Evaluation of the Boehringer Mannheim ES 300 immunoassay analyzer and comparison with enzyme immunoassay, fluorescence polarization immunoassay, and radioimmunoassay methods.

abstract：OBJECTIVES:To evaluate the analytical performance of the testosterone assay performed on the Bayer immuno 1 system from Bayer Corporation. DESIGN AND METHODS:The precision was measured using three Bayer TESTpoint Ligand controls, three Medical Decision Pools and the Bayer SETpoint Testosterone calibrators. The lineari...

journal_title：Clinical biochemistry

authors： Lévesque A,Letellier M,Swirski C,Lee C,Grant A

更新日期：1998-02-01 00:00:00

abstract：OBJECTIVES:Salivary analysis is a noninvasive alternative that may be more acceptable to patients, especially children. As such, it has the potential for incorporation into comprehensive, dynamic investigations of metabolic dysfunctions - a significant advancement over a single time point serum analysis. In this study,...

journal_title：Clinical biochemistry

authors： Chiu SK,Collier CP,Clark AF,Wynn-Edwards KE

更新日期：2003-05-01 00:00:00

abstract：OBJECTIVE:To develop sensitive nonisotopic assays for protein-tyrosine phosphatase (PTP) activity. METHODS:The fluorometric assay is based on the fact that phosphotyrosine but not tyrosine forms highly fluorescent complexes with Tb3+. Thus, PTP activity can be followed by measuring the decrease of fluorescence due to ...

journal_title：Clinical biochemistry

authors： Galvan B,Christopoulos TK

更新日期：1996-04-01 00:00:00

abstract：BACKGROUND:The p53 gene is an established tumor suppressor and an inducer of apoptosis. We here attempt to determine whether the putative anticarcinogenic properties attributed to red wine and its polyphenolic constituents depend, at least in part, upon their ability to modulate p53 expression in cancer cells. METHODS...

journal_title：Clinical biochemistry

authors： Soleas GJ,Goldberg DM,Grass L,Levesque M,Diamandis EP

更新日期：2001-07-01 00:00:00

abstract：:We describe a patient who received intravenous dimethyl sulphoxide (DMSO) (as part of an experimental protocol) to control increased intracranial pressure. The patient died of a brain abscess. She developed extreme serum hyperosmolality from the DMSO with the osmolality peaking at 717 mosmol/kg. DMSO may induce extrem...

journal_title：Clinical biochemistry

authors： Wolf P,Simon M

更新日期：1983-08-01 00:00:00

abstract：:Cyclosporine A (CsA) represents one of the more important therapeutic advances in the field of kidney transplantation. However, its effectiveness is limited by serious side effects, most notably nephrotoxicity. Investigation of the mechanisms of CsA-induced renal dysfunction has been hampered by the lack of a suitable...

journal_title：Clinical biochemistry

authors： Thliveris JA,Yatscoff RW,Lukowski MP,Copeland KR

更新日期：1991-02-01 00:00:00

abstract：:This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...

journal_title：Clinical biochemistry

authors： Delvin EE

更新日期：2006-04-27 00:00:00

abstract：:A commercially available assay (Sigma) for urinary oxalates based on the oxalate oxidase/peroxidase catalyzed reaction produced, in our hands, recoveries of added oxalate that varied from patient to patient. A modification of the assay is reported that involves the chromatographic separation of interfering substances,...

journal_title：Clinical biochemistry

authors： Mazzuchin A,Michelutti L,Falter H

更新日期：1990-04-01 00:00:00

abstract：OBJECTIVES:This study examines the redox status of drug-resistant epileptic patients and how it is modified after surgical treatment. DESIGN AND METHODS:The activity of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase), and markers of damage to biomolecules (malondialdehyde and advanced o...

journal_title：Clinical biochemistry

authors： López J,González ME,Lorigados L,Morales L,Riverón G,Bauzá JY

更新日期：2007-03-01 00:00:00

abstract：OBJECTIVES:To develop a method for the determination of succinylacetone (SA) in dried blood spots (DBS) using tandem mass spectrometry (MS/MS). METHODS:SA was extracted from DBS with an acetonitrile and water solution (80:20 by volume) containing formic acid and hydrazine hydrate (both at 0.1% by volume), and analyzed...

journal_title：Clinical biochemistry

authors： Allard P,Grenier A,Korson MS,Zytkovicz TH

更新日期：2004-11-01 00:00:00

abstract：OBJECTIVES:Familial hypercholesterolemia (FH) is characterized by increased oxidative stress (OS) levels. In the postprandial state, lipids and lipoproteins modulate OS status through their impact on pro-oxidant and antioxidant mechanisms. The objective of this study was to evaluate in patients with FH the response to ...

