当前位置： SCI文献检索 > CLINICAL BIOCHEMISTRY期刊下所有文献 > Involvement of oxidative and nitrosative stress in promoting retinal vasculitis in patients with Eales' disease.

Involvement of oxidative and nitrosative stress in promoting retinal vasculitis in patients with Eales' disease.

Abstract：

OBJECTIVES:Eales' disease (ED) is an idiopathic retinal vasculitis condition, which affects retina of young adult males. The histopathological hallmark in ED is the adhesion of leukocytes to the endothelium and the infiltration of these cells into the retinal parenchyma. Phagocyte generated free radicals have been implicated in mediating tissue damage associated with various inflammatory vasculopathies. In the present study, we have investigated the possible role of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in causing retinal tissue damage in ED. DESIGN AND METHODS:35 patients with ED and 20 healthy control subjects were included in the study. Monocytes (MC) were separated from peripheral blood of the respective study participants. Inducible nitric oxide synthase (iNOS) protein expression was assessed using Western blot and 3 nitrotyrosine (3NTYR) by reversed phase high performance liquid chromatography (RP HPLC). Thiobarbituric acid reactive substances (TBARS) were determined by measuring malondialdehyde (MDA) formed. Superoxide dismutase (SOD) activity was assayed based on the ability of SOD to inhibit auto-oxidation of epinephrine. Iron, copper and zinc content were determined using Atomic Absorption Spectrophotometer. Immunolocalization of iNOS and 3NTYR was performed on the surgically excised epiretinal membranes (ERM) from patients with ED. RESULTS:There was a significant increase in the expression of iNOS, as well as 3NTYR accumulation, diminished SOD activity, elevated lipid peroxides, iron, copper and decreased zinc content in the MC of patients with ED when compared with healthy control subjects. The elevated levels of ROS and RNS products correlated with diminished antioxidant status in patients with ED. Strong immunoreactivity for iNOS and 3NTYR was observed in inflammatory cells and endothelial cells in ERM obtained from patients with ED. CONCLUSIONS:Our findings from this study clearly reveal the involvement of RNS and ROS in the development of retinal vasculitis in ED. Based on our present study and earlier studies we confirm the role of free radicals in mediating retinal tissue damage in ED. Hence we believe selective inhibition of iNOS or supplementation with antioxidants vitamin E and C might be beneficial in controlling retinal vasculitis in patients with ED.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Rajesh M,Sulochana KN,Punitham R,Biswas J,Lakshmi S,Ramakrishnan S

doi

10.1016/s0009-9120(03)00058-4

subject

Has Abstract

pub_date

2003-07-01 00:00:00

pages

377-85

issue

5

eissn

0009-9120

issn

1873-2933

pii

S0009912003000584

journal_volume

36

pub_type

杂志文章

相关文献

CLINICAL BIOCHEMISTRY文献大全
  • Evaluation and risk stratification of acute coronary syndromes using a low cut-off level of cardiac troponin T, combined with CK-MB mass determination.

    abstract：OBJECTIVES:To study the usefulness of combined cardiac Troponin T (cTnT) and CK-MB mass determinations in risk stratification of acute coronary syndromes. DESIGN AND METHODS:Blood samples for cTnT and CK-MB mass were collected at arrival and 4, 8, and 12-24 later in 301 consecutive patients with recent acute chest pai...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2003.12.003

    authors： Solymoss BC,Bourassa MG,Fortier A,Théroux P

    更新日期：2004-04-01 00:00:00

  • Low normal thyroid function as a determinant of increased large very low density lipoprotein particles.

    abstract：OBJECTIVES:Low-normal thyroid function may relate to increases in plasma cholesterol and triglycerides, but effects on lipoprotein subfractions are largely unknown. Associations of alterations in lipoprotein metabolism and functionality with low-normal thyroid function could be more pronounced in Type 2 diabetes mellit...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2015.01.015

    authors： van Tienhoven-Wind L,Dullaart RP

    更新日期：2015-05-01 00:00:00

  • Evaluation of predictive value of pleural CEA in patients with pleural effusions and histological findings: A prospective study and literature review.

