Platelet activating factor (PAF) and activity of its biosynthetic and catabolic enzymes in blood and leukocytes of male patients with newly diagnosed heart failure.


OBJECTIVES:To evaluate platelet activating factor (PAF) levels, its metabolic enzymes activity and its associations with other inflammatory markers in heart failure (HF) patients. DESIGN AND METHODS:PAF, and two of its key biosynthetic enzymes [lyso-PAF acetyltransferase (lyso-PAF-AT) and DTT-insensitive CDP-choline:1-alkyl-2-acetyl-sn-glycerol cholinephosphotransferase (PAF-CPT)] along with its catabolic enzymes [PAF-acetylhydrolase (PAF-AH) and lipoprotein-associated phospholipase-A(2) (Lp-PLA(2))] were measured in serum and leukocytes of twelve newly diagnosed male HF patients. Serum CRP, TNF-alpha, IL-6, sCD14 and CD40L were also determined. RESULTS:PAF ranged from 0.03 to 5.6 pmol/mL. Median lyso-PAF-AT, PAF-CPT, PAF-AH and Lp-PLA(2) activities were 4.1, 68.42, 644.44 pmol/min/mg protein and 51.42 pmol/min/microL correspondingly. Lyso-PAF-AT and PAF-CPT activities positively correlated with CRP, IL-6 and with each other, whereas PAF-CPT activity correlated with sCD14 and CD40L (P<0.05). CONCLUSIONS:PAF's biosynthetic enzyme activities correlated with inflammatory and immunologic molecules, which are activated in HF. Our study indicates a potential role of PAF in HF patients.


Clin Biochem


Clinical biochemistry


Detopoulou P,Nomikos T,Fragopoulou E,Antonopoulou S,Kotroyiannis I,Vassiliadou C,Panagiotakos DB,Chrysohoou C,Pitsavos C,Stefanadis C




Has Abstract


2009-01-01 00:00:00














  • An immunoassay for qualitative estimation of collagenase activity in mammalian tissues.

    abstract::Collagen, the most abundant protein of the mammalian body, is specifically degraded by collagenase. Collagenase activity and subsequent collagen degradation are the main aspects of essential biological processes such as bone remodeling, tissue repair and wound healing. Measurement of collagenase activity is of interes...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Chakraborty A,Eghbali M

    更新日期:1991-12-01 00:00:00

  • Knee replacement surgery significantly elevates the urinary inflammatory biomarkers neopterin and 7,8-dihydroneopterin.

    abstract:CONTEXT:Knee arthroplasty surgery is significant trauma, leading to an activated immune system causing inflammation and oxidative stress. Many current biomarkers are invasive, costly, and often slow to analyse, limiting their use for rapid inflammatory measurements. OBJECTIVES:We have examined the use of urinary neopt...

    journal_title:Clinical biochemistry

    pub_type: 临床试验,杂志文章,多中心研究


    authors: Baxter-Parker G,Roffe L,Cross S,Frampton C,Hooper GJ,Gieseg SP

    更新日期:2019-01-01 00:00:00

  • Can fecal calprotectin serve as a screen for necrotizing enterocolitis in infants?

    abstract:BACKGROUND:Researchers are debating if fecal calprotectin results are useful for infant patients, especially in screening for necrotizing enterocolitis (NEC). Currently, none of the FDA-approved calprotectin assays provide a cut-off for infant patients. We retrospectively analyzed data from a reference laboratory and u...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Goold E,Pearson L,Johnson LM

    更新日期:2020-10-01 00:00:00

  • Xanthine oxidase activity in patients with sepsis.

    abstract:OBJECTIVE:Determine the relation of xanthine oxidase (XO) activity and the outcome of septic patients and its relation to oxidative damage and clinical parameters of sepsis severity. DESIGN AND METHODS:Patients admitted over a 6-month period were enrolled. Patients were assigned to groups according to the diagnosis of...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Luchtemberg MN,Petronilho F,Constantino L,Gelain DP,Andrades M,Ritter C,Moreira JC,Streck EL,Dal-Pizzol F

    更新日期:2008-10-01 00:00:00

  • The biochemistry of mammalian senescence.

    abstract::Senescence is a process which, until quite recently, has been the subject of little scientific investigation. Even the word "senescence" is difficult to define, and complex methodological pitfalls have impeded progress. In the past few years, there have been exciting advances in understanding the physiological, cell b...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审


    authors: Kirkland JL

    更新日期:1992-04-01 00:00:00

  • The impact of time of sample collection on the measurement of thyroid stimulating hormone values in the serum.

