Serum retinol binding protein 4 in patients with familial partial lipodystrophy.

abstract：:We report a sensitive and specific method for the determination of AY-25,712 concentrations in rat, dog and human serum by liquid chromatography with UV detection. The detection limit is 25 ng/mL, based on 2 mL of serum or plasma. The method has been validated in human volunteers receiving single oral doses of AY-25,7...

journal_title：Clinical biochemistry

authors： Gonzalez R,Thibeault D

更新日期：1983-08-01 00:00:00

abstract：:Three commercially available assays (an enzyme-linked immunosorbent assay ELISA, an immunoradiometric assay, IRMA, and a nephelometric assay) for the determination of lipoprotein(a) [Lp(a)] were compared with respect to the dependency of these assays on the various apolipoprotein(a) [apo(a)] isoforms. Although there w...

journal_title：Clinical biochemistry

authors： Leus FR,Leerink CB,Prins J,van Rijn HJ

更新日期：1994-12-01 00:00:00

abstract：:Rheumatoid factors (RFs) have been studied for over 50 years and are probably the most written about of any antibody. Nevertheless, the etiology of these RFs and the precise role they play in the pathogenesis of rheumatoid arthritis (RA) and juvenile rheumatoid arthritis (JRA) remain a major interest. When RFs partici...

journal_title：Clinical biochemistry

authors： Moore TL,Dorner RW

更新日期：1993-04-01 00:00:00

abstract：OBJECTIVES:Sphingosine 1-phosphate (S1P) is carried in plasma by the HDL particles and albumin. It mediates several protective functions of HDL. Because of its barrier-enhancing effect, it has attracted attention in diseases associated with endothelial dysfunction. We examined the impact of circulating levels of S1P in...

journal_title：Clinical biochemistry

authors： Bekpinar S,Yenidunya G,Gurdol F,Unlucerci Y,Aycan-Ustyol E,Dinccag N

更新日期：2015-12-01 00:00:00

abstract：OBJECTIVE:MicroRNA-331 (miR-331) has shown regulatory activity against several genes whose expression has been claimed to be deregulated in breast tumors, including that of epidermal growth factor receptor 2 (HER2). Herein, the clinical value of miR-331 expression was investigated by analyzing its levels in breast beni...

journal_title：Clinical biochemistry

authors： Papadopoulos EI,Papachristopoulou G,Ardavanis A,Scorilas A

更新日期：2018-09-01 00:00:00

abstract：OBJECTIVES:The objectives of this study were to compare the concentrations, size profiles and major tissue contributors of cell-free DNA (cfDNA) in plasma and in serum. DESIGN AND METHODS:Thirteen pregnant women in the third trimester were recruited for this study. We collected EDTA-plasma and serum samples using vari...

journal_title：Clinical biochemistry

authors： Wong FC,Sun K,Jiang P,Cheng YK,Chan KC,Leung TY,Chiu RW,Lo YM

更新日期：2016-12-01 00:00:00

abstract：OBJECTIVES:We have previously identified three minor immunophilins of molecular weights 37 kDa, 14 kDa, and 5-8 kDa capable of binding tacrolimus and sirolimus. DESIGN AND METHODS:When tested against pure preparations of five sirolimus metabolites, the 14 kDa protein had almost no cross-reactivity, the 37 kDa protein ...

journal_title：Clinical biochemistry

authors： Davis DL,Murthy JN,Gallant-Haidner H,Yatscoff RW,Soldin SJ

更新日期：2000-02-01 00:00:00

abstract：:High-sensitivity cardiac troponin (hs-cTn) assays have been used clinically by thousands of physicians in many countries throughout the world since their clinical introduction seven years ago. In the early diagnosis of myocardial infarction (MI), beyond doubt the most important indication of hs-cTn assays, these simpl...

journal_title：Clinical biochemistry

authors： Twerenbold R,Boeddinghaus J,Nestelberger T,Wildi K,Rubini Gimenez M,Badertscher P,Mueller C

更新日期：2018-03-01 00:00:00

abstract：OBJECTIVES:To compare the circulating levels of cathepsin D in preeclamptic and normotensive pregnancies. DESIGN AND METHODS:Fifty pregnant and 20 healthy non-pregnant patients were enrolled in this study. Of the 50 pregnant patients, 15 were preeclamptic and 35 patients were normotensive. Serum levels of soluble cath...

journal_title：Clinical biochemistry

authors： Kim HY,Lee M,Kang HW,Moon C

更新日期：2013-12-01 00:00:00

abstract：:Altered urinary excretion of glycosaminoglycans (GAG) has been reported in patients with nephrolithiasis, with chronic glomerulonephritis, and incipient diabetic nephropathy, but evaluation of urinary GAG has not been reported in infections and proliferating diseases of the urinary tract. Urinary excretion of GAG was ...

journal_title：Clinical biochemistry

authors： Baggio B,Gambaro G,Cicerello E,Mastrosimone S,Marzaro G,Borsatti A,Pagano F

