Abstract：
:The purpose of this report is to evaluate whether a new, simple, non-invasive method for chymotrypsin measurement in stools is useful for the diagnosis of exocrine pancreatic insufficiency in cystic fibrosis (CF). A hundred children aged from 2 months to 12 years were tested: 50 children had been admitted for chronic diarrhoea, 15 for cystic fibrosis and 40 acted as controls. Chymotrypsin in stools was assayed using a kinetic measurement with Succ-Ala-Ala-Pro-Phe-pNa as substrate in a simple photometric assay. In 13 of 15 children with cystic fibrosis, stool enzyme levels were always remarkably low, while all control subjects and all children not presenting cystic fibrosis had normal stool levels of chymotrypsin. Our data suggest that stool chymotrypsin measurement is a simple and reliable "tubeless" test for the evaluation of exocrine pancreatic insufficiency in children with cystic fibrosis.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Scotta MS,Marzani MD,Maggiore G,De Giacomo C,Melzi D'Eril GV,Moratti Rdoi
10.1016/s0009-9120(85)80046-1subject
Has Abstractpub_date
1985-08-01 00:00:00pages
233-4issue
4eissn
0009-9120issn
1873-2933pii
S0009-9120(85)80046-1journal_volume
18pub_type
杂志文章abstract：OBJECTIVES:The objectives of this study were to compare the concentrations, size profiles and major tissue contributors of cell-free DNA (cfDNA) in plasma and in serum. DESIGN AND METHODS:Thirteen pregnant women in the third trimester were recruited for this study. We collected EDTA-plasma and serum samples using vari...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2016.09.009
更新日期：2016-12-01 00:00:00
abstract：BACKGROUND:The accurate early diagnosis of acute kidney injury (AKI) in patients with acute heart failure (AHF) is an unmet clinical need. Cystatin C might improve the early detection of AKI. METHODS:207 patients presenting to the emergency department with AHF were enrolled. Cystatin C was measured in plasma in a blin...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2017.07.016
更新日期：2017-12-01 00:00:00
abstract：BACKGROUND:Niemann-Pick type C (NP-C) is a rare progressive neurodegenerative lipid storage disorder with heterogeneous clinical presentation and challenging diagnostic procedures. Recently oxysterols have been reported to be specific biomarkers for NP-C but knowledge on the intra-individual variation and on reference ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2015.03.007
更新日期：2015-06-01 00:00:00
abstract：OBJECTIVE:We used single-strand conformational polymorphism (SSCP) to screen for mutations at nucleotides 833 and 919 of the cystathionine beta-synthase (CBS) gene in 13 patients with homocystinuria and 11 of their relatives. METHODS:Exon 8 of genomic DNA was selectively amplified by PCR using primers derived from int...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(96)00045-8
更新日期：1996-10-01 00:00:00
abstract：:A commercially available assay (Sigma) for urinary oxalates based on the oxalate oxidase/peroxidase catalyzed reaction produced, in our hands, recoveries of added oxalate that varied from patient to patient. A modification of the assay is reported that involves the chromatographic separation of interfering substances,...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(90)80032-e
更新日期：1990-04-01 00:00:00
abstract：OBJECTIVES:This study explored the relationship between oxidative stress biomarkers and stability of carotid plaque. We decided to analyze the broad range of parameters describing oxidative stress in patients with carotid stenosis. DESIGN AND METHODS:124 consecutive patients undergoing carotid endarterectomy were enro...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2013.05.057
更新日期：2013-08-01 00:00:00
abstract：OBJECTIVES:To develop a simple and sensitive LC-MS/MS procedure for quantification of serum 25-OH-vitamin D₃ (25-OH-D₃), 25-OH-vitamin D₂ (25-OH-D₂), and their C3-epimers. METHODS:Serum 25-OH-vitamin D metabolites were extracted with MTBE and quantified by LC-MS/MS. Commercially available calibrators and QC materials ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2012.11.030
更新日期：2013-09-01 00:00:00
abstract：OBJECTIVES:Living donor kidney transplantation is regarded as beneficial to allograft recipients and not particularly detrimental to the donors. Recently we have documented a reduced glomerular filtration rate (GFR) in living kidney donors (LKDs). Considering kidneys as essential for l-arginine (Arg) metabolism, in the...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2013.04.031
更新日期：2013-10-01 00:00:00
abstract：OBJECTIVES:The fibroblast growth factor receptor 3 gene (FGFR3) plays a critical role in cartilage growth-plate differentiation and bony development. It has been shown that 97% of patients with achondroplasia have a G to A transition mutation at position 1138 (c.1138 G>A) of codon 380 of the FGFR3 gene. DESIGN AND MET...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2007.08.014
更新日期：2008-02-01 00:00:00
abstract：OBJECTIVES:To evaluate the effect of Matrix metalloproteinase 7 -181A/G polymorphism (rs11568818) on the risk of cancer, we performed a meta-analysis. DESIGN AND METHODS:24 case-control studies were identified by searching Pubmed, Embase, ISI, OVID MEDLINE and EBSCO databases. Odd ratios and corresponding 95% confiden...
