Diagnostic implications of enumerating and reporting beta fraction(s) for the detection of beta-migrating monoclonal immunoglobulins in serum protein electrophoresis.

abstract：BACKGROUND:With the advent of the new high-sensitivity troponin assays, it is becoming critical to measure troponin accurately to low concentrations. To ensure assay performance is acceptable, appropriate QC must be run. METHODS:In addition to the routine use of commercial QC material, we prepared pools of human QC ma...

journal_title：Clinical biochemistry

authors： Hickman PE,Koerbin G,Badrick T,Oakman C,Potter JM

更新日期：2018-08-01 00:00:00

abstract：:We investigated the effects of medium supplementation with increasing amounts of different fatty acids on HepG2 cell cholesterol biosynthesis and esterification. Up to 200 microM 16:0 led to an increase in cholesterol secretion/synthesis of 60%/40%; 18:1, 18:2, or 18:3 decreased secretion/synthesis by 35%/25%, 65%/65%...

journal_title：Clinical biochemistry

authors： Schäfer HF,Kattermann R

更新日期：1992-10-01 00:00:00

abstract：OBJECTIVES:Carbonylation of the protein amino, guanidino and thiol groups is one of the important causes of vascular complications in diabetes. We developed a simple spectrophotometric method for monitoring of the changes in the protein amino group contents during carbonylation. DESIGN AND METHODS:The method is based ...

journal_title：Clinical biochemistry

authors： Aćimović JM,Jovanović VB,Veselinović MR,Srećković VD,Mandić LM

更新日期：2011-08-01 00:00:00

abstract：:The clinical role of HER-2/neu, a 185 kD epithelial transmembranous protein, has evolved after the approval of the anti-HER-2/neu targeted monoclonal antibody trastuzumab (Herceptin) for the therapy of metastatic breast cancer. The extracellular domain of HER-2/neu undergoes proteolytic cleavage from the full-length p...

journal_title：Clinical biochemistry

authors： Lüftner D,Lüke C,Possinger K

更新日期：2003-06-01 00:00:00

abstract：OBJECTIVE:To evaluate clinical value of oxidized lipoprotein(a) [ox-Lp(a)] levels. DESIGN AND METHODS:Ox-Lp(a) were measured by 2 ELISAs using antibodies against ox-Lp(a) [ox-Lp(a)1] or oxidized low-density lipoprotein [ox-Lp(a)2], and studied in 161 acute coronary syndromes (ACS) patients, 114 stable coronary artery ...

journal_title：Clinical biochemistry

authors： Wang JJ,Han AZ,Meng Y,Gong JB,Zhang CN,Li K,Liu YX

更新日期：2010-04-01 00:00:00

abstract：:An automated biotin-streptavidin procedure for measuring progesterone in serum is described. The method is linear up to 98.3 nmol/L and the calibration curve is stable for at least 14 days. The lower limit of progesterone detection is 0.70 nmol/L. Although serum is the preferred specimen, progesterone levels can be me...

journal_title：Clinical biochemistry

authors： Pesce MA,Bodourian SH,Sheehan M,Henkel CF

更新日期：1992-12-01 00:00:00

abstract：OBJECTIVES:All patients who undergo cardiopulmonary bypass (CPB) experience some degree of ischemia reperfusion injury (IRI). Severe IRI-induced acute kidney injury (AKI) occurs in approximately 1-2% of patients undergoing CPB. Previous studies using activity-based protein profiling of urine identified group XV phospho...

journal_title：Clinical biochemistry

authors： Navarrete M,Wilkins JA,Chan FV,Ye B,Nickerson P,Ho J

更新日期：2021-02-01 00:00:00

abstract：OBJECTIVES:Hepcidin is the main regulator of systemic iron homeostasis and its expression is modulated by iron status, hypoxia, erythroid factors and inflammation. The aim of our study was to examine a relationship between level of hepcidin and iron status, erythropoietic activity, hypoxia and inflammation in exacerbat...

journal_title：Clinical biochemistry

authors： Tandara L,Grubisic TZ,Ivan G,Jurisic Z,Tandara M,Gugo K,Mladinov S,Salamunic I

更新日期：2015-12-01 00:00:00

abstract：OBJECTIVE:To clarify the possibility that midband Lp in LDL fractions might act as an atherogenic lipoprotein in their interaction with macrophages. DESIGN AND METHODS:Low density lipoproteins (LDL) isolated by zonal ultracentrifugation from midband lipoprotein-positive serum in type lib hyperlipidemics were subjected...

journal_title：Clinical biochemistry

authors： Kawano M,Shinomiya M,Kanzaki T,Morisaki N,Shirai K,Saito Y,Yoshida S

更新日期：1996-06-01 00:00:00

abstract：OBJECTIVES:Regulatory T cells (Tregs) which may indicate operational tolerance provide a promising biomarker for individualization of immunosuppression. Naturally thymus-derived Tregs (nTregs) represent the major suppressive phenotype and can be identified by their demethylation status in the Tregs Specific Demethylate...

