Simplified method for determination of protocollagen proline hydroxylase.

abstract：OBJECTIVE:To establish a plasma calibration curve for the Advantage Blood Glucose System that would permit direct comparison with results provided by clinical laboratories. DESIGN AND METHODS:The initial study involved the comparison between the Advantage whole blood referenced results and the laboratory plasma result...

journal_title：Clinical biochemistry

authors： Lane A,Geadah D,Laferriere M,Lebrun M,Caron D,Ross P

更新日期：1997-08-01 00:00:00

abstract：:Ischemic heart disease is the primary cause of death worldwide. The pathophysiological process of cardiovascular diseases is linked to atheromatous plaque formation, while plaque rupture releases thrombogenic elements, which lead to activation of platelets, blood clotting and formation of thrombi. Platelet inhibitors ...

journal_title：Clinical biochemistry

authors： Nanau RM,Delzor F,Neuman MG

更新日期：2014-05-01 00:00:00

abstract：OBJECTIVES:To investigate the association of parental hyperhomocysteinemia, C677T Methylene tetrahydrofolate reductase (MTHFR) polymorphism and DNA damage with recurrent pregnancy loss (RPL). DESIGN AND METHODS:A case-control study. Reverse phase HPLC, PCR-RFLP and Cytokinesis blocked micronuclei assay were used to as...

journal_title：Clinical biochemistry

authors： Govindaiah V,Naushad SM,Prabhakara K,Krishna PC,Radha Rama Devi A

更新日期：2009-03-01 00:00:00

abstract：OBJECTIVES:The aim of the study was to evaluate the concentration of interleukin 12 (IL-12), the activity of phospholipase A(2) (PLA(2)), and platelet-activating factor acetylhydrolase (PAF-AH) in type 1 diabetes (DM1) patients treated with intensive insulin therapy. DESIGN AND METHODS:Studied parameters were measured...

journal_title：Clinical biochemistry

authors： Wegner M,Araszkiewicz A,Pioruńska-Mikołajczak A,Zozulińska-Ziółkiewicz D,Wierusz-Wysocka B,Pioruńska-Stolzmann M

更新日期：2009-11-01 00:00:00

abstract：OBJECTIVES:Increased free radical activity and lipid peroxidation may be implicated in the pathogenesis of preeclampsia. This study was initiated to assess antioxidant enzyme and trace metals's status in preeclampsia. DESIGN AND METHODS:The comparison was made between the pregnant women with or without preeclampsia an...

journal_title：Clinical biochemistry

authors： Ilhan N,Ilhan N,Simsek M

更新日期：2002-07-01 00:00:00

abstract：OBJECTIVES:Studies from both experimental animals and humans suggest that administration of exogenous DHEA may have beneficial endocrine-metabolic, immunologic and neurologic effects. Several groups have administered DHEA to humans, but to the best of our knowledge, no one at this point has published a summary of the r...

journal_title：Clinical biochemistry

authors： Tummala S,Svec F

更新日期：1999-07-01 00:00:00

abstract：OBJECTIVES:Two methods, Petersen's error grid analysis and Shermock's method to detect clinically important differences, were recently developed to advance the assessment of analytic performance of point-of-care INR devices. Both methods predict when alternate INR measures lead to different clinical decisions. Our goal...

journal_title：Clinical biochemistry

authors： Shermock KM,Tandon S,Sorgen PJ,Lavallee DC,Clarke W,Streiff MB

更新日期：2012-05-01 00:00:00

abstract：:Mycophenolate mofetil is widely used in combination with either cyclosporine or tacrolimus for rejection prophylaxis in renal and heart transplant patients. Although not monitored routinely nearly to the degree that other agents such as cyclosporine or tacrolimus, there is an expanding body of experimental evidence fo...

journal_title：Clinical biochemistry

authors： Shaw LM,Korecka M,DeNofrio D,Brayman KL

更新日期：2001-02-01 00:00:00

abstract：OBJECTIVES:We studied the stability of maternal blood markers for screening for Down syndrome (alpha-fetoprotein, unconjugated estriol, intact human chorionic gonadotropin (hCG) and free beta-human chorionic gonadotropin) upon repeated freeze-thaw cycles. DESIGN AND METHODS:Forty-three samples collected from second tr...

journal_title：Clinical biochemistry

authors： Massé J,Forest JC,Moutquin JM,Degrandpre P

更新日期：1997-10-01 00:00:00

abstract：OBJECTIVES:This study was undertaken to evaluate the relationship between serum bilirubin concentrations and the degree of urinary albumin excretion in hypertensive patients. DESIGN AND METHODS:A total of 120 hypertensive subjects were enrolled, in which 80 (67%) with normoalbuminuria (albumin excretion rate [AER] of ...

