Immunoassays with time-resolved fluorescence spectroscopy: principles and applications.

abstract：:1. A CK(BB) fraction was observed in sera of a patient with prostatic carcinoma. The fraction accounted for 59% of the total enzyme activity. A metastatic tumor of a prostatic carcinoma was observed to contain solely the CK(BB) isozyme. An embryonal testicular carcinoma was observed to have a higher percentage of the ...

journal_title：Clinical biochemistry

authors： Hoag G,Amies DR,Colquhoun BD

更新日期：1978-04-01 00:00:00

abstract：OBJECTIVES:Stable isotope dilution coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) is the sensitive method for screening for various inherited metabolic disorders using dried blood spots (DBSs). We present a method for LC-MS/MS determination of succinyladenosine (SAdo) and succinylaminoimidazole ...

journal_title：Clinical biochemistry

authors： Zikanova M,Krijt J,Skopova V,Krijt M,Baresova V,Kmoch S

更新日期：2015-01-01 00:00:00

abstract：:A method is described for the determination of low plasma levels of adenosine-5'-diphosphate (ADP) using a Dupont Biometer to measure luminescence produced by the luciferin-luciferase reaction. Endogenous ATP is removed by incubation with luciferase. The remaining ADP is then quantitated, following its conversion to A...

journal_title：Clinical biochemistry

authors： Jabs CM,Ferrell WJ,Robb HJ

更新日期：1978-10-01 00:00:00

abstract：OBJECTIVES:The ratio of glycated albumin to HbA1c (GA/HbA1c ratio) is a known indicator that reflects fluctuations in plasma glucose. In this study, the association of the GA/HbA1c ratio to diabetic nephropathy and diabetic retinopathy in patients with type 2 diabetes was investigated. DESIGN AND METHODS:Among patient...

journal_title：Clinical biochemistry

authors： Umayahara Y,Fujita Y,Watanabe H,Kasai N,Fujiki N,Hatazaki M,Koga M

更新日期：2017-04-01 00:00:00

abstract：OBJECTIVES:Oxidative stress has a clear pro tumoral effect in myeloproliferative neoplasms (MPDs). In this study, we analyzed oxidative stress in patients with essential thrombocythemia (ET) and polycythemia vera (PV). Design and methods We analyzed serum levels of advanced oxidation protein products (AOPPs) degradatio...

journal_title：Clinical biochemistry

authors： Musolino C,Allegra A,Saija A,Alonci A,Russo S,Spatari G,Penna G,Gerace D,Cristani M,David A,Saitta S,Gangemi S

更新日期：2012-11-01 00:00:00

abstract：OBJECTIVES:Drug screening in neonates is traditionally performed using meconium, but cord tissue has been proposed as an alternative specimen. This study compares the detection of drugs in a large number of paired meconium and umbilical cord tissue samples from subjects at risk of in utero drug exposure. DESIGN AND ME...

journal_title：Clinical biochemistry

authors： Colby JM

更新日期：2017-09-01 00:00:00

abstract：:An immunosuppressive substance was isolated from ascitic fluids of patients with advanced colon cancer by means of ammonium sulphate precipitation and preparative isoelectric focusing. This was a glycoprotein with an isoelectric point of pH 2.7-3.3, a molecular weight of about 52,000, and a sedimentation coefficient o...

journal_title：Clinical biochemistry

authors： Fujii M,Takahashi N,Hayashi H,Matsunaga K,Yoshikumi C

更新日期：1987-06-01 00:00:00

abstract：OBJECTIVES:The newly developed glomerular filtration rate (GFR)-estimating equations developed by the CKD-EPI Collaboration and Feng et al. (2013) that are based on standardized serum cystatin C (ScysC), combined/not combined with serum creatinine (Scr), require further validation in China. We compared the performance ...

journal_title：Clinical biochemistry

authors： Guo X,Qin Y,Zheng K,Gong M,Wu J,Shou W,Cheng X,Xia L,Xu E,Li X,Qiu L

更新日期：2014-09-01 00:00:00

abstract：:Enzymic fluorimetric methods are described for the determination of primary bile acids and of chenodeoxycholic acid (CDC) and cholic acid (C) in serum. Bile acids are extracted from 0.3 mL of serum in a simple 5-min step with use of Sep-Pak C cartridges. Total primary bile acids are measured by an equilibrium techniqu...

journal_title：Clinical biochemistry

authors： Papanastasiou-Diamandi A,Diamandis EP,Soldin SJ

更新日期：1984-08-01 00:00:00

abstract：:This review focusses on research performed by the author and coworkers. The absorption, turnover and excretion of cobalamin and the pathogenesis of cobalamin deficiency states are described and the laboratory tests used to diagnose these states are discussed. Topics dealt with in detail include: overall turnover, dail...

journal_title：Clinical biochemistry

authors： Gräsbeck R

更新日期：1984-04-01 00:00:00

abstract：OBJECTIVES:In joint degenerative diseases, the collagens are degraded by matrix metalloproteinases and protein fragments are released to serum as potential biomarkers. METHODS:A collagen type II specific neoepitope, CIIM, was identified (…RDGAAG(1053)) by mass spectrometry. Two ELISAs against the neoepitope were devel...