    abstract：OBJECTIVES:Pleural effusion recognizes heterogeneous etiology and pathogenesis and requires invasive diagnostic procedures. Usually, after pleural fluid analysis, 30-50% of patients with malignant pleural effusion exhibit negative pleural cytology, and the sensitivity of image-guided pleural needle-aspiration biopsy ra...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.clinbiochem.2016.08.006

    authors： Tozzoli R,Basso SM,D'Aurizio F,Metus P,Lumachi F

    更新日期：2016-11-01 00:00:00

  • Association between positive fecal occult blood test and diabetes in a population undergoing health screening.

    abstract：BACKGROUND:Numerous etiologies are implicated in the complications of diabetes. A link between diabetes and the predisposition to certain cancers, including colon cancer, has been established during the last decade. However, the relationship between fecal occult blood test (FOBT), a screening method used principally fo...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2016.10.005

    authors： Nakajima K,Suwa K

    更新日期：2017-01-01 00:00:00

  • Influences of sleep and the circadian rhythm on iron-status indices.

    abstract：OBJECTIVES:The aim was to study the influence of sampling time, food intake and sleep on tests used to screen for and monitor conditions of iron deficiency and overload. DESIGN AND METHODS:The 24 h variations of iron, transferrin, transferrin saturation (TS) and ferritin were studied in seven healthy individuals durin...

    journal_title：Clinical biochemistry

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.clinbiochem.2010.08.023

    authors： Ridefelt P,Larsson A,Rehman JU,Axelsson J

    更新日期：2010-11-01 00:00:00

  • Urinary beta-luteinizing hormone and beta-follicle stimulating hormone immunoenzymometric assays for population research.

    abstract：OBJECTIVE:We developed assays for measurement of urinary betaLH and betaFSH under collection and storage conditions typical of non-clinical research settings. DESIGN AND METHODS:IEMAs for free betaLH and total betaFSH were validated by standard methods. Stability of urinary betaLH and betaFSH was tested across freeze-...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2006.08.009

    authors： Brindle E,Miller RC,Shofer JB,Klein NA,Soules MR,O'Connor KA

    更新日期：2006-11-01 00:00:00

  • Association of serum bilirubin levels with albuminuria in patients with essential hypertension.

    abstract：OBJECTIVES:This study was undertaken to evaluate the relationship between serum bilirubin concentrations and the degree of urinary albumin excretion in hypertensive patients. DESIGN AND METHODS:A total of 120 hypertensive subjects were enrolled, in which 80 (67%) with normoalbuminuria (albumin excretion rate [AER] of ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2011.03.137

    authors： Huang SS,Huang PH,Chiang KH,Chen JW,Lin SJ

    更新日期：2011-07-01 00:00:00

  • Evaluation of the Dupont aca bromocresol purple albumin method.

    abstract：:The bromocresol purple (BCP) albumin method on the DuPont aca was evaluated by determining the accuracy and precision of the method. A split sample comparison was performed against the electroimmunoassay (EIA) method as well as the bromocresol green (BCG) method on the DuPont aca and the Technicon SMA 12/60. The BCP m...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(83)90490-3

    authors： Krahn J

    更新日期：1983-04-01 00:00:00

  • Medical errors: impact on clinical laboratories and other critical areas.

    abstract：:The Institute of Medicine (IOM) report (1999) stated that the prevalence of medical errors is high in today's health care system. Some specialties in health care are more risky than others. A varying blunder/error rate of 0.1-9.3% in clinical diagnostic laboratories has been reported in the literature. Many of these e...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.clinbiochem.2004.08.009

    authors： Kalra J

    更新日期：2004-12-01 00:00:00

  • Association of glycated albumin to HbA1c ratio with diabetic retinopathy but not diabetic nephropathy in patients with type 2 diabetes.

