Is laboratory medicine a dying profession? Blessed are those who have not seen and yet have believed.

Abstract:

:Technological developments and economic drivers have led to major changes in clinical laboratories worldwide, principally represented by an increase in testing productivity and efficiency. Currently, major expectations are entrusted on the introduction of diagnostically more effective tests as well as on translating the "omics" techniques into the clinical practice. However, the increased automation and standardization have far outpaced the laboratory professional involvement in assuring optimal test utilization and interpretation. While productivity of 'commodity' testing increases, the value of laboratory services seems to decrease contextually. This development represents a serious threat for laboratory medicine as an academic discipline not only because the laboratory professionals may lose the direct control of the commodity testing production activities but also because the present evolution exposes a failure of our core clinical activities, that is and remains the translation of laboratory data into valuable information for improving patient's outcomes. At a time when a revolution in healthcare organization is virtually unavoidable in the years ahead, clinical laboratories must proceed from a merely reactive strategy to a more proactive role in patient care. As patient-centered care is increasingly recognized as a primary focus in reorganizing the delivery of healthcare services, integrated practice units organized around the patients' needs and medical conditions are needed. An effective strategy to overcome the current inefficiencies in the delivery of laboratory services should encompass translation into integrated, multidisciplinary care, in which appropriateness in test request and interpretation both play a central role in assuring efficiency and effectiveness.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Plebani M,Lippi G

doi

10.1016/j.clinbiochem.2010.05.015

subject

Has Abstract

pub_date

2010-08-01 00:00:00

pages

939-41

issue

12

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(10)00249-3

journal_volume

43

pub_type

社论
  • Multicenter assessment of a hemoglobin A1c point-of-care device for diagnosis of diabetes mellitus.

    abstract:OBJECTIVE:A multisite investigation compared the analytical performance of a point-of-care (POC) HbA1c device with multiple commonly used HbA1c laboratory methods and an NGSP (National Glycohemoglobin Standardization Program) reference method. RESEARCH DESIGN AND METHODS:The Afinion AS100 POC device analyzed HbA1c usi...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,多中心研究

    doi:10.1016/j.clinbiochem.2018.09.007

    authors: Sobolesky PM,Smith BE,Saenger AK,Schulz K,Apple FS,Scott MG,Wu AHB,Little RR,Fitzgerald RL

    更新日期:2018-11-01 00:00:00

  • Sample application position and the resolution of protein separation on the Beckman Microzone Electrophoretic System.

    abstract::Many clinical laboratories use the Beckman Microzone electrophoretic system (Beckman Instruments Inc. Fullerton, CA 92634) for serum protein separation. Although detailed instruction is supplied in the manufacturer's manual (1) for protein separation, the effect of different sample application positions on the cellulo...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(78)80019-8

    authors: Chu SY,Turkington VE

    更新日期:1978-12-01 00:00:00

  • 1,5-anhydroglucitol monitoring in diabetes: a mass balance perspective.

    abstract::1,5-anhydroglucitol (AG) is a nonmetabolizable glucose analogue found in plasma due to ingestion. The normal steady-state concentration can be dramatically decreased by inhibition of tubular reabsorption during periods of hyperglycemia. For this reason, monitoring of AG has been plausibly advocated for detection of pe...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/j.clinbiochem.2008.08.086

    authors: Nerby CL,Stickle DF

    更新日期:2009-02-01 00:00:00

  • Detection of BRAF-V600E and V600K in melanoma circulating tumour cells by droplet digital PCR.

    abstract:OBJECTIVES:Defining the BRAF mutation status in metastatic melanoma patients is critical to selecting patients for therapeutic treatment with targeted therapies. Circulating tumour cells (CTCs) can provide an alternative source of contemporaneous tumour genetic material. However methodologies to analyse the presence of...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2014.12.007

    authors: Reid AL,Freeman JB,Millward M,Ziman M,Gray ES

    更新日期:2015-10-01 00:00:00

  • Determination of drugs of abuse in body fluids by radioimmunoassay.