journal_title：Clinical biochemistry

authors： Huang SS,Huang PH,Chiang KH,Chen JW,Lin SJ

更新日期：2011-07-01 00:00:00

abstract：OBJECTIVES:Although numerous recent studies have focused on analyses of microRNA expression profiles in cancer cells, the expression patterns of the enzymes responsible for the generation of miRNAs remains largely unexplored. The purpose of this study was to investigate whether Dicer mRNA expression is altered during p...

journal_title：Clinical biochemistry

authors： Pampalakis G,Diamandis EP,Katsaros D,Sotiropoulou G

更新日期：2010-02-01 00:00:00

abstract：:We evaluated a method for quantifying bone isoenzyme of alkaline phosphatase (ALP) which utilizes wheat-germ lectin to precipitate this fraction. In precision studies, CVs ranged from 3.2 to 11.4% (within-day) and from 3.7 to 11.5% (day-to-day). The assay procedure was linear to 1100 U/L and was easily adapted to auto...

journal_title：Clinical biochemistry

authors： Burlina A,Plebani M,Secchiero S,Zaninotto M,Sciacovelli L

更新日期：1991-10-01 00:00:00

abstract：BACKGROUND:Vitamin D-binding protein (DBP) is the main systemic transporter of 1.25(OH)2D3 and is essential for its cellular endocytosis. There are two known polymorphisms in exon 11 of the DBP gene resulting in amino acid variants: GAT-->GAG substitution replaces aspartic acid by glutamic acid in codon 416; and ACG-->...

journal_title：Clinical biochemistry

authors： Ongagna JC,Pinget M,Belcourt A

更新日期：2005-05-01 00:00:00

abstract：UNLABELLED:Antioxidant capacity testing is commonly used in clinical investigations to provide an estimate of in vitro antioxidant capacity of biosamples. Although beneficial to measure the synergistic contribution of all compounds with antioxidant functionality, assessing the capacity of non-protein fractions or small...

journal_title：Clinical biochemistry

authors： Crowe KM

更新日期：2014-10-01 00:00:00

abstract：:Technological developments and economic drivers have led to major changes in clinical laboratories worldwide, principally represented by an increase in testing productivity and efficiency. Currently, major expectations are entrusted on the introduction of diagnostically more effective tests as well as on translating t...

journal_title：Clinical biochemistry

authors： Plebani M,Lippi G

更新日期：2010-08-01 00:00:00

abstract：:We present a systematic parametric method for resolving age-related reference intervals based on differential-polynomial regression analysis. Application of this method to plasma zinc concentrations in a childhood population results in a third degree polynomial expression from which two statistically independent age-r...

journal_title：Clinical biochemistry

authors： Butrimovitz GP,Purdy WC

更新日期：1979-02-01 00:00:00

abstract：AIM:Medical laboratories are the key partners in patient safety. Laboratory results influence 70% of medical diagnoses. Quality of laboratory service is the major factor which directly affects the quality of health care. The clinical laboratory as a whole has to provide the best patient care promoting excellence. METH...

journal_title：Clinical biochemistry

authors： Guzel O,Guner EI

更新日期：2009-03-01 00:00:00

abstract：:A procedure for the assay of glucose-6-phosphate dehydrogenase (EC 1.1.1.49) in erythrocytes on the GEMSAEC centrifugal analyzer using a modification of Beutler's procedure is described. Glucose-6-phosphate dehydrogenase activity is corrected for the contributing activity of 6-phosphogluconate dehydrogenase (EC 1.1.1....

journal_title：Clinical biochemistry

authors： Allen LC

更新日期：1979-10-01 00:00:00

abstract：OBJECTIVES:Hepcidin is the main regulator of systemic iron homeostasis and its expression is modulated by iron status, hypoxia, erythroid factors and inflammation. The aim of our study was to examine a relationship between level of hepcidin and iron status, erythropoietic activity, hypoxia and inflammation in exacerbat...

journal_title：Clinical biochemistry

authors： Tandara L,Grubisic TZ,Ivan G,Jurisic Z,Tandara M,Gugo K,Mladinov S,Salamunic I

更新日期：2015-12-01 00:00:00

abstract：OBJECTIVES:To monitor increases or decreases in cardiovascular disease (CVD)-related proteins that will be released from the deposits or plaque on the inner wall of blood vessels. DESIGN AND METHODS:Protein profiles of sera from healthy subjects and CVD patients were determined via 2-DE. Differentially expressed spots...

