Monoclonal antibody technology for cyclosporine monitoring.

Abstract:

:Monitoring blood levels of Cyclosporine (CsA) has been the basis for adjusting individual dosage regimens in the clinic. Radioimmunoassays using polyclonal antisera reacted with CsA and some CsA metabolites, leading to overestimation when compared with high-performance liquid chromatographic measurements of CsA. Monoclonal antibodies (mAbs) have the potential to discriminate between closely related molecules. MAbs with high affinity for CsA have been prepared and their fine-specificity characterized by cross-reactivity studies using a large series of CsA-derivatives. According to the known sites of metabolism on the CsA molecule and to its three-dimensional structure, it was possible to predict which mAb would be suitable for recognizing native Cs specifically.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Quesniaux VF

doi

10.1016/0009-9120(91)90147-7

subject

Has Abstract

pub_date

1991-02-01 00:00:00

pages

37-42

issue

1

eissn

0009-9120

issn

1873-2933

pii

0009-9120(91)90147-7

journal_volume

24

pub_type

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