journal_title：Clinical biochemistry
pub_type： 杂志文章,meta分析
doi：10.1016/j.clinbiochem.2013.07.015
更新日期：2013-11-01 00:00:00
abstract：BACKGROUND:Myocardial infarction (MI) is more likely if the heart damage biomarker cardiac troponin T (cTnT) is elevated in a blood sample from a patient with chest pain. There is no conventional method to estimate the risk of MI at a specific cTnT concentration. The purpose of this study was to evaluate the performanc...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2019.02.003
更新日期：2019-04-01 00:00:00
abstract：:A spectrophotometric study was carried out for three proteins assays when modification of their serum procedures using bromcresol green, bromcresol purple and biuret reagents were applied to the determinations of total proteins and albumin in cerebrospinal fluids. A novel concentration device wherein the sample itself...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(81)90165-x
更新日期：1981-02-01 00:00:00
abstract：OBJECTIVES:Matrix-assisted laser desorption time-of flight ionization (MALDI)-imaging MS (IMS) with MSMS analysis using on-tissue tryptic digests is a powerful tool for identification of disease-related proteins in formalin-fixed paraffin-embedded (FFPE) tissue sections. We applied this novel IMS technique, not only to...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2013.05.063
更新日期：2013-10-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to determine the adult reference values for cystatin C (CysC) and to evaluate their consistence with those reported in the literature. DESIGN AND METHODS:CysC was analyzed in a consecutive series of subjects (100 males and 100 females) by a nephelometric immunoassay. Medline was sea...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2007.05.011
更新日期：2007-09-01 00:00:00
abstract：OBJECTIVES:The glutathione S-transferases (GSTs) play a critical role in protecting the colorectal mucosa. We investigated the efficacy of using the GSTs activity of plasma as a biomarker of risk for colorectal cancer. METHODS:GSTs activity was measured in the plasma of control individuals (n=39) and in the plasma, tu...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2005.04.004
更新日期：2005-07-01 00:00:00
abstract：BACKGROUND:According to the prevailing theory about the genetic background to lactose intolerance, there are three genotypes but only two adult physiological phenotypes: lactase persistence in individuals with the CT and TT genotypes and lactase non-persistence in individuals with the CC genotype. However, analysis of ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2015.11.001
更新日期：2016-02-01 00:00:00
abstract：OBJECTIVES:The aim was to study the influence of sampling time, food intake and sleep on tests used to screen for and monitor conditions of iron deficiency and overload. DESIGN AND METHODS:The 24 h variations of iron, transferrin, transferrin saturation (TS) and ferritin were studied in seven healthy individuals durin...
