Abstract:
BACKGROUND:The aldosterone/renin ratio (ARR) is recommended to screen for primary aldosteronism (PA) in hypertension. We estimated fully automated chemiluminescence immunoassays (CLIA) for plasma aldosterone concentrations (PAC) and plasma direct renin concentrations (PRC) and investigated their reference intervals in Chinese Han population. METHODS:PAC and PRC were measured on a fully automated analyzer (LIAISON XL, DiaSorin, Italy). Performance characteristics were estimated according to CLSI approved guidelines. 328 healthy individuals were selected for reference intervals investigation. Results simultaneously tested by CLIA and radioimmunoassays were reviewed from 123 patients with hypertension and/or adrenal space-occupying lesion. PAC/PRC ratio (ARRprc) was compared to PAC/plasma renin activity (PRA) ratio (ARRpra). RESULTS:Within-laboratory imprecision was 5.6%-6.7% for PAC and 3.0%-3.3% for PRC. The LoQ was 72.2 pmol/L for PAC and 1.27 mIU/L for PRC. Linearity was excellent in the range of concentrations between 94 and 2708 pmol/L for PAC and 1.3-461.8 mIU/L for PRC. Interferences of hemoglobin, unconjugated bilirubin and lipaemia could be acceptable, but not of conjugated-bilirubin when renin and aldosterone at low concentrations. The central 95% reference intervals for males: PAC: 76-722 pmol/L, PRC: 3.3-92.7 mIU/L, ARR: 2.2-46.0 pmol/mIU; for females: PAC: 85-1010 pmol/L, PRC: 3.7-99.8 mIU/L, ARR: 3.6-68.4 pmol/mIU. Upper reference limits for ARR of younger and older men were lower than women. ARRprc and ARRpra showed almost perfect agreement (kappa = 0.815) for screening PA. CONCLUSION:The DiaSorin tests are valuable analytical options for PAC and PRC measurements. We recommend sex-specific and age-specific reference intervals of these items should be estimated.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Deng L,Xiong Z,Li H,Lei X,Cheng Ldoi
10.1016/j.clinbiochem.2018.04.016subject
Has Abstractpub_date
2018-06-01 00:00:00pages
89-94eissn
0009-9120issn
1873-2933pii
S0009-9120(18)30113-9journal_volume
56pub_type
杂志文章abstract::An affinity chromatographic method (AC) was compared with ion exchange chromatography (IEC) for determination of glycated hemoglobin (gHb). In vitro, increasing glucose concentration produced no change on AC-determined gHb. gHb measurement by AC was highly correlated with mean blood glucose (r = 0.96). Mean values of ...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(86)80010-8
更新日期:1986-12-01 00:00:00
abstract:OBJECTIVES:High-resolution melting analysis has been validated for screening several genes of clinical significance. We evaluated whether it would be useful for detecting mutations in the LDL receptor gene (LDLR). DESIGN AND METHODS:We analysed 60 samples heterozygous for different LDLR sequence variants, and compared...
