Apolipoprotein E polymorphism in the Tunisian population: frequency and effect on lipid parameters.


OBJECTIVES:We determined the frequencies of apolipoprotein E (apo E) gene alleles and examined the association between apo E polymorphism and lipid parameters in a sample of the Tunisian population. DESIGN AND METHODS:Apo E polymorphism was investigated using PCR, and plasma lipid parameters were measured in 122 men and 111 women aged 35 to 87 years. RESULTS:The allele frequencies were epsilon2: 7.3%, epsilon3: 84.6%, and epsilon4: 8.1%. Apo E polymorphism was associated with significant differences (P<0.001) in total cholesterol, apo B and LDL cholesterol in both men and women. epsilon2 carriers had the lowest mean total cholesterol, apo B and LDL-C concentrations, and subjects with the epsilon4 allele had the highest levels. Triglycerides levels increased with the epsilon4 allele, but this did not reach statistical significance. These results remained unchanged after adjustment for age, body mass index, sex, hypertension, diabetes and smoking. However, in obese subjects (BMI>30 kg/m2), TG concentrations were significantly lower in individuals homozygous for the epsilon3 allele compared to those with the alleles epsilon2 or epsilon4. CONCLUSION:In this sample of the Tunisian population, the distribution of apo E gene alleles is similar to that observed in Southern European populations with low prevalence of the epsilon4 allele. Variations in the apo E gene play a role in determining plasma lipid levels. These data also suggest that effects of apo E alleles on lipids levels are partly dependent on environmental variables such as BMI. These findings highlight the importance of the gene/environment interaction on the deleterious effect of obesity on cardiovascular risk factors.


Clin Biochem


Clinical biochemistry


Jemaa R,Elasmi M,Naouali C,Feki M,Kallel A,Souissi M,Sanhaji H,Hadj Taïeb S,Souheil O,Kaabachi N




Has Abstract


2006-08-01 00:00:00














  • Head-to-head comparison of an automated immunometric and an automated HPLC method for the quantification of HbA1c.

    abstract:OBJECTIVES:To compare a fully automated immunoassay and a fully automated HPLC method for the quantification of HbA1c. DESIGN AND METHODS:For an inter-method comparison, 200 consecutive clinical samples were analyzed. Imprecision was studied with pooled whole blood. RESULTS:Very close agreement of results (identical ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Vogeser M,Parhofer KG

    更新日期:2008-11-01 00:00:00

  • Is there an analytical or diagnostic advantage from including trisialo transferrin into the fraction of carbohydrate-deficient transferrin? Lessons from a comparison of two commercial turbidimetric immunoassays with the carbohydrate-deficient transferrin

    abstract:OBJECTIVES:Carbohydrate-deficient transferrin CDT has originally been defined as the sum of isotransferrins exhibiting isoelectric point values > or = 5.7 asialo, monosialo, and disialo transferrin but may also include at least in part trisialo transferrin when measured by modern commercial immunoassays. To examine the...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Lipkowski M,Dibbelt L,Seyfarth M

    更新日期:2000-11-01 00:00:00

  • Comparison of cytokeratin 20 RNA and angiogenin in voided urine samples as diagnostic tools for bladder carcinoma.

    abstract:BACKGROUND:We evaluated the diagnostic efficacy of urinary angiogenin (ANG) and cytokeratin 20 (CK-20) mRNA in comparison with voided urine cytology in the detection of bladder cancer patients. OBJECTIVES AND METHODS:A total of 97 Egyptian patients provided a single voided urine sample for ANG, CK-20 and cytology befo...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Eissa S,Kenawy G,Swellam M,El-Fadle AA,Abd El-Aal AA,El-Ahmady O

    更新日期:2004-09-01 00:00:00

  • Ornithine methyl ester. An unusual metabolite encountered in the urine of patients with a urea cycle disorder characterized by hyperammonemia, hyperornithinemia and homocitrullinuria.

    abstract::In the urine of six subjects with a urea cycle disorder characterized by hyperammonemia, hyperornithinemia and homocitrullinuria, an unusual ninhydrin-reaction compound was encountered. This unknown on hydrolysis yielded ornithine as the only amino acid and, on dansylation studied, yielded didansyl ornithine. The meta...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Gordon BA,Gatfield PD,Taller E

    更新日期:1977-04-01 00:00:00

  • Low normal thyroid function as a determinant of increased large very low density lipoprotein particles.

    abstract:OBJECTIVES:Low-normal thyroid function may relate to increases in plasma cholesterol and triglycerides, but effects on lipoprotein subfractions are largely unknown. Associations of alterations in lipoprotein metabolism and functionality with low-normal thyroid function could be more pronounced in Type 2 diabetes mellit...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: van Tienhoven-Wind L,Dullaart RP

