45 mathematical formulas based on simple red blood cell (RBC) parameters have been proposed for differentiating between iron deficiency and thalassemia in microcytic anemia, of which 20 are relatively new and have not been thoroughly independently verified. The study goal was to verify t...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmaa030

    authors: Hoffmann JJML,Urrechaga E

    更新日期:2020-11-02 00:00:00

  • Iron Overload in an HFE Heterozygous Carrier: A Case Report and Literature Review.

    abstract::Hereditary hemochromatosis (HH) is an autosomal recessive disorder of iron metabolism characterized by increased iron absorption and tissue deposition. Three loss-of-function mutations in the hemochromatosis gene (HFE), namely, C282Y (c.845G>A), H63D (c.187C>G), and S65C (c.193A>T), account for the vast majority of HH...

    journal_title:Laboratory medicine

    pub_type: 杂志文章,评审

    doi:10.1093/labmed/lmy065

    authors: Turbiville D,Du X,Yo J,Jana BR,Dong J

    更新日期:2019-04-08 00:00:00

  • Performance Characteristics and Clinical Value of the Lipoprotein-Associated Phospholipase A2 by an Enzymatic Kinetic Method.

    abstract:OBJECTIVE:To analyze the performance characteristics, stability, and clinical value of lipoprotein-associated phospholipase A2 (Lp-PLA2) using an enzymatic kinetic method. METHODS:The performance characteristics included reference intervals, precision, and accuracy. We assessed Lp-PLA2 stability by comparing Lp-PLA2 c...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmy086

    authors: Lu M,Lv L

    更新日期:2019-07-16 00:00:00

  • Cording in Disseminated Mycobacterium chelonae Infection in an Immunocompromised Patient.

    abstract::Cording is a phenomenon in which acid fast bacilli grow in parallel and was previously used as a means of presumptive microscopic identification of Mycobacterium tuberculosis (TB). However, this process has been shown in multiple other nontuberculous mycobacterial (NTM) species. Here we present the case of an immunoco...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmaa082

    authors: Olson G,McNulty MC,Mullane K,Beavis KG,Tesic V

    更新日期:2020-09-21 00:00:00

  • Establishing the Cause of Anemia in a Premature Newborn Infant.

    abstract::The three major causes of anemia in neonates are blood loss, decreased red blood cell production, and increased degradation of erythrocytes. Establishing the cause of anemia in a neonate born prematurely can be challenging. Clinically, fetomaternal hemorrhage (FMH) can be difficult to diagnose-the condition often pres...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmy026

    authors: den Besten G,van der Weide K,Schuerman FABA,Michael Cotten C,Rondeel JMM

    更新日期:2018-07-05 00:00:00

  • A Survey of Knowledge and Practices of Transfusion Medicine Among Post Intern Doctors in Specialized Hospital in Sri Lanka.

    abstract:BACKGROUND:Knowledge of transfusion medicine is the key element of better transfusion practices. This deficit observed at the blood bank end on daily basis exposing the patients for redundant risk. We assessed the knowledge of transfusion medicine among post intern doctors. OBJECTIVE:To assess the knowledge of transfu...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmw056

    authors: Kumarage S,Fernando R,Gunasekara L

    更新日期:2017-02-01 00:00:00

  • Post-Transfusion Purpura Mimicking Idiopathic Thrombocytopenic Purpura: A Case Report.

    abstract::The main clinical distinction between post-transfusion purpura (PTP) and idiopathic thrombocytopenic purpura (ITP) is the sudden development of severe thrombocytopenia in the days after transfusion. Herein, we report the case of a 53-year-old Caucasian woman who developed multiple myeloma (MM) after peripheral blood-s...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmz004

    authors: Milito C,Masel D,Henrichs K,Schmidt AE,Kirkley S,Aljitawi O,Becker M,Blumberg N,Refaai MA

    更新日期:2019-10-10 00:00:00

  • Prevalence and Clinical Utility of "Incidental" Critical Values Resulting From Critical Care Laboratory Testing.