journal_title：Clinical biochemistry

authors： Kim HJ,Yoo HS,Kim PK,Kim MR,Lee HW,Kim CW

更新日期：2011-02-01 00:00:00

abstract：OBJECTIVE:To review biochemical literature and determine the significance of non-covalent bonding in plasma. RESULTS:Many non-covalent bonds exist in plasma. Relatively strong self-associations are found for serum albumin, several gamma-globulins, apolipoproteins and fibrin(ogen). Here, fibrinogen and fibrin, consider...

journal_title：Clinical biochemistry

authors： London M

更新日期：1997-03-01 00:00:00

abstract：:A simple and rapid method for serum sex hormone binding globulin (SHBG) was developed. Sera from pregnant women were pooled and the SHBG concentration was measured by Rosner's method. This pool was diluted with charcoal-treated and heated serum to give standards ranging in concentrations from 26.8-0.8 micrograms/L. Tw...

journal_title：Clinical biochemistry

authors： Zachariah NY,Thornblom RV,Chakmakjian ZH,Sumner GG

更新日期：1985-10-01 00:00:00

abstract：OBJECTIVE:Effects of metamizol and magnesium sulfate on erythrocyte glucose 6-phosphate dehydrogenase enzyme activity were investigated in in vitro and in vivo conditions. METHODS:For in vitro studies, glucose 6-phosphate dehydrogenase was purified from human erythrocyte and rats were used for in vivo studies. Enzyme ...

journal_title：Clinical biochemistry

authors： Ciftçi M,Ozmen I,Büyükokuroğlu ME,Pençe S,Küfrevioğlu OI

更新日期：2001-06-01 00:00:00

abstract：OBJECTIVES:To develop a tandem mass spectrometry (MS/MS) method for plasma free and total carnitine, incorporating acid hydrolysis for total carnitine measurement, for improved accuracy and sample throughput. DESIGN AND METHODS:Limit of detection (LOD), limit of quantitation (LOQ), linearity, accuracy, precision coeff...

journal_title：Clinical biochemistry

authors： Johnson DW

更新日期：2010-11-01 00:00:00

abstract：OBJECTIVES:To determine pediatric reference values for serum zinc concentration in Iranian subjects. DESIGN AND METHODS:Serum zinc concentration was measured by flame atomic absorption spectrometry in 699 children and adolescents. Reference values for serum zinc were determined according to the Clinical and Laboratory...

journal_title：Clinical biochemistry

authors： Ghasemi A,Zahediasl S,Hosseini-Esfahani F,Syedmoradi L,Azizi F

更新日期：2012-10-01 00:00:00

abstract：OBJECTIVES:To examine whether the proportions of blood leukocyte subsets are altered in COPD, and to assess the disturbances in signalling pathways in the leukocytes of COPD patients, with respect to smoking history. DESIGN AND METHODS:The study was performed at the Faculty of Pharmacy and Biochemistry and University ...

journal_title：Clinical biochemistry

authors： Rumora L,Milevoj L,Popović-Grle S,Barisić K,Cepelak I,Grubisić TZ

更新日期：2008-04-01 00:00:00

abstract：:1. We compare the Roche ion-exchange column chromatography and the Merck antibody inhibition reaction for CK-MB fractionation in 51 sera. Measurements of total CK and CK-MB activities must be done under the same conditions for each method in order to correlate the results. 2. A decisional value must be used for the in...

journal_title：Clinical biochemistry

authors： Desjarlais F,Daigneault R

更新日期：1979-08-01 00:00:00

abstract：OBJECTIVES:The aim of this study was to investigate the diagnostic utility of plasma neutrophil gelatinase-associated lipocalin (NGAL) as an early objective biomarker to predict acute kidney injury (AKI) in critically ill patients with suspected sepsis, for whom procalcitonin (PCT) was used for the diagnosis and stagin...

journal_title：Clinical biochemistry

authors： Kim H,Hur M,Cruz DN,Moon HW,Yun YM

更新日期：2013-10-01 00:00:00

abstract：OBJECTIVES:To determine salivary cortisol reference intervals in a healthy adult population, at 6 different time points during a 24-hour (h) period. METHODS:In a prospective study, salivary cortisol concentrations were measured upon waking, one-hour post-waking and at specific times of the day: at 12 h00, 16 h00, 20 h...

journal_title：Clinical biochemistry

authors： Gagnon N,Fréchette I,Mallet PL,Dubé J,Houde G,Fink GD

更新日期：2018-04-01 00:00:00

abstract：:A high pressure liquid chromatographic method for the determination of hydrochlorothiazide in serum has been elaborated and verified in a blood level study in normal individuals given a single oral dose of 50 mg hydrochlorothiazide. Based on 5 ml of serum, the limit of detection of the method is 2 ng/ml. ...

journal_title：Clinical biochemistry

authors： Robinson WT,Cosyns L

更新日期：1978-08-01 00:00:00