Quantitation of Cell-Derived Microparticles in Blood Products and Its Potential Applications in Transfusion Laboratories.

abstract：OBJECTIVE:To evaluate broad-range 16S ribosomal DNA (rDNA) polymerase chain reaction (PCR) as a rapid screening tool to detect bacterial contamination of stem-cell products. METHODS:We performed the evaluation using whole blood spiked with serially diluted bacterial-type strains. Detection sensitivity was defined as t...

journal_title：Laboratory medicine

authors： Tokuno O,Hayakawa A,Yanai T,Mori T,Ohnuma K,Tani A,Minami H,Sugimoto T

更新日期：2015-01-01 00:00:00

abstract：:Storage pool deficiency (SPD) is a group of rare platelet disorders that result from deficiencies in α-granules, δ-granules, or both. One type of α-SPD is gray platelet syndrome (GPS), caused by mutations in the neurobeachin-like 2 (NBEAL2) gene that results in a bleeding diathesis, thrombocytopenia, splenomegaly, and...

journal_title：Laboratory medicine

authors： Riley R,Khan A,Pai S,Warmke L,Winkler M,Gunning W

更新日期：2019-10-10 00:00:00

abstract：BACKGROUND:To compare the coagulation-factor profile of cryoprecipitate produced from fresh frozen plasma from whole blood (WB) stored for 24 hours at room temperature (24CP) with that of standard cryoprecipitate (CP). METHODS:We collected 80 units of WB from healthy volunteers, of which 20 units were of each blood gr...

journal_title：Laboratory medicine

authors： Philip J,Kumarage S,Chatterjee T,Kumar S,Mallhi R

更新日期：2014-04-01 00:00:00

abstract：Background:Thromboelastography (TEG) has become the standard of care in liver-transplant surgery to identify real-time abnormalities in the coagulation cascade. To our knowledge, no studies have been performed to measure the intrasubject reproducibility of TEG parameters in cirrhotic patients. Objective:To perform a v...

journal_title：Laboratory medicine

authors： George G,Manatasahit W,Balasubramanian M,Navarro V

更新日期：2018-07-05 00:00:00

abstract：:Cording is a phenomenon in which acid fast bacilli grow in parallel and was previously used as a means of presumptive microscopic identification of Mycobacterium tuberculosis (TB). However, this process has been shown in multiple other nontuberculous mycobacterial (NTM) species. Here we present the case of an immunoco...

journal_title：Laboratory medicine

authors： Olson G,McNulty MC,Mullane K,Beavis KG,Tesic V

更新日期：2020-09-21 00:00:00

abstract：:The COVID-19 pandemic has led to a major setback in both the health and economic sectors across the globe. The scale of the problem is enormous because we still do not have any specific anti-SARS-CoV-2 antiviral agent or vaccine. The human immune system has never been exposed to this novel virus, so the viral interact...

journal_title：Laboratory medicine

authors： Sahu KK,Siddiqui AD,Cerny J

更新日期：2021-01-04 00:00:00

abstract：OBJECTIVE:To investigate the expression level and clinical significance of microRNA-155 (miR-155) in bladder cancer. METHODS:We collected 102 pairs of tissue specimens from patients with primary bladder cancer and adjacent normal bladder specimens between March 2008 and May 2013. Quantitative real-time polymerase chai...

journal_title：Laboratory medicine

authors： Wang H,Men CP

更新日期：2015-04-01 00:00:00

abstract：:Extremely low hemoglobin A1c (HbA1c) results below reference range are rare, and the causes and clinical implications associated with low HbA1c results are not well understood among clinical laboratorians. A case of extremely low HbA1c results was reported, in which liver cirrhosis, subacute hemorrhage and recent tran...

journal_title：Laboratory medicine

authors： Wang P

更新日期：2017-02-01 00:00:00

abstract：OBJECTIVE:Establish whether hemolysis in samples collected from intravenous lines is influenced by catheterization site. METHODS:Blood was collected from all patients (67 total) admitted to the emergency department the same morning, through a 20-gauge catheter placed in a vein of the upper limb directly into an evacua...

journal_title：Laboratory medicine

authors： Lippi G,Avanzini P,Aloe R,Cervellin G

更新日期：2014-04-01 00:00:00

abstract：OBJECTIVE:Inflammation of the small intestine may occur in type 2 diabetes. This study aimed to investigate whether ATP-binding cassette transporter A1 (ABCA1) and G1 (ABCG1) were altered in chronic inflammation of the small intestine of type 2 diabetic rats. METHODS:Thirty-two male Sprague-Dawley rats were used. Eigh...

