Abstract:
UNLABELLED:Chronic liver diseases may cause inflammation and progressive scarring, over time leading to irreversible hepatic damage (cirrhosis). As a result, the need to assess and closely monitor individuals for risk factors of components of matrix deposition and degradation, as well as the severity of the fibrosis using biomarkers, has been increasingly recognized. AIM:Our aim is to review the use of biomarker for diagnosing and defining the severity of liver fibrosis. METHODS:A systematic literature review was done using the terms "hyaluronic acid" and "liver fibrosis" as well as the name of each biomarker or algorithm known to be employed. PubMed and Google Scholar were searched, and English language articles indexed between January 2010 and October 2014 in which HA was used as a marker of liver fibrosis were retrieved, regardless of the underlying liver disease. Each author read the publications separately and the results were analyzed and discussed. RESULTS:Biomarkers offer a potential prognostic or diagnostic indicator for disease manifestation, progression, or both. Serum biomarkers, including HA, have been used for many years. Emerging biomarkers such as metalloproteinases have been proposed as tools that provide valuable complementary information to that obtained from traditional biomarkers. Moreover, markers of extracellular matrix degradation provide powerful predictions of risk. In order for biomarkers to be clinically useful in accurately diagnosing and treating disorders, age-specific reference intervals that account for differences in gender and ethnic origin are a necessity. CONCLUSIONS:This review attempts to provide a comprehensive analysis of the emerging risk biomarkers of liver fibrosis and to describe the clinical significance and analytical considerations of each biomarker pointing out sentinel features of disease progression.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Neuman MG,Cohen LB,Nanau RMdoi
10.1016/j.clinbiochem.2015.07.019subject
Has Abstractpub_date
2016-02-01 00:00:00pages
302-15issue
3eissn
0009-9120issn
1873-2933pii
S0009-9120(15)00280-5journal_volume
49pub_type
杂志文章,评审abstract:CONTEXT:Knee arthroplasty surgery is significant trauma, leading to an activated immune system causing inflammation and oxidative stress. Many current biomarkers are invasive, costly, and often slow to analyse, limiting their use for rapid inflammatory measurements. OBJECTIVES:We have examined the use of urinary neopt...
journal_title:Clinical biochemistry
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1016/j.clinbiochem.2018.11.002
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVES:This study examines the redox status of drug-resistant epileptic patients and how it is modified after surgical treatment. DESIGN AND METHODS:The activity of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase), and markers of damage to biomolecules (malondialdehyde and advanced o...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2006.11.019
更新日期:2007-03-01 00:00:00
abstract:OBJECTIVE:The purpose of this study was to develop an improved method of direct DNA sequencing, which makes it possible to identify heterozygous mutations of the lipoprotein lipase (LPL) gene in order to understand the underlying genetic disorder of type IV hyperlipoproteinemia. METHODS AND RESULTS:The direct sequenci...
journal_title:Clinical biochemistry
pub_type: 临床试验,杂志文章
doi:10.1016/s0009-9120(97)00005-2
更新日期:1997-06-01 00:00:00
abstract:OBJECTIVES:To investigate the polymorphism distribution of Val-9Ala and Ile+58Thr of the Mn-superoxide dismutase (Mn-SOD) gene among subjects with Parkinson's disease (PD) by analyses of genders and clinical severity. DESIGN AND METHODS:We examined the DNA genotypes of Val-9Ala and Ile+58Thr from 295 PD subjects and 1...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2010.05.009
更新日期:2010-08-01 00:00:00
abstract::Two studies are described in this paper for the purpose of extending the application of the Dupont ACA glucose method to (1) analysis of specimens containing sodium fluoride and sodium iodoacetate, and (2) analysis of specimens containing EDTA, provided analysis is conducted immediately following specimen collection a...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(82)96520-1
更新日期:1982-04-01 00:00:00
abstract:OBJECTIVES:In widely used protocols for the collection and isolation of cord blood mononuclear cells, investigators are left with substantial volumes of diluted plasma which could be used for other measurements. The aim of this study was to ascertain the validity of umbilical cord blood (UCB) diluted plasma samples for...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2015.04.014
更新日期:2015-11-01 00:00:00
abstract:BACKGROUND:Vitamin D (25-OHD) physiological functions have been expanded beyond traditional bone health, increasing the importance of its estimation in Laboratory Medicine, which renders validation of available methods mandatory. AIMS AND METHODS:We evaluated some preanalytical and analytical aspects of 25-OHD determi...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2012.08.003
更新日期:2012-12-01 00:00:00
