Maternal plasma RNA sequencing.

abstract：OBJECTIVE:We seek to identify the differentially expressed miRNAs in the clear cell subtype (ccRCC) of kidney cancer. DESIGN AND METHODS:We performed a miRNA microarray analysis to compare the miRNA expression levels between ccRCC tissues and their normal counterpart. The top dysregulated miRNAs were validated by quan...

journal_title：Clinical biochemistry

authors： Chow TF,Youssef YM,Lianidou E,Romaschin AD,Honey RJ,Stewart R,Pace KT,Yousef GM

更新日期：2010-01-01 00:00:00

abstract：OBJECTIVES:Concentrations of troponin measured with high sensitivity troponin assays are raised in a number of emergency department (ED) patients; however many are not diagnosed with acute myocardial infarction (AMI). Clinical comparisons between the early use (2h after presentation) of high sensitivity cardiac troponi...

journal_title：Clinical biochemistry

authors： Cullen L,Aldous S,Than M,Greenslade JH,Tate JR,George PM,Hammett CJ,Richards AM,Ungerer JP,Troughton RW,Brown AF,Flaws DF,Lamanna A,Pemberton CJ,Florkowski C,Pretorius CJ,Chu K,Parsonage WA

更新日期：2014-04-01 00:00:00

abstract：OBJECTIVE:To develop a tool to assess the clinical accuracy of glucose meter performance using an insulin dosing protocol to assess the frequency and extent of error in insulin dose categories. METHODS:Retrospective comparison of 1815 glucose meter and central laboratory glucose results obtained from 1698 critically i...

journal_title：Clinical biochemistry

authors： Lyon ME,Lyon OAS,Tran NK,DuBois JA,Lyon AW

更新日期：2019-08-01 00:00:00

abstract：OBJECTIVES:Non-invasive prenatal aneuploidy testing (NIPT) by next-generation sequencing of circulating cell-free DNA in maternal plasma relies on chromosomal ratio (chrratio) measurements to detect aneuploid values that depart from euploid ratios. Diagnostic performances are known to depend on the fraction of fetal DN...

journal_title：Clinical biochemistry

authors： Blais J,Giroux S,Caron A,Clément V,Dionne-Laporte A,Jouan L,Gauthier J,MacLeod T,Moore R,Parker J,Swanson L,Zhao Y,Rouleau G,Karsan A,Langlois S,Rousseau F

更新日期：2018-09-01 00:00:00

abstract：OBJECTIVES:Recently it has been suggested that serum beta-N-acetylhexosaminidase (Hex) could be a joint destruction marker in rheumatoid arthritis (RA) patients. However, a large amount of serum Hex activity has its source from platelets, and the blood platelet-count is often increased in RA, which may have masked the ...

journal_title：Clinical biochemistry

authors： Casal JA,Mera A,Pérez LF,Tutor JC

更新日期：2002-09-01 00:00:00

abstract：BACKGROUND:The p53 gene is an established tumor suppressor and an inducer of apoptosis. We here attempt to determine whether the putative anticarcinogenic properties attributed to red wine and its polyphenolic constituents depend, at least in part, upon their ability to modulate p53 expression in cancer cells. METHODS...

journal_title：Clinical biochemistry

authors： Soleas GJ,Goldberg DM,Grass L,Levesque M,Diamandis EP

更新日期：2001-07-01 00:00:00

abstract：OBJECTIVE:To evaluate the potential diagnostic value of apolipoprotein E (ApoE) measurements in pediatric patients with invasive bacterial infections. DESIGN AND METHOD:A total of 185 pediatric patients were enrolled in this study, including 94 patients with confirmable infections and 91 patients without confirmable i...

journal_title：Clinical biochemistry

authors： Wang C,Wang Y,Wang A,Fu P,Yang Y

更新日期：2012-02-01 00:00:00

abstract：OBJECTIVES:Oxidative stress increases postmenopausally, an effect attributed to aging. Increase in body weight generally accompanies menopause. Obesity per se also seems to increase oxidative stress. The question is whether postmenopausal increase in oxidative stress is related to body weight, or is only a function of ...

journal_title：Clinical biochemistry

authors： Mittal PC,Kant R

更新日期：2009-07-01 00:00:00

abstract：:A fluorescence polarization (FP) technique was applied to the assay of lysozyme activity in human urine. In this assay system, reaction between enzyme and fluorescein-labelled substrate produced a decrease in the fluorescein polarization (P) value. Enzyme activity and rate of decrease of P value exhibited a hyperbolic...

journal_title：Clinical biochemistry

authors： Miura H

更新日期：1985-02-01 00:00:00

abstract：OBJECTIVE:Quantitative sweat production and -ionic composition in Essential Hyperhidrosis (EH), and the effects of T2-T3 thoracoscopic sympathicolysis (TS) hereon, are unknown. Standardised pilocarpine iontophoresis sweat tests were performed before and after TS in order to study these issues. DESIGN AND METHODS:Piloc...