    abstract::Only recently has radioimmunoassay been used for the detection of drugs of abuse in body fluids. The radioimmunoassay method is rapid, sensitive, specific, and can be performed with a minimum of sample, while conventional assay methods are time-consuming, relatively insensitive, and require a larger sample volume. Per...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(78)90098-x

    authors: Castro A,Mittleman R

    更新日期:1978-06-01 00:00:00

  • Solid phase measurements of antibody and lectin binding to xenogenic carbohydrate antigens.

    abstract:OBJECTIVES:In future pig-to-man xenotransplantation it is important to master tools that identify potentially xenogenic alphagalactose (Galalpha) antigens in the doner tissue. DESIGN AND METHODS:We have measured the binding potentials of Galalpha detecting lectins and antibodies, including a naturally occurring subfra...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(03)00120-6

    authors: Kirkeby S,André S,Gabius HJ

    更新日期:2004-01-01 00:00:00

  • Technological trends in clinical laboratory science.

    abstract:OBJECTIVE:This article will review the advancements and new developments being made in (1) advanced computers, (2) microtechnology, (3) advanced immunodiagnostics, (4) neural networks, and (5) molecular biology. The influence of these technologies and their products on clinical laboratories is also discussed. CONCLUSI...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/0009-9120(94)00075-7

    authors: Burtis CA

    更新日期:1995-06-01 00:00:00

  • Interindividual variation in the partition of lipoprotein(a) into lipoprotein subfractions.

    abstract::In order to evaluate cardiovascular risk, we analyzed the lipid composition of HDL and the presence of lipoprotein(a) [Lp(a)] by both agarose gel electrophoresis and enzyme-linked immunoassay (ELISA). In 681 plasmas we found a close correspondence between the existence of a visible sinking pre-beta lipoprotein band an...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/0009-9120(93)90117-o

    authors: Alvarez JJ,Lasunción MA,Olmos JM,Herrera E

    更新日期:1993-10-01 00:00:00

  • Immunoassays with time-resolved fluorescence spectroscopy: principles and applications.

    abstract::This review deals with the application of time-resolved fluorescence spectroscopy in the field of immunoassay. The advantages of time-resolved fluorescence over conventional fluorometry are discussed and it is explained why the fluorescent lanthanide chelates, and especially the Eu3+ chelates, are the labels of choice...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/0009-9120(88)90001-x

    authors: Diamandis EP

    更新日期:1988-06-01 00:00:00

  • Rapid and quantitative detection of CpG-methylation status using TaqMan PCR combined with methyl-binding-domain polypeptide.

    abstract:OBJECTIVES:To assess the medical applicability of CpG methylation as molecular markers for cancer diagnosis, we established a new system to determine DNA methylation based on TaqMan PCR combined with a methyl-binding-domain polypeptide 2. DESIGN AND METHODS:We evaluated the diagnostic applicability of this approach by...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2009.03.017

    authors: Kimura N,Moribe T,Iizuka N,Miura T,Tamatsukuri S,Ishitsuka H,Hamamoto Y,Oka M

    更新日期:2009-07-01 00:00:00

  • Doubling immunochemistry laboratory testing efficiency with the cobas e 801 module while maintaining consistency in analytical performance.

    abstract:OBJECTIVES:The new immunochemistry cobas e 801 module (Roche Diagnostics) was developed to meet increasing demands on routine laboratories to further improve testing efficiency, while maintaining high quality and reliable data. DESIGN AND METHODS:During a non-interventional multicenter evaluation study, the overall pe...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,多中心研究

    doi:10.1016/j.clinbiochem.2018.05.019

    authors: Findeisen P,Zahn I,Fiedler GM,Leichtle AB,Wang S,Soria G,Johnson P,Henzell J,Hegel JK,Bendavid C,Collet N,McGovern M,Klopprogge K