abstract::Autosomal dominant olivopontocerebellar degeneration was evaluated in a family numbering 414 living members. Urine samples were obtained from 5 affected and 5 unaffected not at-risk family members after 4 days on a synthetic diet. 159 urine vapor constituents were measured by gas-liquid and ion-exchange chromatography...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(75)93752-2
更新日期:1975-12-01 00:00:00
abstract:OBJECTIVES:Measurements of myoglobin and creatine kinase (CK)-MB isoforms have been suggested to be sensitive tests for the early diagnosis of myocardial infarction (MI). We have investigated the utility of myoglobin, creatine kinase (CK)-MB isoforms and creatine kinase MB mass (CK-MBm) in early diagnosis of MI using c...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(02)00385-5
更新日期:2002-11-01 00:00:00
abstract:OBJECTIVES:To compare HPLC methods with short and long elution times for HbA1c measurement in blood. METHODS:Comparison between G7-Tosoh (1.2 min); Bio-Rad-Variant-II-Turbo (1.3 min) and Arkray-HA-8160 (2.9 min). RESULTS:Passing-Bablok regression equations were: Y=0.17+0.96X; Y=-0.39+1.01X; Y=-0.40+1.0X and the means...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2009.07.003
更新日期:2009-10-01 00:00:00
abstract:OBJECTIVES:To evaluate the evidence concerning gFOBT kits and to develop gFOBT standards to be implemented by the Ontario Colorectal Cancer (CRC) Screening Program (the "Program"). DESIGN AND METHODS:We evaluated 3 aspects of gFOBT kits: 1) performance factors; 2) usability factors; and 3) laboratory factors. We obtai...
journal_title:Clinical biochemistry
pub_type: 杂志文章,meta分析
doi:10.1016/j.clinbiochem.2008.08.069
更新日期:2008-11-01 00:00:00
abstract:OBJECTIVES:Recurrent miscarriage (RM) is a challenging medical problem because of its unknown pathogenesis and etiology in most of the cases. Recent studies suggest the role of persistent environmental pollutants such as organochlorine pesticides (OCPs) in the etiology of RM. The present study was conducted to investig...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2009.09.019
更新日期:2010-01-01 00:00:00
abstract::We describe a fast and efficient method for the isolation of drugs from blood serum by use of the DU PONT PREP I Automated Sample Processor and octadecyl bonded phase columns. Drugs are selectively adsorbed onto C18 bonded phase and eluted with methanol. The recovered residue is essentially free of sample matrix chrom...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(81)91245-5
更新日期:1981-08-01 00:00:00
abstract:OBJECTIVE:Plasma ammonia measurement is greatly influenced by pre-analytical conditions, which may lead to false positives. We wanted to evaluate the prevalence and clinical impact of plasma ammonia false positives in a pediatric population. DESIGN AND METHODS:Over a 28-month period, charts of patients with elevated a...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2007.01.024
更新日期:2007-05-01 00:00:00
abstract::An immunosuppressive substance was isolated from ascitic fluids of patients with advanced colon cancer by means of ammonium sulphate precipitation and preparative isoelectric focusing. This was a glycoprotein with an isoelectric point of pH 2.7-3.3, a molecular weight of about 52,000, and a sedimentation coefficient o...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(87)80118-2
更新日期:1987-06-01 00:00:00
abstract::Macro creatine kinase type 2 (MCK-2) is a promising new tumour marker for carcinoma of the colon. We have purified it from tumour tissue, from serum and, for the first time, from normal colonic epithelium and identified it in a tissue culture line of colon cancer cells. The molecular weight of each preparation, regard...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(85)80071-0
更新日期:1985-12-01 00:00:00
abstract:OBJECTIVES:Personalized ranges of liver fibrosis serum biomarkers such as FibroTest or hyaluronic acid could be used for early detection of fibrotic changes in patients with progressive chronic liver disease. Our aim was to generate reliable biological variation estimates for these two biomarkers with confidence interv...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2015.03.020
更新日期:2015-07-01 00:00:00
abstract:OBJECTIVES:Most clinical laboratories replaced their manual precipitation techniques for the determination of urinary protein with automated dye binding assays or benzethonium chloride-turbidimetric assays. Few studies have validated these assays for the measurement of urinary proteins in the normal range. DESIGN AND ...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2004.12.010
更新日期:2005-05-01 00:00:00
abstract:OBJECTIVES:NGAL (Neutrophil Gelatinase-Associated Lipocalin) has emerged as a new biomarker for the identification of acute kidney injury. Reliable clinical evaluations require a simple, robust test method for NGAL, and knowledge of specimen handling and specimen stability characteristics. We evaluated the performance ...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2009.12.008
更新日期:2010-04-01 00:00:00
abstract::Technological developments and economic drivers have led to major changes in clinical laboratories worldwide, principally represented by an increase in testing productivity and efficiency. Currently, major expectations are entrusted on the introduction of diagnostically more effective tests as well as on translating t...