    更新日期:2015-05-01 00:00:00

  • Relationship between measured average glucose by continuous glucose monitor and HbA1c measured by three different routine laboratory methods.

    abstract:OBJECTIVES:The relationship between glycated hemoglobin A1c (HbA1c) and average glucose has been described by the empirically derived estimated average glucose (eAG) equation in the A1c-Derived Average Glucose (ADAG) study, with extensive calibration efforts in four secondary reference HbA1c methods. It is not known if...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Loh TP,Sethi SK,Wong MS,Tai ES,Kao SL

    更新日期:2015-05-01 00:00:00

  • Comparison of new solid-phase extraction methods for chromatographic identification of drugs in clinical toxicological analysis.

    abstract:OBJECTIVES:Newly presented solid-phase extraction methods (Solid Phase Disc Extraction, SPEC-Plus, Multi-Modal, and Solid Phase Micro Extraction, SPME have been checked with respect to their applicability to clinical toxicological analysis. In comparison with conventional liquid/liquid-extraction and a common mixed-pha...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Degel F

    更新日期:1996-12-01 00:00:00

  • Markedly increased serum aspartate aminotransferase activity in congestive heart failure.

    abstract::Congestive heart failure usually features a mild to moderate increase in aspartate aminotransferase (AST) activity. A marked increase in AST may occur rarely and is usually preceded by prolonged shock or hypotensive episodes and is accompanied by hyperbilirubinemia. I report two patients with congestive heart failure ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Nanji AA

    更新日期:1983-04-01 00:00:00

  • Comparative assessment of matrix metalloproteinase (MMP)-2 and MMP-9, and their inhibitors, tissue inhibitors of metalloproteinase (TIMP)-1 and TIMP-2 in preeclampsia and gestational hypertension.

    abstract:OBJECTIVES:To compare the circulating levels of matrix metalloproteinase (MMP)-2, MMP-9, tissue inhibitors of metalloproteinase (TIMP)-1, TIMP-2, and the MMP-9/TIMP-1 and MMP-2/TIMP-2 ratios in preeclampsia and gestational hypertension with those found in normotensive pregnancies. DESIGN AND METHODS:We studied 83 preg...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Palei AC,Sandrim VC,Cavalli RC,Tanus-Santos JE

    更新日期:2008-07-01 00:00:00

  • Tentative identification of hemoglobin variants in the Bio-Rad VARIANT II Hb A1C method.

    abstract:OBJECTIVE:To evaluate if peak characteristics, the retention time, and percentage of total hemoglobin of an unknown peak on the Bio-Rad VARIANT II Hb A1c method may be used to establish a tentative identification of hemoglobin variants. METHOD:The peak characteristics, retention time, and percentage of total hemoglobi...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Higgins TN,Ridley B

    更新日期:2005-03-01 00:00:00

  • In vitro and in vivo cell-capture strategies using cardiac stent technology - A review.

    abstract::Stenosis is a symptom of coronary artery disease (CAD), and is caused by narrowing of arteries in the heart. Over the last several decades, medical implants such as cardiac stents have been developed to counter stenosis. Upon implantation of a stent to open up a restricted artery, narrowing of the artery can reoccur (...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审


    authors: Ravindranath RR,Romaschin A,Thompson M

    更新日期:2016-01-01 00:00:00

  • Biobanking 3.0: evidence based and customer focused biobanking.

    abstract::Biobanking is a new and very dynamic field. To achieve long term financial sustainability of biobank infrastructures we propose that a new focus is needed on activities, products and services provided by the biobank that relate to the external stakeholder: biobanking 3.0. Earlier stages of biobanking are biobanking 1....

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Simeon-Dubach D,Watson P

    更新日期:2014-03-01 00:00:00

  • Establishing reference intervals for hCG in postmenopausal women.

    abstract:BACKGROUND:Plasma concentrations of human chorionic gonadotropin (hCG) have been shown to increase with age due to pituitary secretion. We previously recommended that an hCG cutoff of 14.0IU/L be used for women ≥55years of age. However, it remains unknown whether concentrations >14.0IU/L can be expected in women with a...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Patel KK,Qavi AJ,Hock KG,Gronowski AM

    更新日期:2017-03-01 00:00:00

  • Serum HER-2/neu in the management of breast cancer patients.

