An acid hydrolysis method for quantification of plasma free and total carnitine by flow injection tandem mass spectrometry.

Abstract:

OBJECTIVES:To develop a tandem mass spectrometry (MS/MS) method for plasma free and total carnitine, incorporating acid hydrolysis for total carnitine measurement, for improved accuracy and sample throughput. DESIGN AND METHODS:Limit of detection (LOD), limit of quantitation (LOQ), linearity, accuracy, precision coefficient of variation (CV), method comparison with a radioenzymatic assay (REA) and liquid chromatography tandem mass spectrometry assay (LC-MS/MS) were performed. The method was tested for interferences and for long-term performance. RESULTS:The method LOD and LOQ were 0.87 and 1.36 μmol/L for free carnitine and 1.79 and 2.54 μmol/L for total carnitine, respectively. No interferences were detected. Long-term measurement CVs for both free and total carnitine were <4%. CONCLUSIONS:The method shows acceptable performance characteristics with improved speed and low level accuracy compared to existing REA and MS/MS methods.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Johnson DW

doi

10.1016/j.clinbiochem.2010.08.015

subject

Has Abstract

pub_date

2010-11-01 00:00:00

pages

1362-7

issue

16-17

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(10)00374-7

journal_volume

43

pub_type

杂志文章
  • Plasma concentrations of Gas6 and soluble Axl correlate with disease and predict mortality in patients with critical limb ischemia.

    abstract:INTRODUCTION:Critical limb ischemia (CLI) is a severe peripheral arterial disease, characterized by rest pain, ulcers and gangrene in the legs. Gas6 is a vitamin K-dependent protein, which binds and activates the tyrosine kinase receptor Axl. Gas6-mediated Axl-signaling influences endothelial activation, neointima form...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2010.04.006

    authors: Ekman C,Gottsäter A,Lindblad B,Dahlbäck B

    更新日期:2010-07-01 00:00:00

  • Ethanolaminuria: a non-specific laboratory finding in the seriously ill infant.

    abstract::Ethanolamine is a compound that is frequently seen in urinary amino acid analysis. Although there is a single report of increased ethanolamine excretion associated with a distinctive storage disease in two siblings, the significance of ethanolaminuria is not known. We measured urinary ethanolamine/creatinine ratios in...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(88)80084-5

    authors: Cole DE,Farag S,Dooley KC

    更新日期:1988-10-01 00:00:00

  • Xanthine oxidase activity in patients with sepsis.

    abstract:OBJECTIVE:Determine the relation of xanthine oxidase (XO) activity and the outcome of septic patients and its relation to oxidative damage and clinical parameters of sepsis severity. DESIGN AND METHODS:Patients admitted over a 6-month period were enrolled. Patients were assigned to groups according to the diagnosis of...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2008.07.015

    authors: Luchtemberg MN,Petronilho F,Constantino L,Gelain DP,Andrades M,Ritter C,Moreira JC,Streck EL,Dal-Pizzol F

    更新日期:2008-10-01 00:00:00

  • mRNA levels of enzymes and receptors implicated in arachidonic acid metabolism in gliomas.

    abstract:BACKGROUND:Gliomas are tumors of the central nervous system derived from glial cells. They show cellular heterogeneity and lack specific diagnostic markers. Although a possible role for the eicosanoid cascade has been suggested in glioma tumorigenesis, the relationship between enzymes and receptors implicated in arachi...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2010.03.017

    authors: De Armas R,Durand K,Guillaudeau A,Weinbreck N,Robert S,Moreau JJ,Caire F,Acosta G,Pebet M,Chaunavel A,Marin B,Labrousse F,Denizot Y

    更新日期:2010-07-01 00:00:00

  • Soluble serum Klotho in diabetic nephropathy: relationship to VEGF-A.

    abstract:OBJECTIVES:Both kidney expression and soluble serum Klotho are influenced by chronic kidney disease (CKD) and diabetes. Serum Klotho is a yet poorly explored biomarker. We describe, for the first time to our knowledge, serum Klotho in diabetic patients with CKD and its relationship to vascular endothelial growth factor...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2012.07.098

    authors: Kacso IM,Bondor CI,Kacso G

    更新日期:2012-11-01 00:00:00

  • Establishment of reference intervals of 24 chemistries in apparently healthy adult Han population of Northern China.

    abstract:BACKGROUND:The availability of reference intervals is essential for physicians to interpret laboratory results. Most of our laboratory reference intervals are derived from data on the foreign population. We have studied the reference intervals of 24 common laboratory biochemical tests in an apparently healthy adult Han...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2012.06.022

    authors: Yang S,Qiao R,Li Z,Wu Y,Yao B,Wang H,Cui L,Yang Y,Zhang J

    更新日期:2012-10-01 00:00:00

  • Impact of long distance rowing on biological health: A pilot study.