journal_title：Clinical biochemistry

authors： Metzker M,Shipkova M,von Ahsen N,Andag R,Abe M,Canzler O,Klett C,Leicht S,Olbricht C,Wieland E

更新日期：2016-10-01 00:00:00

abstract：OBJECTIVE:Recently cortisol level in hair is supposed to be a good biomarker of chronic stress. We had developed a new method based on HPLC-FLU for identification of cortisol in human hair and measured cortisol distribution of each 1-cm segment along hair shaft. DESIGN AND METHODS:Hair sample was pulverized and incuba...

journal_title：Clinical biochemistry

authors： Gao W,Xie Q,Jin J,Qiao T,Wang H,Chen L,Deng H,Lu Z

更新日期：2010-05-01 00:00:00

abstract：:New methods were developed for the separation of major lipid classes varying in polarity from cholesterol esters to lysophosphatidylcholine. The methods were used for the analysis of extracts obtained from human sera. The lipids were separated by overpressured thin-layer chromatography, classical thin-layer chromatogr...

journal_title：Clinical biochemistry

authors： Pucsok J,Kovács L,Zalka A,Dobó R

更新日期：1988-04-01 00:00:00

abstract：OBJECTIVES:We evaluated the effects of lifestyle modification (LM) on lipid profile, oxidative stress and serum-stimulated human coronary artery endothelial cell (HCAEC) viability in coronary artery disease (CAD) patients after 6months. DESIGN AND METHODS:Thirty patients with CAD were randomly assigned to LM intervent...

journal_title：Clinical biochemistry

authors： Srimahachota S,Wunsuwan R,Siritantikorn A,Boonla C,Chaiwongkarjohn S,Tosukhowong P

更新日期：2010-07-01 00:00:00

abstract：:Fucosidosis is an inherited lysosomal storage disease due to a deficiency of alpha-L-fucosidase activity. Exponentially growing lymphoid cell cultures from a fucosidosis patient (JH) had 16-fold lower extracellular alpha-L-fucosidase protein and 72-fold lower intracellular alpha-L-fucosidase protein with negligible ca...

journal_title：Clinical biochemistry

authors： DiCioccio RA,Gordon BA

更新日期：1991-06-01 00:00:00

abstract：CONTEXT:Knee arthroplasty surgery is significant trauma, leading to an activated immune system causing inflammation and oxidative stress. Many current biomarkers are invasive, costly, and often slow to analyse, limiting their use for rapid inflammatory measurements. OBJECTIVES:We have examined the use of urinary neopt...

journal_title：Clinical biochemistry

authors： Baxter-Parker G,Roffe L,Cross S,Frampton C,Hooper GJ,Gieseg SP

更新日期：2019-01-01 00:00:00

abstract：:Epidemiological evidence suggests that the ratio HDL-cholesterol (HDL-C)/total cholesterol (TC) or apolipoprotein A1 (apo A1)/apolipoprotein B (apo B) are good indicators of coronary heart disease risk. In investigating the distribution of these ratios in the typical population served by our routine laboratory, we ana...

journal_title：Clinical biochemistry

authors： Kuyl JM,Mendelsohn D

更新日期：1992-10-01 00:00:00

abstract：OBJECTIVES:To investigate the association between oxidative stress and certain trace elements in the blood of breast cancer patients. DESIGN AND METHODS:Malondialdehyde (MDA) was measured in serum of patients with breast cancer (n = 35) and controls (n = 35) by high performance liquid chromatography. Trace elements we...

journal_title：Clinical biochemistry

authors： Huang YL,Sheu JY,Lin TH

更新日期：1999-03-01 00:00:00

abstract：OBJECTIVE:Heat-mediated ELISA (HELISA) technique has been reported to shorten ELISA timings and has major clinical implications in diagnostic field [Bora U, Kannan K, Nahar P. Heat mediated enzyme linked immunosorbent assay. J Immunol Methods 2004; 293: 43-50]. Objective of this study is to find out whether anti-MBL au...

journal_title：Clinical biochemistry

authors： Sharma P,Gupta B,Farhat Basir S,Rani Das H,Nahar P

更新日期：2008-01-01 00:00:00

abstract：:The Laser Nephelometer PDQTM (Hyland Division, Travenol Laboratories Inc.) and the Abbott Bichromatic Analyser 100 (Abbott Laboratories) were compared with a radial immunodiffusion method. Seventy-eight serum specimens collected during routine blood testing were aliquoted and quantitated by the three procedures descri...

journal_title：Clinical biochemistry

authors： Bruver RM,Salkie ML

更新日期：1978-06-01 00:00:00

abstract：OBJECTIVES:Renal dysfunction has been reported in normoalbuminuric patients, demonstrating the necessity to improve the diagnostic and prognostic tools for diabetic kidney disease (DKD) investigation. Therefore, the aim of this study was to investigate whether the urinary levels of neutrophil gelatinase-associated lipo...

