Abstract：
OBJECTIVES:Bilirubin exerts anti-oxidative and anti-inflammatory properties which may beneficially influence the development of cardio-metabolic disorders. A nuclear magnetic resonance (NMR) spectroscopy-based glycoprotein biomarker, designated GlycA, whose signal originates from several glycosylated acute-phase proteins, has been recently developed. We tested whether plasma GlycA is associated with bilirubin in subjects with and without MetS. DESIGN AND METHODS:GlycA (NMR spectroscopy), high sensitivity C-reactive protein (hs-CRP) and bilirubin were measured in 58 fasting subjects with MetS and in 63 subjects without MetS (including 65 subjects with type 2 diabetes mellitus). RESULTS:GlycA and hs-CRP were higher, coinciding with lower bilirubin in MetS (p<0.01 for each). In all subjects combined, GlycA was strongly correlated with hs-CRP (r=0.631, p<0.001). Age-, sex- and diabetes status-adjusted multivariable linear regression analysis demonstrated that GlycA and hs-CRP were both associated positively with the presence of MetS (β=0.256, p=0.014 and β=0.259, p=0.012, respectively). GlycA and hs-CRP were negatively related to bilirubin (β=-0.258, p=0.007 and β=-0.305, p<0.001, respectively), independent of MetS (p>0.05 for each) and diabetes status (p>0.50 for each). CONCLUSIONS:GlycA is elevated in MetS, and may represent a quantitative measure of a pro-inflammatory state. Increased levels of glycosylated acute-phase proteins are associated with lower bilirubin in MetS.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Dullaart RP,Gruppen EG,Connelly MA,Lefrandt JDdoi
10.1016/j.clinbiochem.2015.06.016subject
Has Abstractpub_date
2015-11-01 00:00:00pages
1045-7issue
16-17eissn
0009-9120issn
1873-2933pii
S0009-9120(15)00254-4journal_volume
48pub_type
杂志文章abstract：:New methods were developed for the separation of major lipid classes varying in polarity from cholesterol esters to lysophosphatidylcholine. The methods were used for the analysis of extracts obtained from human sera. The lipids were separated by overpressured thin-layer chromatography, classical thin-layer chromatogr...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(88)80092-4
更新日期：1988-04-01 00:00:00
abstract：OBJECTIVES:To report the clinical and laboratory characteristics of urea cycle disorder (UCD) patients at a tertiary care center in a developing country. DESIGN AND METHODS:Retrospective study of clinical and laboratory data of UCD patients. RESULTS:Thirty-seven UCD patients were studied, 31 symptomatic (high risk) p...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2009.12.004
更新日期：2010-03-01 00:00:00
abstract：OBJECTIVE:To develop sensitive nonisotopic assays for protein-tyrosine phosphatase (PTP) activity. METHODS:The fluorometric assay is based on the fact that phosphotyrosine but not tyrosine forms highly fluorescent complexes with Tb3+. Thus, PTP activity can be followed by measuring the decrease of fluorescence due to ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(95)02026-8
更新日期：1996-04-01 00:00:00
abstract：:We describe a simple and reliable radioimmunoassay (RIA) method for the measurement of human placental lactogen (hPL) in undiluted serum. Standard curves, in serum, cover the range of 0.015 to 1 mlU/L for early pregnancy monitoring (7 to 15 weeks, referred to as Procedure I), and 0.531 to 17 mIU/L for mid and late pre...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(82)90753-6
更新日期：1982-04-01 00:00:00
abstract：OBJECTIVES:The glutathione S-transferases (GSTs) play a critical role in protecting the colorectal mucosa. We investigated the efficacy of using the GSTs activity of plasma as a biomarker of risk for colorectal cancer. METHODS:GSTs activity was measured in the plasma of control individuals (n=39) and in the plasma, tu...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2005.04.004
更新日期：2005-07-01 00:00:00
abstract：OBJECTIVE:Mycophenolic acid requires routine therapeutic drug monitoring. We evaluated the suitability of a new PETINIA (particle enhanced turbidimetric inhibition immunoassay) assay on the Dimension EXL analyzer for monitoring of mycophenolic acid by comparing values obtained by this assay with a HPLC-UV method. DESI...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2012.11.025
更新日期：2013-05-01 00:00:00
abstract：OBJECTIVES:Concentrations of troponin measured with high sensitivity troponin assays are raised in a number of emergency department (ED) patients; however many are not diagnosed with acute myocardial infarction (AMI). Clinical comparisons between the early use (2h after presentation) of high sensitivity cardiac troponi...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2013.11.019
更新日期：2014-04-01 00:00:00
abstract：:A simple method for the determination of triodothyronine (T3) uptake using I125-T3 and dextran coated charcoal is described. This reaction is time dependent after the addition of dextran coated charcoal. Effect of temperature, concentrations of I125-T3 and charcoal are reported. Within-run precision has been estimated...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(76)80009-4
更新日期：1976-02-01 00:00:00
abstract：CONTEXT:Knee arthroplasty surgery is significant trauma, leading to an activated immune system causing inflammation and oxidative stress. Many current biomarkers are invasive, costly, and often slow to analyse, limiting their use for rapid inflammatory measurements. OBJECTIVES:We have examined the use of urinary neopt...