    abstract：OBJECTIVES:The ratio of glycated albumin to HbA1c (GA/HbA1c ratio) is a known indicator that reflects fluctuations in plasma glucose. In this study, the association of the GA/HbA1c ratio to diabetic nephropathy and diabetic retinopathy in patients with type 2 diabetes was investigated. DESIGN AND METHODS:Among patient...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2016.11.032

    authors： Umayahara Y,Fujita Y,Watanabe H,Kasai N,Fujiki N,Hatazaki M,Koga M

    更新日期：2017-04-01 00:00:00

  • Serum cystatin C as a better marker of vancomycin clearance than serum creatinine in elderly patients.

    abstract：OBJECTIVES:The purpose of this study is to assess the usefulness of the concentration of cystatin C (Cys-C) in serum for predicting the clearance of vancomycin (CLvcm) compared with the serum concentration of creatinine (SCr) in the elderly. METHODS:Thirty-nine serum samples were obtained from 24 elderly patients (65 ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2007.01.008

    authors： Okamoto G,Sakamoto T,Kimura M,Ukishima Y,Sonoda A,Mori N,Kato Y,Maeda T,Kagawa Y

    更新日期：2007-04-01 00:00:00

  • Comparison of gas-liquid chromatography and EMIT assay for serum valproic acid.

    abstract：:We describe a simple direct extraction method for the gas-liquid chromatography determination of serum valproic acid. The working range for the assay is 2-180 mg/L and our within-run precision was 5.8 and 4.3% at the 40 and 90 mg/L concentrations respectively. Hemolyzed and lipemic sera as well as samples from patient...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(81)90772-4

    authors： Leroux M,Budnik D,Hall K,Irvine-Meek J,Otten N,Seshia S

    更新日期：1981-04-01 00:00:00

  • The effects of bisphenol A (BPA) exposure on fat mass and serum leptin concentrations have no impact on bone mineral densities in non-obese premenopausal women.

    abstract：OBJECTIVES:Bisphenol A (BPA) exposure may promote obesity, but its effect on bone mineral density (BMD) has not been reported in humans. We aimed to examine the relationships between BPA exposure, body composition, serum estradiol, leptin, osteocalcin levels and BMDs in healthy premenopausal women. DESIGN AND METHODS:...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2012.08.024

    authors： Zhao HY,Bi YF,Ma LY,Zhao L,Wang TG,Zhang LZ,Tao B,Sun LH,Zhao YJ,Wang WQ,Li XY,Xu MY,Chen JL,Ning G,Liu JM

    更新日期：2012-12-01 00:00:00

  • Determination of serum vitamins 25-OH-D2 and 25-OH-D3 with liquid chromatography-tandem mass spectrometry using atmospheric pressure chemical ionization or electrospray source and core-shell or sub-2 μm particle columns: a comparative study.

    abstract：OBJECTIVE:Develop a simple LC-MS-MS method for determination of 25-hydroxymetabolites of vitamin D2 and D3 in serum. DESIGN AND METHODS:0.1 mL serum was mixed with 0.3 mL deproteinizing mixture containing two deuterated internal standards. Two LC-MS-MS systems were used: Waters Premier with an ESI source and a Hypersi...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2011.08.1134

    authors： Bogusz MJ,Al Enazi E,Tahtamoni M,Jawaad JA,Al Tufail M

    更新日期：2011-11-01 00:00:00

  • Comparative performance of two methods that assess the quality of international normalized ratio measures.

    abstract：OBJECTIVES:Two methods, Petersen's error grid analysis and Shermock's method to detect clinically important differences, were recently developed to advance the assessment of analytic performance of point-of-care INR devices. Both methods predict when alternate INR measures lead to different clinical decisions. Our goal...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2012.01.032

    authors： Shermock KM,Tandon S,Sorgen PJ,Lavallee DC,Clarke W,Streiff MB

    更新日期：2012-05-01 00:00:00

  • Inherited factor V deficient neonate with galactosaemia.