更新日期：1987-12-01 00:00:00

abstract：BACKGROUND:There is little information on temperature and period of freezing for generating hemolysate and blood cell lysate. MATERIALS AND METHODS:Primary tubes containing whole blood were frozen at -20°C and -80°C and then serially thawed to assess cell lysis and clinical chemistry tests in centrifuged plasma. RESU...

journal_title：Clinical biochemistry

authors： Lippi G,Musa R,Aloe R,Mercadanti M,Pipitone S

更新日期：2011-10-01 00:00:00

abstract：BACKGROUND:While investigating estrogen response element (ERE) binding properties of human estrogen receptor-α (hERα) in breast cancer cytosols, other ERE-binding proteins (ERE-BP) were observed. DESIGN AND METHODS:Recognition properties of ERE-BP were evaluated by electrophoretic mobility shift assays (EMSA) with ERE...

journal_title：Clinical biochemistry

authors： Kruer TL,Cummins TD,Powell DW,Wittliff JL

更新日期：2013-11-01 00:00:00

abstract：BACKGROUND:With the advent of the new high-sensitivity troponin assays, it is becoming critical to measure troponin accurately to low concentrations. To ensure assay performance is acceptable, appropriate QC must be run. METHODS:In addition to the routine use of commercial QC material, we prepared pools of human QC ma...

journal_title：Clinical biochemistry

authors： Hickman PE,Koerbin G,Badrick T,Oakman C,Potter JM

更新日期：2018-08-01 00:00:00

abstract：:We have evaluated the precision of the Ektachem 400 thin film enzymic creatinine method and compared it to that obtained for an AutoAnalyzer II (AAII) picric acid procedure. The results of the study show that the Ektachem method yields imprecise creatinine values over the low concentration range (40-240 mumol/L). Run-...

journal_title：Clinical biochemistry

authors： Jacklyn CL,Matte SJ,Ryder DI,Cousins CL,Moss MA

更新日期：1985-12-01 00:00:00

abstract：OBJECTIVES:Recurrent miscarriage (RM) is a challenging medical problem because of its unknown pathogenesis and etiology in most of the cases. Recent studies suggest the role of persistent environmental pollutants such as organochlorine pesticides (OCPs) in the etiology of RM. The present study was conducted to investig...

journal_title：Clinical biochemistry

authors： Pathak R,Mustafa M,Ahmed RS,Tripathi AK,Guleria K,Banerjee BD

更新日期：2010-01-01 00:00:00

abstract：OBJECTIVES:To determine the relationship between BiliChek TcB (Respironics, Marietta GA) and Doumas reference serum or plasma total bilirubin (TSB). DESIGN AND METHODS:Pooled samples with values assigned by the Doumas reference method were used to establish the relationship between a local laboratory and reference Dou...

journal_title：Clinical biochemistry

authors： Karon BS,Wickremasinghe AC,Lo SF,Saenger AK,Cook WJ

更新日期：2010-08-01 00:00:00

abstract：OBJECTIVES:Patients with Ehlers-Danlos syndrome were described to contain reduced activities of beta4-galactosyltransferase-7 (beta4Gal-T7). Therefore, measurement of beta4Gal-T7 activity can help to characterize defects in proteoglycan biosynthesis in patients with connective tissue diseases. DESIGN AND METHODS:We de...

journal_title：Clinical biochemistry

authors： Kuhn J,Kleesiek K,Götting C

更新日期：2009-04-01 00:00:00

abstract：BACKGROUND:Plasma concentrations of human chorionic gonadotropin (hCG) have been shown to increase with age due to pituitary secretion. We previously recommended that an hCG cutoff of 14.0IU/L be used for women ≥55years of age. However, it remains unknown whether concentrations >14.0IU/L can be expected in women with a...

journal_title：Clinical biochemistry

authors： Patel KK,Qavi AJ,Hock KG,Gronowski AM

更新日期：2017-03-01 00:00:00

abstract：:Monitoring blood levels of Cyclosporine (CsA) has been the basis for adjusting individual dosage regimens in the clinic. Radioimmunoassays using polyclonal antisera reacted with CsA and some CsA metabolites, leading to overestimation when compared with high-performance liquid chromatographic measurements of CsA. Monoc...

journal_title：Clinical biochemistry

authors： Quesniaux VF

更新日期：1991-02-01 00:00:00

abstract：OBJECTIVES:Classical galactosemia (McKusick 230400) is an autosomal recessive disorder caused by mutations in the galactose-1-phosphate uridyl transferase (GALT;EC 2.7.7.10) gene. DESIGN AND METHODS:In the present study, we report molecular analysis of 14 unrelated Iranian galactosemia children with reduced or without...

journal_title：Clinical biochemistry

authors： Mirzajani F,Mirfakhraie R,Nabati F,Tabatabaei NN,Talachian E,Houshmand M

更新日期：2006-07-01 00:00:00

abstract：OBJECTIVES:Angiotensin converting enzyme (ACE), G-Protein couple receptor (G-Prot), endothelial nitric oxide synthase (ecNOS), Leptin -2548G/A and uncoupling protein (UCP2) are potent regulators of intra renal hemodynamics and may be the causative factors contributing to the deterioration of renal functions. In recent ...