journal_title：Clinical biochemistry

authors： Cortes R,Martinez-Hervas S,Ivorra C,De Marco G,Gonzalez-Albert V,Rojo-Martínez G,Saez G,Carmena R,Ascaso JF,Real JT,Chaves FJ

更新日期：2014-12-01 00:00:00

abstract：OBJECTIVES:It has recently been suggested that when adjusting doses of cyclosporine (CsA), determining its concentration in blood samples taken 2 h postdose (C(2)) is more clinically beneficial than using the predose concentration (C(0)). We determined C(0) and C(2) concentrations of CsA and their metabolites in sample...

journal_title：Clinical biochemistry

authors： Fernández-Marmiesse A,Hermida J,Tutor JC

更新日期：2000-07-01 00:00:00

abstract：:Plasma levels of dehydroepiandrosterone sulfate, dehydroepiandrosterone and delta 5-androstenediol were measured in several women throughout pregnancy. The levels of both dehydroepiandrosterone and its sulfate decreased while those of delta 5-androstenediol did not. The levels of binding of the latter steroid to plasm...

journal_title：Clinical biochemistry

authors： Bird CE,Cook SJ,Dressel DE,Clark AF

更新日期：1980-06-01 00:00:00

abstract：UNLABELLED:Chronic liver diseases may cause inflammation and progressive scarring, over time leading to irreversible hepatic damage (cirrhosis). As a result, the need to assess and closely monitor individuals for risk factors of components of matrix deposition and degradation, as well as the severity of the fibrosis us...

journal_title：Clinical biochemistry

authors： Neuman MG,Cohen LB,Nanau RM

更新日期：2016-02-01 00:00:00

abstract：:Stenosis is a symptom of coronary artery disease (CAD), and is caused by narrowing of arteries in the heart. Over the last several decades, medical implants such as cardiac stents have been developed to counter stenosis. Upon implantation of a stent to open up a restricted artery, narrowing of the artery can reoccur (...

journal_title：Clinical biochemistry

authors： Ravindranath RR,Romaschin A,Thompson M

更新日期：2016-01-01 00:00:00

abstract：OBJECTIVE:Elevated concentrations of hCG beta core fragment (hCGβcf) are known to cause false-negative results in qualitative urine pregnancy test devices, but the pattern of urinary hCGβcf during normal pregnancy has not been well characterized. Here, we evaluate the relationship between urine hCG, hCGβcf, and hCG fre...

journal_title：Clinical biochemistry

authors： Nerenz RD,Yarbrough ML,Stenman UH,Gronowski AM

更新日期：2016-07-01 00:00:00

abstract：OBJECTIVE:The aim of this study was to find a pre-interventional marker with the capacity to predict in-stent restenosis (ISR). Considering the anti-atherosclerotic role of adiponectin (APO), an adipocytokine with anti-inflammatory, anti-proliferative, anti-oxidative and anti-thrombotic properties, low plasma levels of...

journal_title：Clinical biochemistry

authors： Moldoveanu E,Mut-Vitcu B,Tanaseanu GR,Marta DS,Manea G,Kosaka T,Vidulescu C,Tanaseanu C

更新日期：2008-12-01 00:00:00

abstract：:To design and deliver high quality, safe and effective products, manufacturers of in vitro diagnostic (IVD) products follow a structured, traceable process for controlling the uncertainty of results reported from their measurement systems. This process and its results however, are not often shared in detail with those...

journal_title：Clinical biochemistry

authors： Orzechowski A,Petrides V,Scopp R

更新日期：2018-07-01 00:00:00

abstract：:In hepatobiliary disease, the level of bile acids in the peripheral circulation is greatly increased due to impaired liver function. It has been suggested that serum bile acids are of greater value in confirming hepatobiliary dysfunction than the currently assayed conventional biochemical parameters. Fifty patients wi...

journal_title：Clinical biochemistry

authors： Jarzabek JI,Kampa IS

更新日期：1981-12-01 00:00:00

abstract：OBJECTIVE:We used single-strand conformational polymorphism (SSCP) to screen for mutations at nucleotides 833 and 919 of the cystathionine beta-synthase (CBS) gene in 13 patients with homocystinuria and 11 of their relatives. METHODS:Exon 8 of genomic DNA was selectively amplified by PCR using primers derived from int...

journal_title：Clinical biochemistry

authors： Tsai MY,Hanson NQ,Bignell MK,Schwichtenberg KA

更新日期：1996-10-01 00:00:00

abstract：OBJECTIVES:In a previous publication, we had presented a sensitive method to detect mutations of the segment of the human glucocorticoid receptor alpha (hGRalpha) gene encoding the ligand binding domain (LBD) and part of the DNA binding domain (DBD) of hGRalpha, as several types of glucocorticoid resistance syndromes h...

