Abstract:
:MicroRNAs are endogenous non-coding RNAs that post-transcriptionally regulate gene expression by specifically binding the target mRNA and by consequently inducing its degradation. miRNAs can be released into the circulation where they remain stable and they can be measured. Their changes reflect individual biologic adaptation to exposures to specific environmental conditions. As such, measurement of circulating microRNAs represents an opportunity to evaluate biologic changes associated with interventions such as exercise and diet. Physical activity is, indeed, a very important modifying factor for circulating miRNAs. Toward their use in clinical settings several issues should be still solved. Their clinical application is hindered by the high heterogeneity of the analytical procedures used for their measurements. Furthermore, several pre-analytical concerns equally reduce the clinical applicability of miRNA. Pre-analytical phase in sports medicine is an important issue both because, often the conditions in which sampling are performed are peculiar (and not always canonical) and because some of the tested parameters, in the case of professional athletes, enters in routine anti-doping testing and, as such, they should be treated according to precise rules in order to avoid any false positive results. Aim of this review is to give an overview of the main available knowledges about the pre-analytical management of the sample for circulating miRNA evaluation along with the importance of miRNA as regulators of the response to physical activity and their possible future use in anti-doping settings.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Lombardi G,Perego S,Sansoni V,Banfi Gdoi
10.1016/j.clinbiochem.2016.09.017subject
Has Abstractpub_date
2016-12-01 00:00:00pages
1331-1339issue
18eissn
0009-9120issn
1873-2933pii
S0009-9120(16)30335-6journal_volume
49pub_type
杂志文章,评审abstract:OBJECTIVES:End-stage renal disease (ESRD) is characterized by profound dyslipidemia and enhanced oxidative stress. The patients also show evidence of exhausted and/or deficient anti-oxidative defense enzymes, one of them being glutathione-S-transferase (GST). This study investigates relationship between GST gene polymo...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2013.11.011
更新日期:2014-04-01 00:00:00
abstract:OBJECTIVE:Prostate-specific antigen (PSA) samples from five commercial sources were compared with PSA from the European Commission Community Bureau of Reference, known as CRM 613, using liquid chromatography-mass spectrometry (LC-MS) and matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS). DESIGN ...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2004.10.016
更新日期:2005-02-01 00:00:00
abstract:OBJECTIVES:The aim of the study was to examine the long-term effect of commonly used whole soy foods (soy flour) and purified daidzein (one major isoflavone and the precursor of equol) on renal function among prehypertensive postmenopausal women who are also equol producers, a population most likely to benefit from soy...
journal_title:Clinical biochemistry
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/j.clinbiochem.2014.05.054
更新日期:2014-09-01 00:00:00
abstract:OBJECTIVES:We investigated whether plasma visfatin and binding protein-4 (RBP-4) levels correlate with obesity and type 2 diabetes mellitus (T2DM). DESIGN AND METHODS:Two groups were enrolled: Group 1: 40 patients with T2DM and Group 2: 40 age- and gender-matched healthy controls. Both groups were subdivided according...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2011.08.1148
更新日期:2011-12-01 00:00:00
abstract::Medical errors are a prominent issue in health care. Numerous studies point at the high prevalence of adverse events, many of which are preventable. Although there is a range of severity in errors, they all cause harm, to the patient, to the system, or both. While errors have many causes, including human interactions ...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2013.03.025
更新日期:2013-09-01 00:00:00
abstract::Kallikreins are serine proteases with diverse physiologic functions. They are represented by multigene families in many animal species, especially in rat and mouse. Recently, the human kallikrein gene family has been fully characterized and includes 15 members, tandemly localized on chromosome 19q13.4. A new definitio...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/s0009-9120(03)00055-9
更新日期:2003-09-01 00:00:00
abstract:OBJECTIVES:Does abnormal betaine excretion persist? DESIGN AND METHODS:Patients (10) with abnormal excretion in 1998 were recalled in 2005. Subsequent urine samples were collected on 4 days from persistently abnormal subjects. RESULTS:Half the 1998 abnormal patients were abnormal in 2005. Only 1/20 controls was abnor...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2007.03.009
