The measurement of serum cholinesterase activities by an integration strategy with expanded linear ranges and negligible substrate-activation.

abstract：BACKGROUND:The availability of reference intervals is essential for physicians to interpret laboratory results. Most of our laboratory reference intervals are derived from data on the foreign population. We have studied the reference intervals of 24 common laboratory biochemical tests in an apparently healthy adult Han...

journal_title：Clinical biochemistry

authors： Yang S,Qiao R,Li Z,Wu Y,Yao B,Wang H,Cui L,Yang Y,Zhang J

更新日期：2012-10-01 00:00:00

abstract：:An ion-exchange chromatographic procedure is described which facilitates the determination of beta-chain aminoterminal modified glycated hemoglobin. The procedure includes an erythrocyte lysis reagent which eliminates the labile aldimine component (pre-A1c) and a two-stage elution step which separates HbA, 1a + b from...

journal_title：Clinical biochemistry

authors： Jaynes PK,Willis MC,Chou PP

更新日期：1985-02-01 00:00:00

abstract：BACKGROUND:Measurement of 25(OH)D has evolved rapidly, with an increased number of high-throughput automated immunoassays becoming available in recent years. The aim of this study was to fully evaluate six commercially available automated 25(OH)D immunoassays, including 2 newly available (Beckman Coulter) and one recal...

journal_title：Clinical biochemistry

authors： Wyness SP,Straseski JA

更新日期：2015-11-01 00:00:00

abstract：BACKGROUND:The aldosterone/renin ratio (ARR) is recommended to screen for primary aldosteronism (PA) in hypertension. We estimated fully automated chemiluminescence immunoassays (CLIA) for plasma aldosterone concentrations (PAC) and plasma direct renin concentrations (PRC) and investigated their reference intervals in ...

journal_title：Clinical biochemistry

authors： Deng L,Xiong Z,Li H,Lei X,Cheng L

更新日期：2018-06-01 00:00:00

abstract：OBJECTIVES:Pseudoxanthoma elasticum (PXE) is a hereditary disorder predominantly affecting the skin, retina and vascular system. The aim of this study was to measure cell adhesion molecules in PXE patients. DESIGN AND METHODS:Soluble P-, E- and L-selectins were measured in 61 non-consanguineous PXE patients. The distr...

journal_title：Clinical biochemistry

authors： Götting C,Adam A,Szliska C,Kleesiek K

更新日期：2008-04-01 00:00:00

abstract：OBJECTIVE:Direct measurement of chemical constituents in complex biologic matrices without the use of analyte specific reagents could be a step forward toward the simplification of clinical biochemistry. Problems related to reagents such as production errors, improper handling, and lot-to-lot variations would be elimin...

journal_title：Clinical biochemistry

authors： Jessen TE,Höskuldsson AT,Bjerrum PJ,Verder H,Sørensen L,Bratholm PS,Christensen B,Jensen LS,Jensen MA

更新日期：2014-09-01 00:00:00

abstract：:A commercially available assay (Sigma) for urinary oxalates based on the oxalate oxidase/peroxidase catalyzed reaction produced, in our hands, recoveries of added oxalate that varied from patient to patient. A modification of the assay is reported that involves the chromatographic separation of interfering substances,...

journal_title：Clinical biochemistry

authors： Mazzuchin A,Michelutti L,Falter H

更新日期：1990-04-01 00:00:00

abstract：OBJECTIVES:Oxidative stress has a clear pro tumoral effect in myeloproliferative neoplasms (MPDs). In this study, we analyzed oxidative stress in patients with essential thrombocythemia (ET) and polycythemia vera (PV). Design and methods We analyzed serum levels of advanced oxidation protein products (AOPPs) degradatio...

journal_title：Clinical biochemistry

authors： Musolino C,Allegra A,Saija A,Alonci A,Russo S,Spatari G,Penna G,Gerace D,Cristani M,David A,Saitta S,Gangemi S

更新日期：2012-11-01 00:00:00

abstract：OBJECTIVES:To develop a tandem mass spectrometry (MS/MS) method for plasma free and total carnitine, incorporating acid hydrolysis for total carnitine measurement, for improved accuracy and sample throughput. DESIGN AND METHODS:Limit of detection (LOD), limit of quantitation (LOQ), linearity, accuracy, precision coeff...

journal_title：Clinical biochemistry

authors： Johnson DW

更新日期：2010-11-01 00:00:00

abstract：OBJECTIVES:CA 15-3 and CEA are considered useful tumor markers in monitoring breast cancer patients. This study was undertaken to specifically evaluate the transient elevations in these markers that are observed during systemic treatment for metastatic disease. This phenomenon has been termed "spiking." DESIGN AND MAT...

