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Development of an Au/ZnO thin film surface plasmon resonance-based biosensor immunoassay for the detection of carbohydrate antigen 15-3 in human saliva.

Abstract：

OBJECTIVE:To evaluate a novel surface plasmon resonance (SPR) system for the direct measurement of tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva. DESIGN AND METHODS:We measured the presence of the tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva using 2 different surface plasmon resonance (SPR) systems. To compare the sensitivity of an SPR biosensor based on thin-film Au/ZnO and the Biacore SPR system, we prepared CA15-3 samples in saliva and analyzed intensity responses to the samples at various concentrations of CA15-3. RESULTS:The linear detection range of CA15-3 in human saliva with the SPR system based on thin-film Au/ZnO was 2.5-20 U/mL (the cut-off point in cancer patients is around 4 U/mL). The linear range with the Biacore SPR system was 40-300 U/mL. CONCLUSIONS:These results show that thin-film Au/ZnO-based SPR systems have higher sensitivity and can be used for measuring the levels of CA15-3 in human saliva without concentrating the samples.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Liang YH,Chang CC,Chen CC,Chu-Su Y,Lin CW

doi

10.1016/j.clinbiochem.2012.09.001

subject

Has Abstract

pub_date

2012-12-01 00:00:00

pages

1689-93

issue

18

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(12)00509-7

journal_volume

45

pub_type

杂志文章

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