更新日期:2007-07-01 00:00:00
abstract:BACKGROUND:The clinical laboratory is responsible for reporting accurate and expeditious results. However, the pre-analytical phase is directly related to the procedure of specimen collection and is mostly out of the direct control of the laboratory; further, most pre-analytical errors are related to human factors. The...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2019.05.016
更新日期:2019-08-01 00:00:00
abstract:OBJECTIVES:Renal dysfunction has been reported in normoalbuminuric patients, demonstrating the necessity to improve the diagnostic and prognostic tools for diabetic kidney disease (DKD) investigation. Therefore, the aim of this study was to investigate whether the urinary levels of neutrophil gelatinase-associated lipo...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2015.10.016
更新日期:2016-02-01 00:00:00
abstract::57Co-cyanocobalamin is substituted for iodine labeled antigen in three commercially available solid phase radioimmunoassay kits to evaluate the extent of non-specific binding by determination of the supernatant and residual fraction of unbound ligand and thus obtain a value for corrected binding throughout the range o...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(78)90070-x
更新日期:1978-06-01 00:00:00
abstract:OBJECTIVE:To confirm that the naproxen metabolite O-desmethylnaproxen interferes with the Jendrassik and Grof method for total bilirubin and was a possible cause of spurious hyperbilirubinemia in a case of naproxen overdose. DESIGN AND METHODS:Plasma was spiked with naproxen or O-desmethylnaproxen before total and dir...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2008.09.119
更新日期:2009-01-01 00:00:00
abstract:OBJECTIVES:To determine pediatric reference values for serum zinc concentration in Iranian subjects. DESIGN AND METHODS:Serum zinc concentration was measured by flame atomic absorption spectrometry in 699 children and adolescents. Reference values for serum zinc were determined according to the Clinical and Laboratory...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2012.07.086
更新日期:2012-10-01 00:00:00
abstract:OBJECTIVES:Hypertension (HT) is a complex disorder influenced by both genetic and environmental factors. Recent genome-wide association studies have identified a major risk locus for atherosclerosis on chromosome 9p21.3. SNPs within the coding sequences of CDKN2A/B and the long non-coding RNA CDKN2B-AS1 could potential...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2016.02.012
更新日期:2016-07-01 00:00:00
abstract:OBJECTIVES:Low-normal thyroid function may relate to increases in plasma cholesterol and triglycerides, but effects on lipoprotein subfractions are largely unknown. Associations of alterations in lipoprotein metabolism and functionality with low-normal thyroid function could be more pronounced in Type 2 diabetes mellit...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2015.01.015
更新日期:2015-05-01 00:00:00
abstract::Food allergy has a dramatic impact on a child's (and their family's) quality of life and places a major financial burden on the community. It has been hypothesized that the increase in food allergy may relate to the concordant rise in prevalence of vitamin D insufficiency. More recently a second hypothesis has implica...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2014.01.033
更新日期:2014-06-01 00:00:00
abstract:OBJECTIVES:The plasma apolipoprotein B (apo B) concentrations have been considered to be a more accurate representation of atherogenic particles and it has been proposed that the formula LDL-C (mmol/L) = 0.41TC - 0.32TG + 1.70apo B - 0.27 is reliable for the estimation of LDL-C (Clin Chem 1997; 43: 808-15). We undertoo...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(00)00162-4
更新日期:2000-10-01 00:00:00
abstract::Congestive heart failure usually features a mild to moderate increase in aspartate aminotransferase (AST) activity. A marked increase in AST may occur rarely and is usually preceded by prolonged shock or hypotensive episodes and is accompanied by hyperbilirubinemia. I report two patients with congestive heart failure ...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(83)90366-1
更新日期:1983-04-01 00:00:00
abstract::We have modified the procedure for determining total metanephrines in urine described by Gupta, Price and Keane(1) to shorten the time necessary for completion while allowing the technician time to perform other tests. The modification involves the extraction of interfering substances from urine. Instead of having to ...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(76)80029-x
更新日期:1976-04-01 00:00:00
abstract:OBJECTIVES:Reporting a case of inherited factor V deficiency and galactosemia. METHODS:A neonate was admitted with hematoma, jaundice, splenomegaly, diarrhea, anemia, abdominal ascites and bilateral cataracts that diagnosis of galactosaemia and factor V deficiency was established. RESULTS:Coinheritance of both coagul...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2011.12.024
