Abstract：
OBJECTIVES:The haphazard distribution of fibrous tissue can interfere with quantitative methods for evaluating hepatic fibrosis. Inter-sample variation may represent a crucial issue when hydroxyproline measurement is used to quantify fibrosis. A comparative study of the hydroxyproline levels in normal and fibrotic rats is herein reported. MATERIAL AND METHODS:Twelve normal and 20 Capillaria hepatica-infected Wistar rats were used. Two fragments of the liver (A and B) of each rat were taken from separate areas and hydroxyproline measurements were made. Calculated differences in hydroxyproline measurements between samples from the same liver were analyzed by BOOTSTRAP. RESULTS:Differences in normal rats varied from 0.026 to 1.85 micromol of HP/g, in ten rats, the difference was less than 0.50 micromol. In infected rats, it varied from 0.04 to 2.86 micromol HP/g. Differences higher than 0.69 micromol/g were significant for normal rats (p<0.05) and above 1.22 micromol/g (p<0.05) for fibrotic rats. CONCLUSIONS:Hydroxyproline ratio in a normal liver kept a fair degree of reproducibility. In the presence of hepatic fibrosis, the levels of hydroxyproline may vary significantly between samples from a single liver and may have limited value in quantifying the extent of fibrosis.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Gomes AT,Bastos CG,Afonso CL,Medrado BF,Andrade ZAdoi
10.1016/j.clinbiochem.2006.08.002subject
Has Abstractpub_date
2006-12-01 00:00:00pages
1160-3issue
12eissn
0009-9120issn
1873-2933pii
S0009-9120(06)00254-2journal_volume
39pub_type
杂志文章abstract：INTRODUCTION:The clinical catchment area for the Metabolic service at the Women's and Children's Hospital in Adelaide, South Australia, covers nearly 2.5millionkm2. Care of children with metabolic disorders in these remote areas is assisted from Adelaide, and at times, using plasma ammonia results from laboratories up ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2016.11.025
更新日期：2017-04-01 00:00:00
abstract：OBJECTIVES:Coenzyme Q(10) (CoQ(10)) is an essential electron carrier in the mitochondrial respiratory chain and a strong antioxidant. Signs associated with skin alteration and mitochondrial dysfunction have been observed in patients with fibromyalgia (FM). The aim of this study was to analyze CoQ(10) levels, mitochondr...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2010.06.013
更新日期：2010-09-01 00:00:00
abstract：BACKGROUND:Genetic variations of UDP-glucuronyltransferase 1A1 (UGT1A1) influence the concentration of serum bilirubin. We investigated the association of four common polymorphisms including UGT1A1-53(TA)(n), and common haplotypes of the UGT1A1 gene with bilirubin levels in 218 Caucasian volunteers. METHODS:Total bili...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2009.08.011
更新日期：2009-11-01 00:00:00
abstract：OBJECTIVES:This study aimed to examine the association between promoter polymorphisms of Th1 and Th2 cytokine genes [interleukin-4 (IL-4 T-34C, A-81G, C-285T and T-589C), IL-6 (G-174C), IL-10 (A-592C and T-819C) and tumour necrosis factor-alpha (TNF-alpha G-238A and G-308A)] and Graves' disease (GD) in Taiwanese popula...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2006.11.009
更新日期：2007-02-01 00:00:00
abstract：OBJECTIVES:Abnormal alanine aminotransferase (ALT) activity is indicative of liver disease even a burden of overall health. We assessed the factors associated with ALT activity and their internal relationships in a male population from southern China. DESIGN AND METHODS:Data of physical examinations, laboratory tests,...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2012.08.022
更新日期：2012-12-01 00:00:00
abstract：OBJECTIVE:The relationship between the severity of atherosclerotic coronary artery disease (CAD) and circulating levels of salusin-α, salusin-β and heregulin-β1 has been investigated. In addition, the relationship with these peptides and high sensitive C-reactive protein (hsCRP) has been investigated. METHODS:The stud...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2020.10.003
更新日期：2021-01-01 00:00:00
abstract：OBJECTIVES:Drug screening in neonates is traditionally performed using meconium, but cord tissue has been proposed as an alternative specimen. This study compares the detection of drugs in a large number of paired meconium and umbilical cord tissue samples from subjects at risk of in utero drug exposure. DESIGN AND ME...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2017.03.006
更新日期：2017-09-01 00:00:00