    abstract:OBJECTIVE:Panels of clinical laboratory testing may generate "incidental" critical values from unordered parameters. Existing regulations do not clearly delineate guidelines for handling incidental critical values. The objective of this study was to examine the patterns and clinical utility of incidental critical value...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmw044

    authors: Grieme CV,Voss DR,Olson KE,Davis SR,Kulhavy J,Krasowski MD

    更新日期:2016-11-01 00:00:00

  • The Effectiveness of Temperature Versus Aprotinin in Maintaining the Preanalytical Stability of Adrenocorticotrophin.

    abstract:OBJECTIVE:To investigate the effect of temperature and protease inhibitor on adrenocorticotrophin (ACTH) stability. METHODS:We collected blood specimens from 10 donors into K3 EDTA (ethylenediaminetetraacetic acid) and K3 EDTA + aprotinin tubes. Plasma specimens were held at room temperature and at 2 °C to 8[Formula: ...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmw027

    authors: Turkon H,Toprak B,Yalcin H,Colak A,Ozturk N

    更新日期:2016-11-01 00:00:00

  • Blood collection from intravenous lines: is one drawing site better than others?

    abstract:OBJECTIVE:Establish whether hemolysis in samples collected from intravenous lines is influenced by catheterization site. METHODS:Blood was collected from all patients (67 total) admitted to the emergency department the same morning, through a 20-gauge catheter placed in a vein of the upper limb directly into an evacua...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1309/lm2xcv5sqml1ontm

    authors: Lippi G,Avanzini P,Aloe R,Cervellin G

    更新日期:2014-04-01 00:00:00

  • Drug-induced autoimmune hemolytic anemia in a 78-year-old African-American man with chronic lymphocytic leukemia.

    abstract:PATIENT:78-year-old African-American man. PAST MEDICAL HISTORY:Chronic lymphocytic leukemia first diagnosed in 2003, with a subsequent relapse in 2006 and another in 2010. HISTORY OF PRESENT ILLNESS:In late 2011, the patient was admitted to the hospital for cholelithiasis, at which time his treating physician inciden...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1309/LMLWQDB2Q6LS7VQG

    authors: Sartori A,Staley B,Skipper A

    更新日期:2014-07-01 00:00:00

  • Vitamin D Toxicity: A 16-Year Retrospective Study at an Academic Medical Center.

    abstract:Background:Interest in vitamin D has increased during the past 2 decades, with a corresponding increase in laboratory testing of 25-hydroxyvitamin D [25(OH)D]. The vast majority of specimens tested display normal or deficient levels of 25(OH)D; concentrations rarely fall in the potentially toxic range. Methods:We perf...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmx077

    authors: Lee JP,Tansey M,Jetton JG,Krasowski MD

    更新日期:2018-03-21 00:00:00

  • Ficin-Treated Red Cells Help Identify Clinically Significant Alloantibodies Masked as Reactions of Undetermined Specificity in Gel Microtubes.

    abstract::Non-specific antibodies or antibodies of undetermined significance (AUS) often pose problems for a blood bank technologist and physician. It is well known that antibodies can weaken and evanesce over time, thus eluding detection by routine blood bank techniques. Special enhancement techniques exist (eg, ficin treatmen...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmw062

    authors: Hill BC,Hanna CA,Adamski J,Pham HP,Marques MB,Williams LA 3rd

    更新日期:2017-02-01 00:00:00

  • Potential Use of Antigen-Based Rapid Test for SARS-CoV-2 in Respiratory Specimens in Low-Resource Settings in Egypt for Symptomatic Patients and High-Risk Contacts.

    abstract:OBJECTIVE:Because of the rapidly emerging SARS-CoV-2 pandemic and its wide public health challenges, rapid diagnosis is essential to decrease the spread. Antigen-based rapid detection tests are available; however, insufficient data about their performance are available. METHODS:The lateral-flow immunochromatographic B...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmaa104

    authors: Abdelrazik AM,Elshafie SM,Abdelaziz HM

    更新日期:2020-12-07 00:00:00

  • Tacrolimus: A Review of Laboratory Detection Methods and Indications for Use.

