Abstract：
INTRODUCTION:Vitamin B12 deficiency is mostly caused by insufficient gastro-intestinal absorption and in rare conditions by Transcobalamin (TC) deficiency. Unsaturated Transcobalamin (apoTC) can be measured by a binding assay using radiolabeled cobalamin. The Active B12 test analyzes saturated Transcobalamin (holoTC) and we hypothesize that this test can be used to measure total TC by additional in vitro saturation with cobalamin. METHODS:Serum was saturated in vitro (16 times dilution) with a cyanocobalamin solution and total TC was selectively measured with the Abbott Active B12 test. ApoTC was calculated by subtracting endogenous holoTC from total TC after correction for dilution. Linearity was determined with a pool serum dilution series. Precision was investigated according to the CLSI EP15 protocol. Method comparison was performed against a binding assay using radiolabeled cobalamin. Reference values were determined in 100 healthy controls. RESULTS:The method was linear in the range of 240 to 1933pmol/L (R2=0.997, lack of fit F=1.61). Precision of low- and high-pool total TC in serum were; 5.2% and 4.3% respectively. Method comparison against a radiolabeled cobalamin binding assay showed a proportional bias of 30% (y=0.70x+126). Total TC reference values were determined at 500-1276pmol/L. CONCLUSION:We describe a rapid method to quantify total TC, which can be implemented on routine platforms using commercial Active B12 tests. In addition, apoTC can be assessed by subtracting endogenous holoTC concentration which can be measured in the same run, securing the same calibration level for all three parameters (holoTC, apoTC and total TC). This method is applicable in clinical diagnostics and in larger epidemiological studies.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Griffioen PH,van Dam-Nolen DHK,Lindemans J,Heil SGdoi
10.1016/j.clinbiochem.2017.08.009subject
Has Abstractpub_date
2017-12-01 00:00:00pages
1030-1033issue
18eissn
0009-9120issn
1873-2933pii
S0009-9120(17)30528-3journal_volume
50pub_type
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journal_title：Clinical biochemistry
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更新日期：2014-04-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2004-12-01 00:00:00
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更新日期：2014-10-01 00:00:00
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