Optimizing RNA extraction yield from whole blood for microarray gene expression analysis.

abstract：:In order to provide quality control serum in the hypoglycemic range, pooled human serum was treated with yeast. Yeast destroyed about 50% of the serum glucose in about 4 1/2 hrs. The yeast-treated serum remained suitable for quality control of the most commonly analyzed clinical chemistry constituents which showed onl...

journal_title：Clinical biochemistry

authors： Pelletier O,Pryce FH,Logan JE

更新日期：1980-02-01 00:00:00

abstract：BACKGROUND:Increased levels of circulating endostatin predicts cardiovascular morbidity and impaired kidney function in the general population. The utility of endostatin as a risk marker for mortality in the emergency department (ED) has not been reported. AIM:Our main aim was to study the association between plasma e...

journal_title：Clinical biochemistry

authors： Carlsson AC,Wessman T,Larsson A,Leijonberg G,Tofik R,Ärnlöv J,Melander O,Ruge T

更新日期：2020-01-01 00:00:00

abstract：OBJECTIVES:Does abnormal betaine excretion persist? DESIGN AND METHODS:Patients (10) with abnormal excretion in 1998 were recalled in 2005. Subsequent urine samples were collected on 4 days from persistently abnormal subjects. RESULTS:Half the 1998 abnormal patients were abnormal in 2005. Only 1/20 controls was abnor...

journal_title：Clinical biochemistry

authors： Lever M,Atkinson W,George PM,Chambers ST

更新日期：2007-07-01 00:00:00

abstract：OBJECTIVES:Most clinical laboratories replaced their manual precipitation techniques for the determination of urinary protein with automated dye binding assays or benzethonium chloride-turbidimetric assays. Few studies have validated these assays for the measurement of urinary proteins in the normal range. DESIGN AND ...

journal_title：Clinical biochemistry

authors： Dube J,Girouard J,Leclerc P,Douville P

更新日期：2005-05-01 00:00:00

abstract：OBJECTIVES:To have a reliable diagnostic test, the influence of urine pH on the determination of the total activity of N-acetyl-beta-D-glucosaminidase (NAG) and NAG isoenzyme activities was studied. DESIGN AND METHODS:After ultrafiltration and dialysis of the acidic and alkaline urines, the B, A, and A2 forms of NAG w...

journal_title：Clinical biochemistry

authors： Mandić LM,Aćimović JM,Jovanović VB

更新日期：2005-04-01 00:00:00

abstract：UNLABELLED:Frozen biospecimens are crucial for translational research and contain well-preserved nucleic acids and protein. However, the risks of freezer failure as well as space, cost, and environmental concerns of frozen biospecimens are substantial. OBJECTIVE:The purpose of the study was to review the current statu...

journal_title：Clinical biochemistry

authors： Lou JJ,Mirsadraei L,Sanchez DE,Wilson RW,Shabihkhani M,Lucey GM,Wei B,Singer EJ,Mareninov S,Yong WH

更新日期：2014-03-01 00:00:00

abstract：OBJECTIVES:: To test if circulating forms of the soluble urokinase-type plasminogen activator receptor (suPAR) are potential biomarkers of plaque vulnerability. DESIGN AND METHODS:: Plasma concentrations of suPAR(I-III), suPAR(II-III) and uPAR(I) were measured by time-resolved fluorescence immunoassays in Caucasian pa...

journal_title：Clinical biochemistry

authors： Olson FJ,Thurison T,Ryndel M,Høyer-Hansen G,Fagerberg B

更新日期：2010-01-01 00:00:00

abstract：INTRODUCTION:Barricor™ Vacutainers® are a novel non-gel separator blood collection tube. These tubes enable faster pre-analytical processing which could reduce turnaround time and be beneficial in an acute care setting. We sought to evaluate the bias, stability, and integrity of plasma generated from these tubes compar...

journal_title：Clinical biochemistry

authors： Fournier JE,Northrup V,Clark C,Fraser J,Howlett M,Atkinson P,Shea JL

更新日期：2018-08-01 00:00:00

abstract：:57Co-cyanocobalamin is substituted for iodine labeled antigen in three commercially available solid phase radioimmunoassay kits to evaluate the extent of non-specific binding by determination of the supernatant and residual fraction of unbound ligand and thus obtain a value for corrected binding throughout the range o...

journal_title：Clinical biochemistry

authors： Carter P

更新日期：1978-06-01 00:00:00

abstract：BACKGROUND:Laboratory confirmation of alpha-1-antitrypsin (A1AT) deficiency may be achieved by multiple methods. Here, we compare the relative comprehensiveness and efficiency of pathogenic variant (PV) detection of four different protocols utilized at different diagnostic centres in Canada. METHODS:Diagnostic results...

journal_title：Clinical biochemistry

authors： Mattman A,Gilfix BM,Chen SX,DeMarco ML,Kyle BD,Parker ML,Agbor TA,Jung B,Selvarajah S,Barakauskas VE,Vaags AK,Estey MP,Nelson TN,Speevak MD