    abstract:OBJECTIVES:To determine the impact of long distance rowing (160km, nonstop) on standard biological parameters and to study the relation between inflammation, myocardial necrosis, lipid profile, heart rate and energy expenditure. METHODS:Electrolytes, lipid profile, C-reactive protein (CRP), interleukin-6 (IL-6), tumor...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2017.11.018

    authors: Frias MA,Virzi J,Golaz O,Gencer B,Mach F,Vuilleumier N

    更新日期:2018-02-01 00:00:00

  • In vivo probes: problems and perspectives.

    abstract::Devices constructed for potential use as invasive bioprobes incorporate a selective receiving site for molecular or ionic recognition, and a transducer which is capable of translating a perturbation of physical chemistry of the determinant-site reaction (interaction) into a usable signal. Four types are envisioned--im...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/s0009-9120(86)80037-6

    authors: Thompson M,Vandenberg ET

    更新日期:1986-10-01 00:00:00

  • A decade of HPLC-MS/MS in the routine clinical laboratory--goals for further developments.

    abstract::During the past decade, tandem mass spectrometry hyphenated to liquid chromatography separation systems (HPLC-MS/MS) has developed to an important technology in clinical chemistry - not only for research purposes but also for routine use. At present, most important application fields are target analyses in therapeutic...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/j.clinbiochem.2008.02.017

    authors: Vogeser M,Seger C

    更新日期:2008-06-01 00:00:00

  • Usefulness of a new dialysis device adapted to small volume of red blood cells and its interest in epidemiology.

    abstract:OBJECTIVES:We checked the efficiency of a new dialysis device adapted to small volumes to remove glycerol from cryopreserved red blood cells. DESIGN AND METHODS:Dialysis was performed on D-Tube96™ Dialyzer Mini device. In a preliminary trial, we measured the residual glycerol before, and 2, 4 and 24 h after dialysis. ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2011.03.023

    authors: Zerimech F,Huyvaert H,Matran R,Nadif R

    更新日期:2011-06-01 00:00:00

  • Diagnostic and prognostic significance of human kallikrein 11 (KLK11) mRNA expression levels in patients with laryngeal cancer.

    abstract:OBJECTIVES:Human kallikrein 11 gene (KLK11) encodes a secreted serine protease. In view of its diagnostic and prognostic strength in many malignancies, we investigated the mRNA expression levels of KLK11 in laryngeal tissues in order to unveil its clinical usefulness in laryngeal cancer. DESIGN AND METHODS:KLK11 expre...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2012.03.005

    authors: Patsis C,Yiotakis I,Scorilas A

    更新日期:2012-06-01 00:00:00

  • Vitamins D and A can be successfully measured by LC-MS/MS in cord blood diluted plasma.

    abstract:OBJECTIVES:In widely used protocols for the collection and isolation of cord blood mononuclear cells, investigators are left with substantial volumes of diluted plasma which could be used for other measurements. The aim of this study was to ascertain the validity of umbilical cord blood (UCB) diluted plasma samples for...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2015.04.014

    authors: Albarhani AA,Collier F,Greaves RF,Ponsonby AL,Allen KJ,Vuillermin PJ,Roche P,Clarke MW,BIS Steering Committee.

    更新日期:2015-11-01 00:00:00

  • A sensitive and rapid mass spectrometric method for the simultaneous measurement of eight steroid hormones and CALIPER pediatric reference intervals.

    abstract:OBJECTIVES:To develop an accurate assay and establish the normal reference intervals for serum cortisol, corticosterone, 11-deoxycortisol, androstenedione, 21-hydroxyprogesterone, testosterone, 17-hydroxyprogesterone, and progesterone. These steroids are commonly used as biomarkers for the diagnosis and monitoring of e...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2013.01.002

    authors: Kyriakopoulou L,Yazdanpanah M,Colantonio DA,Chan MK,Daly CH,Adeli K

    更新日期:2013-05-01 00:00:00

  • Differential expression profiling of microRNAs and their potential involvement in renal cell carcinoma pathogenesis.

    abstract:OBJECTIVE:We seek to identify the differentially expressed miRNAs in the clear cell subtype (ccRCC) of kidney cancer. DESIGN AND METHODS:We performed a miRNA microarray analysis to compare the miRNA expression levels between ccRCC tissues and their normal counterpart. The top dysregulated miRNAs were validated by quan...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2009.07.020

    authors: Chow TF,Youssef YM,Lianidou E,Romaschin AD,Honey RJ,Stewart R,Pace KT,Yousef GM