journal_title：Clinical biochemistry
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1016/j.clinbiochem.2018.11.002
更新日期：2019-01-01 00:00:00
abstract：OBJECTIVES:Reporting a case of inherited factor V deficiency and galactosemia. METHODS:A neonate was admitted with hematoma, jaundice, splenomegaly, diarrhea, anemia, abdominal ascites and bilateral cataracts that diagnosis of galactosaemia and factor V deficiency was established. RESULTS:Coinheritance of both coagul...
journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.clinbiochem.2011.12.024
更新日期：2012-03-01 00:00:00
abstract：:1. The following proteolytic enzymes were measured in muscles of control subjects and patients with muscular dystrophies and related neuromuscular diseases: an elastase-like enzyme, carboxypeptidase A, carboxypeptidase B and pyroglutamyl peptidase. 2. Elastase-like enzyme and carboxypeptidase B did not show significan...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(79)80001-6
更新日期：1979-04-01 00:00:00
abstract：OBJECTIVES:We undertook the present study to investigate the possible relation between total antioxidant capacity (TAC) and tumor marker (TM) values in serum samples of patients with and without gastrointestinal tract and prostate tumors. DESIGN AND METHODS:We measured the TAC using trolox equivalent antioxidant capac...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2011.06.082
更新日期：2011-09-01 00:00:00
abstract：:The initial step in the action of thyrotropin (TSH) is its binding to the TSH receptor. TSH receptor antibodies are detected in up to 90% of patients with Graves' disease. Serial measurements of TSH receptor antibodies in patients with Graves' hyperthyroidism are helpful in predicting relapse. The TSH receptor was pur...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(90)90417-s
更新日期：1990-02-01 00:00:00
abstract：:This review discusses the question: which steps are essential or need to be optimized so that maternal plasma RNA sequencing for pre-eclampsia and related syndromes will become as specific and sensitive as maternal plasma DNA sequencing for trisomy? ...
journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.clinbiochem.2015.03.004
更新日期：2015-10-01 00:00:00
abstract：OBJECTIVE:To evaluate the clinical use of Homocysteine-Primavette, a new blood collection medium for total homocysteine (tHcy) assay. METHODS:The agreement between baseline tHcy and tHcy in stabilized samples (40 h) was assessed for FPIA, HPLC, GC-MS, LC-MS, and ICL. RESULTS:tHcy concentrations in whole blood were st...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2007.03.005
更新日期：2007-06-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to determine whether the atherogenic index of plasma (AIP=log[triglycerides/HDL-cholesterol]) differs in heterozygous familial hypercholesterolemia (FH) patients with and without a history of cardiovascular disease (CVD). DESIGN AND METHODS:A total of 555 FH patients with known mut...
