Abstract:
CONTEXT:Knee arthroplasty surgery is significant trauma, leading to an activated immune system causing inflammation and oxidative stress. Many current biomarkers are invasive, costly, and often slow to analyse, limiting their use for rapid inflammatory measurements. OBJECTIVES:We have examined the use of urinary neopterin and total neopterin in knee arthroplasty patients to non-invasively measure oxidative stress and inflammation from immune system activation. We aim to validate the use of these biomarkers for quick, cost effective and predictive measurements of post-surgical inflammation assessment. METHODOLOGY:19 Knee arthroplasty patients were analysed pre-operatively and for a defined post-operative period to determine the urinary levels of neopterin and total neopterin (neopterin +7,8-dihydroneopterin) using high performance liquid chromatography with fluorescence detection. These results were then compared to a control group of 20 participants with normal knee function. RESULTS:7,8-Dihydroneopterin was stable in urine over 12 h when refrigerated. Knee arthritis was associated with an increase in pre-operative neopterin (oxidative stress) and total neopterin (inflammation). The subsequent arthroplasty surgery generated a significant increase neopterin and total neopterin. Both biomarkers were reduced immediately post-operatively, before becoming elevated on the following days. There was no clear evidence of an association between initial neopterin and total neopterin levels and a patient's level of inflammation during in-hospital recovery. CONCLUSIONS:The stability of 7,8-dihydroneopterin in urine allows for its use as an inflammatory marker. Urinary neopterin and total neopterin provided a fast, non-invasive, and simple measure of oxidative stress and inflammation after knee arthroplasty.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Baxter-Parker G,Roffe L,Cross S,Frampton C,Hooper GJ,Gieseg SPdoi
10.1016/j.clinbiochem.2018.11.002subject
Has Abstractpub_date
2019-01-01 00:00:00pages
39-45eissn
0009-9120issn
1873-2933pii
S0009-9120(18)30775-6journal_volume
63pub_type
临床试验,杂志文章,多中心研究abstract:OBJECTIVE:Neonatal abstinence syndrome (NAS) is a rising concern with unknown long-term effects. It is apparent that higher cost of care, impact on the community and reduced quality of life are associated with similar etiologies (e.g., fetal alcohol syndrome). Detection of drug exposure in utero allows for earlier inte...
journal_title:Clinical biochemistry
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journal_title:Clinical biochemistry
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pub_type: 临床试验,杂志文章
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abstract::An early diagnosis is crucial for effective triage and management of patients with suspected acute myocardial infarction (AMI). Although troponin testing is the cornerstone of diagnosis, the sensitivity of this biomarker is still suboptimal at patient admission. The heart-type fatty acid binding protein (H-FABP) is an...
journal_title:Clinical biochemistry
pub_type: 杂志文章,meta分析,评审
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2008.06.016
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更新日期:2010-04-01 00:00:00
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doi:10.1016/s0009-9120(01)00218-1
更新日期:2001-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0009-9120(91)90541-l
更新日期:1991-04-01 00:00:00
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doi:10.1016/j.clinbiochem.2006.05.009
更新日期:2006-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2011.03.140
更新日期:2011-07-01 00:00:00
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更新日期:2009-03-01 00:00:00
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更新日期:2008-10-01 00:00:00
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pub_type: 杂志文章,meta分析
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pub_type: 杂志文章,评审
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更新日期:1995-06-01 00:00:00
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更新日期:2005-07-01 00:00:00
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更新日期:2019-11-01 00:00:00
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更新日期:2015-12-01 00:00:00
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更新日期:2015-11-01 00:00:00
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更新日期:2010-09-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2009.07.020
更新日期:2010-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0009-9120(01)00246-6
更新日期:2001-07-01 00:00:00
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更新日期:2011-09-01 00:00:00
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pub_type: 杂志文章
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