journal_title：Clinical biochemistry

authors： Tsolakidou AF,Coulocheri SA,Sekeris CE,Moutsatsou P

更新日期：2003-06-01 00:00:00

abstract：OBJECTIVES:Receptor for advanced glycation end products (RAGE) plays a role in inflammatory reactions. Soluble RAGE (sRAGE) level is elevated in patients with acute respiratory distress syndrome (ARDS). However, which clinical parameters and inflammatory biomarkers including sRAGE are associated with death in ARDS pati...

journal_title：Clinical biochemistry

authors： Nakamura T,Sato E,Fujiwara N,Kawagoe Y,Maeda S,Yamagishi S

更新日期：2011-06-01 00:00:00

abstract：OBJECTIVE:Recently cortisol level in hair is supposed to be a good biomarker of chronic stress. We had developed a new method based on HPLC-FLU for identification of cortisol in human hair and measured cortisol distribution of each 1-cm segment along hair shaft. DESIGN AND METHODS:Hair sample was pulverized and incuba...

journal_title：Clinical biochemistry

authors： Gao W,Xie Q,Jin J,Qiao T,Wang H,Chen L,Deng H,Lu Z

更新日期：2010-05-01 00:00:00

abstract：OBJECTIVES:Newly presented solid-phase extraction methods (Solid Phase Disc Extraction, SPEC-Plus, Multi-Modal, and Solid Phase Micro Extraction, SPME have been checked with respect to their applicability to clinical toxicological analysis. In comparison with conventional liquid/liquid-extraction and a common mixed-pha...

journal_title：Clinical biochemistry

authors： Degel F

更新日期：1996-12-01 00:00:00

abstract：OBJECTIVE:Living kidney donors have been part of a successful kidney transplant programme in Norway for almost 50 years. Glomerular filtration rates (GFRs) have tended to remain stable at about 70% of pre-donation levels. Plasma total homocysteine (Hcy) has an inverse relationship to kidney function, and previous repor...

journal_title：Clinical biochemistry

authors： Apeland T,Holdaas H,Mansoor MA

更新日期：2014-04-01 00:00:00

abstract：OBJECTIVES:The use of iodinated contrast media has grown in popularity in the past two decades, but relatively little attention has been paid to the possible interferential effects of contrast media on laboratory test results. Herein, we investigate medical contrast media interference with routine chemistry results obt...

journal_title：Clinical biochemistry

authors： Park YJ,Rim JH,Yim J,Lee SG,Kim JH

更新日期：2017-08-01 00:00:00

abstract：BACKGROUND:While investigating estrogen response element (ERE) binding properties of human estrogen receptor-α (hERα) in breast cancer cytosols, other ERE-binding proteins (ERE-BP) were observed. DESIGN AND METHODS:Recognition properties of ERE-BP were evaluated by electrophoretic mobility shift assays (EMSA) with ERE...

journal_title：Clinical biochemistry

authors： Kruer TL,Cummins TD,Powell DW,Wittliff JL

更新日期：2013-11-01 00:00:00

abstract：BACKGROUND:The accurate early diagnosis of acute kidney injury (AKI) in patients with acute heart failure (AHF) is an unmet clinical need. Cystatin C might improve the early detection of AKI. METHODS:207 patients presenting to the emergency department with AHF were enrolled. Cystatin C was measured in plasma in a blin...

journal_title：Clinical biochemistry

authors： Breidthardt T,Sabti Z,Ziller R,Rassouli F,Twerenbold R,Kozhuharov N,Gayat E,Shrestha S,Barata S,Badertscher P,Boeddinghaus J,Nestelberger T,Mueller C

更新日期：2017-12-01 00:00:00

abstract：OBJECTIVES:The aims of the study were to test the susceptibility of THP-1 macrophages to develop oxidative stress and to deploy antioxidant defense mechanisms that insure the balance between the pro- and antioxidant molecules. DESIGN AND METHODS:Differentiated THP-1 were incubated in the presence or absence of iron-as...

journal_title：Clinical biochemistry

authors： Marcil V,Lavoie JC,Emonnot L,Seidman E,Levy E

更新日期：2011-07-01 00:00:00

abstract：OBJECTIVES:To ascertain the effects of eating on plasma antioxidant capacity in patients with liver disease. DESIGN AND METHODS:Eighteen cirrhotic patients were compared to 18 age and sex-matched controls. TRAP was measured by a fluorometric assay after a 12 h fast, and 60, 120, and 180 min after the study participant...

journal_title：Clinical biochemistry

authors： Pirisi M,Federico E,Bortolotti N,Fabris C,Falleti E,Soardo G,Gonano F,Bartoli E

更新日期：1999-08-01 00:00:00