Modifications to commercial oxalate oxidase based determination of urinary oxalate: a method suitable for routine clinical analysis.

Abstract:

:A commercially available assay (Sigma) for urinary oxalates based on the oxalate oxidase/peroxidase catalyzed reaction produced, in our hands, recoveries of added oxalate that varied from patient to patient. A modification of the assay is reported that involves the chromatographic separation of interfering substances, such as ascorbate and uric acid, from oxalate prior to analysis. With the modification recoveries of added oxalate were essentially complete and showed less patient-to-patient variability. Correlation with an alternate procedure was greatly improved.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Mazzuchin A,Michelutti L,Falter H

doi

10.1016/0009-9120(90)80032-e

subject

Has Abstract

pub_date

1990-04-01 00:00:00

pages

173-7

issue

2

eissn

0009-9120

issn

1873-2933

pii

0009-9120(90)80032-E

journal_volume

23

pub_type

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