更新日期:2012-03-01 00:00:00
abstract:OBJECTIVE:Plasma ammonia measurement is greatly influenced by pre-analytical conditions, which may lead to false positives. We wanted to evaluate the prevalence and clinical impact of plasma ammonia false positives in a pediatric population. DESIGN AND METHODS:Over a 28-month period, charts of patients with elevated a...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2007.01.024
更新日期:2007-05-01 00:00:00
abstract:OBJECTIVES:The relationship between glycated hemoglobin A1c (HbA1c) and average glucose has been described by the empirically derived estimated average glucose (eAG) equation in the A1c-Derived Average Glucose (ADAG) study, with extensive calibration efforts in four secondary reference HbA1c methods. It is not known if...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2015.02.012
更新日期:2015-05-01 00:00:00
abstract:OBJECTIVE:To increase the accuracy of non-invasive diagnosis of nonalcoholic fatty liver disease (NAFLD), clinical and laboratory NAFLD indicators were integrated into a diagnostic formula. METHODS:A total of 141 patients with clinically diagnosed NAFLD and 30 healthy controls were enrolled. We collected case history,...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2018.11.011
更新日期:2019-02-01 00:00:00
abstract:BACKGROUND:Exercise is a prone condition to enhanced oxidative stress and damage and the specific activity pattern of a soccer match may favour additional pro-oxidant redox alterations. To date, no studies have reported the impact of a soccer match on oxidative stress and muscle damage markers. AIM:To analyse the effe...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2008.04.008
更新日期:2008-07-01 00:00:00
abstract::Fucosidosis is an inherited lysosomal storage disease due to a deficiency of alpha-L-fucosidase activity. Exponentially growing lymphoid cell cultures from a fucosidosis patient (JH) had 16-fold lower extracellular alpha-L-fucosidase protein and 72-fold lower intracellular alpha-L-fucosidase protein with negligible ca...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/0009-9120(91)80018-x
更新日期:1991-06-01 00:00:00
abstract:OBJECTIVES:Define performance characteristics of the Stratus CS cardiac troponin I (cTnI) assay in routine practice. METHODS:Imprecision at low cTnI values was assessed according to NCCLS EP5-A. Linearity was assessed by regression analysis; the reference interval was determined in 345 healthy individuals. Minimum det...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2004.03.009
更新日期:2004-08-01 00:00:00
abstract::This review focusses on research performed by the author and coworkers. The absorption, turnover and excretion of cobalamin and the pathogenesis of cobalamin deficiency states are described and the laboratory tests used to diagnose these states are discussed. Topics dealt with in detail include: overall turnover, dail...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/s0009-9120(84)90279-0
更新日期:1984-04-01 00:00:00
abstract:BACKGROUND:Numerous etiologies are implicated in the complications of diabetes. A link between diabetes and the predisposition to certain cancers, including colon cancer, has been established during the last decade. However, the relationship between fecal occult blood test (FOBT), a screening method used principally fo...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2016.10.005
更新日期:2017-01-01 00:00:00
abstract:OBJECTIVES:Abnormal alanine aminotransferase (ALT) activity is indicative of liver disease even a burden of overall health. We assessed the factors associated with ALT activity and their internal relationships in a male population from southern China. DESIGN AND METHODS:Data of physical examinations, laboratory tests,...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2012.08.022
更新日期:2012-12-01 00:00:00
abstract:BACKGROUND:Vitamin D (25-OHD) physiological functions have been expanded beyond traditional bone health, increasing the importance of its estimation in Laboratory Medicine, which renders validation of available methods mandatory. AIMS AND METHODS:We evaluated some preanalytical and analytical aspects of 25-OHD determi...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2012.08.003
更新日期:2012-12-01 00:00:00
abstract:OBJECTIVES:The purpose of this study was to measure the extent of lipid peroxidation and the status of antioxidants in patients with Hodgkin's disease. DESIGN AND METHODS:Glutathione peroxidase (GPX) and superoxide dismutase (SOD) activities, and malondialdehyde (MDA), selenium, zinc and copper content have been measu...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(00)00062-x
更新日期:2000-04-01 00:00:00