    abstract::The clinical role of HER-2/neu, a 185 kD epithelial transmembranous protein, has evolved after the approval of the anti-HER-2/neu targeted monoclonal antibody trastuzumab (Herceptin) for the therapy of metastatic breast cancer. The extracellular domain of HER-2/neu undergoes proteolytic cleavage from the full-length p...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审


    authors: Lüftner D,Lüke C,Possinger K

    更新日期:2003-06-01 00:00:00

  • Recent advances in head and neck squamous cell carcinoma--a review.

    abstract::The current review presents the results of the most recent studies performed on different aspects of human head and neck squamous cell carcinoma, including radiosensitivity induction, efficiency improvement of monoclonal antibodies using low-intensity ultrasound, chemical compounds such as toll-like receptor (TLC) ago...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审


    authors: Safdari Y,Khalili M,Farajnia S,Asgharzadeh M,Yazdani Y,Sadeghi M

    更新日期:2014-09-01 00:00:00

  • Therapy in adenosine deaminase and purine nucleoside phosphorylase deficient patients.

    abstract::The discovery of the causal association of adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP) deficiency with some forms of primary immunodeficiency disease had led to new approaches to therapy, such as enzyme replacement. In ADA deficiency, bone marrow transplantation remains the primary method of ch...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审


    authors: Zegers BJ,Stoop JW

    更新日期:1983-02-01 00:00:00

  • Plasma and urine betaine and dimethylglycine variation in healthy young male subjects.

    abstract:OBJECTIVES:We aimed to compare the individuality (within subject consistency) of plasma and urine betaine and N,N-dimethylglycine. DESIGN AND METHODS:In two separate groups of 8 males (ages 19 to 40), plasma (10) and urine (6) samples were collected either over a single day or over an 8 week period. The individuality ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Lever M,Atkinson W,Slow S,Chambers ST,George PM

    更新日期:2009-05-01 00:00:00

  • Genotyping of the G1138A mutation of the FGFR3 gene in patients with achondroplasia using high-resolution melting analysis.

    abstract:OBJECTIVES:The fibroblast growth factor receptor 3 gene (FGFR3) plays a critical role in cartilage growth-plate differentiation and bony development. It has been shown that 97% of patients with achondroplasia have a G to A transition mutation at position 1138 (c.1138 G>A) of codon 380 of the FGFR3 gene. DESIGN AND MET...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Hung CC,Lee CN,Chang CH,Jong YJ,Chen CP,Hsieh WS,Su YN,Lin WL

    更新日期:2008-02-01 00:00:00

  • Inappropriate repeat testing of complete blood count (CBC) and electrolyte panels in inpatients from Alberta, Canada.

    abstract:INTRODUCTION:The avoidance of repeat chemistry testing such as Complete Blood Count (CBC) and Electrolyte Panel (EP) on clinically stable patients was identified as important utilization goals by Choosing Wisely Canada. The purpose of this study was to assess the volume of overutilization of CBC and EP in an inpatient ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Kandalam V,Lau CK,Guo M,Ma I,Naugler C

    更新日期:2020-03-01 00:00:00

  • Lateral flow assay for simultaneous detection of cellular- and humoral immune responses.

    abstract:OBJECTIVE:The development of a cytokine detection assay suitable for detection of multiple biomarkers for improved diagnosis of mycobacterial diseases. DESIGN AND METHODS:A lateral flow (LF) assay to detect IL-10 was developed utilizing the up-converting phosphor (UCP) reporter-technology. The assay was evaluated usin...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Corstjens PL,de Dood CJ,van der Ploeg-van Schip JJ,Wiesmeijer KC,Riuttamäki T,van Meijgaarden KE,Spencer JS,Tanke HJ,Ottenhoff TH,Geluk A

    更新日期:2011-10-01 00:00:00

  • Enhanced reduction in oxidative stress and altered glutathione and thioredoxin system response to unsaturated fatty acid load in familial hypercholesterolemia.

    abstract:OBJECTIVES:Familial hypercholesterolemia (FH) is characterized by increased oxidative stress (OS) levels. In the postprandial state, lipids and lipoproteins modulate OS status through their impact on pro-oxidant and antioxidant mechanisms. The objective of this study was to evaluate in patients with FH the response to ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Cortes R,Martinez-Hervas S,Ivorra C,De Marco G,Gonzalez-Albert V,Rojo-Martínez G,Saez G,Carmena R,Ascaso JF,Real JT,Chaves FJ

    更新日期:2014-12-01 00:00:00

  • Monoclonal antibody technology for cyclosporine monitoring.

    abstract::Monitoring blood levels of Cyclosporine (CsA) has been the basis for adjusting individual dosage regimens in the clinic. Radioimmunoassays using polyclonal antisera reacted with CsA and some CsA metabolites, leading to overestimation when compared with high-performance liquid chromatographic measurements of CsA. Monoc...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审


    authors: Quesniaux VF

    更新日期:1991-02-01 00:00:00

  • Evaluation and assessment of high sensitivity thyrotropin methods as an index for thyroid function.