    abstract:OBJECTIVE:The aim of our research is to determine whether the time of blood sampling and fasting of patients have an impact on TSH values. DESIGN AND METHODS:A total of 198 participants were enrolled in this study and classified into five groups: A--the first sample collection for TSH measurement was taken between 7:0...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Mirjanic-Azaric B,Stojakovic-Jelisavac T,Vukovic B,Stojanovic D,Vujnic M,Uletilovic S

    更新日期:2015-12-01 00:00:00

  • Antioxidant status, lipid peroxidation and nitric oxide end products in patients of type 2 diabetes mellitus with nephropathy.

    abstract:OBJECTIVES:Oxidative stress is considered to be a unifying link between diabetes mellitus (DM) and its complications including nephropathy. The aim of the present study was to evaluate oxidative stress status in Asian Indian patients of type 2 DM with nephropathy. DESIGN AND METHODS:Serum levels of malondialdehyde (MD...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Bhatia S,Shukla R,Venkata Madhu S,Kaur Gambhir J,Madhava Prabhu K

    更新日期:2003-10-01 00:00:00

  • Fecal chymotrypsin: a new diagnostic test for exocrine pancreatic insufficiency in children with cystic fibrosis.

    abstract::The purpose of this report is to evaluate whether a new, simple, non-invasive method for chymotrypsin measurement in stools is useful for the diagnosis of exocrine pancreatic insufficiency in cystic fibrosis (CF). A hundred children aged from 2 months to 12 years were tested: 50 children had been admitted for chronic ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Scotta MS,Marzani MD,Maggiore G,De Giacomo C,Melzi D'Eril GV,Moratti R

    更新日期:1985-08-01 00:00:00

  • Pitfalls to avoid when comparing CK-MB fractionation by antibody inhibition reaction and DEAE-Sephadex column chromatography.

    abstract::1. We compare the Roche ion-exchange column chromatography and the Merck antibody inhibition reaction for CK-MB fractionation in 51 sera. Measurements of total CK and CK-MB activities must be done under the same conditions for each method in order to correlate the results. 2. A decisional value must be used for the in...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Desjarlais F,Daigneault R

    更新日期:1979-08-01 00:00:00

  • Undetectable AMH at 3 days of age: a clue to bilateral anorchia.

    abstract::We report the case of a newborn boy who presented with micropenis and bilateral cryptorchidism at birth. The determination of AMH level strongly suggested a diagnosis of bilateral anorchia at 3 days of age whereas FSH and LH levels at the same time were not informative. The rapidity and reliability of results can redu...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Stoppa-Vaucher S,Djemli A,Van Vliet G

    更新日期:2010-11-01 00:00:00

  • Vitamin D-binding protein gene polymorphism association with IA-2 autoantibodies in type 1 diabetes.

    abstract:BACKGROUND:Vitamin D-binding protein (DBP) is the main systemic transporter of 1.25(OH)2D3 and is essential for its cellular endocytosis. There are two known polymorphisms in exon 11 of the DBP gene resulting in amino acid variants: GAT-->GAG substitution replaces aspartic acid by glutamic acid in codon 416; and ACG-->...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Ongagna JC,Pinget M,Belcourt A

    更新日期:2005-05-01 00:00:00

  • Comparative analysis of serum proteomes of patients with cardiovascular disease.

    abstract:OBJECTIVES:To monitor increases or decreases in cardiovascular disease (CVD)-related proteins that will be released from the deposits or plaque on the inner wall of blood vessels. DESIGN AND METHODS:Protein profiles of sera from healthy subjects and CVD patients were determined via 2-DE. Differentially expressed spots...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Kim HJ,Yoo HS,Kim PK,Kim MR,Lee HW,Kim CW

    更新日期:2011-02-01 00:00:00

  • Human bone cells contain a fluoride sensitive acid phosphatase: evidence that this enzyme functions at neutral pH as a phosphotyrosyl protein phosphatase.

    abstract::Fluoride is a potent therapeutic agent that increases spinal bone density in osteoporotic subjects. Based on work with animal cells previously, we proposed fluoride acts by inhibiting phosphotyrosyl protein phosphatase (EC activity in bone cells. The presence of fluoride sensitive acid phosphatase (EC 3.1.3....

