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Plasma ADP levels: direct determination with luciferase luminescence using a biometer.

Abstract：

:A method is described for the determination of low plasma levels of adenosine-5'-diphosphate (ADP) using a Dupont Biometer to measure luminescence produced by the luciferin-luciferase reaction. Endogenous ATP is removed by incubation with luciferase. The remaining ADP is then quantitated, following its conversion to ATP, after incubation with creatine phosphate and creatine kinase. The mean coefficient of variation for 0.02 and 2.2 micromol/liter ADP standards were 2.1 and 1.8% respectively. The method has been applied to human and rabbit plasma. Human plasma ADP concentrations were found to be 0.13 +/- 0.025 (10) micromol/liter and rabbit plasma concentration were 0.07 +/- 0.05 (5) micromol/liter. Several other possible applications of the method are discussed.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Jabs CM,Ferrell WJ,Robb HJ

doi

10.1016/s0009-9120(78)80027-7

subject

Has Abstract

pub_date

1978-10-01 00:00:00

pages

190-3

issue

5

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(78)80027-7

journal_volume

11

pub_type

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