journal_title：Clinical biochemistry
pub_type： 临床试验,杂志文章
doi：10.1016/j.clinbiochem.2010.08.023
更新日期：2010-11-01 00:00:00
abstract：OBJECTIVE:To evaluate a novel surface plasmon resonance (SPR) system for the direct measurement of tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva. DESIGN AND METHODS:We measured the presence of the tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva using 2 different surface plasmon resona...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2012.09.001
更新日期：2012-12-01 00:00:00
abstract：:1. When a malignant growth is developing in a patient, one often observes in the serum an increased activity of serum enzymes due to necrosis and increased membrane permeability caused by the altered metabolism of malignant cells. Conversely, I considered it interesting to check the enzymes of the malate-aspartate sh...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(77)92378-5
更新日期：1977-08-01 00:00:00
abstract：OBJECTIVES:This study aimed to examine the association between promoter polymorphisms of Th1 and Th2 cytokine genes [interleukin-4 (IL-4 T-34C, A-81G, C-285T and T-589C), IL-6 (G-174C), IL-10 (A-592C and T-819C) and tumour necrosis factor-alpha (TNF-alpha G-238A and G-308A)] and Graves' disease (GD) in Taiwanese popula...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2006.11.009
更新日期：2007-02-01 00:00:00
abstract：OBJECTIVES:To evaluate the results of oxygen consumption measurement in lymphocytes for the diagnosis and treatment monitoring of pediatric patients with oxidative phosphorylation diseases. DESIGN AND METHODS:Twenty-four children with an oxidative phosphorylation disease were studied. Results were compared with those ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(00)00137-5
更新日期：2000-08-01 00:00:00
abstract：OBJECTIVES:The relative proportion in percentage of the isoenzyme A of beta-hexosaminidase (Hex) is the single discriminatory function most frequently used for the biochemical screening of heterozygote Tay-Sachs disease carriers. It has been suggested that the assay of the Hex isoenzymes in homogeneous cell preparation...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2005.05.016
更新日期：2005-10-01 00:00:00
abstract：OBJECTIVE:To clarify the possibility that midband Lp in LDL fractions might act as an atherogenic lipoprotein in their interaction with macrophages. DESIGN AND METHODS:Low density lipoproteins (LDL) isolated by zonal ultracentrifugation from midband lipoprotein-positive serum in type lib hyperlipidemics were subjected...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(96)00009-c
更新日期：1996-06-01 00:00:00
abstract：OBJECTIVES:The diagnosis of pheochromocytoma relies on the measurement of plasma free metanephrines assay whose reliability has been considerably improved by ultra-high pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Here we report an analytical interference occurring between 4-hydroxy-3-methoxym...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2014.04.003
更新日期：2014-08-01 00:00:00
abstract：OBJECTIVE:To assess the generation of reactive oxygen species (ROS) and the involvement of the main antioxidant pathways in low/intermediate-1-risk myelodysplastic syndromes (MDS) with iron overload (IOL). METHODS:We examined the levels of superoxide anion (O2-), hydrogen peroxide (H2O2), antioxidants (glutathione, GS...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2017.05.018
更新日期：2017-11-01 00:00:00
abstract：:Only recently has radioimmunoassay been used for the detection of drugs of abuse in body fluids. The radioimmunoassay method is rapid, sensitive, specific, and can be performed with a minimum of sample, while conventional assay methods are time-consuming, relatively insensitive, and require a larger sample volume. Per...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(78)90098-x
更新日期：1978-06-01 00:00:00
abstract：BACKGROUND:Red blood cell distribution width (RDW) is a predictor of mortality in critically ill patients. Our objective was to investigate the association between the RDW at ICU discharge and the risk of ICU readmission or unexpected death in the ward. METHODS:A secondary analysis of prospectively collected data stud...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2018.03.010
更新日期：2018-05-01 00:00:00
abstract：OBJECTIVES:The haphazard distribution of fibrous tissue can interfere with quantitative methods for evaluating hepatic fibrosis. Inter-sample variation may represent a crucial issue when hydroxyproline measurement is used to quantify fibrosis. A comparative study of the hydroxyproline levels in normal and fibrotic rats...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2006.08.002
更新日期：2006-12-01 00:00:00
abstract：:The bromocresol purple (BCP) albumin method on the DuPont aca was evaluated by determining the accuracy and precision of the method. A split sample comparison was performed against the electroimmunoassay (EIA) method as well as the bromocresol green (BCG) method on the DuPont aca and the Technicon SMA 12/60. The BCP m...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(83)90490-3
更新日期：1983-04-01 00:00:00
abstract：:We report the case of a newborn boy who presented with micropenis and bilateral cryptorchidism at birth. The determination of AMH level strongly suggested a diagnosis of bilateral anorchia at 3 days of age whereas FSH and LH levels at the same time were not informative. The rapidity and reliability of results can redu...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2010.08.022
更新日期：2010-11-01 00:00:00