更新日期：2020-07-01 00:00:00

abstract：INTRODUCTION:Gaucher disease (GD) is caused by a deficiency of β-glucosidase (GCase), leading to accumulation of glucosylceramide (GlcC) and glucosylsphingosine (Lyso-Gb1). Lyso-Gb1 is a reliable biomarker for GD. OBJECTIVES:This study aims to develop a simple, effective and accurate method for the screening and diagn...

journal_title：Clinical biochemistry

authors： Tang C,Jia X,Tang F,Liu S,Jiang X,Zhao X,Sheng H,Peng M,Liu L,Huang Y

更新日期：2021-01-01 00:00:00

abstract：OBJECTIVE:The aim of our research is to determine whether the time of blood sampling and fasting of patients have an impact on TSH values. DESIGN AND METHODS:A total of 198 participants were enrolled in this study and classified into five groups: A--the first sample collection for TSH measurement was taken between 7:0...

journal_title：Clinical biochemistry

authors： Mirjanic-Azaric B,Stojakovic-Jelisavac T,Vukovic B,Stojanovic D,Vujnic M,Uletilovic S

更新日期：2015-12-01 00:00:00

abstract：OBJECTIVES:The haphazard distribution of fibrous tissue can interfere with quantitative methods for evaluating hepatic fibrosis. Inter-sample variation may represent a crucial issue when hydroxyproline measurement is used to quantify fibrosis. A comparative study of the hydroxyproline levels in normal and fibrotic rats...

journal_title：Clinical biochemistry

authors： Gomes AT,Bastos CG,Afonso CL,Medrado BF,Andrade ZA

更新日期：2006-12-01 00:00:00

abstract：:A fluorometric procedure based on the Bio-Rad Vanillylmandelic Acid (VMA) by High Performance Liquid Chromatography (HPLC) Test was developed. Detection by fluorescence provides better sensitivity and specificity than the Pisano spectrophotometric method. Use of a C18 mini-column extraction to extract VMA prior to HPL...

journal_title：Clinical biochemistry

authors： Huth JA,Bottjen PL,Ludvigsen CW

更新日期：1986-08-01 00:00:00

abstract：:We assayed serum levels of certain enzymes and tumor markers in patients after transcatheter arterial embolization (TAE) to evaluate the effectiveness of this treatment. Twenty patients had hepatocellular carcinoma and two patients had metastases to the liver from colon cancer. Assays were first done immediately after...

journal_title：Clinical biochemistry

authors： Yoshikawa C,Shimojo N,Naka K,Akai T,Okuda K,Kaminou T,Yamada T,Nakatsuka H

更新日期：1987-12-01 00:00:00

abstract：OBJECTIVE:To evaluate the potential diagnostic value of apolipoprotein E (ApoE) measurements in pediatric patients with invasive bacterial infections. DESIGN AND METHOD:A total of 185 pediatric patients were enrolled in this study, including 94 patients with confirmable infections and 91 patients without confirmable i...

journal_title：Clinical biochemistry

authors： Wang C,Wang Y,Wang A,Fu P,Yang Y

更新日期：2012-02-01 00:00:00

abstract：OBJECTIVES:To compare the circulating levels of cathepsin D in preeclamptic and normotensive pregnancies. DESIGN AND METHODS:Fifty pregnant and 20 healthy non-pregnant patients were enrolled in this study. Of the 50 pregnant patients, 15 were preeclamptic and 35 patients were normotensive. Serum levels of soluble cath...

journal_title：Clinical biochemistry

authors： Kim HY,Lee M,Kang HW,Moon C

更新日期：2013-12-01 00:00:00

abstract：OBJECTIVE:To identify independent predictors of serum butyrylcholinesterase (BuChE) activity and examine its relationship with the metabolic syndrome. DESIGN AND METHODS:Serum BuChE activity was measured and metabolic syndrome risk factors were assessed in 1097 healthy subjects recruited from the Newfoundland adult po...

journal_title：Clinical biochemistry

authors： Randell EW,Mathews MS,Zhang H,Seraj JS,Sun G

更新日期：2005-09-01 00:00:00

abstract：OBJECTIVE:To evaluate a novel surface plasmon resonance (SPR) system for the direct measurement of tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva. DESIGN AND METHODS:We measured the presence of the tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva using 2 different surface plasmon resona...

journal_title：Clinical biochemistry

authors： Liang YH,Chang CC,Chen CC,Chu-Su Y,Lin CW

更新日期：2012-12-01 00:00:00

abstract：OBJECTIVE:Erythrocyte pyrimidine 5'-nucleotidase (Pyr-5'-N) is highly sensitive to heavy metal inactivation in vitro and in vivo, and a number of studies have verified its usefulness as a biomarker of acute and chronic lead exposures. Retrospective and prospective studies attempted to determine whether the known linear...