journal_title:Clinical biochemistry
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doi:10.1016/j.clinbiochem.2008.11.015
更新日期:2009-04-01 00:00:00
abstract::The effect of altered thyroid function on serum fructosamine concentrations was investigated in 31 untreated hyperthyroid patients, 18 short-term hypothyroid patients (i.e., 20 days after withdrawal of thyroid hormone suppressive therapy for thyroid cancer), 7 untreated long-term hypothyroid patients, and 25 age-match...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/0009-9120(88)90007-0
更新日期:1988-06-01 00:00:00
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journal_title:Clinical biochemistry
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doi:10.1016/j.clinbiochem.2013.03.005
更新日期:2013-06-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(99)00003-x
更新日期:1999-04-01 00:00:00
abstract::The use of alcohol and tobacco is prevalent among pregnant women despite the well-known adverse effects of these substances imposed on the developing fetus and immense public health education efforts. Confirmation of gestational exposures to these compounds have relied mostly on maternal self-reporting, which is often...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2004.01.010
更新日期:2004-06-01 00:00:00
abstract::This study was performed to investigate the antioxidant mechanisms of polymorphonuclear leucocytes (PMN) in active stage of Behçet's Disease. PMN activities of myeloperoxidase (p < 0.02), superoxide dismutase (p < 0.001), catalase (p < 0.005), and glutathione peroxidase (p < 0.005) were significantly lower in the pati...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/0009-9120(94)90046-9
更新日期:1994-10-01 00:00:00
abstract:OBJECTIVES:Elevated advanced glycation endproducts (AGEs) are implicated in diabetic complications. Methylglyoxal-derived hydroimidazolone (MG-H) is one of the most abundant AGEs in vivo. Our objective was to develop a time-saving, specific method to measure free MG-H in plasma and determine its levels in complication-...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2008.12.016
更新日期:2009-05-01 00:00:00
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journal_title:Clinical biochemistry
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更新日期:1997-12-01 00:00:00
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journal_title:Clinical biochemistry
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更新日期:2009-09-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2007.12.013
更新日期:2008-04-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章,多中心研究
doi:10.1016/0009-9120(93)90017-z
更新日期:1993-02-01 00:00:00
abstract::The biosynthesis of prostate specific antigen (PSA) was studied in human epidermoid carcinoma, (KB) cells and in normal human embryonic lung (WI-38)cells. The prostate carcinoma cell line, PC-35F12 was used as a control. PSA specific antibodies were used to precipitate the immunologically reactive peptides from cell e...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(01)00280-6
更新日期:2001-11-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2011.05.029
更新日期:2011-08-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2008.02.017
更新日期:2008-06-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2004.11.004
更新日期:2005-03-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2017.02.018
更新日期:2017-08-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2014.05.064
更新日期:2014-09-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2012.09.001
更新日期:2012-12-01 00:00:00
abstract::During sequential physiologic maturation of an individual follicle, the number of granulosa cells increases in excess of 1000-fold, while intra-ovarian concentrations of sex steroids escalate by 100-fold. The recent development of several in vitro ovarian systems has permitted a more extensive and direct assessment of...
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pub_type: 杂志文章,评审
doi:10.1016/s0009-9120(81)90976-0
更新日期:1981-10-01 00:00:00
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pub_type: 杂志文章,多中心研究
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更新日期:2018-08-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2006.04.018
更新日期:2006-08-01 00:00:00
abstract::Bone metabolism is assessed using biochemical bone turnover markers (BTM). BTM reflect the metabolic effect of drugs on bone turnover, help to establish the lowest dose inducing the largest change in the BTM, predict treatment-related reduction in fracture risk, and are helpful in bridging studies. Changes in BTM duri...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2012.01.022
更新日期:2012-08-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2012.10.020
更新日期:2013-01-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2017.10.002
更新日期:2018-02-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 临床试验,杂志文章
doi:10.1016/s0009-9120(05)80009-8
更新日期:1991-12-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(79)80125-3
更新日期:1979-12-01 00:00:00
abstract::Osteopontin (OPN) is a highly phosphorylated glycophosphoprotein having acidic characteristics and rich in aspartic acid. OPN, a multifunctional protein, has important functions on cardiovascular diseases, cancer, diabetes and kidney stone diseases and in the process of inflammation, biomineralization, cell viability ...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2018.07.003
更新日期:2018-09-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2016.11.005
更新日期:2017-03-01 00:00:00
abstract:OBJECTIVES:Regulatory T cells (Tregs) which may indicate operational tolerance provide a promising biomarker for individualization of immunosuppression. Naturally thymus-derived Tregs (nTregs) represent the major suppressive phenotype and can be identified by their demethylation status in the Tregs Specific Demethylate...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2016.05.019
更新日期:2016-10-01 00:00:00