journal_title：Clinical biochemistry

authors： Yasasever V,Dinçer M,Camlica H,Karaloğlu D,Dalay N

更新日期：1997-02-01 00:00:00

abstract：:The synthesis and characterisation of a new colorimetric reagent for iron, 3-(2-pyridyl)-5,6-bis(2-furyl)-1,2,4-triazine are described. Peak assignments and chemical shifts of the high resolution 13C nmr spectrum are given. The reagent forms a tris iron(II) complex with epsilon max of 32000 Lcm-1 mol-1 at 577 nm. The ...

journal_title：Clinical biochemistry

authors： Smith FE,Herbert J,Gaudin J,Hennessy DJ,Reid GR

更新日期：1984-10-01 00:00:00

abstract：OBJECTIVES:We have previously identified three minor immunophilins of molecular weights 37 kDa, 14 kDa, and 5-8 kDa capable of binding tacrolimus and sirolimus. DESIGN AND METHODS:When tested against pure preparations of five sirolimus metabolites, the 14 kDa protein had almost no cross-reactivity, the 37 kDa protein ...

journal_title：Clinical biochemistry

authors： Davis DL,Murthy JN,Gallant-Haidner H,Yatscoff RW,Soldin SJ

更新日期：2000-02-01 00:00:00

abstract：:Our studies lead us to suggest that in order for an organic chemical to cause porphyrin accumulation in chick embryo hepatocyte culture, it must be: 1. lipophilic; 2. resistant to biotransformation by phase I or phase II reactions to compounds which are devoid of biological activity; and 3. capable of interacting with...

journal_title：Clinical biochemistry

authors： Marks GS,McCluskey SA,Mackie JE,Riddick DS,James CA

更新日期：1989-06-01 00:00:00

abstract：:We investigated the effects of medium supplementation with increasing amounts of different fatty acids on HepG2 cell cholesterol biosynthesis and esterification. Up to 200 microM 16:0 led to an increase in cholesterol secretion/synthesis of 60%/40%; 18:1, 18:2, or 18:3 decreased secretion/synthesis by 35%/25%, 65%/65%...

journal_title：Clinical biochemistry

authors： Schäfer HF,Kattermann R

更新日期：1992-10-01 00:00:00

abstract：:1. Six subjects were investigated during the luteal phase of the menstrual cycle to determine whether the ovarian steroids, estrogen and progesterone were released in an episodic manner. 2. Analysis of plasma obtained every 20 min. during a normal working day provided no evidence for pulsatile release of either steroi...

journal_title：Clinical biochemistry

authors： Younglai EV,Smith SL,Cleghorn JM,Streiner DL

更新日期：1975-08-01 00:00:00

abstract：OBJECTIVES:Development of an ELISA for human insulin that utilizes monoclonal antibodies (mAbs) produced in serum-free medium. DESIGN AND METHODS:Insulin mAbs were produced in vitro by hybridomas in serum-free medium. A two-step ELISA was developed to replace bovine insulin (standard) and bovine serum albumin (assay b...

journal_title：Clinical biochemistry

authors： Even MS,Sandusky CB,Barnard ND,Mistry J,Sinha MK

更新日期：2007-01-01 00:00:00

abstract：OBJECTIVE:Heat-mediated ELISA (HELISA) technique has been reported to shorten ELISA timings and has major clinical implications in diagnostic field [Bora U, Kannan K, Nahar P. Heat mediated enzyme linked immunosorbent assay. J Immunol Methods 2004; 293: 43-50]. Objective of this study is to find out whether anti-MBL au...

journal_title：Clinical biochemistry

authors： Sharma P,Gupta B,Farhat Basir S,Rani Das H,Nahar P

更新日期：2008-01-01 00:00:00

abstract：:To elucidate the diagnostic relevance of biliary conjugated bilirubin, biliary bilirubin from normal volunteers (NV), patients with Gilbert's syndrome (GS) and Crigler-Najjar syndrome type II (C-N II), and from various rat strains was fractionated. Biliary bilirubin diglucuronide (BDG) was present at lower levels, and...

journal_title：Clinical biochemistry

authors： Adachi Y,Yamashita M,Nanno T,Yamamoto T

更新日期：1990-04-01 00:00:00

abstract：:Enzymic fluorimetric methods are described for the determination of primary bile acids and of chenodeoxycholic acid (CDC) and cholic acid (C) in serum. Bile acids are extracted from 0.3 mL of serum in a simple 5-min step with use of Sep-Pak C cartridges. Total primary bile acids are measured by an equilibrium techniqu...

journal_title：Clinical biochemistry

authors： Papanastasiou-Diamandi A,Diamandis EP,Soldin SJ

更新日期：1984-08-01 00:00:00

abstract：OBJECTIVES:In this study we aimed to assess the changes in pro-oxidant-antioxidant balance (PAB) after the placement of either a drug-eluting-stent (DES) or bare-metal-stent (BMS) in patients with stable coronary artery disease. DESIGN AND METHODS:Percutaneous coronary interventions (PCI) with either BMS or DES were u...