    abstract::We report the evaluation of four new commercially available sensitive assay kits for determination of thyrotropin (TSH) and their clinical utility in normal subjects and patients with thyroidal and non-thyroidal illnesses. The sensitivity for the reliable detection of serum TSH by these methods ranged from 0.1-0.4 mU/...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Kalra J,Laxdal VA,Walker SD,Massey KL

    更新日期:1987-10-01 00:00:00

  • Effect of repeated freeze-thaw cycles on maternal serum biological markers for the detection of fetal trisomies.

    abstract:OBJECTIVES:We studied the stability of maternal blood markers for screening for Down syndrome (alpha-fetoprotein, unconjugated estriol, intact human chorionic gonadotropin (hCG) and free beta-human chorionic gonadotropin) upon repeated freeze-thaw cycles. DESIGN AND METHODS:Forty-three samples collected from second tr...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Massé J,Forest JC,Moutquin JM,Degrandpre P

    更新日期:1997-10-01 00:00:00

  • Water bath hyperthermia reduces stemness of colon cancer cells.

    abstract:OBJECTIVES:Researchers have proposed that cancer stem cells (CSCs) are the driving force of tumorigenesis, recurrence, and metastases, contributing to the failure of some cancer treatments. Water bath hyperthermia, a novel treatment for cancer, can be highly effective when combined with chemotherapy, radiotherapy, or i...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Gao F,Ye Y,Zhang Y,Yang J

    更新日期:2013-11-01 00:00:00

  • Vitamins D and A can be successfully measured by LC-MS/MS in cord blood diluted plasma.

    abstract:OBJECTIVES:In widely used protocols for the collection and isolation of cord blood mononuclear cells, investigators are left with substantial volumes of diluted plasma which could be used for other measurements. The aim of this study was to ascertain the validity of umbilical cord blood (UCB) diluted plasma samples for...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Albarhani AA,Collier F,Greaves RF,Ponsonby AL,Allen KJ,Vuillermin PJ,Roche P,Clarke MW,BIS Steering Committee.

    更新日期:2015-11-01 00:00:00

  • Analytical interference of 4-hydroxy-3-methoxymethamphetamine with the measurement of plasma free normetanephrine by ultra-high pressure liquid chromatography-tandem mass spectrometry.

    abstract:OBJECTIVES:The diagnosis of pheochromocytoma relies on the measurement of plasma free metanephrines assay whose reliability has been considerably improved by ultra-high pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Here we report an analytical interference occurring between 4-hydroxy-3-methoxym...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Dunand M,Donzelli M,Rickli A,Hysek CM,Liechti ME,Grouzmann E

    更新日期:2014-08-01 00:00:00

  • Occupational lead exposure: studies in two brothers showing differential susceptibility to lead.

    abstract::Unexpected differences in clinical and biochemical findings in two brothers occupationally exposed to the same source of lead for dissimilar lengths of time are presented. Only the brother with the shorter period of lead exposure was anemic and afflicted by nausea, vomiting, abdominal colic and arthralgia. His urinary...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Haust HL,Poon HC,Inwood M,O'Hea EK,Haines DS,Forret CJ,Keys DS

    更新日期:1985-04-01 00:00:00

  • CRISPR-based tools: Alternative methods for the diagnosis of COVID-19.

    abstract::The recently emerged severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) spread all over the world rapidly and caused a global pandemic. To prevent the virus from spreading to more individuals, it is of great importance to identify and isolate infected individuals through testing. Reverse transcription-quanti...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审


    authors: Palaz F,Kalkan AK,Tozluyurt A,Ozsoz M

    更新日期:2021-01-09 00:00:00

  • Measurement of oxidized lipoprotein (a) in patients with acute coronary syndromes and stable coronary artery disease by 2 ELISAs: using different capture antibody against oxidized lipoprotein (a) or oxidized LDL.

    abstract:OBJECTIVE:To evaluate clinical value of oxidized lipoprotein(a) [ox-Lp(a)] levels. DESIGN AND METHODS:Ox-Lp(a) were measured by 2 ELISAs using antibodies against ox-Lp(a) [ox-Lp(a)1] or oxidized low-density lipoprotein [ox-Lp(a)2], and studied in 161 acute coronary syndromes (ACS) patients, 114 stable coronary artery ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Wang JJ,Han AZ,Meng Y,Gong JB,Zhang CN,Li K,Liu YX

    更新日期:2010-04-01 00:00:00