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A convenient method for the determination of non-specific binding in commercially available solid phase iodine labeled radioimmunoassay kits.

Abstract：

:57Co-cyanocobalamin is substituted for iodine labeled antigen in three commercially available solid phase radioimmunoassay kits to evaluate the extent of non-specific binding by determination of the supernatant and residual fraction of unbound ligand and thus obtain a value for corrected binding throughout the range of the calibrated standard curve. In the present state of technology correction appears to have a trifling effect on the overall accuracy of the assay; however, the method as described allows the laboratorian to confirm for himself when testing a new product.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Carter P

doi

10.1016/s0009-9120(78)90070-x

subject

Has Abstract

pub_date

1978-06-01 00:00:00

pages

97-100

issue

3

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(78)90070-X

journal_volume

11

pub_type

杂志文章

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