    更新日期:2018-08-01 00:00:00

  • Recent advances of liquid chromatography-(tandem) mass spectrometry in clinical and forensic toxicology.

    abstract::Liquid chromatography (LC) coupled to mass spectrometry (MS) or tandem mass spectrometry (MS/MS) has become increasingly important in clinical and forensic toxicology as well as doping control and is now a robust and reliable technique for routine analysis in these fields. In recent years, methods for LC-MS(/MS)-based...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/j.clinbiochem.2010.08.008

    authors: Peters FT

    更新日期:2011-01-01 00:00:00

  • Development of an immunoassay for differentiating human immunodeficiency virus infections--from vaccine-induced immune response in Tiantan vaccine trials in China.

    abstract:OBJECTIVE:To develop a Rapid Flow-through assay for distinguishing replicating Tiantan vaccine-generated serological response from true HIV infection. DESIGN AND METHOD:A Rapid Flow-through Test including gp41, gp36, sk1, sk2 and sk3 antigens was established and the performance of the assay was evaluated in clinical s...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2012.05.013

    authors: Yuan Z,Chen W,Zhang J,Zhang J,Xiang T,Hu J,Wu Z,Du X,Huang A,Zheng J

    更新日期:2012-10-01 00:00:00

  • 5' CpG island methylation analysis identifies the MAGE-A1 and MAGE-A3 genes as potential markers of HCC.

    abstract:OBJECTIVES:The change in DNA methylation patterns can be used to distinguish between normal and cancer cells. The aim of the present study was to examine the 5' CpG island methylation patterns of the cancer-testis antigen (CT antigen) gene family, MAGE-As, in hepatocellular carcinoma (HCC), and to develop the DNA demet...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2006.01.014

    authors: Qiu G,Fang J,He Y

    更新日期:2006-03-01 00:00:00

  • Hyaluronic acid as a non-invasive biomarker of liver fibrosis.

    abstract:UNLABELLED:Chronic liver diseases may cause inflammation and progressive scarring, over time leading to irreversible hepatic damage (cirrhosis). As a result, the need to assess and closely monitor individuals for risk factors of components of matrix deposition and degradation, as well as the severity of the fibrosis us...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/j.clinbiochem.2015.07.019

    authors: Neuman MG,Cohen LB,Nanau RM

    更新日期:2016-02-01 00:00:00

  • Comparison of mycophenolic acid concentrations determined by a new PETINIA assay on the dimension EXL analyzer and a HPLC-UV method.

    abstract:OBJECTIVE:Mycophenolic acid requires routine therapeutic drug monitoring. We evaluated the suitability of a new PETINIA (particle enhanced turbidimetric inhibition immunoassay) assay on the Dimension EXL analyzer for monitoring of mycophenolic acid by comparing values obtained by this assay with a HPLC-UV method. DESI...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2012.11.025

    authors: Dasgupta A,Tso G,Chow L

    更新日期:2013-05-01 00:00:00

  • Proportion of conjugated bilirubin in bile in relation to hepatic bilirubin UDP-glucuronyltransferase activity.

    abstract::To elucidate the diagnostic relevance of biliary conjugated bilirubin, biliary bilirubin from normal volunteers (NV), patients with Gilbert's syndrome (GS) and Crigler-Najjar syndrome type II (C-N II), and from various rat strains was fractionated. Biliary bilirubin diglucuronide (BDG) was present at lower levels, and...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/0009-9120(90)80024-d

    authors: Adachi Y,Yamashita M,Nanno T,Yamamoto T

    更新日期:1990-04-01 00:00:00

  • Do platelet apoptosis, activation, aggregation, lipid peroxidation and platelet-leukocyte aggregate formation occur simultaneously in hyperlipidemia?

