A simpler diagnostic formula for screening nonalcoholic fatty liver disease.


OBJECTIVE:To increase the accuracy of non-invasive diagnosis of nonalcoholic fatty liver disease (NAFLD), clinical and laboratory NAFLD indicators were integrated into a diagnostic formula. METHODS:A total of 141 patients with clinically diagnosed NAFLD and 30 healthy controls were enrolled. We collected case history, body weight, height and mass index (BMI), alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyl transpeptidase, blood urea nitrogen and blood uric acid (UA), serum creatinine, plasma total cholesterol, triglyceride, low density lipoprotein, glycosylated hemoglobin, fasting plasma glucose, fasting insulin, ultrasonic tests, Fibroscans, and other data. Linear correlation, multiple linear regressions, and receiver operating characteristic (ROC) curve methods were used to process and analyze the collected data. The performance of Fibroscan and our diagnostic formula was compared in reference to the findings of liver biopsy. RESULTS:The identified NAFLD diagnostic indices consisted of BMI, ALT, AST and UA. A regression formula was proposed as: CAP = 113.163 + 0.252 * ALT + 6.316 * BMI. Diagnosis of the area under the ROC curve was 0.927, the sensitivity was 87.68%, and specificity was 90%. The cutoff was 277.67 (p < 0.01). The accuracy of the NAFLD diagnosis with the proposed formula was significantly higher than FibroScan (82.6% vs 69.6%; p = 0.005). CONCLUSIONS:NAFLD diagnosis with the proposed formula demonstrated both high sensitivity and specificity, and its accuracy was significantly higher than FibroScan. This formula only utilized non-invasive clinical and laboratory findings and the calculation was simple. It can be conveniently used for clinical diagnosis of NAFLD.


Clin Biochem


Clinical biochemistry


Feng G,He N,Zhou YF,Li XP,Niu C,Liu ML,Zhang KL,Li Y,Li YM,Zheng MH,Mi M




Has Abstract


2019-02-01 00:00:00












  • B-type natriuretic peptide in pediatrics.

    abstract:OBJECTIVES:There has been growing interest in the use of serum B-type natriuretic peptide (BNP) and the N-terminal segment of its pro-hormone (NT-proBNP) as biomarkers for cardiac disease. The aim of this review is to summarize the current state of knowledge regarding BNP and NT-proBNP measurement in the pediatric popu...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审


    authors: Davis GK,Bamforth F,Sarpal A,Dicke F,Rabi Y,Lyon ME

    更新日期:2006-06-01 00:00:00

  • Paraoxonase-1 activity is positively related to phospholipid transfer protein activity in type 2 diabetes mellitus: Role of large HDL particles.

    abstract:OBJECTIVES:High density lipoprotein (HDL)-associated paraoxonase-1 (PON-1) exerts anti-oxidative properties, whereas phospholipid transfer protein (PLTP) is able to convert mature HDL into larger and smaller HDL particles. Here we tested associations of PON-1 with PLTP in type 2 diabetes mellitus (T2DM), a condition ch...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Dullaart RPF,Gruppen EG,Dallinga-Thie GM

    更新日期:2016-04-01 00:00:00

  • Measurement of cystine in granulocytes using liquid chromatography-tandem mass spectrometry.

    abstract:BACKGROUND:Cystinosis is a rare autosomal recessive disorder characterized by an accumulation of intralysosomal cystine due to a defect in cystine transport across the lysosomal membrane. This disorder can be treated specifically using high doses of cysteamine. Accurate measurement of intracellular cystine content is n...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Chabli A,Aupetit J,Raehm M,Ricquier D,Chadefaux-Vekemans B

    更新日期:2007-06-01 00:00:00

  • Role of sex and time of blood sampling in SOD1 and SOD2 expression variability.

    abstract:OBJECTIVES:This study investigates how some of human physiological parameters, in particular sex and daytime, can contribute to the normal variation in blood gene expression. DESIGN AND METHODS:The expressions of two genes: SOD1 (Cu/Zn) and SOD2 (Mn) in white blood cells of 19 healthy individuals (10 men and 9 women) ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Khymenets O,Covas MI,Farré M,Langohr K,Fitó M,de la Torre R

    更新日期:2008-11-01 00:00:00

  • Association of glycated albumin to HbA1c ratio with diabetic retinopathy but not diabetic nephropathy in patients with type 2 diabetes.

    abstract:OBJECTIVES:The ratio of glycated albumin to HbA1c (GA/HbA1c ratio) is a known indicator that reflects fluctuations in plasma glucose. In this study, the association of the GA/HbA1c ratio to diabetic nephropathy and diabetic retinopathy in patients with type 2 diabetes was investigated. DESIGN AND METHODS:Among patient...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Umayahara Y,Fujita Y,Watanabe H,Kasai N,Fujiki N,Hatazaki M,Koga M