journal_title：Clinical biochemistry

authors： Bay-Jensen AC,Liu Q,Byrjalsen I,Li Y,Wang J,Pedersen C,Leeming DJ,Dam EB,Zheng Q,Qvist P,Karsdal MA

更新日期：2011-04-01 00:00:00

abstract：:A spectrophotometric study was carried out for three proteins assays when modification of their serum procedures using bromcresol green, bromcresol purple and biuret reagents were applied to the determinations of total proteins and albumin in cerebrospinal fluids. A novel concentration device wherein the sample itself...

journal_title：Clinical biochemistry

authors： Artiss JD,Thibert RJ,Zak B

更新日期：1981-02-01 00:00:00

abstract：OBJECTIVES:Biallelic mutations in the ARG1 gene result in an uncommon autosomal recessive inborn defect of the urea cycle known as hyperargininemia (OMIM #207800). ARG1 splicing mutations are not reported often, and they are probably related to a more severe phenotype than missense mutations. In this article, we descri...

journal_title：Clinical biochemistry

authors： Villegas-Ruiz V,Campos-Garcia FJ,Contreras-Capetillo S,Moreno-Graciano CM,Maldonado-Solis FA,Maldonado-Solis MA,Zenteno JC

更新日期：2015-12-01 00:00:00

abstract：:The serum argino-succinate lyase (ASAL) activity was measured in preoperative and post-operative serum samples, from approximately 500 patients submitted to elective surgery. The results were compared with the determinations of 6 other enzyme activities on the same serum specimens. Serum ASAL elevations correlated hig...

journal_title：Clinical biochemistry

authors： Sims FH,Rautanen P

更新日期：1975-06-01 00:00:00

abstract：OBJECTIVE:The purpose of this study was to develop an improved method of direct DNA sequencing, which makes it possible to identify heterozygous mutations of the lipoprotein lipase (LPL) gene in order to understand the underlying genetic disorder of type IV hyperlipoproteinemia. METHODS AND RESULTS:The direct sequenci...

journal_title：Clinical biochemistry

authors： Mori A,Takagi A,Ikeda Y,Yamamoto A

更新日期：1997-06-01 00:00:00

abstract：BACKGROUND:L-Dopa decarboxylase (DDC) is a pyridoxal 5'-phosphate-dependent enzyme that was found to be involved in many malignancies. The aim of this study was to investigate the mRNA expression levels of DDC in prostate tissues and to evaluate its clinical utility in prostate cancer (CaP). METHODS:Total RNA was isol...

journal_title：Clinical biochemistry

authors： Avgeris M,Koutalellis G,Fragoulis EG,Scorilas A

更新日期：2008-10-01 00:00:00

abstract：:We describe a simple and reliable radioimmunoassay (RIA) method for the measurement of human placental lactogen (hPL) in undiluted serum. Standard curves, in serum, cover the range of 0.015 to 1 mlU/L for early pregnancy monitoring (7 to 15 weeks, referred to as Procedure I), and 0.531 to 17 mIU/L for mid and late pre...

journal_title：Clinical biochemistry

authors： Englebienne P,Doyen G,Dervaux R,Billemont JP

更新日期：1982-04-01 00:00:00

abstract：OBJECTIVE:Determine the relation of xanthine oxidase (XO) activity and the outcome of septic patients and its relation to oxidative damage and clinical parameters of sepsis severity. DESIGN AND METHODS:Patients admitted over a 6-month period were enrolled. Patients were assigned to groups according to the diagnosis of...

journal_title：Clinical biochemistry

authors： Luchtemberg MN,Petronilho F,Constantino L,Gelain DP,Andrades M,Ritter C,Moreira JC,Streck EL,Dal-Pizzol F

更新日期：2008-10-01 00:00:00

abstract：OBJECTIVE:To confirm that the naproxen metabolite O-desmethylnaproxen interferes with the Jendrassik and Grof method for total bilirubin and was a possible cause of spurious hyperbilirubinemia in a case of naproxen overdose. DESIGN AND METHODS:Plasma was spiked with naproxen or O-desmethylnaproxen before total and dir...

journal_title：Clinical biochemistry

authors： Al Riyami N,Zimmerman AC,Rosenberg FM,Holmes DT

更新日期：2009-01-01 00:00:00

abstract：:The initial step in the action of thyrotropin (TSH) is its binding to the TSH receptor. TSH receptor antibodies are detected in up to 90% of patients with Graves' disease. Serial measurements of TSH receptor antibodies in patients with Graves' hyperthyroidism are helpful in predicting relapse. The TSH receptor was pur...

journal_title：Clinical biochemistry

authors： Harrison LC,Leedman PJ

更新日期：1990-02-01 00:00:00

abstract：OBJECTIVES:High density lipoprotein (HDL)-associated paraoxonase-1 (PON-1) exerts anti-oxidative properties, whereas phospholipid transfer protein (PLTP) is able to convert mature HDL into larger and smaller HDL particles. Here we tested associations of PON-1 with PLTP in type 2 diabetes mellitus (T2DM), a condition ch...