    abstract:OBJECTIVES:The circulating lipoproteins may cause some abnormalities in platelet composition and function in hypercholesterolemia. The aim of this study was to investigate whether platelet apoptosis, platelet activation, platelet aggregation, platelet-leukocyte aggregate (PLA) formation and lipid peroxidation occur sim...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2005.09.005

    authors: Sener A,Ozsavci D,Oba R,Demirel GY,Uras F,Yardimci KT

    更新日期:2005-12-01 00:00:00

  • Myoglobin, creatine kinase MB isoforms and creatine kinase MB mass in early diagnosis of myocardial infarction in patients with acute chest pain.

    abstract:OBJECTIVES:Measurements of myoglobin and creatine kinase (CK)-MB isoforms have been suggested to be sensitive tests for the early diagnosis of myocardial infarction (MI). We have investigated the utility of myoglobin, creatine kinase (CK)-MB isoforms and creatine kinase MB mass (CK-MBm) in early diagnosis of MI using c...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(02)00385-5

    authors: Penttilä K,Koukkunen H,Halinen M,Rantanen T,Pyörälä K,Punnonen K,Penttilä I

    更新日期:2002-11-01 00:00:00

  • Apolipoprotein E polymorphism in the Tunisian population: frequency and effect on lipid parameters.

    abstract:OBJECTIVES:We determined the frequencies of apolipoprotein E (apo E) gene alleles and examined the association between apo E polymorphism and lipid parameters in a sample of the Tunisian population. DESIGN AND METHODS:Apo E polymorphism was investigated using PCR, and plasma lipid parameters were measured in 122 men a...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2006.04.018

    authors: Jemaa R,Elasmi M,Naouali C,Feki M,Kallel A,Souissi M,Sanhaji H,Hadj Taïeb S,Souheil O,Kaabachi N

    更新日期:2006-08-01 00:00:00

  • Random uncertainty of photometric determination of hemolysis index on the Abbott Architect c16000 platform.

    abstract:BACKGROUND:Automatic photometric determination of the hemolysis index (HI) on serum and plasma samples is central to detect potential interferences of in vitro hemolysis on laboratory tests. When HI is above an established cut-off for interference, results may suffer from a significant bias and undermine clinical relia...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2018.01.009

    authors: Aloisio E,Carnevale A,Pasqualetti S,Birindelli S,Dolci A,Panteghini M

    更新日期:2018-07-01 00:00:00

  • Cortisol and cortisone analysis in serum and plasma by atmospheric pressure photoionization tandem mass spectrometry.

    abstract:OBJECTIVES:Cortisol metabolism is controlled by 11beta-hydroxysteroid dehydrogenase (11beta-HSD) isoenzymes, which interconvert cortisol and cortisone. Accurate measurement of the cortisol and cortisone concentrations and their ratio provide useful information about 11beta-HSD activity. METHODS:Cortisol and cortisone ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2004.01.005

    authors: Kushnir MM,Neilson R,Roberts WL,Rockwood AL

    更新日期:2004-05-01 00:00:00

  • Association of parental hyperhomocysteinemia and C677T Methylene tetrahydrofolate reductase (MTHFR) polymorphism with recurrent pregnancy loss.

    abstract:OBJECTIVES:To investigate the association of parental hyperhomocysteinemia, C677T Methylene tetrahydrofolate reductase (MTHFR) polymorphism and DNA damage with recurrent pregnancy loss (RPL). DESIGN AND METHODS:A case-control study. Reverse phase HPLC, PCR-RFLP and Cytokinesis blocked micronuclei assay were used to as...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2008.12.003

    authors: Govindaiah V,Naushad SM,Prabhakara K,Krishna PC,Radha Rama Devi A

    更新日期:2009-03-01 00:00:00

  • Purification and characterization of immunosuppressive (IS) substance obtained from ascitic fluids of patients with gastrointestinal cancer.

