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Purification and characterization of cyclosporine and FK-506 binding proteins from a human T-helper cell line.

Abstract：

:Cytosolic proteins that specifically bind cyclosporine A and FK-506 were isolated and purified from the JURKAT human T-helper cell line. These binding proteins were purified by affinity, molecular weight exclusion and weak cation exchange column chromatography. Radiolabeled cyclosporine A specifically bound to a approximately 17 kDa molecule which is cyclophilin and also bound to a approximately 50 kDa protein(s). Radiolabeled FK-506 did not bind to the approximately 17 kDa molecular weight protein, but specifically bound to soluble approximately 10 kDa and approximately 50 kDa proteins.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Palaszynski EW,Donnelly JG,Soldin SJ

doi

10.1016/0009-9120(91)90252-a

subject

Has Abstract

pub_date

1991-02-01 00:00:00

pages

63-70

issue

1

eissn

0009-9120

issn

1873-2933

pii

0009-9120(91)90252-A

journal_volume

24

pub_type

杂志文章

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