journal_title：Clinical biochemistry

authors： Noppen M,Sevens C,Vincken WG

更新日期：1997-03-01 00:00:00

abstract：OBJECTIVES:To investigate the possible association between functional variant Ala-9Val in the MnSOD gene and asthma in the case-control study comprising 626 Caucasian subjects. METHODS:MnSOD genotypes were determined by PCR with subsequent restriction analysis by the BsaWI enzyme. RESULTS:Significant differences in a...

journal_title：Clinical biochemistry

authors： Holla LI,Kankova K,Vasku A

更新日期：2006-03-01 00:00:00

abstract：:Drug therapy may produce toxicity. Patient individuality or drug interactions account for many cases of poisoning, but other factors such as genetic predisposition, drug contamination or human error are also known causes. Examples of various types of drug poisoning are given, illustrating the role of the clinical chem...

journal_title：Clinical biochemistry

authors： Stewart MJ

更新日期：1990-02-01 00:00:00

abstract：:Iodine deficiency (ID) is associated with increased prevalence of goiter, increased risk for neurodevelopmental disorders, and is the world's leading cause of intellectual deficits. Iodine nutritional status of a population is assessed by measurements of urinary iodine concentrations which are also used to define, ind...

journal_title：Clinical biochemistry

authors： Soldin OP

更新日期：2002-11-01 00:00:00

abstract：OBJECTIVES:To examine the diagnostic validity of bone alkaline phosphatase (BAP) in serum (a marker of bone formation) and of urinary excretion of pyridinium cross-links (PYR) (a marker of bone resorption) for detection of bone metastases. DESIGN AND METHODS:Two heterogeneous immunoassays were employed for determinati...

journal_title：Clinical biochemistry

authors： Withold W,Georgescu G,Khakzad H,Vosberg H,Mueller-Gaertner HW,Reinauer H

更新日期：1995-10-01 00:00:00

abstract：OBJECTIVE:The detection of possible correlation between ribosomal protein RPS27A and multifunctional YB-1 expression in hepatocellular carcinoma (HCC). DESIGN AND METHODS:Tissue microarray slides containing totally 80 cores with 19 tissues of HCC, 1 tissue of hepatocholangiocarcinoma, 10 tissues of liver cirrhosis and...

journal_title：Clinical biochemistry

authors： Gunasekaran VP,Ganeshan M

更新日期：2014-09-01 00:00:00

abstract：:Immunoglobulin levels are elevated in the acquired immune deficiency syndrome (AIDS) and one class, IgA, has been suggested as a marker of disease progression. IgA is composed of two subclasses, IgA1 and IgA2; circulating IgA1 is roughly 85% of the total in healthy individuals. We have attempted to determine if the su...

journal_title：Clinical biochemistry

authors： Cassulis P,Farhangfar R,DeBari VA

更新日期：1991-06-01 00:00:00

abstract：OBJECTIVES:To determine the serum ratio of holo-ceruloplasmin to total ceruloplasmin in patients with Wilson's disease (WD), we identified apo- and holo-forms of serum ceruloplasmin with native-PAGE electrophoresis. DESIGN AND METHODS:Serum obtained from nine patients with Wilson's disease was subjected to native-PAGE...

journal_title：Clinical biochemistry

authors： Hirano K,Ogihara T,Ogihara H,Hiroi M,Hasegawa M,Tamai H

更新日期：2005-01-01 00:00:00

abstract：:This review deals with the application of time-resolved fluorescence spectroscopy in the field of immunoassay. The advantages of time-resolved fluorescence over conventional fluorometry are discussed and it is explained why the fluorescent lanthanide chelates, and especially the Eu3+ chelates, are the labels of choice...

journal_title：Clinical biochemistry

authors： Diamandis EP

更新日期：1988-06-01 00:00:00

abstract：OBJECTIVES:To investigate the association between oxidative stress and certain trace elements in the blood of breast cancer patients. DESIGN AND METHODS:Malondialdehyde (MDA) was measured in serum of patients with breast cancer (n = 35) and controls (n = 35) by high performance liquid chromatography. Trace elements we...

journal_title：Clinical biochemistry

authors： Huang YL,Sheu JY,Lin TH

更新日期：1999-03-01 00:00:00

abstract：OBJECTIVES:An increase in oxidative stress may contribute to the development of oxidative protein damage in the aging rat skeletal muscle. Our aim was to reveal protein carbonyl (PCO), advanced oxidation protein products (AOPP), a novel marker of oxidative stress, and protein thiol (P-SH) levels as markers of protein o...

journal_title：Clinical biochemistry

authors： Cakatay U,Telci A,Kayali R,Tekeli F,Akçay T,Sivas A

更新日期：2003-02-01 00:00:00

abstract：OBJECTIVE:This article will review the advancements and new developments being made in (1) advanced computers, (2) microtechnology, (3) advanced immunodiagnostics, (4) neural networks, and (5) molecular biology. The influence of these technologies and their products on clinical laboratories is also discussed. CONCLUSI...