    更新日期:2010-01-01 00:00:00

  • Phospholipase A2 group XV activity during cardiopulmonary bypass surgery.

    abstract:OBJECTIVES:All patients who undergo cardiopulmonary bypass (CPB) experience some degree of ischemia reperfusion injury (IRI). Severe IRI-induced acute kidney injury (AKI) occurs in approximately 1-2% of patients undergoing CPB. Previous studies using activity-based protein profiling of urine identified group XV phospho...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2020.12.003

    authors: Navarrete M,Wilkins JA,Chan FV,Ye B,Nickerson P,Ho J

    更新日期:2021-02-01 00:00:00

  • Biobanking 3.0: evidence based and customer focused biobanking.

    abstract::Biobanking is a new and very dynamic field. To achieve long term financial sustainability of biobank infrastructures we propose that a new focus is needed on activities, products and services provided by the biobank that relate to the external stakeholder: biobanking 3.0. Earlier stages of biobanking are biobanking 1....

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2013.12.018

    authors: Simeon-Dubach D,Watson P

    更新日期:2014-03-01 00:00:00

  • Urinary matrix metalloproteinase-8 and -9 activities in type 2 diabetic subjects: A marker of incipient diabetic nephropathy?

    abstract::Matrix metalloproteinases (MMPs) may play a pathophysiological role in the development of diabetic nephropathy (DN). We hypothesized that urinary MMP activity in patients with type 2 diabetes mellitus (T2DM) is related to a decline in renal function. We determined MMP-2, -8 and -9 activity in 24-h urine collections in...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2010.02.006

    authors: van der Zijl NJ,Hanemaaijer R,Tushuizen ME,Schindhelm RK,Boerop J,Rustemeijer C,Bilo HJ,Verheijen JH,Diamant M

    更新日期:2010-05-01 00:00:00

  • A simple matrix of analytical performance to identify assays that risk patients using External Quality Assurance Program data.

    abstract:OBJECTIVES:We propose a simple way to reliably rank assays for improvement according to patient risk, based solely on EQA imprecision and biological variation data. Because the underlying technique aligns the imprecision class of an assay from EQA data, peer performance can be used to assess achievable imprecision and ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2016.01.014

    authors: Mackay M,Hegedus G,Badrick T

    更新日期:2016-05-01 00:00:00

  • Lipid peroxidation and antioxidant system in the blood of patients with Hodgkin's disease.

    abstract:OBJECTIVES:The purpose of this study was to measure the extent of lipid peroxidation and the status of antioxidants in patients with Hodgkin's disease. DESIGN AND METHODS:Glutathione peroxidase (GPX) and superoxide dismutase (SOD) activities, and malondialdehyde (MDA), selenium, zinc and copper content have been measu...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(00)00062-x

    authors: Güven M,Oztürk B,Sayal A,Ozet A

    更新日期:2000-04-01 00:00:00

  • Water bath hyperthermia reduces stemness of colon cancer cells.

    abstract:OBJECTIVES:Researchers have proposed that cancer stem cells (CSCs) are the driving force of tumorigenesis, recurrence, and metastases, contributing to the failure of some cancer treatments. Water bath hyperthermia, a novel treatment for cancer, can be highly effective when combined with chemotherapy, radiotherapy, or i...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2013.08.023

    authors: Gao F,Ye Y,Zhang Y,Yang J

    更新日期:2013-11-01 00:00:00

  • Cell-free DNA in maternal plasma and serum: A comparison of quantity, quality and tissue origin using genomic and epigenomic approaches.

    abstract:OBJECTIVES:The objectives of this study were to compare the concentrations, size profiles and major tissue contributors of cell-free DNA (cfDNA) in plasma and in serum. DESIGN AND METHODS:Thirteen pregnant women in the third trimester were recruited for this study. We collected EDTA-plasma and serum samples using vari...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2016.09.009

    authors: Wong FC,Sun K,Jiang P,Cheng YK,Chan KC,Leung TY,Chiu RW,Lo YM

    更新日期:2016-12-01 00:00:00

  • Low normal thyroid function as a determinant of increased large very low density lipoprotein particles.

    abstract:OBJECTIVES:Low-normal thyroid function may relate to increases in plasma cholesterol and triglycerides, but effects on lipoprotein subfractions are largely unknown. Associations of alterations in lipoprotein metabolism and functionality with low-normal thyroid function could be more pronounced in Type 2 diabetes mellit...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2015.01.015

    authors: van Tienhoven-Wind L,Dullaart RP

    更新日期:2015-05-01 00:00:00

  • Relevance of pre-analytical blood management on the emerging cardiovascular protein biomarkers TWEAK and HMGB1 and on miRNA serum and plasma profiling.