journal_title：Clinical biochemistry

authors： de Carvalho JA,Tatsch E,Hausen BS,Bollick YS,Moretto MB,Duarte T,Duarte MM,Londero SW,Premaor MO,Comim FV,Delanghe JR,Moresco RN

更新日期：2016-02-01 00:00:00

abstract：OBJECTIVES:High density lipoprotein (HDL)-associated paraoxonase-1 (PON-1) exerts anti-oxidative properties, whereas phospholipid transfer protein (PLTP) is able to convert mature HDL into larger and smaller HDL particles. Here we tested associations of PON-1 with PLTP in type 2 diabetes mellitus (T2DM), a condition ch...

journal_title：Clinical biochemistry

authors： Dullaart RPF,Gruppen EG,Dallinga-Thie GM

更新日期：2016-04-01 00:00:00

abstract：OBJECTIVES:Define performance characteristics of the Stratus CS cardiac troponin I (cTnI) assay in routine practice. METHODS:Imprecision at low cTnI values was assessed according to NCCLS EP5-A. Linearity was assessed by regression analysis; the reference interval was determined in 345 healthy individuals. Minimum det...

journal_title：Clinical biochemistry

authors： Christenson RH,Cervelli DR,Bauer RS,Gordon M

更新日期：2004-08-01 00:00:00

abstract：OBJECTIVES:Living donor kidney transplantation is regarded as beneficial to allograft recipients and not particularly detrimental to the donors. Recently we have documented a reduced glomerular filtration rate (GFR) in living kidney donors (LKDs). Considering kidneys as essential for l-arginine (Arg) metabolism, in the...

journal_title：Clinical biochemistry

authors： Zunić G,Tomić A,Spasić S

更新日期：2013-10-01 00:00:00

abstract：:We report a sensitive and specific method for the determination of AY-25,712 concentrations in rat, dog and human serum by liquid chromatography with UV detection. The detection limit is 25 ng/mL, based on 2 mL of serum or plasma. The method has been validated in human volunteers receiving single oral doses of AY-25,7...

journal_title：Clinical biochemistry

authors： Gonzalez R,Thibeault D

更新日期：1983-08-01 00:00:00

abstract：OBJECTIVES:During the development of a testosterone assay by LC-MS/MS, we encountered significant assay interference introduced by blood collection tubes. We examined a number of commonly used blood collection tubes for the presence of interference and its impact on testosterone quantitation. DESIGN AND METHODS:A numb...

journal_title：Clinical biochemistry

authors： Shi RZ,van Rossum HH,Bowen RA

更新日期：2012-12-01 00:00:00

abstract：BACKGROUND:Disease-independent sources of biomarker variability include pre-analytical, analytical and biological variance. The aim of the present study was to evaluate whether the pre-analytical phase has any impact on the emerging heart disease TWEAK and HMGB1 protein markers and miRNA biomarkers, and whether peptido...

journal_title：Clinical biochemistry

authors： Basso D,Padoan A,Laufer T,Aneloni V,Moz S,Schroers H,Pelloso M,Saiz A,Krapp M,Fogar P,Cornoldi P,Zambon CF,Rossi E,La Malfa M,Marotti A,Brefort T,Weis TM,Katus HA,Plebani M

更新日期：2017-03-01 00:00:00

abstract：OBJECTIVES:Analysis of serum/plasma methylmalonic acid (MMA) is important for the diagnosis and management of methylmalonic acidemia in pediatric populations. This work focuses on developing and validating a liquid chromatography tandem mass spectrometry (LC-MS/MS) method to monitor methylmalonic acidemia using a simpl...

journal_title：Clinical biochemistry

authors： Lo SY,Gordon C,Sadilkova K,Jack RM,Dickerson JA

更新日期：2016-09-01 00:00:00

abstract：:Three commercially available assays (an enzyme-linked immunosorbent assay ELISA, an immunoradiometric assay, IRMA, and a nephelometric assay) for the determination of lipoprotein(a) [Lp(a)] were compared with respect to the dependency of these assays on the various apolipoprotein(a) [apo(a)] isoforms. Although there w...

journal_title：Clinical biochemistry

authors： Leus FR,Leerink CB,Prins J,van Rijn HJ

更新日期：1994-12-01 00:00:00

abstract：:During the past decade, tandem mass spectrometry hyphenated to liquid chromatography separation systems (HPLC-MS/MS) has developed to an important technology in clinical chemistry - not only for research purposes but also for routine use. At present, most important application fields are target analyses in therapeutic...

journal_title：Clinical biochemistry

authors： Vogeser M,Seger C

更新日期：2008-06-01 00:00:00

abstract：OBJECTIVES:Microarray analysis of gene expression profiles of blood leukocytes has many potential clinical and research applications. DESIGN AND METHODS:We used the PAXgene Blood RNA System to prepare RNA from the whole blood of normal volunteers using two incubation times followed by gene expression profiling using t...

journal_title：Clinical biochemistry

authors： Wang J,Robinson JF,Khan HM,Carter DE,McKinney J,Miskie BA,Hegele RA

更新日期：2004-09-01 00:00:00