journal_title：Laboratory medicine

authors： Lou J,Zhou H,Li C,Hu L,Lu X,Li J,Yao H,Li W,Zhang X,Xu M

更新日期：2014-01-01 00:00:00

abstract：BACKGROUND:Encephalitis is caused by infection, immune mediated diseases, or primary inflammatory diseases. Of all the causative infectious pathogens, 90% are viruses or bacteria. Granulomatous amoebic encephalitis (GAE), caused by Balamuthia mandrillaris, is a rare but life-threatening disease. Diagnosis and therapy a...

journal_title：Laboratory medicine

authors： Yang Y,Hu X,Min L,Dong X,Guan Y

更新日期：2020-03-10 00:00:00

abstract：BACKGROUND:Preparation of the patient for laboratory tests is crucial. Our aim was to investigate the current practice and regional variability of recommendations regarding patient preparation for laboratory testing. METHODS:A call for data was posted by email. Spanish laboratories were invited to fill out and submit ...

journal_title：Laboratory medicine

authors： Salinas M,López-Garrigós M,Flores E,Leiva-Salinas C

更新日期：2020-05-06 00:00:00

abstract：:We report a case of a false-positive HIV result in an apparently healthy pregnant woman. Since no prenatal HIV testing had been performed, we screened for HIV reactivity utilizing the Architect HIV-Ag/Ab Combo assay. Results obtained were inconsistent in that they were repeatedly HIV reactive on a single serum sample ...

journal_title：Laboratory medicine

authors： Akl P,Blick KE

更新日期：2014-07-01 00:00:00

abstract：OBJECTIVE:Examination of urine sediment is crucial in acute kidney injury (AKI). In such renal injury, tubular epithelial cells, epithelial cell casts, and dysmorphic red cells may provide clues to etiology. The aim of this study was to compare automated urinalysis findings with manual microscopic analysis in AKI. MET...

journal_title：Laboratory medicine

authors： Chandrashekar V,Tarigopula A,Prabhakar V

更新日期：2020-10-03 00:00:00

abstract：BACKGROUND:Consanguineous marriage, a common practice in South India, increase the incidence of autosomal recessive diseases such as nonsyndromic hearing loss (NSHL) in offspring. This trend was noted in the children with hearing impairment (HI) who received cochlear implants (CI) at our University hospital in Porur, C...

journal_title：Laboratory medicine

authors： Arunachalam RK,Koshy T,Venkatesan V,Dawson GP,Franklin Durairaj Paul S,George P

更新日期：2020-01-02 00:00:00

abstract：BACKGROUND:Research in several professional fields has demonstrated that delays (time lapse) in taking certification examinations may result in poorer performance by examinees. Thirteen states and/or territories require licensure for laboratory personnel. A core component of licensure is passing a certification exam. A...

journal_title：Laboratory medicine

authors： ASCP Board of Certification Research and Development Committee.,Brown KA,Fenn JP,Freeman VS,Fisher PB,Genzen JR,Goodyear N,Houston ML,O'Brien ME,Tanabe PA

更新日期：2015-07-01 00:00:00

abstract：OBJECTIVE:Platelet counts generated by automated analyzers on blood specimens that contain platelet clumps are often inaccurate and require verification by blood-smear review. In this study, we assessed the reliability of the Sysmex XE-5000 instrument to detect platelet clumps. METHOD:We reviewed automated complete bl...

journal_title：Laboratory medicine

authors： Hawkins J,Gulati G,Uppal G,Gong J

更新日期：2016-08-01 00:00:00

abstract：OBJECTIVE:To identify the osteoprotegerin (OPG) correlates with antiphospholipid syndrome (APS) parameters. METHODS:Our cohort included 40 patients with primary APS disease associated with systemic lupus erythematosus (SLE) (mean age, 43.7 years; 87% female). Data on cardiovascular risk factors and specific clinical e...

journal_title：Laboratory medicine

authors： Caraiola S,Dima A,Jurcut C,Jurcut R,Baicus C,Baicus A

更新日期：2020-03-10 00:00:00

abstract：:The three major causes of anemia in neonates are blood loss, decreased red blood cell production, and increased degradation of erythrocytes. Establishing the cause of anemia in a neonate born prematurely can be challenging. Clinically, fetomaternal hemorrhage (FMH) can be difficult to diagnose-the condition often pres...

journal_title：Laboratory medicine

authors： den Besten G,van der Weide K,Schuerman FABA,Michael Cotten C,Rondeel JMM

更新日期：2018-07-05 00:00:00

abstract：OBJECTIVE:To explore novel biomarkers for patients with pancreatic ductal adenocarcinoma (PDAC), from the perspective of tumor hypoxia. METHODS:We screened 29 differentially expressed and hypoxia-upregulated genes from the Oncomine database. A total of 12 secretory proteins that interact with hypoxia-inducible factor ...