journal_title：Clinical biochemistry

authors： Falsoleiman H,Dehghani M,Moohebati M,Fazlinezhad A,Daloee MH,Alamdari DH,Esmaily HO,Parizadeh SM,Tavallaie S,Rahsepar AA,Paydar R,Kazemi-Bajestani SM,Pourghadam-Yari H,Parizad PA,Ghayour-Mobarhan M,Ferns GA

更新日期：2011-02-01 00:00:00

abstract：:Drug therapy may produce toxicity. Patient individuality or drug interactions account for many cases of poisoning, but other factors such as genetic predisposition, drug contamination or human error are also known causes. Examples of various types of drug poisoning are given, illustrating the role of the clinical chem...

journal_title：Clinical biochemistry

authors： Stewart MJ

更新日期：1990-02-01 00:00:00

abstract：:We have evaluated the precision of the Ektachem 400 thin film enzymic creatinine method and compared it to that obtained for an AutoAnalyzer II (AAII) picric acid procedure. The results of the study show that the Ektachem method yields imprecise creatinine values over the low concentration range (40-240 mumol/L). Run-...

journal_title：Clinical biochemistry

authors： Jacklyn CL,Matte SJ,Ryder DI,Cousins CL,Moss MA

更新日期：1985-12-01 00:00:00

abstract：INTRODUCTION:The avoidance of repeat chemistry testing such as Complete Blood Count (CBC) and Electrolyte Panel (EP) on clinically stable patients was identified as important utilization goals by Choosing Wisely Canada. The purpose of this study was to assess the volume of overutilization of CBC and EP in an inpatient ...

journal_title：Clinical biochemistry

authors： Kandalam V,Lau CK,Guo M,Ma I,Naugler C

更新日期：2020-03-01 00:00:00

abstract：:Human liver arginase has many biological effects on lymphocytes, macrophages, liver cells, and tumor cells, in addition to its major role in the liver urea cycle. We have developed a sandwich enzyme-linked immunosorbent assay (ELISA) method to quantitate arginase concentrations in plasma that can be applied to various...

journal_title：Clinical biochemistry

authors： Wang SR,Chen ML,Huang MH,Lin HY,Tsai JJ,Kuo BI

更新日期：1993-12-01 00:00:00

abstract：OBJECTIVE:To confirm that the naproxen metabolite O-desmethylnaproxen interferes with the Jendrassik and Grof method for total bilirubin and was a possible cause of spurious hyperbilirubinemia in a case of naproxen overdose. DESIGN AND METHODS:Plasma was spiked with naproxen or O-desmethylnaproxen before total and dir...

journal_title：Clinical biochemistry

authors： Al Riyami N,Zimmerman AC,Rosenberg FM,Holmes DT

更新日期：2009-01-01 00:00:00

abstract：:This review discusses the question: which steps are essential or need to be optimized so that maternal plasma RNA sequencing for pre-eclampsia and related syndromes will become as specific and sensitive as maternal plasma DNA sequencing for trisomy? ...

journal_title：Clinical biochemistry

authors： Oudejans CB

更新日期：2015-10-01 00:00:00

abstract：:To design and deliver high quality, safe and effective products, manufacturers of in vitro diagnostic (IVD) products follow a structured, traceable process for controlling the uncertainty of results reported from their measurement systems. This process and its results however, are not often shared in detail with those...

journal_title：Clinical biochemistry

authors： Orzechowski A,Petrides V,Scopp R

更新日期：2018-07-01 00:00:00

abstract：OBJECTIVES:Although numerous recent studies have focused on analyses of microRNA expression profiles in cancer cells, the expression patterns of the enzymes responsible for the generation of miRNAs remains largely unexplored. The purpose of this study was to investigate whether Dicer mRNA expression is altered during p...

journal_title：Clinical biochemistry

authors： Pampalakis G,Diamandis EP,Katsaros D,Sotiropoulou G

更新日期：2010-02-01 00:00:00

abstract：BACKGROUND:Researchers are debating if fecal calprotectin results are useful for infant patients, especially in screening for necrotizing enterocolitis (NEC). Currently, none of the FDA-approved calprotectin assays provide a cut-off for infant patients. We retrospectively analyzed data from a reference laboratory and u...

journal_title：Clinical biochemistry

authors： Goold E,Pearson L,Johnson LM

更新日期：2020-10-01 00:00:00

abstract：OBJECTIVES:We determined the relationship of smoking status on APOC3 -482C>T polymorphism and apolipoprotein C-III (apoC-III) concentrations and the latter two parameters' influence on risk of diabetes and coronary heart disease (CHD). DESIGN AND METHODS:Prediction of incident cases was assessed at 5.5years' follow-up...

journal_title：Clinical biochemistry

authors： Onat A,Erginel-Unaltuna N,Coban N,Ciçek G,Yüksel H

更新日期：2011-04-01 00:00:00