Abstract:
OBJECTIVE:Protein glutathionylation is considered an important post-translational modification in the pathogenesis of complex diseases. The aim of this study was to examine whether hemoglobin (Hb) is modified by reduced glutathione (GSH) via oxidation of the thiol groups present in diabetes and its associated microangiopathy and to determine whether oxidative imbalance has any correlation with glutathionylated Hb (HbSSG) levels. METHODS:The study group consisted of a total of 130 subjects which included non-diabetic healthy control subjects (n = 30) and type 2 diabetic patients with (n = 53) and without (n = 47) microangiopathy. All subjects were assessed for glycemic and lipidemic status, while diabetic subjects were also assessed for the diagnosis of retinopathy and nephropathy. RBC lysates from all the subjects were analyzed by liquid chromatography/electrospray ionization-mass spectrometry (LC/ESI-MS) for HbSSG beta-globin chains. Levels of GSH and thiobarbituric acid substances (TBARS) levels were measured by spectrophotometric and fluorimetric methods, respectively. RESULTS:The positivity for HbSSG in diabetic subjects with microangiopathy was significantly higher (69%) compared to diabetics without microangiopathy (22%) and control subjects (14%). In univariate regression analysis, HbSSG levels were significantly associated with the duration of diabetes, HbA1c, and TBARS levels. GSH levels were negatively correlated (r = -0.57, P < 0.001) with HbSSG in diabetic subjects. A significant inverse correlation (r = -0.42, P < 0.001) between the GSH levels and HbA1c levels was also seen in diabetic subjects. CONCLUSIONS:This is perhaps the largest LC-MS-based study to demonstrate that HbSSG levels are markedly increased in diabetic subjects with microangiopathy. Since diabetic subjects also exhibited increased lipid peroxidation and decreased GSH levels, it appears that enhanced oxidative stress may account for the increased HbSSG concentrations and altered reduction-oxidation (redox) signaling.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Sampathkumar R,Balasubramanyam M,Sudarslal S,Rema M,Mohan V,Balaram Pdoi
10.1016/j.clinbiochem.2005.06.009subject
Has Abstractpub_date
2005-10-01 00:00:00pages
892-9issue
10eissn
0009-9120issn
1873-2933pii
S0009-9120(05)00168-2journal_volume
38pub_type
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journal_title:Clinical biochemistry
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(78)90641-0
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abstract::Inherited and acquired changes in pre-mRNA splicing have been documented to play a significant role in human disease development and many cancer-associated genes are regulated by alternative splicing. Loss of fidelity, variation of the splicing process, even controlled switching to specific splicing alternatives may o...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
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更新日期:2004-07-01 00:00:00
abstract:BACKGROUND:Cystinosis is a rare autosomal recessive disorder characterized by an accumulation of intralysosomal cystine due to a defect in cystine transport across the lysosomal membrane. This disorder can be treated specifically using high doses of cysteamine. Accurate measurement of intracellular cystine content is n...
journal_title:Clinical biochemistry
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更新日期:2002-02-01 00:00:00
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pub_type: 杂志文章,meta分析
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更新日期:1995-06-01 00:00:00
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doi:10.1016/j.clinbiochem.2008.03.017
更新日期:2008-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0009-9120(78)90070-x
更新日期:1978-06-01 00:00:00
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doi:10.1016/s0009-9120(79)80139-3
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pub_type: 杂志文章
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更新日期:1985-02-01 00:00:00
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更新日期:2007-01-01 00:00:00
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更新日期:2011-01-01 00:00:00
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更新日期:2010-07-01 00:00:00
abstract:OBJECTIVE:The development of a cytokine detection assay suitable for detection of multiple biomarkers for improved diagnosis of mycobacterial diseases. DESIGN AND METHODS:A lateral flow (LF) assay to detect IL-10 was developed utilizing the up-converting phosphor (UCP) reporter-technology. The assay was evaluated usin...
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更新日期:2011-10-01 00:00:00
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更新日期:2020-12-01 00:00:00
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更新日期:2009-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2007.07.014
更新日期:2007-11-01 00:00:00
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更新日期:2008-12-01 00:00:00
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更新日期:2011-04-01 00:00:00
abstract:BACKGROUND:Vitamin D (25-OHD) physiological functions have been expanded beyond traditional bone health, increasing the importance of its estimation in Laboratory Medicine, which renders validation of available methods mandatory. AIMS AND METHODS:We evaluated some preanalytical and analytical aspects of 25-OHD determi...
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更新日期:2012-12-01 00:00:00
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更新日期:2017-01-01 00:00:00