journal_title：Clinical biochemistry

authors： Tripathi G,Sharma RK,Baburaj VP,Sankhwar SN,Jafar T,Agrawal S

更新日期：2008-05-01 00:00:00

abstract：AIMS:There is increasing interest in using fructosamine measurements in screening for or managing diabetes, yet uncertainty remains as to whether these measurements should be corrected for variation in serum protein concentrations. METHODS:We considered all sets of simultaneous measurements of fructosamine, albumin, t...

journal_title：Clinical biochemistry

authors： Rodríguez-Segade S,Rodríguez J,Camiña F

更新日期：2017-02-01 00:00:00

abstract：OBJECTIVE:We examined the association of plasma apolipoprotein (apoB) B-48, remnant-like particle (RLP)-cholesterol and non-HDL cholesterol concentrations with apoB-100 kinetics in overweight-obese men. METHODS AND RESULTS:Very-low density lipoprotein (VLDL), intermediate-density lipoprotein (IDL) and low-density lipo...

journal_title：Clinical biochemistry

authors： Chan DC,Watts GF,Ng TW,Uchida Y,Sakai N,Yamashita S,Barrett PH

更新日期：2005-09-01 00:00:00

abstract：OBJECTIVES:A conformation sensitive gel electrophoresis (CSGE) method was modified for detection of LDL receptor gene mutations. DESIGN AND METHODS:Usage of a temperature gradient against running time was tested with 33 identified mutations in the LDL receptor gene. Most of the mutations were missense. RESULTS:32 mut...

journal_title：Clinical biochemistry

authors： Fard-Esfahani P,Khatami S,Zeinali C,Taghikhani M,Allahyari M

更新日期：2005-06-01 00:00:00

abstract：OBJECTIVES:To evaluate platelet activating factor (PAF) levels, its metabolic enzymes activity and its associations with other inflammatory markers in heart failure (HF) patients. DESIGN AND METHODS:PAF, and two of its key biosynthetic enzymes [lyso-PAF acetyltransferase (lyso-PAF-AT) and DTT-insensitive CDP-choline:1...

journal_title：Clinical biochemistry

authors： Detopoulou P,Nomikos T,Fragopoulou E,Antonopoulou S,Kotroyiannis I,Vassiliadou C,Panagiotakos DB,Chrysohoou C,Pitsavos C,Stefanadis C

更新日期：2009-01-01 00:00:00

abstract：OBJECTIVES:Matrix-assisted laser desorption time-of flight ionization (MALDI)-imaging MS (IMS) with MSMS analysis using on-tissue tryptic digests is a powerful tool for identification of disease-related proteins in formalin-fixed paraffin-embedded (FFPE) tissue sections. We applied this novel IMS technique, not only to...

journal_title：Clinical biochemistry

authors： Nakanishi T,Ito M,Nirasawa T,Tsuji M,Takubo T

更新日期：2013-10-01 00:00:00

abstract：OBJECTIVES:The relative proportion in percentage of the isoenzyme A of beta-hexosaminidase (Hex) is the single discriminatory function most frequently used for the biochemical screening of heterozygote Tay-Sachs disease carriers. It has been suggested that the assay of the Hex isoenzymes in homogeneous cell preparation...

journal_title：Clinical biochemistry

authors： Casal JA,Cano E,Tutor JC

更新日期：2005-10-01 00:00:00

abstract：:We assessed the diagnostic value of four commercially available methods for determining pancreatic lipase (LPS) in serum (the turbidimetric procedure from Boehringer, two enzymatic approaches from Kodak and Poli, and an immunochemical assay) in a population of 46 hospitalized patients with acute abdominal pain. In 31 ...

journal_title：Clinical biochemistry

authors： Panteghini M,Pagani F,Bonora R,Alebardi O,Ceriotti F

更新日期：1991-12-01 00:00:00

abstract：OBJECTIVES:To develop a tandem mass spectrometry (MS/MS) method for plasma free and total carnitine, incorporating acid hydrolysis for total carnitine measurement, for improved accuracy and sample throughput. DESIGN AND METHODS:Limit of detection (LOD), limit of quantitation (LOQ), linearity, accuracy, precision coeff...

journal_title：Clinical biochemistry

authors： Johnson DW

更新日期：2010-11-01 00:00:00

abstract：OBJECTIVES:To compare HPLC methods with short and long elution times for HbA1c measurement in blood. METHODS:Comparison between G7-Tosoh (1.2 min); Bio-Rad-Variant-II-Turbo (1.3 min) and Arkray-HA-8160 (2.9 min). RESULTS:Passing-Bablok regression equations were: Y=0.17+0.96X; Y=-0.39+1.01X; Y=-0.40+1.0X and the means...

journal_title：Clinical biochemistry

authors： Leon-Justel A,Santotoribio JD,Dominguez-Pascual I,Delgado AL,Macias C,Herrera MT,Leal A,Mangas MA,Acosta D,Guerrero JM

更新日期：2009-10-01 00:00:00