journal_title：Clinical biochemistry

authors： Burtis CA

更新日期：1995-06-01 00:00:00

abstract：OBJECTIVES:Hepcidin is the main regulator of systemic iron homeostasis and its expression is modulated by iron status, hypoxia, erythroid factors and inflammation. The aim of our study was to examine a relationship between level of hepcidin and iron status, erythropoietic activity, hypoxia and inflammation in exacerbat...

journal_title：Clinical biochemistry

authors： Tandara L,Grubisic TZ,Ivan G,Jurisic Z,Tandara M,Gugo K,Mladinov S,Salamunic I

更新日期：2015-12-01 00:00:00

abstract：OBJECTIVES:Presence of residual blood is a common problem in cerebrospinal fluid (CSF) diagnostics of ventriculitis. We hypothesised that neutrophil granulocytes in infected, blood-containing CSF lose CD62L expression. Therefore CD62L expression on neutrophils may present a complementary marker to distinguish between p...

journal_title：Clinical biochemistry

authors： Boer K,Vogelsang H,Deufel T,Pfister W,Kiehntopf M

更新日期：2010-11-01 00:00:00

abstract：OBJECTIVES:We have previously identified three minor immunophilins of molecular weights 37 kDa, 14 kDa, and 5-8 kDa capable of binding tacrolimus and sirolimus. DESIGN AND METHODS:When tested against pure preparations of five sirolimus metabolites, the 14 kDa protein had almost no cross-reactivity, the 37 kDa protein ...

journal_title：Clinical biochemistry

authors： Davis DL,Murthy JN,Gallant-Haidner H,Yatscoff RW,Soldin SJ

更新日期：2000-02-01 00:00:00

abstract：OBJECTIVES:The fibroblast growth factor receptor 3 gene (FGFR3) plays a critical role in cartilage growth-plate differentiation and bony development. It has been shown that 97% of patients with achondroplasia have a G to A transition mutation at position 1138 (c.1138 G>A) of codon 380 of the FGFR3 gene. DESIGN AND MET...

journal_title：Clinical biochemistry

authors： Hung CC,Lee CN,Chang CH,Jong YJ,Chen CP,Hsieh WS,Su YN,Lin WL

更新日期：2008-02-01 00:00:00

abstract：OBJECTIVES:Oxidative stress plays a role in the pathogenesis in patients with HCV infection. The objective of this study was to evaluate oxidant and antioxidant biomarkers in patients with HCV. DESIGN AND METHODS:Serum malonaldehyde (MDA) and nitric oxide (NO) levels and the activities of myeloperoxidase (MPO), aryles...

journal_title：Clinical biochemistry

authors： Ali EM,Shehata HH,Ali-Labib R,Esmail Zahra LM

更新日期：2009-09-01 00:00:00

abstract：OBJECTIVES:To develop and validate a novel genotyping approach, named infrared Fluorescence Allele Specific Hybridization (iFLASH), which combines the principles of allele specific oligonucleotide (ASO) hybridization with the advanced possibilities of infrared imaging. DESIGN AND METHODS:As an example, we genotyped th...

journal_title：Clinical biochemistry

authors： van Himbergen TM,Voorbij HA,Barendrecht AD,van Rijn BB,Brambilla R,van Tits LJ,Roest M

更新日期：2006-07-01 00:00:00

abstract：OBJECTIVES:To determine salivary cortisol reference intervals in a healthy adult population, at 6 different time points during a 24-hour (h) period. METHODS:In a prospective study, salivary cortisol concentrations were measured upon waking, one-hour post-waking and at specific times of the day: at 12 h00, 16 h00, 20 h...

journal_title：Clinical biochemistry

authors： Gagnon N,Fréchette I,Mallet PL,Dubé J,Houde G,Fink GD

更新日期：2018-04-01 00:00:00

abstract：:An affinity chromatographic method (AC) was compared with ion exchange chromatography (IEC) for determination of glycated hemoglobin (gHb). In vitro, increasing glucose concentration produced no change on AC-determined gHb. gHb measurement by AC was highly correlated with mean blood glucose (r = 0.96). Mean values of ...

journal_title：Clinical biochemistry

authors： Wettre S,von Schenck H

更新日期：1986-12-01 00:00:00

abstract：BACKGROUND:With the advent of the new high-sensitivity troponin assays, it is becoming critical to measure troponin accurately to low concentrations. To ensure assay performance is acceptable, appropriate QC must be run. METHODS:In addition to the routine use of commercial QC material, we prepared pools of human QC ma...

journal_title：Clinical biochemistry

authors： Hickman PE,Koerbin G,Badrick T,Oakman C,Potter JM

更新日期：2018-08-01 00:00:00