    abstract::An immunosuppressive substance was isolated from ascitic fluids of patients with advanced colon cancer by means of ammonium sulphate precipitation and preparative isoelectric focusing. This was a glycoprotein with an isoelectric point of pH 2.7-3.3, a molecular weight of about 52,000, and a sedimentation coefficient o...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(87)80118-2

    authors: Fujii M,Takahashi N,Hayashi H,Matsunaga K,Yoshikumi C

    更新日期:1987-06-01 00:00:00

  • Single-strand conformational polymorphisms (SSCP): studies of the genetic polymorphisms of exon 4 of apolipoprotein C III.

    abstract:OBJECTIVE:We used single-strand conformational polymorphism (SSCP). To screen for mutations/polymorphisms in exon 4 of the apolipoprotein C III in 45 patients with hypertriglyceridemia and 46 control individuals, single-strand conformational polymorphism was investigated using restriction endonuclease and amplification...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/0009-9120(94)00083-8

    authors: Beheshti I,Hanson NQ,Copeland KR,Garg U,Tsai MY

    更新日期:1995-06-01 00:00:00

  • Predictors of inexplicable coronary artery spasm during coronary angiography in patients with stable angina--the role of intravascular oxidative stress.

    abstract:OBJECTIVE:This study was conducted to investigate the potential role of intravascular oxidative stress in inexplicable episodes of coronary artery spasm (CAS) during coronary angiography (CAG). DESIGN AND METHODS:Serial patients with stable angina scheduled for routine CAG were prospectively evaluated. Patients with c...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2009.01.021

    authors: Leu HB,Lin WT,Lin CP,Wu TC,Lin SJ,Chen JW

    更新日期:2009-05-01 00:00:00

  • The clinical relevance of imatinib plasma trough concentrations in chronic myeloid leukemia. A Belgian study.

    abstract::This retrospective multicenter study in patients with chronic myeloid leukemia in chronic phase was undertaken to confirm the clinical relevance of imatinib plasma concentrations monitoring in daily practice. Forty-one patients, with 47 imatinib plasma measurements, were analyzed during treatment with imatinib given a...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,多中心研究

    doi:10.1016/j.clinbiochem.2016.12.006

    authors: Van Obbergh F,Knoops L,Devos T,Beguin Y,Graux C,Benghiat F,Kargar-Samani K,Bauwens D,Efira A,Dubois C,Springael C,Montfort L,Connerotte T,Capron A,Delannoy A,Wallemacq P

    更新日期:2017-05-01 00:00:00

  • An overview of the kallikrein gene families in humans and other species: emerging candidate tumour markers.

    abstract::Kallikreins are serine proteases with diverse physiologic functions. They are represented by multigene families in many animal species, especially in rat and mouse. Recently, the human kallikrein gene family has been fully characterized and includes 15 members, tandemly localized on chromosome 19q13.4. A new definitio...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/s0009-9120(03)00055-9

    authors: Yousef GM,Diamandis EP

    更新日期:2003-09-01 00:00:00

  • Plasma and peripheral leukocyte beta-N-acetylhexosaminidase isoenzymes and disease activity in rheumatoid arthritis.

    abstract:OBJECTIVES:Recently it has been suggested that serum beta-N-acetylhexosaminidase (Hex) could be a joint destruction marker in rheumatoid arthritis (RA) patients. However, a large amount of serum Hex activity has its source from platelets, and the blood platelet-count is often increased in RA, which may have masked the ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(02)00339-9

    authors: Casal JA,Mera A,Pérez LF,Tutor JC

    更新日期:2002-09-01 00:00:00

  • Detection of circulating tumor cells in peripheral blood from patients with gastric cancer using piRNAs as markers.

    abstract:OBJECTIVE:To investigate the feasibility of detecting circulating cancer cells in peripheral blood from gastric cancer patients using Piwi-interacting RNAs (piRNAs) as markers. DESIGN AND METHODS:Real-time quantitative reverse transcription-polymerase chain reaction was used to analyze piR-651 and piR-823 levels in th...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2011.06.004

    authors: Cui L,Lou Y,Zhang X,Zhou H,Deng H,Song H,Yu X,Xiao B,Wang W,Guo J

    更新日期:2011-09-01 00:00:00