    abstract：OBJECTIVES:Reporting a case of inherited factor V deficiency and galactosemia. METHODS:A neonate was admitted with hematoma, jaundice, splenomegaly, diarrhea, anemia, abdominal ascites and bilateral cataracts that diagnosis of galactosaemia and factor V deficiency was established. RESULTS:Coinheritance of both coagul...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.clinbiochem.2011.12.024

    authors： Mansouritorghabeh H,Sharifi-Hoseini MR,Shahroudian M

    更新日期：2012-03-01 00:00:00

  • Method performance and clinical workflow outcomes associated with meconium and umbilical cord toxicology testing.

    abstract：OBJECTIVE:Neonatal abstinence syndrome (NAS) is a rising concern with unknown long-term effects. It is apparent that higher cost of care, impact on the community and reduced quality of life are associated with similar etiologies (e.g., fetal alcohol syndrome). Detection of drug exposure in utero allows for earlier inte...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2017.09.016

    authors： Labardee RM,Swartzwelder JR,Gebhardt KE,Pardi JA,Dawsey AC,Brent Dixon R,Cotten SW

    更新日期：2017-12-01 00:00:00

  • The Italian pilot external quality assessment program for cystic fibrosis sweat test.

    abstract：OBJECTIVES:Sweat chloride test is the gold standard test for cystic fibrosis (CF) diagnosis. In 2014 the Istituto Superiore di Sanità established the Italian pilot external quality assessment program for CF sweat test (IEQA-ST). DESIGN AND METHODS:Ten laboratories, included among the 33 Italian CF Referral Centers, we...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2015.12.014

    authors： Salvatore M,Floridia G,Amato A,Censi F,Carta C,de Stefano MC,Ferrari G,Tosto F,Capoluongo E,Caruso U,Castaldo G,Cirilli N,Corbetta C,Padoan R,Raia V,Taruscio D

    更新日期：2016-05-01 00:00:00

  • Oxidative enzymes of polymorphonuclear leucocytes and plasma fibrinogen, ceruloplasmin, and copper levels in Behçet's disease.

    abstract：:This study was performed to investigate the antioxidant mechanisms of polymorphonuclear leucocytes (PMN) in active stage of Behçet's Disease. PMN activities of myeloperoxidase (p < 0.02), superoxide dismutase (p < 0.001), catalase (p < 0.005), and glutathione peroxidase (p < 0.005) were significantly lower in the pati...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/0009-9120(94)90046-9

    authors： Doğan P,Tanrikulu G,Soyuer U,Köse K

    更新日期：1994-10-01 00:00:00

  • Evaluation and assessment of high sensitivity thyrotropin methods as an index for thyroid function.

    abstract：:We report the evaluation of four new commercially available sensitive assay kits for determination of thyrotropin (TSH) and their clinical utility in normal subjects and patients with thyroidal and non-thyroidal illnesses. The sensitivity for the reliable detection of serum TSH by these methods ranged from 0.1-0.4 mU/...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(87)80077-2

    authors： Kalra J,Laxdal VA,Walker SD,Massey KL

    更新日期：1987-10-01 00:00:00

  • A survey to identify potential outcome indicators for a hospital blood glucose monitoring program.

    abstract：OBJECTIVE:A survey of operators of a bedside blood glucose monitoring (BGM) program at a tertiary health care institution was performed to identify potential outcome indicators for our quality assurance program. DESIGN AND METHODS:170 surveys were randomly distributed to each nursing unit. The survey consisted of 20 q...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(98)00020-4

    authors： Collier CP,Houlden RL,Gleeson KA,Patrzykat A,Rhymer SL

    更新日期：1998-06-01 00:00:00

  • Comparative evaluation of the chemopreventive efficacy of green and black tea polyphenols in the hamster buccal pouch carcinogenesis model.

