Abstract:
OBJECTIVES:The relationship between glycated hemoglobin A1c (HbA1c) and average glucose has been described by the empirically derived estimated average glucose (eAG) equation in the A1c-Derived Average Glucose (ADAG) study, with extensive calibration efforts in four secondary reference HbA1c methods. It is not known if this relationship is preserved when HbA1c is measured by routine laboratory methods under routine conditions. DESIGN AND METHODS:We measured average glucose (mAG) by six days of continuous glucose monitoring in 45 adults with stable HbA1c (<1% HbA1c change in the preceding two months). Subjects with medical conditions that may confound HbA1c measurement, including anemia and hemoglobinopathy, were excluded. HbA1c was measured using Bio-Rad Variant II (cation-exchange HPLC), Bio-Rad in2it (boronate affinity HPLC) and Roche Tina-quant (immunoassay) methods. RESULTS:The average differences between eAG derived from the routine HbA1c methods and mAG were 10.4% (Variant II), 6.0% (Tina-quant) and 1.0% (in2it). The regression coefficients between the mAG and HbA1c are different between in2it (mAG, mmol/L=0.58 × %HbA1c + 2.3), Tina-quant and Variant II (both mAG, mmol/L=0.66 × %HbA1c + 1.9). However, the 95% confidence intervals of the slope and bias of these methods overlap. The correlation between mAG and HbA1c was greatest when measured using the Variant II (r(2)=0.84), followed by Tina-quant (r(2)=0.82) and in2it (r(2)=0.71). CONCLUSIONS:The relationship between HbA1c and measured average glucose is method-dependent despite improved HbA1c standardization. The differences in relationship may reflect as discrepant eAG and home glucose monitoring results.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Loh TP,Sethi SK,Wong MS,Tai ES,Kao SLdoi
10.1016/j.clinbiochem.2015.02.012subject
Has Abstractpub_date
2015-05-01 00:00:00pages
514-8issue
7-8eissn
0009-9120issn
1873-2933pii
S0009-9120(15)00066-1journal_volume
48pub_type
杂志文章abstract:AIMS:There is increasing interest in using fructosamine measurements in screening for or managing diabetes, yet uncertainty remains as to whether these measurements should be corrected for variation in serum protein concentrations. METHODS:We considered all sets of simultaneous measurements of fructosamine, albumin, t...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2016.10.014
更新日期:2017-02-01 00:00:00
abstract:OBJECTIVES:To develop and validate a novel genotyping approach, named infrared Fluorescence Allele Specific Hybridization (iFLASH), which combines the principles of allele specific oligonucleotide (ASO) hybridization with the advanced possibilities of infrared imaging. DESIGN AND METHODS:As an example, we genotyped th...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2006.02.012
更新日期:2006-07-01 00:00:00
abstract:OBJECTIVES:The non-invasive differentiation of ischemic and non-ischemic acute heart failure (AHF) not resulting from acute myocardial infarction is difficult and has therapeutic and prognostic implications. The aim of this study was to assess whether plasma B-type natriuretic peptide (BNP) can identify ischemic etiolo...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2014.01.025
更新日期:2014-04-01 00:00:00
abstract:OBJECTIVES:Hepatic overproduction of apolipoprotein B (apoB)-containing lipoproteins appears to be a common cause of hyperlipoproteinemia in humans. Patients with overproduction states secrete denser cholesterol ester-rich lipoprotein particles which are highly atherogenic. The formation of apoB particles involves a ve...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/0009-9120(95)00007-v
更新日期:1995-04-01 00:00:00
abstract:OBJECTIVES:To investigate the association of parental hyperhomocysteinemia, C677T Methylene tetrahydrofolate reductase (MTHFR) polymorphism and DNA damage with recurrent pregnancy loss (RPL). DESIGN AND METHODS:A case-control study. Reverse phase HPLC, PCR-RFLP and Cytokinesis blocked micronuclei assay were used to as...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2008.12.003
更新日期:2009-03-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to determine the adult reference values for cystatin C (CysC) and to evaluate their consistence with those reported in the literature. DESIGN AND METHODS:CysC was analyzed in a consecutive series of subjects (100 males and 100 females) by a nephelometric immunoassay. Medline was sea...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2007.05.011
更新日期:2007-09-01 00:00:00
abstract:OBJECTIVES:Molecular screening plays a major role in prognostic categorization and subsequent definition of treatment strategies for acute myeloid leukemia. The possibility of using IDH1/2 mutations as a marker for the monitoring of minimal residual disease (MRD) is still under investigation and remains unclear. METHO...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2018.08.012
更新日期:2018-11-01 00:00:00
abstract:OBJECTIVES:Newly presented solid-phase extraction methods (Solid Phase Disc Extraction, SPEC-Plus, Multi-Modal, and Solid Phase Micro Extraction, SPME have been checked with respect to their applicability to clinical toxicological analysis. In comparison with conventional liquid/liquid-extraction and a common mixed-pha...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(96)00096-3