    更新日期:2017-04-01 00:00:00

  • Lateral flow assay for simultaneous detection of cellular- and humoral immune responses.

    abstract:OBJECTIVE:The development of a cytokine detection assay suitable for detection of multiple biomarkers for improved diagnosis of mycobacterial diseases. DESIGN AND METHODS:A lateral flow (LF) assay to detect IL-10 was developed utilizing the up-converting phosphor (UCP) reporter-technology. The assay was evaluated usin...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Corstjens PL,de Dood CJ,van der Ploeg-van Schip JJ,Wiesmeijer KC,Riuttamäki T,van Meijgaarden KE,Spencer JS,Tanke HJ,Ottenhoff TH,Geluk A

    更新日期:2011-10-01 00:00:00

  • Relationship between measured average glucose by continuous glucose monitor and HbA1c measured by three different routine laboratory methods.

    abstract:OBJECTIVES:The relationship between glycated hemoglobin A1c (HbA1c) and average glucose has been described by the empirically derived estimated average glucose (eAG) equation in the A1c-Derived Average Glucose (ADAG) study, with extensive calibration efforts in four secondary reference HbA1c methods. It is not known if...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Loh TP,Sethi SK,Wong MS,Tai ES,Kao SL

    更新日期:2015-05-01 00:00:00

  • Usefulness of a new dialysis device adapted to small volume of red blood cells and its interest in epidemiology.

    abstract:OBJECTIVES:We checked the efficiency of a new dialysis device adapted to small volumes to remove glycerol from cryopreserved red blood cells. DESIGN AND METHODS:Dialysis was performed on D-Tube96™ Dialyzer Mini device. In a preliminary trial, we measured the residual glycerol before, and 2, 4 and 24 h after dialysis. ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Zerimech F,Huyvaert H,Matran R,Nadif R

    更新日期:2011-06-01 00:00:00

  • Detection of glucosylsphingosine in dried blood spots for diagnosis of Gaucher disease by LC-MS/MS.

    abstract:INTRODUCTION:Gaucher disease (GD) is caused by a deficiency of β-glucosidase (GCase), leading to accumulation of glucosylceramide (GlcC) and glucosylsphingosine (Lyso-Gb1). Lyso-Gb1 is a reliable biomarker for GD. OBJECTIVES:This study aims to develop a simple, effective and accurate method for the screening and diagn...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Tang C,Jia X,Tang F,Liu S,Jiang X,Zhao X,Sheng H,Peng M,Liu L,Huang Y

    更新日期:2021-01-01 00:00:00

  • Interaction of chemicals with cytochrome P-450: implications for the porphyrinogenicity of drugs.

    abstract::Our studies lead us to suggest that in order for an organic chemical to cause porphyrin accumulation in chick embryo hepatocyte culture, it must be: 1. lipophilic; 2. resistant to biotransformation by phase I or phase II reactions to compounds which are devoid of biological activity; and 3. capable of interacting with...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Marks GS,McCluskey SA,Mackie JE,Riddick DS,James CA

    更新日期:1989-06-01 00:00:00

  • Cortisol and cortisone analysis in serum and plasma by atmospheric pressure photoionization tandem mass spectrometry.

    abstract:OBJECTIVES:Cortisol metabolism is controlled by 11beta-hydroxysteroid dehydrogenase (11beta-HSD) isoenzymes, which interconvert cortisol and cortisone. Accurate measurement of the cortisol and cortisone concentrations and their ratio provide useful information about 11beta-HSD activity. METHODS:Cortisol and cortisone ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Kushnir MM,Neilson R,Roberts WL,Rockwood AL

    更新日期:2004-05-01 00:00:00

  • Galactosemia: screening and diagnosis.

    abstract::Galactose is normally metabolized to glucose through the coordinated activities of three enzymes: galactokinase, galactose-1-phosphate uridyl transferase (GALT), and uridine diphospho-glucose 4-epimerase (epimerase). High concentrations of galactose and their metabolites are toxic to mammals. Hereditary deficiencies o...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审


    authors: Beutler E

    更新日期:1991-08-01 00:00:00

  • mRNA levels of enzymes and receptors implicated in arachidonic acid metabolism in gliomas.

    abstract:BACKGROUND:Gliomas are tumors of the central nervous system derived from glial cells. They show cellular heterogeneity and lack specific diagnostic markers. Although a possible role for the eicosanoid cascade has been suggested in glioma tumorigenesis, the relationship between enzymes and receptors implicated in arachi...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: De Armas R,Durand K,Guillaudeau A,Weinbreck N,Robert S,Moreau JJ,Caire F,Acosta G,Pebet M,Chaunavel A,Marin B,Labrousse F,Denizot Y