journal_title：Laboratory medicine

authors： Yang L,Cui R,Li Y,Liang K,Ni M,Gu Y

更新日期：2020-07-08 00:00:00

abstract：Background:The misuse of statistical methods in diagnostic accuracy studies has been criticized in many publications. Objective:To assess the use and misuse of statistical methods in medical journal articles. Methods:We reviewed 141 original articles from 6 cytopathology journals published in 2014. Results:In total,...

journal_title：Laboratory medicine

authors： Bahar B,Pambuccian SE,Barkan GA,Akdas Y

更新日期：2019-01-01 00:00:00

abstract：:Rivaroxaban (Xarelto; Johnson & Johnson Services, Inc) is a direct oral anticoagulant (DOAC) that works by directly inhibiting the active site of factor Xa (FXa). Rivaroxaban is metabolized and cleared via the kidney and liver. The results of various studies have shown that patients with severe renal impairment should...

journal_title：Laboratory medicine

authors： Milito C,McRae H,Victor A,Refaai MA,Schmidt AE

更新日期：2020-03-10 00:00:00

abstract：BACKGROUND:Multirules are often employed to monitor quality control (QC). The performance of multirules is usually determined by simulation and is difficult to predict. Previous studies have not provided computer code that would enable one to experiment with multirules. It would be helpful for analysts to have computer...

journal_title：Laboratory medicine

authors： Walker BS,Pearson LN,Schmidt RL

更新日期：2020-01-02 00:00:00

abstract：BACKGROUND:The University of Maryland Medical System, like all other medical systems, is faced with providing the highest quality care in an environment of increased demands and competition for resources. Some responses to this pressure include improved integration, coordination, and efficiency. Laboratory integration ...

journal_title：Laboratory medicine

authors： Cook J

更新日期：2017-02-01 00:00:00

abstract：OBJECTIVE:To investigate the effect of temperature and protease inhibitor on adrenocorticotrophin (ACTH) stability. METHODS:We collected blood specimens from 10 donors into K3 EDTA (ethylenediaminetetraacetic acid) and K3 EDTA + aprotinin tubes. Plasma specimens were held at room temperature and at 2 °C to 8[Formula: ...

journal_title：Laboratory medicine

authors： Turkon H,Toprak B,Yalcin H,Colak A,Ozturk N

更新日期：2016-11-01 00:00:00

abstract：:Hormographiella is a rare fungal pathogen in humans; however, case reports have described disseminated infection in immunocompromised hosts. This pathogen has been described to yield poor prognosis in patients who harbor it. Herein, we present a case report of autopsy-proven disseminated Hormographiella aspergillata i...

journal_title：Laboratory medicine

authors： Chauhan A,Gruenberg J,Arbefeville S,Mettler T,Brent CH,Ferrieri P

更新日期：2019-10-10 00:00:00

abstract：BACKGROUND:Single-vial fecal immunochemical testing (FIT) is an accepted method of colorectal cancer (CRC) screening. The available 3-vial FIT data set allows for comparison of colonoscopy results using various screening methods. OBJECTIVE:To determine the optimal number of vials for a strong FIT-screening program by ...

journal_title：Laboratory medicine

authors： Hancock JA,Palmer GA

更新日期：2019-07-16 00:00:00

abstract：BACKGROUND:Knowledge of transfusion medicine is the key element of better transfusion practices. This deficit observed at the blood bank end on daily basis exposing the patients for redundant risk. We assessed the knowledge of transfusion medicine among post intern doctors. OBJECTIVE:To assess the knowledge of transfu...

journal_title：Laboratory medicine

authors： Kumarage S,Fernando R,Gunasekara L

更新日期：2017-02-01 00:00:00

abstract：:Myeloperoxidase (MPO) deficiency, one of the most common inherited phagocyte defects, and may exist as a transient phenomenon in combination with some clinical condition. Hematological analyzer ADVIA 2120i is used to identify the different types of leukocytes based on their size and staining properties, and by mean pe...

journal_title：Laboratory medicine

authors： Roh S,Ham JY,Song KE,Hwang N,Lee NY

更新日期：2020-03-10 00:00:00

abstract：:Tacrolimus (Tac) is an immunosuppressive drug that is used in preventing organ and tissue rejection in patients after transplantation. Tac administration requires frequent and diligent monitoring by physicians to ensure proper dosage and to limit the potential for harmful adverse effects, which can include renal damag...

journal_title：Laboratory medicine

authors： Kalt DA

更新日期：2017-11-08 00:00:00