journal_title：Clinical biochemistry

authors： Dullaart RPF,Gruppen EG,Dallinga-Thie GM

更新日期：2016-04-01 00:00:00

abstract：BACKGROUND:Cardiac troponins (cTn) are essential in the diagnostic assessment of non-ST-segment-elevation acute coronary syndrome (NSTE-ACS). Elevated concentrations of cTnT and cTnI predict cardiovascular events in non-acute settings, but the individual troponin isotype association with long-term mortality in patients...

journal_title：Clinical biochemistry

authors： Tveit SH,Myhre PL,Hoff NJS,Le TM,Seljeflot I,Røysland R,Høiseth AD,Røsjø H,Omland T

更新日期：2020-04-01 00:00:00

abstract：INTRODUCTION:Barricor™ Vacutainers® are a novel non-gel separator blood collection tube. These tubes enable faster pre-analytical processing which could reduce turnaround time and be beneficial in an acute care setting. We sought to evaluate the bias, stability, and integrity of plasma generated from these tubes compar...

journal_title：Clinical biochemistry

authors： Fournier JE,Northrup V,Clark C,Fraser J,Howlett M,Atkinson P,Shea JL

更新日期：2018-08-01 00:00:00

abstract：:We describe a simple direct extraction method for the gas-liquid chromatography determination of serum valproic acid. The working range for the assay is 2-180 mg/L and our within-run precision was 5.8 and 4.3% at the 40 and 90 mg/L concentrations respectively. Hemolyzed and lipemic sera as well as samples from patient...

journal_title：Clinical biochemistry

authors： Leroux M,Budnik D,Hall K,Irvine-Meek J,Otten N,Seshia S

更新日期：1981-04-01 00:00:00

abstract：OBJECTIVE:To determine Retinol Binding Protein 4 (RBP4) levels in patients with Familial Partial Lipodystrophy (FPLD). METHODS:Ten patients with FPLD and a control group (9 patients) were selected to participate in the study. RESULTS:RBP4-log levels were lower in patients with FPLD in comparison to control group (1.5...

journal_title：Clinical biochemistry

authors： Godoy-Matos AF,Moreira RO,MacDowell R,Bendet I,Mory PB,Moises RS

更新日期：2009-07-01 00:00:00

abstract：:We assessed the diagnostic value of four commercially available methods for determining pancreatic lipase (LPS) in serum (the turbidimetric procedure from Boehringer, two enzymatic approaches from Kodak and Poli, and an immunochemical assay) in a population of 46 hospitalized patients with acute abdominal pain. In 31 ...

journal_title：Clinical biochemistry

authors： Panteghini M,Pagani F,Bonora R,Alebardi O,Ceriotti F

更新日期：1991-12-01 00:00:00

abstract：BACKGROUND:In standard pre-analytical conditions, an isolation step is required for circulating tumor DNA (ctDNA) analysis. The need for this step remains unclear with the development of ultrasensitive detection technologies such as digital PCR (dPCR). The aim of our study was to evaluate the ctDNA detection by dPCR pl...

journal_title：Clinical biochemistry

authors： Sefrioui D,Beaussire L,Perdrix A,Clatot F,Michel P,Frebourg T,Di Fiore F,Sarafan-Vasseur N

更新日期：2017-11-01 00:00:00

abstract：OBJECTIVES:Hepcidin is the main regulator of systemic iron homeostasis and its expression is modulated by iron status, hypoxia, erythroid factors and inflammation. The aim of our study was to examine a relationship between level of hepcidin and iron status, erythropoietic activity, hypoxia and inflammation in exacerbat...

journal_title：Clinical biochemistry

authors： Tandara L,Grubisic TZ,Ivan G,Jurisic Z,Tandara M,Gugo K,Mladinov S,Salamunic I

更新日期：2015-12-01 00:00:00

abstract：OBJECTIVE:Elevated concentrations of hCG beta core fragment (hCGβcf) are known to cause false-negative results in qualitative urine pregnancy test devices, but the pattern of urinary hCGβcf during normal pregnancy has not been well characterized. Here, we evaluate the relationship between urine hCG, hCGβcf, and hCG fre...

journal_title：Clinical biochemistry

authors： Nerenz RD,Yarbrough ML,Stenman UH,Gronowski AM

更新日期：2016-07-01 00:00:00

abstract：OBJECTIVES:To test for specific mutations in the alanine:glyoxylate aminotransferase (AGT) gene, in order to diagnose primary hyperoxaluria type 1 (PH1). DESIGN AND METHODS:Samples of liver and/or DNA from 81 patients were submitted to our laboratory for diagnostic testing for PH1. Using a panel of selected mutations,...

journal_title：Clinical biochemistry

authors： Coulter-Mackie MB,Lian Q,Applegarth DA,Toone J,Waters PJ,Vallance H

更新日期：2008-05-01 00:00:00