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Wergedal JE,Lau KH

    更新日期:1992-02-01 00:00:00

  • Measurement of oxidized lipoprotein (a) in patients with acute coronary syndromes and stable coronary artery disease by 2 ELISAs: using different capture antibody against oxidized lipoprotein (a) or oxidized LDL.

    abstract:OBJECTIVE:To evaluate clinical value of oxidized lipoprotein(a) [ox-Lp(a)] levels. DESIGN AND METHODS:Ox-Lp(a) were measured by 2 ELISAs using antibodies against ox-Lp(a) [ox-Lp(a)1] or oxidized low-density lipoprotein [ox-Lp(a)2], and studied in 161 acute coronary syndromes (ACS) patients, 114 stable coronary artery ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Wang JJ,Han AZ,Meng Y,Gong JB,Zhang CN,Li K,Liu YX

    更新日期:2010-04-01 00:00:00

  • The possibility of determining N-acetyl-beta-D-glucosaminidase isoenzymes under alkaline conditions.

    abstract:OBJECTIVES:To have a reliable diagnostic test, the influence of urine pH on the determination of the total activity of N-acetyl-beta-D-glucosaminidase (NAG) and NAG isoenzyme activities was studied. DESIGN AND METHODS:After ultrafiltration and dialysis of the acidic and alkaline urines, the B, A, and A2 forms of NAG w...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Mandić LM,Aćimović JM,Jovanović VB

    更新日期:2005-04-01 00:00:00

  • The function, composition, and particle size of high-density lipoprotein were severely impaired in an oliguric phase of hemorrhagic fever with renal syndrome patients.

    abstract:BACKGROUND:Patients suffering from hemorrhagic fever with renal syndrome (HFRS) often showed strikingly reduced high-density lipoprotein (HDL)-cholesterol levels during the oliguric phase, indicating severe alterations in lipoprotein metabolism. OBJECTIVE:To compare changes in the functions and composition of HDL, lip...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Cho KH,Park SH,Park JE,Kim YO,Choi I,Kim JJ,Kim JR

    更新日期:2008-01-01 00:00:00

  • Long-term stability of biochemical markers in pediatric serum specimens stored at -80 °C: a CALIPER Substudy.

    abstract:OBJECTIVES:Pediatric serum samples collected from healthy children in the CALIPER (Canadian Laboratory Initiative in Pediatric Reference Interval) project are stored at -80 °C for various periods of time. This study aimed to determine the stability of chemistry, protein, and hormone analytes under these conditions. DE...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Brinc D,Chan MK,Venner AA,Pasic MD,Colantonio D,Kyriakopolou L,Adeli K

    更新日期:2012-07-01 00:00:00

  • Purification of three major forms of beta-hCG from urine and production of polyclonal antibodies against them.

    abstract:OBJECTIVES:This study has been conducted to develop a proper and relatively simple method for purification of three major forms of beta-hCG from urine to use as clinical calibrators and also to produce anti-beta-hCG polyclonal antibodies against these purified forms. DESIGN AND METHODS:Major beta-hCG forms from urine ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Khademi F,Hamzehee K,Mostafaie A,Hajihossaini R

    更新日期:2009-09-01 00:00:00

  • Analytical interference of 4-hydroxy-3-methoxymethamphetamine with the measurement of plasma free normetanephrine by ultra-high pressure liquid chromatography-tandem mass spectrometry.

    abstract:OBJECTIVES:The diagnosis of pheochromocytoma relies on the measurement of plasma free metanephrines assay whose reliability has been considerably improved by ultra-high pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Here we report an analytical interference occurring between 4-hydroxy-3-methoxym...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Dunand M,Donzelli M,Rickli A,Hysek CM,Liechti ME,Grouzmann E

    更新日期:2014-08-01 00:00:00

  • Problems with the estimation of urine protein by automated assays.

    abstract:OBJECTIVES:Most clinical laboratories replaced their manual precipitation techniques for the determination of urinary protein with automated dye binding assays or benzethonium chloride-turbidimetric assays. Few studies have validated these assays for the measurement of urinary proteins in the normal range. DESIGN AND ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Dube J,Girouard J,Leclerc P,Douville P

    更新日期:2005-05-01 00:00:00

  • A sensitive and rapid mass spectrometric method for the simultaneous measurement of eight steroid hormones and CALIPER pediatric reference intervals.

    abstract:OBJECTIVES:To develop an accurate assay and establish the normal reference intervals for serum cortisol, corticosterone, 11-deoxycortisol, androstenedione, 21-hydroxyprogesterone, testosterone, 17-hydroxyprogesterone, and progesterone. These steroids are commonly used as biomarkers for the diagnosis and monitoring of e...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Kyriakopoulou L,Yazdanpanah M,Colantonio DA,Chan MK,Daly CH,Adeli K

    更新日期:2013-05-01 00:00:00

  • An overview of the kallikrein gene families in humans and other species: emerging candidate tumour markers.

