当前位置： SCI文献检索 > CLINICAL BIOCHEMISTRY期刊下所有文献 > Enzyme-linked immunosorbent assay (ELISAs) for metalloproteinase derived type II collagen neoepitope, CIIM--increased serum CIIM in subjects with severe radiographic osteoarthritis.

Enzyme-linked immunosorbent assay (ELISAs) for metalloproteinase derived type II collagen neoepitope, CIIM--increased serum CIIM in subjects with severe radiographic osteoarthritis.

Abstract：

OBJECTIVES:In joint degenerative diseases, the collagens are degraded by matrix metalloproteinases and protein fragments are released to serum as potential biomarkers. METHODS:A collagen type II specific neoepitope, CIIM, was identified (…RDGAAG(1053)) by mass spectrometry. Two ELISAs against the neoepitope were developed. CIIM was measured in cartilage explants in the presence or absence of protease inhibitors. CIIM was measured in OA synovial fluid (n=51) and serum (n=156). Knee OA was graded by standard Kellgren-Lawrence (KL) score. RESULTS:The ELISAs showed good technical performance; CV%, <13%. CIIM release from cartilage explants was blocked by the MMP inhibitor. CIIM was detected in synovial fluid. Furthermore, serum CIIM levels were significantly higher (P<0.05) in those individuals with mild or severe OA than in those with no OA. CONCLUSION:We developed a new biomarker for joint degenerative diseases, which we demonstrated was derived from MMP-degraded type II collagen.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Bay-Jensen AC,Liu Q,Byrjalsen I,Li Y,Wang J,Pedersen C,Leeming DJ,Dam EB,Zheng Q,Qvist P,Karsdal MA

doi

10.1016/j.clinbiochem.2011.01.001

subject

Has Abstract

pub_date

2011-04-01 00:00:00

pages

423-9

issue

5-6

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(11)00019-1

journal_volume

44

pub_type

杂志文章

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