    更新日期:2010-07-01 00:00:00

  • Incremental value of intact fibroblast growth factor 23 to natriuretic peptides for long-term risk estimation of heart failure patients.

    abstract::Fibroblast growth factor 23 (FGF-23), a key hormone for the regulation of the phosphorus homeostasis, has also several direct effects on cardiac function. In heart failure (HF), the increase of FGF-23 participates to cardiac hypertrophy and remodeling. Measurement of the intact, biologically active hormone is now avai...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Gruson D,Carbone V,Feracin B,Ahn SA,Rousseau MF

    更新日期:2018-11-01 00:00:00

  • The TCN2 776CNG polymorphism correlates with vitamin B(12) cellular delivery in healthy adult populations.

    abstract:OBJECTIVES:Vitamin B(12), or B(12), is an essential nutrient for humans, and its deficiency is a public health problem, especially in elderly population. Around 30% of circulating total B(12) levels are attached to transcobalamin II (TCN2), being referred as holotranscobalamin (holo-TC), and representing the biological...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Castro R,Barroso M,Rocha M,Esse R,Ramos R,Ravasco P,Rivera I,de Almeida IT

    更新日期:2010-05-01 00:00:00

  • Elevated red blood cell distribution width at ICU discharge is associated with readmission to the intensive care unit.

    abstract:BACKGROUND:Red blood cell distribution width (RDW) is a predictor of mortality in critically ill patients. Our objective was to investigate the association between the RDW at ICU discharge and the risk of ICU readmission or unexpected death in the ward. METHODS:A secondary analysis of prospectively collected data stud...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Tonietto TA,Boniatti MM,Lisboa TC,Viana MV,Dos Santos MC,Lincho CS,Pellegrini JAS,Vidart J,Neyeloff JL,Faulhaber GAM

    更新日期:2018-05-01 00:00:00

  • Circulating miRNA as fine regulators of the physiological responses to physical activity: Pre-analytical warnings for a novel class of biomarkers.

    abstract::MicroRNAs are endogenous non-coding RNAs that post-transcriptionally regulate gene expression by specifically binding the target mRNA and by consequently inducing its degradation. miRNAs can be released into the circulation where they remain stable and they can be measured. Their changes reflect individual biologic ad...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审


    authors: Lombardi G,Perego S,Sansoni V,Banfi G

    更新日期:2016-12-01 00:00:00

  • Ischemic acute kidney injury and klotho in renal transplantation.

    abstract::Post-transplant ischemic acute kidney injury (AKI), secondary to ischemia reperfusion injury (IRI), is a major problem influencing on the short and long term graft and patient survival. Many molecular and cellular modifications are observed during IRI, for example, tissue damage result production of reactive oxygen sp...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审


    authors: Panah F,Ghorbanihaghjo A,Argani H,Asadi Zarmehri M,Nazari Soltan Ahmad S

    更新日期:2018-05-01 00:00:00

  • An audit of holotranscobalamin ("Active" B12) and methylmalonic acid assays for the assessment of vitamin B12 status: application in a mixed patient population.

    abstract:BACKGROUND:Vitamin B12 insufficiency/deficiency is common in mixed patient populations. However there is no single marker which can reliably diagnose B12 insufficiency/deficiency. Elevated concentrations of methylmalonic acid (MMA) are considered the most representative marker of metabolic vitamin B12 insufficiency, bu...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Sobczyńska-Malefora A,Gorska R,Pelisser M,Ruwona P,Witchlow B,Harrington DJ

    更新日期:2014-01-01 00:00:00

  • A reproducible estimation of triiodothyronine uptake.

    abstract::A simple method for the determination of triodothyronine (T3) uptake using I125-T3 and dextran coated charcoal is described. This reaction is time dependent after the addition of dextran coated charcoal. Effect of temperature, concentrations of I125-T3 and charcoal are reported. Within-run precision has been estimated...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Locong AH

    更新日期:1976-02-01 00:00:00

  • Effect of dehydroepiandrosterone (DHEA) treatment on oxidative energy metabolism in rat liver and brain mitochondria. A dose-response study.

    abstract:OBJECTIVES:Effects of treatment with dehydroepiandrosterone (DHEA) on oxidative energy metabolism in rat liver and brain mitochondria were examined. DESIGN AND METHODS:Young adult rats were administered DHEA (0.1, 0.2, 1.0 or 2.0 mg/kg body weight) by subcutaneous route for 7 consecutive days. RESULTS:DHEA treatment ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Patel MA,Katyare SS

    更新日期:2007-01-01 00:00:00

  • Pediatric reference values for serum zinc concentration in Iranian subjects and an assessment of their dietary zinc intakes.