    abstract::Kallikreins are serine proteases with diverse physiologic functions. They are represented by multigene families in many animal species, especially in rat and mouse. Recently, the human kallikrein gene family has been fully characterized and includes 15 members, tandemly localized on chromosome 19q13.4. A new definitio...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审


    authors: Yousef GM,Diamandis EP

    更新日期:2003-09-01 00:00:00

  • Association between FSH, E1, and E2 levels in urine and serum in premenopausal and postmenopausal women.

    abstract:OBJECTIVE:We aimed to establish correlations for the levels of follicle-stimulating hormone (FSH), estrone (E1) and estradiol (E2) between urine and serum in premenopausal and postmenopausal women using immunoassays. METHODS:In this study of 92 women (61 postmenopausal, 31 premenopausal), both urine and blood specimen...

    journal_title:Clinical biochemistry

    pub_type: 临床试验,杂志文章


    authors: Onizuka Y,Nagai K,Ideno Y,Kitahara Y,Iwase A,Yasui T,Nakajima-Shimada J,Hayashi K

    更新日期:2019-11-01 00:00:00

  • Genetic risk factors for renal failure among north Indian ESRD patients.

    abstract:OBJECTIVES:Angiotensin converting enzyme (ACE), G-Protein couple receptor (G-Prot), endothelial nitric oxide synthase (ecNOS), Leptin -2548G/A and uncoupling protein (UCP2) are potent regulators of intra renal hemodynamics and may be the causative factors contributing to the deterioration of renal functions. In recent ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Tripathi G,Sharma RK,Baburaj VP,Sankhwar SN,Jafar T,Agrawal S

    更新日期:2008-05-01 00:00:00

  • Changes in serum enzyme activity after transcatheter arterial embolization for hepatic neoplasm.

    abstract::We assayed serum levels of certain enzymes and tumor markers in patients after transcatheter arterial embolization (TAE) to evaluate the effectiveness of this treatment. Twenty patients had hepatocellular carcinoma and two patients had metastases to the liver from colon cancer. Assays were first done immediately after...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Yoshikawa C,Shimojo N,Naka K,Akai T,Okuda K,Kaminou T,Yamada T,Nakatsuka H

    更新日期:1987-12-01 00:00:00

  • Quantitative analysis of trace elements in human blood and plasma by energy dispersive X-ray fluorescence.

    abstract::A photon excitation, secondary target x-ray fluorescence system was used to measure concentrations of Cu, Zn, Br and Rb in whole blood and plasma of healthy adults. The samples preparation method and calculated limits of detection of the technique are reported. Correlation statistics for elemental concentrations have ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Stump IG,Carruthers J,D'Auria JM

    更新日期:1977-06-01 00:00:00

  • Tentative identification of hemoglobin variants in the Bio-Rad VARIANT II Hb A1C method.

    abstract:OBJECTIVE:To evaluate if peak characteristics, the retention time, and percentage of total hemoglobin of an unknown peak on the Bio-Rad VARIANT II Hb A1c method may be used to establish a tentative identification of hemoglobin variants. METHOD:The peak characteristics, retention time, and percentage of total hemoglobi...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Higgins TN,Ridley B

    更新日期:2005-03-01 00:00:00

  • Elevated red blood cell distribution width at ICU discharge is associated with readmission to the intensive care unit.

    abstract:BACKGROUND:Red blood cell distribution width (RDW) is a predictor of mortality in critically ill patients. Our objective was to investigate the association between the RDW at ICU discharge and the risk of ICU readmission or unexpected death in the ward. METHODS:A secondary analysis of prospectively collected data stud...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: Tonietto TA,Boniatti MM,Lisboa TC,Viana MV,Dos Santos MC,Lincho CS,Pellegrini JAS,Vidart J,Neyeloff JL,Faulhaber GAM

    更新日期:2018-05-01 00:00:00

  • Clinical evaluation of a microsphere bead-based flow cytometry assay for the simultaneous determination of anti-thyroid peroxidase and anti-thyroglobulin antibodies.

    abstract:OBJECTIVES:Multiplexing technologies based on the use of microspheres as the solid phase have opened new possibilities for the analysis of autoantibodies. As an alternative to the traditional immunoassays, it is possible to use these methods in combination with flow cytometry for simultaneous measurement of anti-thyroi...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章


    authors: González C,García-Berrocal B,Talaván T,Casas ML,Navajo JA,González-Buitrago JM

    更新日期:2005-11-01 00:00:00

  • Interactions among endocrine control systems in the regulation of ovarian function.

    abstract::During sequential physiologic maturation of an individual follicle, the number of granulosa cells increases in excess of 1000-fold, while intra-ovarian concentrations of sex steroids escalate by 100-fold. The recent development of several in vitro ovarian systems has permitted a more extensive and direct assessment of...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审


    authors: Veldhuis JD

    更新日期:1981-10-01 00:00:00