    abstract:BACKGROUND:Disease-independent sources of biomarker variability include pre-analytical, analytical and biological variance. The aim of the present study was to evaluate whether the pre-analytical phase has any impact on the emerging heart disease TWEAK and HMGB1 protein markers and miRNA biomarkers, and whether peptido...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2016.11.005

    authors: Basso D,Padoan A,Laufer T,Aneloni V,Moz S,Schroers H,Pelloso M,Saiz A,Krapp M,Fogar P,Cornoldi P,Zambon CF,Rossi E,La Malfa M,Marotti A,Brefort T,Weis TM,Katus HA,Plebani M

    更新日期:2017-03-01 00:00:00

  • Comparison of gas-liquid chromatography and EMIT assay for serum valproic acid.

    abstract::We describe a simple direct extraction method for the gas-liquid chromatography determination of serum valproic acid. The working range for the assay is 2-180 mg/L and our within-run precision was 5.8 and 4.3% at the 40 and 90 mg/L concentrations respectively. Hemolyzed and lipemic sera as well as samples from patient...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(81)90772-4

    authors: Leroux M,Budnik D,Hall K,Irvine-Meek J,Otten N,Seshia S

    更新日期:1981-04-01 00:00:00

  • Plasma neutrophil gelatinase-associated lipocalin as a biomarker for acute kidney injury in critically ill patients with suspected sepsis.

    abstract:OBJECTIVES:The aim of this study was to investigate the diagnostic utility of plasma neutrophil gelatinase-associated lipocalin (NGAL) as an early objective biomarker to predict acute kidney injury (AKI) in critically ill patients with suspected sepsis, for whom procalcitonin (PCT) was used for the diagnosis and stagin...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2013.05.069

    authors: Kim H,Hur M,Cruz DN,Moon HW,Yun YM

    更新日期:2013-10-01 00:00:00

  • Toxic risks of inappropriate therapy.

    abstract::Drug therapy may produce toxicity. Patient individuality or drug interactions account for many cases of poisoning, but other factors such as genetic predisposition, drug contamination or human error are also known causes. Examples of various types of drug poisoning are given, illustrating the role of the clinical chem...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/0009-9120(90)90507-q

    authors: Stewart MJ

    更新日期:1990-02-01 00:00:00

  • Evaluation and assessment of high sensitivity thyrotropin methods as an index for thyroid function.

    abstract::We report the evaluation of four new commercially available sensitive assay kits for determination of thyrotropin (TSH) and their clinical utility in normal subjects and patients with thyroidal and non-thyroidal illnesses. The sensitivity for the reliable detection of serum TSH by these methods ranged from 0.1-0.4 mU/...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(87)80077-2

    authors: Kalra J,Laxdal VA,Walker SD,Massey KL

    更新日期:1987-10-01 00:00:00

  • The production of creatine kinase isozyme BB in sera of a patient with prostatic carcinoma and in tumor homogenates.

    abstract::1. A CK(BB) fraction was observed in sera of a patient with prostatic carcinoma. The fraction accounted for 59% of the total enzyme activity. A metastatic tumor of a prostatic carcinoma was observed to contain solely the CK(BB) isozyme. An embryonal testicular carcinoma was observed to have a higher percentage of the ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(78)90641-0

    authors: Hoag G,Amies DR,Colquhoun BD

    更新日期:1978-04-01 00:00:00

  • Characterization of a novel isolated deletion of the exon 3 within the CFTR gene: Relevance for phenotypic expression and genetic counseling.

    abstract:OBJECTIVES:To characterize a novel deletion of exon 3 of CFTR gene and to evaluate the implications in Cystic Fibrosis (CF) care and genetic counseling. DESIGN AND METHODS:We performed a wide mutational analysis of CFTR gene, using reverse dot blot, Multiplex Ligation-dependent Probe Amplification (MLPA) assay and Rea...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2011.03.140

    authors: Tomaiuolo AC,Sirleto P,Centrone C,Surace C,Alghisi F,Petrocchi S,Lombardo A,Rossi M,Torricelli F,Lucidi V,Angioni A

    更新日期:2011-07-01 00:00:00

  • Recent developments in meconium and hair testing methods for the confirmation of gestational exposures to alcohol and tobacco smoke.

    abstract::The use of alcohol and tobacco is prevalent among pregnant women despite the well-known adverse effects of these substances imposed on the developing fetus and immense public health education efforts. Confirmation of gestational exposures to these compounds have relied mostly on maternal self-reporting, which is often...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/j.clinbiochem.2004.01.010

    authors: Chan D,Caprara D,Blanchette P,Klein J,Koren G

    更新日期:2004-06-01 00:00:00