    abstract:OBJECTIVES:To determine pediatric reference values for serum zinc concentration in Iranian subjects. DESIGN AND METHODS:Serum zinc concentration was measured by flame atomic absorption spectrometry in 699 children and adolescents. Reference values for serum zinc were determined according to the Clinical and Laboratory...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Ghasemi A,Zahediasl S,Hosseini-Esfahani F,Syedmoradi L,Azizi F

    更新日期:2012-10-01 00:00:00

  • Quantitative analysis of trace elements in human blood and plasma by energy dispersive X-ray fluorescence.

    abstract::A photon excitation, secondary target x-ray fluorescence system was used to measure concentrations of Cu, Zn, Br and Rb in whole blood and plasma of healthy adults. The samples preparation method and calculated limits of detection of the technique are reported. Correlation statistics for elemental concentrations have ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Stump IG,Carruthers J,D'Auria JM

    更新日期:1977-06-01 00:00:00

  • Urine flow cytometer quantification of leukocytes in samples containing a large proportion of lymphocytes.

    abstract:OBJECTIVE:We compared leukocyte counts obtained by cytometric analysis and Fuchs-Rosenthal (FR) chamber counting in different proportions of lymphocytes (Lym%) suspensions and cerebrospinal fluid (CSF). DESIGN AND METHODS:UF-100 (UF) was evaluated. For preparation of cell suspensions, gradient density centrifugation m...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Yamanishi H,Imai N,Ohmine T,Nishiyama M,Suehisa E,Kanakura Y,Iwatani Y

    更新日期:2006-08-01 00:00:00

  • Inverse correlation of ribosomal protein S27A and multifunctional protein YB-1 in hepatocellular carcinoma.

    abstract:OBJECTIVE:The detection of possible correlation between ribosomal protein RPS27A and multifunctional YB-1 expression in hepatocellular carcinoma (HCC). DESIGN AND METHODS:Tissue microarray slides containing totally 80 cores with 19 tissues of HCC, 1 tissue of hepatocholangiocarcinoma, 10 tissues of liver cirrhosis and...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Gunasekaran VP,Ganeshan M

    更新日期:2014-09-01 00:00:00

  • Effect of second and third generation oral contraceptives on C-reactive protein, lipids and apolipoproteins in young, non-obese, non-smoking apparently healthy women.

    abstract:OBJECTIVE:Third generation oral contraceptives (OC) may increase the risk of cardiovascular events in healthy young women. DESIGN AND METHODS:We assessed the effect of second and third generation OC on CRP, lipids and apolipoproteins in 128 women. RESULTS:CRP was significantly higher in third generation contraceptive...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Krintus M,Sypniewska G,Kuligowska-Prusinska M

    更新日期:2010-04-01 00:00:00

  • Antioxidative enzymes and increased oxidative stress in depressive women.

    abstract:OBJECTIVES:To investigate the activities of the main antioxidative enzymes and oxidative stress in women with depressive disorder (DD). METHODS:In 35 drug-naive women with DD and 35 age matched healthy women enzymes superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GPX1), glutathione reductase (G...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Kodydková J,Vávrová L,Zeman M,Jirák R,Macásek J,Stanková B,Tvrzická E,Zák A

    更新日期:2009-09-01 00:00:00

  • Screening for adenylosuccinate lyase deficiency using tandem mass spectrometry analysis of succinylpurines in neonatal dried blood spots.

    abstract:OBJECTIVES:Stable isotope dilution coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) is the sensitive method for screening for various inherited metabolic disorders using dried blood spots (DBSs). We present a method for LC-MS/MS determination of succinyladenosine (SAdo) and succinylaminoimidazole ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Zikanova M,Krijt J,Skopova V,Krijt M,Baresova V,Kmoch S

    更新日期:2015-01-01 00:00:00

  • Evaluation of predictive value of pleural CEA in patients with pleural effusions and histological findings: A prospective study and literature review.

    abstract:OBJECTIVES:Pleural effusion recognizes heterogeneous etiology and pathogenesis and requires invasive diagnostic procedures. Usually, after pleural fluid analysis, 30-50% of patients with malignant pleural effusion exhibit negative pleural cytology, and the sensitivity of image-guided pleural needle-aspiration biopsy ra...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审


    authors: Tozzoli R,Basso SM,D'Aurizio F,Metus P,Lumachi F

    更新日期:2016-11-01 00:00:00

  • Electrospray mass spectrometric characterization of hemoglobin Q (Hb Q-India) and a double mutant hemoglobin S/D in clinical samples.

    abstract:OBJECTIVES:The clinical analysis of hemoglobin by ion exchange chromatography can result in ambiguities in identification of the nature of the globin chain present in patient samples. LC/ESI-MS provides rapid and precise determination of globin chain masses. DESIGN AND METHODS:Hemolysate of hemoglobin Q-India and hemo...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Mandal AK,Bisht S,Bhat VS,Krishnaswamy PR,Balaram P

    更新日期:2008-01-01 00:00:00