更新日期:1996-12-01 00:00:00
abstract::The discovery of the causal association of adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP) deficiency with some forms of primary immunodeficiency disease had led to new approaches to therapy, such as enzyme replacement. In ADA deficiency, bone marrow transplantation remains the primary method of ch...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/s0009-9120(83)94381-3
更新日期:1983-02-01 00:00:00
abstract:BACKGROUND:Polycyclic aromatic hydrocarbons (PAHs), some ubiquitous environmental contaminants are capable to cause oxidative stress, during its metabolism. It is believed that many diseases that have a common origin in oxidative stress begin in childhood. Considering oxidative stress evolved during PAHs metabolism as ...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2007.11.017
更新日期:2008-02-01 00:00:00
abstract:OBJECTIVES:The circulating lipoproteins may cause some abnormalities in platelet composition and function in hypercholesterolemia. The aim of this study was to investigate whether platelet apoptosis, platelet activation, platelet aggregation, platelet-leukocyte aggregate (PLA) formation and lipid peroxidation occur sim...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2005.09.005
更新日期:2005-12-01 00:00:00
abstract:OBJECTIVES:Elevated advanced glycation endproducts (AGEs) are implicated in diabetic complications. Methylglyoxal-derived hydroimidazolone (MG-H) is one of the most abundant AGEs in vivo. Our objective was to develop a time-saving, specific method to measure free MG-H in plasma and determine its levels in complication-...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2008.12.016
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND:Gliomas are tumors of the central nervous system derived from glial cells. They show cellular heterogeneity and lack specific diagnostic markers. Although a possible role for the eicosanoid cascade has been suggested in glioma tumorigenesis, the relationship between enzymes and receptors implicated in arachi...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2010.03.017
更新日期:2010-07-01 00:00:00
abstract:OBJECTIVES:Renal dysfunction has been reported in normoalbuminuric patients, demonstrating the necessity to improve the diagnostic and prognostic tools for diabetic kidney disease (DKD) investigation. Therefore, the aim of this study was to investigate whether the urinary levels of neutrophil gelatinase-associated lipo...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2015.10.016
更新日期:2016-02-01 00:00:00
abstract:BACKGROUND:Elevations of serum cardiac troponin T (cTnT) have been described in patients with end stage chronic kidney disease (CKD) although the mechanism is unknown, whether from increased production or decreased clearance. Less is known about cTnT in short term changes in renal function and in lesser degrees of rena...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2015.05.004
更新日期:2015-08-01 00:00:00
abstract:OBJECTIVES:Reporting a case of inherited factor V deficiency and galactosemia. METHODS:A neonate was admitted with hematoma, jaundice, splenomegaly, diarrhea, anemia, abdominal ascites and bilateral cataracts that diagnosis of galactosaemia and factor V deficiency was established. RESULTS:Coinheritance of both coagul...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2011.12.024
更新日期:2012-03-01 00:00:00
abstract:OBJECTIVE:MicroRNA-331 (miR-331) has shown regulatory activity against several genes whose expression has been claimed to be deregulated in breast tumors, including that of epidermal growth factor receptor 2 (HER2). Herein, the clinical value of miR-331 expression was investigated by analyzing its levels in breast beni...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2018.07.008
更新日期:2018-09-01 00:00:00
abstract::Increased hemoglobin A1 (HbA1) occurs in diabetes mellitus as a direct consequence of hyperglycemia, which causes postsynthetic modification of hemoglobin proportional to blood glucose concentration. Glycosylation of hemoglobin is demonstrable also in vitro, not only by glucose, but with other reducing sugars such as ...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(82)90556-2
更新日期:1982-06-01 00:00:00
abstract:OBJECTIVES:Accurate measurement of IgG subclass (IgGSc) levels are essential to aid in the diagnosis of disease states such as primary immunodeficiencies. However, there is no single standardisation of nephelometric and turbidimetric assays for these analytes and two reference materials have been utilised. We expand on...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2013.07.014
更新日期:2013-11-01 00:00:00
abstract::The influence of age and disease on serum albumin and alpha-1-acid glycoprotein was studied in 126 subjects (69 M, 57 F) presenting with tuberculosis and after recovery. Albumin concentration fell with age in both disease and healthy states, and alpha-1-acid glycoprotein concentration rose with age in the healthy stat...
