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Influence of reduction in the elution times on HPLC glycohaemoglobin results.

Abstract：

OBJECTIVES:To compare HPLC methods with short and long elution times for HbA1c measurement in blood. METHODS:Comparison between G7-Tosoh (1.2 min); Bio-Rad-Variant-II-Turbo (1.3 min) and Arkray-HA-8160 (2.9 min). RESULTS:Passing-Bablok regression equations were: Y=0.17+0.96X; Y=-0.39+1.01X; Y=-0.40+1.0X and the means of the differences using Bland-Altman Plot were 0.02; -0.34; 0.32 for HA-8160/G7-Tosoh, HA-8160/Variant-II-Turbo and G7-Tosoh/Variant-II-Turbo, respectively. CONCLUSIONS:Faster elution methods had no problems on reproducibility of results obtained by slower elution methods.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Leon-Justel A,Santotoribio JD,Dominguez-Pascual I,Delgado AL,Macias C,Herrera MT,Leal A,Mangas MA,Acosta D,Guerrero JM

doi

10.1016/j.clinbiochem.2009.07.003

subject

Has Abstract

pub_date

2009-10-01 00:00:00

pages

1582-4

issue

15

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(09)00296-3

journal_volume

42

pub_type

杂志文章

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