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Solid phase measurements of antibody and lectin binding to xenogenic carbohydrate antigens.

Abstract：

OBJECTIVES:In future pig-to-man xenotransplantation it is important to master tools that identify potentially xenogenic alphagalactose (Galalpha) antigens in the doner tissue. DESIGN AND METHODS:We have measured the binding potentials of Galalpha detecting lectins and antibodies, including a naturally occurring subfraction from human serum, to Galalpha containing neoglycoproteins and mouse laminin that were immobilized on microtiter plates. RESULTS:Galalpha reactive antibodies with similar monosaccharide specificity have distinct structural preference for sugar ligands. Laminin and neoglycoproteins were treated with alpha-galactosidase and subsequently incubated with antibodies and lectins. The enzyme treatment was more deleterious on antibody binding than on lectin binding. CONCLUSION:Antibodies and lectins may bind to different galactose determinants on the glycoproteins. Two anti-Galalpha1 antibodies that both have been raised against glycans on rabbit red blood cells may recognize Galalpha-antigens with varying specificities. Binding results obtained after digestion with alpha-galactosidase indicate that some xenoreactive Galalpha groups are not directly accessible for removal by the enzyme.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Kirkeby S,André S,Gabius HJ

doi

10.1016/s0009-9120(03)00120-6

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

36-41

issue

1

eissn

0009-9120

issn

1873-2933

pii

S0009912003001206

journal_volume

37

pub_type

杂志文章

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