    abstract::Tacrolimus (Tac) is an immunosuppressive drug that is used in preventing organ and tissue rejection in patients after transplantation. Tac administration requires frequent and diligent monitoring by physicians to ensure proper dosage and to limit the potential for harmful adverse effects, which can include renal damag...

    journal_title:Laboratory medicine

    pub_type: 杂志文章,评审

    doi:10.1093/labmed/lmx056

    authors: Kalt DA

    更新日期:2017-11-08 00:00:00

  • Persistent Rivaroxaban Effect Due to Impaired Renal Clearance and Medication Effects.

    abstract::Rivaroxaban (Xarelto; Johnson & Johnson Services, Inc) is a direct oral anticoagulant (DOAC) that works by directly inhibiting the active site of factor Xa (FXa). Rivaroxaban is metabolized and cleared via the kidney and liver. The results of various studies have shown that patients with severe renal impairment should...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmz044

    authors: Milito C,McRae H,Victor A,Refaai MA,Schmidt AE

    更新日期:2020-03-10 00:00:00

  • Balamuthia mandrillaris-Related Primary Amoebic Encephalitis in China Diagnosed by Next Generation Sequencing and a Review of the Literature.

    abstract:BACKGROUND:Encephalitis is caused by infection, immune mediated diseases, or primary inflammatory diseases. Of all the causative infectious pathogens, 90% are viruses or bacteria. Granulomatous amoebic encephalitis (GAE), caused by Balamuthia mandrillaris, is a rare but life-threatening disease. Diagnosis and therapy a...

    journal_title:Laboratory medicine

    pub_type: 杂志文章,评审

    doi:10.1093/labmed/lmz079

    authors: Yang Y,Hu X,Min L,Dong X,Guan Y

    更新日期:2020-03-10 00:00:00

  • A New Indicator Derived From Reticulocyte Hemoglobin Content for Screening Iron Deficiency in an Area Prevalent for Thalassemia.

    abstract:OBJECTIVE:To establish a new indicator derived from reticulocyte hemoglobin (Ret-He) content and red blood cell (RBC) indices for screening for iron deficiency anemia (IDA) in an area in whch thalassemia is prevalent. METHODS:Blood specimens from 304 women aged between 18 and 30 years residing in northeast Thailand we...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmz099

    authors: Jamnok J,Sanchaisuriya K,Chaitriphop C,Sanchaisuriya P,Fucharoen G,Fucharoen S

    更新日期:2020-09-01 00:00:00

  • Following the Rules Set by Accreditation Agencies and Governing Bodies to Maintain In-Compliance Status: Applying Critical Thinking Skills When Evaluating the Need for Change in the Clinical Laboratory.

    abstract::Maintaining an in-compliance clinical laboratory takes continuous awareness and review of standards, regulations, and best practices. A strong quality assurance program and well informed leaders who maintain professional networks can aid in this necessary task. This article will discuss a process that laboratories can...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmw007

    authors: Byrne KM,Levy KY,Reese EM

    更新日期:2016-05-01 00:00:00

  • Disseminated Hormographiella aspergillata Infection with Lung and Brain Involvement after Allogenic Hematopoietic Stem-Cell Transplantation in a 54-Year-Old Man.

    abstract::Hormographiella is a rare fungal pathogen in humans; however, case reports have described disseminated infection in immunocompromised hosts. This pathogen has been described to yield poor prognosis in patients who harbor it. Herein, we present a case report of autopsy-proven disseminated Hormographiella aspergillata i...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmz018

    authors: Chauhan A,Gruenberg J,Arbefeville S,Mettler T,Brent CH,Ferrieri P

    更新日期:2019-10-10 00:00:00

  • Accidental diagnosis: Aeromonas infection leading to discovery of acute myeloid leukemia with meylodysplastic syndrome.

    abstract:PATIENT:47-year-old African American man. CHIEF COMPLAINT:Initially, serious finger abscess. The patient reported severe pain that was localized to that finger only. HISTORY OF PRESENT ILLNESS:His finger had become sore and then progressively more painful and swollen. PAST MEDICAL HISTORY:A few weeks earlier, the pa...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1309/LMX711TANCVDHZJJ

    authors: Rausch S

    更新日期:2014-07-01 00:00:00