journal_title:Clinical biochemistry
pub_type: 社论
doi:10.1016/j.clinbiochem.2010.05.015
更新日期:2010-08-01 00:00:00
abstract:BACKGROUND:With the advent of the new high-sensitivity troponin assays, it is becoming critical to measure troponin accurately to low concentrations. To ensure assay performance is acceptable, appropriate QC must be run. METHODS:In addition to the routine use of commercial QC material, we prepared pools of human QC ma...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2018.05.007
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVE:To determine Retinol Binding Protein 4 (RBP4) levels in patients with Familial Partial Lipodystrophy (FPLD). METHODS:Ten patients with FPLD and a control group (9 patients) were selected to participate in the study. RESULTS:RBP4-log levels were lower in patients with FPLD in comparison to control group (1.5...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2009.03.006
更新日期:2009-07-01 00:00:00
abstract:OBJECTIVE:Prostate-specific antigen (PSA) samples from five commercial sources were compared with PSA from the European Commission Community Bureau of Reference, known as CRM 613, using liquid chromatography-mass spectrometry (LC-MS) and matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS). DESIGN ...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2004.10.016
更新日期:2005-02-01 00:00:00
abstract:UNLABELLED:Antioxidant capacity testing is commonly used in clinical investigations to provide an estimate of in vitro antioxidant capacity of biosamples. Although beneficial to measure the synergistic contribution of all compounds with antioxidant functionality, assessing the capacity of non-protein fractions or small...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2014.06.021
更新日期:2014-10-01 00:00:00
abstract:OBJECTIVES:Despite the well-defined genetics of FMF, limited information is available regarding the regulation of inflammation by cytokines. DESIGN AND METHODS:The levels of systemic cytokines and other markers of inflammation in FMF patients and control were measured by ELISA and Cytometric Bead Array (CBA). RESULTS...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2008.03.017
更新日期:2008-07-01 00:00:00
abstract:OBJECTIVES:This study investigates how some of human physiological parameters, in particular sex and daytime, can contribute to the normal variation in blood gene expression. DESIGN AND METHODS:The expressions of two genes: SOD1 (Cu/Zn) and SOD2 (Mn) in white blood cells of 19 healthy individuals (10 men and 9 women) ...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2008.08.064
更新日期:2008-11-01 00:00:00
abstract:OBJECTIVES:The use of iodinated contrast media has grown in popularity in the past two decades, but relatively little attention has been paid to the possible interferential effects of contrast media on laboratory test results. Herein, we investigate medical contrast media interference with routine chemistry results obt...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2017.02.023
更新日期:2017-08-01 00:00:00
abstract:OBJECTIVES:To ascertain the effects of eating on plasma antioxidant capacity in patients with liver disease. DESIGN AND METHODS:Eighteen cirrhotic patients were compared to 18 age and sex-matched controls. TRAP was measured by a fluorometric assay after a 12 h fast, and 60, 120, and 180 min after the study participant...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(99)00039-9
更新日期:1999-08-01 00:00:00
abstract:OBJECTIVES:Bisphenol A (BPA) exposure may promote obesity, but its effect on bone mineral density (BMD) has not been reported in humans. We aimed to examine the relationships between BPA exposure, body composition, serum estradiol, leptin, osteocalcin levels and BMDs in healthy premenopausal women. DESIGN AND METHODS:...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2012.08.024
更新日期:2012-12-01 00:00:00
abstract:OBJECTIVES:To evaluate the comparative chemopreventive efficacy of green tea polyphenols (Polyphenon-E) and black tea polyphenols (Polyphenon-B) on 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis. DESIGN AND METHODS:Hamsters were divided into 6 groups. Animals in group 1 served ...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2005.06.011
更新日期:2005-10-01 00:00:00