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Influence of temperature and period of freezing on the generation of hemolysate and blood cell lysate.

Abstract：

BACKGROUND:There is little information on temperature and period of freezing for generating hemolysate and blood cell lysate. MATERIALS AND METHODS:Primary tubes containing whole blood were frozen at -20°C and -80°C and then serially thawed to assess cell lysis and clinical chemistry tests in centrifuged plasma. RESULTS AND CONCLUSIONS:Massive amount of blood cells lysis could only be obtained by storing samples 12h at -20°C, or 2h at -80°C.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Lippi G,Musa R,Aloe R,Mercadanti M,Pipitone S

doi

10.1016/j.clinbiochem.2011.07.015

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

1267-9

issue

14-15

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(11)01429-9

journal_volume

44

pub_type

杂志文章

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