abstract：OBJECTIVES:The objectives of this study were to compare the concentrations, size profiles and major tissue contributors of cell-free DNA (cfDNA) in plasma and in serum. DESIGN AND METHODS:Thirteen pregnant women in the third trimester were recruited for this study. We collected EDTA-plasma and serum samples using vari...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2016.09.009
更新日期：2016-12-01 00:00:00
abstract：:We present a systematic parametric method for resolving age-related reference intervals based on differential-polynomial regression analysis. Application of this method to plasma zinc concentrations in a childhood population results in a third degree polynomial expression from which two statistically independent age-r...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(79)90056-0
更新日期：1979-02-01 00:00:00
abstract：:Preparation of (14C) Pro labeled protocollagen and requirements for its hydroxylation by PPH was investigated. Protocollagen can be prepared by centrifugation of the biological material at 15.000 X g. Substances in current use, viz catalase, bovine serum albumin and dithiothreitol were found unnecessary under the hydr...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(76)80072-0
更新日期：1976-12-01 00:00:00
abstract：BACKGROUND:Elevations of serum cardiac troponin T (cTnT) have been described in patients with end stage chronic kidney disease (CKD) although the mechanism is unknown, whether from increased production or decreased clearance. Less is known about cTnT in short term changes in renal function and in lesser degrees of rena...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2015.05.004
更新日期：2015-08-01 00:00:00
abstract：OBJECTIVE:To establish a plasma calibration curve for the Advantage Blood Glucose System that would permit direct comparison with results provided by clinical laboratories. DESIGN AND METHODS:The initial study involved the comparison between the Advantage whole blood referenced results and the laboratory plasma result...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(97)00074-x
更新日期：1997-08-01 00:00:00
abstract：BACKGROUND:Plasma concentrations of human chorionic gonadotropin (hCG) have been shown to increase with age due to pituitary secretion. We previously recommended that an hCG cutoff of 14.0IU/L be used for women ≥55years of age. However, it remains unknown whether concentrations >14.0IU/L can be expected in women with a...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2016.11.017
更新日期：2017-03-01 00:00:00
abstract：OBJECTIVES:To investigate the association of parental hyperhomocysteinemia, C677T Methylene tetrahydrofolate reductase (MTHFR) polymorphism and DNA damage with recurrent pregnancy loss (RPL). DESIGN AND METHODS:A case-control study. Reverse phase HPLC, PCR-RFLP and Cytokinesis blocked micronuclei assay were used to as...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2008.12.003
更新日期：2009-03-01 00:00:00
abstract：:Steroids play an important role in sperm production and quality. These hormones have been extensively studied in blood, but poorly investigated in semen. The purpose of our study was to evaluate the relationship between sperm quality and steroid profiles in blood and semen in a small cohort of young Swiss men. Another...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2018.03.008
更新日期：2018-12-01 00:00:00
abstract：:Bone metabolism is assessed using biochemical bone turnover markers (BTM). BTM reflect the metabolic effect of drugs on bone turnover, help to establish the lowest dose inducing the largest change in the BTM, predict treatment-related reduction in fracture risk, and are helpful in bridging studies. Changes in BTM duri...
journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.clinbiochem.2012.01.022
更新日期：2012-08-01 00:00:00
abstract：OBJECTIVE:To review screening for cancer in high risk families. METHODS AND RESULTS:Screening for hereditary cancer involves three steps: it is first necessary to identify families at high risk by examining the number and sites of cancer in a family. Special attention is given to cancers appearing at an early age, to ...
journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/0009-9120(95)00022-2
更新日期：1995-08-01 00:00:00
abstract：:Drug therapy may produce toxicity. Patient individuality or drug interactions account for many cases of poisoning, but other factors such as genetic predisposition, drug contamination or human error are also known causes. Examples of various types of drug poisoning are given, illustrating the role of the clinical chem...
journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/0009-9120(90)90507-q
更新日期：1990-02-01 00:00:00
abstract：OBJECTIVES:Lipoprotein(a) [Lp(a)] can be oxidized by copper in vitro in a way comparable to low-density lipoprotein (LDL). We sought to determine whether the susceptibility of Lp(a) to oxidation is correlated with the susceptibility of autologous heterogeneous LDL, with apolipoprotein(a) [apo(a)] molecular size, or wit...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(02)00438-1
更新日期：2003-03-01 00:00:00
abstract：:The biosynthesis of prostate specific antigen (PSA) was studied in human epidermoid carcinoma, (KB) cells and in normal human embryonic lung (WI-38)cells. The prostate carcinoma cell line, PC-35F12 was used as a control. PSA specific antibodies were used to precipitate the immunologically reactive peptides from cell e...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(01)00280-6
更新日期：2001-11-01 00:00:00
abstract：OBJECTIVE:To review biochemical literature and determine the significance of non-covalent bonding in plasma. RESULTS:Many non-covalent bonds exist in plasma. Relatively strong self-associations are found for serum albumin, several gamma-globulins, apolipoproteins and fibrin(ogen). Here, fibrinogen and fibrin, consider...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(96)00159-2
更新日期：1997-03-01 00:00:00
abstract：OBJECTIVES:To test for specific mutations in the alanine:glyoxylate aminotransferase (AGT) gene, in order to diagnose primary hyperoxaluria type 1 (PH1). DESIGN AND METHODS:Samples of liver and/or DNA from 81 patients were submitted to our laboratory for diagnostic testing for PH1. Using a panel of selected mutations,...
journal_title：Clinical biochemistry
pub_type： 杂志文章,多中心研究
doi：10.1016/j.clinbiochem.2008.01.018
更新日期：2008-05-01 00:00:00
abstract：OBJECTIVES:Vitamin B(12), or B(12), is an essential nutrient for humans, and its deficiency is a public health problem, especially in elderly population. Around 30% of circulating total B(12) levels are attached to transcobalamin II (TCN2), being referred as holotranscobalamin (holo-TC), and representing the biological...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2010.01.015
更新日期：2010-05-01 00:00:00
abstract：BACKGROUND:The aldosterone/renin ratio (ARR) is recommended to screen for primary aldosteronism (PA) in hypertension. We estimated fully automated chemiluminescence immunoassays (CLIA) for plasma aldosterone concentrations (PAC) and plasma direct renin concentrations (PRC) and investigated their reference intervals in ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2018.04.016
更新日期：2018-06-01 00:00:00
abstract：OBJECTIVES:To compare the accuracy of procalcitonin (PCT) in early-onset neonatal sepsis (EOS) using standard cut-off values and a multilevel probabilistic approach. DESIGN AND METHODS:A retrospective study of PCT was performed in 149 newborns at risk of EOS, including preterm or prolonged rupture of membranes, chorio...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2008.05.015
更新日期：2008-10-01 00:00:00
abstract：OBJECTIVES:Measurement of serotonin concentrations in blood is considered to be important for the clinical studies of diseases involving vascular endothelial injury. We have developed a high-performance liquid chromatographic (HPLC) method for measurement of serotonin level in platelet-poor plasma (PPP). METHODS:Venou...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2003.11.009
更新日期：2004-03-01 00:00:00
abstract：OBJECTIVES:The present study was designed to (a) evaluate and compare plasma ammonia levels (PAL) in patients with acute liver failure (ALF) and chronic liver disease (CLD) with or without hepatic encephalopathy (HE); (b) correlate the severity of HE with PAL; and (c) correlate PAL with clinical features of raised intr...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2005.04.013
更新日期：2005-08-01 00:00:00
abstract：:A procedure for the assay of glucose-6-phosphate dehydrogenase (EC 1.1.1.49) in erythrocytes on the GEMSAEC centrifugal analyzer using a modification of Beutler's procedure is described. Glucose-6-phosphate dehydrogenase activity is corrected for the contributing activity of 6-phosphogluconate dehydrogenase (EC 1.1.1....
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(79)80085-5
更新日期：1979-10-01 00:00:00
abstract：:A method is described for the determination of low plasma levels of adenosine-5'-diphosphate (ADP) using a Dupont Biometer to measure luminescence produced by the luciferin-luciferase reaction. Endogenous ATP is removed by incubation with luciferase. The remaining ADP is then quantitated, following its conversion to A...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(78)80027-7
更新日期：1978-10-01 00:00:00
abstract：OBJECTIVE:Can biochip arrays identify which individuals with metastatic disease will respond to an anti-metastatic agent? DESIGN AND METHODS:Cytokine and cell adhesion arrays (Randox Ltd) were measured over 1 month in 9 research participants receiving CTCE-9908 in a Phase I/II study. RESULTS:Research participants wit...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2009.04.008
更新日期：2009-07-01 00:00:00