journal_title：Clinical biochemistry
pub_type： 杂志文章,多中心研究
doi：10.1016/j.clinbiochem.2011.11.001
更新日期：2012-01-01 00:00:00
abstract：OBJECTIVE:This study was conducted to investigate the potential role of intravascular oxidative stress in inexplicable episodes of coronary artery spasm (CAS) during coronary angiography (CAG). DESIGN AND METHODS:Serial patients with stable angina scheduled for routine CAG were prospectively evaluated. Patients with c...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2009.01.021
更新日期：2009-05-01 00:00:00
abstract：OBJECTIVE:To determine Retinol Binding Protein 4 (RBP4) levels in patients with Familial Partial Lipodystrophy (FPLD). METHODS:Ten patients with FPLD and a control group (9 patients) were selected to participate in the study. RESULTS:RBP4-log levels were lower in patients with FPLD in comparison to control group (1.5...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2009.03.006
更新日期：2009-07-01 00:00:00
abstract：OBJECTIVES:To investigate the polymorphism distribution of Val-9Ala and Ile+58Thr of the Mn-superoxide dismutase (Mn-SOD) gene among subjects with Parkinson's disease (PD) by analyses of genders and clinical severity. DESIGN AND METHODS:We examined the DNA genotypes of Val-9Ala and Ile+58Thr from 295 PD subjects and 1...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2010.05.009
更新日期：2010-08-01 00:00:00
abstract：OBJECTIVES:To develop a method for the determination of succinylacetone (SA) in dried blood spots (DBS) using tandem mass spectrometry (MS/MS). METHODS:SA was extracted from DBS with an acetonitrile and water solution (80:20 by volume) containing formic acid and hydrazine hydrate (both at 0.1% by volume), and analyzed...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2004.07.006
更新日期：2004-11-01 00:00:00
abstract：:The use of alcohol and tobacco is prevalent among pregnant women despite the well-known adverse effects of these substances imposed on the developing fetus and immense public health education efforts. Confirmation of gestational exposures to these compounds have relied mostly on maternal self-reporting, which is often...
journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.clinbiochem.2004.01.010
更新日期：2004-06-01 00:00:00
abstract：OBJECTIVES:To determine the impact of long distance rowing (160km, nonstop) on standard biological parameters and to study the relation between inflammation, myocardial necrosis, lipid profile, heart rate and energy expenditure. METHODS:Electrolytes, lipid profile, C-reactive protein (CRP), interleukin-6 (IL-6), tumor...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2017.11.018
更新日期：2018-02-01 00:00:00
abstract：OBJECTIVE:To identify independent predictors of serum butyrylcholinesterase (BuChE) activity and examine its relationship with the metabolic syndrome. DESIGN AND METHODS:Serum BuChE activity was measured and metabolic syndrome risk factors were assessed in 1097 healthy subjects recruited from the Newfoundland adult po...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2005.04.008
更新日期：2005-09-01 00:00:00
abstract：OBJECTIVES:Lipoprotein(a) [Lp(a)] can be oxidized by copper in vitro in a way comparable to low-density lipoprotein (LDL). We sought to determine whether the susceptibility of Lp(a) to oxidation is correlated with the susceptibility of autologous heterogeneous LDL, with apolipoprotein(a) [apo(a)] molecular size, or wit...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(02)00438-1
更新日期：2003-03-01 00:00:00
abstract：OBJECTIVES:Although monitoring of cyclosporin (CsA) is standard clinical practice postrenal transplantation, mycophenolic acid (MPA) concentrations are not routinely measured. There is evidence that a relationship exists between MPA area under the concentration-time curve (AUC) and rejection. In this study, a retrospec...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(00)00196-x
更新日期：2001-02-01 00:00:00
abstract：OBJECTIVES:Eales' disease (ED) is an idiopathic retinal vasculitis condition, which affects retina of young adult males. The histopathological hallmark in ED is the adhesion of leukocytes to the endothelium and the infiltration of these cells into the retinal parenchyma. Phagocyte generated free radicals have been impl...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(03)00058-4
更新日期：2003-07-01 00:00:00
abstract：OBJECTIVES:The newly developed glomerular filtration rate (GFR)-estimating equations developed by the CKD-EPI Collaboration and Feng et al. (2013) that are based on standardized serum cystatin C (ScysC), combined/not combined with serum creatinine (Scr), require further validation in China. We compared the performance ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2014.05.060
更新日期：2014-09-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to measure soluble receptor for advanced glycation end products (sRAGE) in plasma and synovial fluid of knee osteoarthritis (OA) patients and to determine the correlation between sRAGE levels and disease severity. DESIGN AND METHODS:Thirty-six OA patients and 15 healthy controls we...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2010.07.007
更新日期：2010-09-01 00:00:00
abstract：BACKGROUND:Niemann-Pick type C (NP-C) is a rare progressive neurodegenerative lipid storage disorder with heterogeneous clinical presentation and challenging diagnostic procedures. Recently oxysterols have been reported to be specific biomarkers for NP-C but knowledge on the intra-individual variation and on reference ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2015.03.007
更新日期：2015-06-01 00:00:00
abstract：OBJECTIVE:To evaluate if peak characteristics, the retention time, and percentage of total hemoglobin of an unknown peak on the Bio-Rad VARIANT II Hb A1c method may be used to establish a tentative identification of hemoglobin variants. METHOD:The peak characteristics, retention time, and percentage of total hemoglobi...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2004.11.004
更新日期：2005-03-01 00:00:00