journal_title:Clinical biochemistry
pub_type: 临床试验,杂志文章
doi:10.1016/0009-9120(94)90032-9
更新日期:1994-08-01 00:00:00
abstract:BACKGROUND:The immunosuppressive activity of mycophenolate mofetil (MMF) is based on the reversible inhibition of inosine 5'-monophosphate dehydrogenase (IMPDH) by mycophenolic acid (MPA). It was the aim of this study to develop a nonradioactive method for specific measurement of IMPDH activity in isolated peripheral m...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(01)00267-3
更新日期:2001-10-01 00:00:00
abstract:BACKGROUND:Patients suffering from hemorrhagic fever with renal syndrome (HFRS) often showed strikingly reduced high-density lipoprotein (HDL)-cholesterol levels during the oliguric phase, indicating severe alterations in lipoprotein metabolism. OBJECTIVE:To compare changes in the functions and composition of HDL, lip...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2007.10.007
更新日期:2008-01-01 00:00:00
abstract::Collagen, the most abundant protein of the mammalian body, is specifically degraded by collagenase. Collagenase activity and subsequent collagen degradation are the main aspects of essential biological processes such as bone remodeling, tissue repair and wound healing. Measurement of collagenase activity is of interes...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(05)80002-5
更新日期:1991-12-01 00:00:00
abstract::Autosomal dominant olivopontocerebellar degeneration was evaluated in a family numbering 414 living members. Urine samples were obtained from 5 affected and 5 unaffected not at-risk family members after 4 days on a synthetic diet. 159 urine vapor constituents were measured by gas-liquid and ion-exchange chromatography...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(75)93752-2
更新日期:1975-12-01 00:00:00
abstract:OBJECTIVE:Apolipoprotein E (APOE) E4, apolipoprotein B-100 (APOB) Q3611 allele, the angiotensin converting enzyme (ACE) deletion (D) allele and glycoprotein IIIa (GP3A) P33 mutant allele are reported to predispose to early-onset coronary heart disease (CHD). These associations were not all confirmed in more recent stud...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(99)00017-x
更新日期:1999-06-01 00:00:00
abstract:OBJECTIVES:To compare a fully automated immunoassay and a fully automated HPLC method for the quantification of HbA1c. DESIGN AND METHODS:For an inter-method comparison, 200 consecutive clinical samples were analyzed. Imprecision was studied with pooled whole blood. RESULTS:Very close agreement of results (identical ...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2008.08.084
更新日期:2008-11-01 00:00:00
abstract:OBJECTIVE:To investigate the feasibility of detecting circulating cancer cells in peripheral blood from gastric cancer patients using Piwi-interacting RNAs (piRNAs) as markers. DESIGN AND METHODS:Real-time quantitative reverse transcription-polymerase chain reaction was used to analyze piR-651 and piR-823 levels in th...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2011.06.004
更新日期:2011-09-01 00:00:00
abstract:OBJECTIVE:This article will review the advancements and new developments being made in (1) advanced computers, (2) microtechnology, (3) advanced immunodiagnostics, (4) neural networks, and (5) molecular biology. The influence of these technologies and their products on clinical laboratories is also discussed. CONCLUSI...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/0009-9120(94)00075-7
更新日期:1995-06-01 00:00:00
abstract:OBJECTIVES:The aim of our study was to assess the association of common sequence variants, and selected interactions, with HDL-c plasma levels. DESIGN AND METHODS:We analysed 743 individuals (340 men and 403 women) with high mean triglyceride and LDL-c levels. The association of five polymorphic sites (ABCA1 g.1051G>A...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2010.04.001
更新日期:2010-06-01 00:00:00
abstract:OBJECTIVES:To evaluate the evidence concerning gFOBT kits and to develop gFOBT standards to be implemented by the Ontario Colorectal Cancer (CRC) Screening Program (the "Program"). DESIGN AND METHODS:We evaluated 3 aspects of gFOBT kits: 1) performance factors; 2) usability factors; and 3) laboratory factors. We obtai...
journal_title:Clinical biochemistry
pub_type: 杂志文章,meta分析
doi:10.1016/j.clinbiochem.2008.08.069
更新日期:2008-11-01 00:00:00