journal_title：Clinical biochemistry

authors： Paglia DE,Renner SW,Bhambhani K

更新日期：1999-04-01 00:00:00

abstract：:Cytosolic proteins that specifically bind cyclosporine A and FK-506 were isolated and purified from the JURKAT human T-helper cell line. These binding proteins were purified by affinity, molecular weight exclusion and weak cation exchange column chromatography. Radiolabeled cyclosporine A specifically bound to a appro...

journal_title：Clinical biochemistry

authors： Palaszynski EW,Donnelly JG,Soldin SJ

更新日期：1991-02-01 00:00:00

abstract：:Proteomics, the analysis of expressed proteins, has been an important developing area of research for the past two decades [Anderson, NG, Anderson, NL. Twenty years of two-dimensional electrophoresis: past, present and future. Electrophoresis 1996;17:443-453]. Advances in technology have led to a rapid increase in app...

journal_title：Clinical biochemistry

authors： Aldred S,Grant MM,Griffiths HR

更新日期：2004-11-01 00:00:00

abstract：OBJECTIVES:Salivary analysis is a noninvasive alternative that may be more acceptable to patients, especially children. As such, it has the potential for incorporation into comprehensive, dynamic investigations of metabolic dysfunctions - a significant advancement over a single time point serum analysis. In this study,...

journal_title：Clinical biochemistry

authors： Chiu SK,Collier CP,Clark AF,Wynn-Edwards KE

更新日期：2003-05-01 00:00:00

abstract：OBJECTIVE:We seek to identify the differentially expressed miRNAs in the clear cell subtype (ccRCC) of kidney cancer. DESIGN AND METHODS:We performed a miRNA microarray analysis to compare the miRNA expression levels between ccRCC tissues and their normal counterpart. The top dysregulated miRNAs were validated by quan...

journal_title：Clinical biochemistry

authors： Chow TF,Youssef YM,Lianidou E,Romaschin AD,Honey RJ,Stewart R,Pace KT,Yousef GM

更新日期：2010-01-01 00:00:00

abstract：:An early diagnosis is crucial for effective triage and management of patients with suspected acute myocardial infarction (AMI). Although troponin testing is the cornerstone of diagnosis, the sensitivity of this biomarker is still suboptimal at patient admission. The heart-type fatty acid binding protein (H-FABP) is an...

journal_title：Clinical biochemistry

authors： Lippi G,Mattiuzzi C,Cervellin G

更新日期：2013-01-01 00:00:00

abstract：OBJECTIVE:To clarify the possibility that midband Lp in LDL fractions might act as an atherogenic lipoprotein in their interaction with macrophages. DESIGN AND METHODS:Low density lipoproteins (LDL) isolated by zonal ultracentrifugation from midband lipoprotein-positive serum in type lib hyperlipidemics were subjected...

journal_title：Clinical biochemistry

authors： Kawano M,Shinomiya M,Kanzaki T,Morisaki N,Shirai K,Saito Y,Yoshida S

更新日期：1996-06-01 00:00:00

abstract：BACKGROUND:High levels of intact and cleaved forms of the urokinase-type plasminogen activator receptor (uPAR) in both tissue and blood are associated with poor survival in several cancer diseases. The prognostic significance of uPAR in cholangiocarcinoma is unknown. The aims of this study were to determine if pre-trea...

journal_title：Clinical biochemistry

authors： Grunnet M,Christensen IJ,Lassen U,Jensen LH,Lydolph M,Lund IK,Thurison T,Høyer-Hansen G,Mau-Sørensen M

更新日期：2014-05-01 00:00:00

abstract：OBJECTIVES:Measurements of myoglobin and creatine kinase (CK)-MB isoforms have been suggested to be sensitive tests for the early diagnosis of myocardial infarction (MI). We have investigated the utility of myoglobin, creatine kinase (CK)-MB isoforms and creatine kinase MB mass (CK-MBm) in early diagnosis of MI using c...

journal_title：Clinical biochemistry

authors： Penttilä K,Koukkunen H,Halinen M,Rantanen T,Pyörälä K,Punnonen K,Penttilä I

更新日期：2002-11-01 00:00:00

abstract：BACKGROUND:Patients suffering from hemorrhagic fever with renal syndrome (HFRS) often showed strikingly reduced high-density lipoprotein (HDL)-cholesterol levels during the oliguric phase, indicating severe alterations in lipoprotein metabolism. OBJECTIVE:To compare changes in the functions and composition of HDL, lip...

journal_title：Clinical biochemistry

authors： Cho KH,Park SH,Park JE,Kim YO,Choi I,Kim JJ,Kim JR

更新日期：2008-01-01 00:00:00

abstract：OBJECTIVES:The aims of the study were to test the susceptibility of THP-1 macrophages to develop oxidative stress and to deploy antioxidant defense mechanisms that insure the balance between the pro- and antioxidant molecules. DESIGN AND METHODS:Differentiated THP-1 were incubated in the presence or absence of iron-as...

journal_title：Clinical biochemistry

authors： Marcil V,Lavoie JC,Emonnot L,Seidman E,Levy E

更新日期：2011-07-01 00:00:00