    abstract：OBJECTIVES:To evaluate the comparative chemopreventive efficacy of green tea polyphenols (Polyphenon-E) and black tea polyphenols (Polyphenon-B) on 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis. DESIGN AND METHODS:Hamsters were divided into 6 groups. Animals in group 1 served ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2005.06.011

    authors： Chandra Mohan KV,Hara Y,Abraham SK,Nagini S

    更新日期：2005-10-01 00:00:00

  • Unusually high creatine kinase BB isoenzyme and study of lactate dehydrogenase pattern in metastatic adenocarcinoma of the prostate.

    abstract：:We report a case of metastatic adenocarcinoma of the prostate with widespread bony and hepatic involvement having extremely high BB isoenzyme of creatine kinase in serum (2405 U/liter). The serum LD5/LD1 ratio is greater than one, with total LD activity more than twenty-five fold the normal value. The homogenate of pr...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(80)90502-0

    authors： Sandhu RS,Conover RE

    更新日期：1980-02-01 00:00:00

  • Transthyretin as a marker to predict outcome in critically ill patients.

    abstract：BACKGROUND:A determination of serum Transthyretin (TTR, Prealbumin) level is an objective method of assessing protein catabolic loss of severely ill patients and numerous studies have shown that TTR levels correlate with patient outcomes of non-critically ill patients. We evaluated whether TTR level correlates with the...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2008.06.016

    authors： Devakonda A,George L,Raoof S,Esan A,Saleh A,Bernstein LH

    更新日期：2008-10-01 00:00:00

  • Quantitative analysis of trace elements in human blood and plasma by energy dispersive X-ray fluorescence.

    abstract：:A photon excitation, secondary target x-ray fluorescence system was used to measure concentrations of Cu, Zn, Br and Rb in whole blood and plasma of healthy adults. The samples preparation method and calculated limits of detection of the technique are reported. Correlation statistics for elemental concentrations have ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(77)91740-4

    authors： Stump IG,Carruthers J,D'Auria JM

    更新日期：1977-06-01 00:00:00

  • Association of serum adiponectin with diabetic microvascular complications among south Indian type 2 diabetic subjects - (CURES-133).

    abstract：OBJECTIVES:To assess the association of serum adiponectin and microvascular complications of diabetes in an urban south Indian type 2 diabetic population. DESIGN AND METHODS:Diabetic subjects [n=487] were included from Chennai Urban Rural Epidemiology Study (CURES). Four-field stereo retinal color photography was done...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2014.10.009

    authors： Pradeepa R,Surendar J,Indulekha K,Chella S,Anjana RM,Mohan V

    更新日期：2015-01-01 00:00:00

  • Background hCG in non-pregnant individuals: need for more sensitive point-of-care and over-the-counter pregnancy tests.

    abstract：BACKGROUND:Currently, 20 IU/L is the accepted sensitivity of over-the-counter (OTC) hCG pregnancy tests, and the only sensitivity permitted for point of care (POC) tests. These sensitivity limitations cause significant numbers of false-negative tests, which mislead women and cause them to avoid precautions needed to av...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2008.09.107

    authors： Cole LA,Ladner DG

    更新日期：2009-02-01 00:00:00

  • Ethanolaminuria: a non-specific laboratory finding in the seriously ill infant.

    abstract：:Ethanolamine is a compound that is frequently seen in urinary amino acid analysis. Although there is a single report of increased ethanolamine excretion associated with a distinctive storage disease in two siblings, the significance of ethanolaminuria is not known. We measured urinary ethanolamine/creatinine ratios in...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(88)80084-5

    authors： Cole DE,Farag S,Dooley KC

    更新日期：1988-10-01 00:00:00

  • Clinical and biochemical characteristics of patients with urea cycle disorders in a developing country.

    abstract：OBJECTIVES:To report the clinical and laboratory characteristics of urea cycle disorder (UCD) patients at a tertiary care center in a developing country. DESIGN AND METHODS:Retrospective study of clinical and laboratory data of UCD patients. RESULTS:Thirty-seven UCD patients were studied, 31 symptomatic (high risk) p...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2009.12.004

    authors： Ibarra-González I,Fernández-Lainez C,Vela-Amieva M

    更新日期：2010-03-01 00:00:00

  • Single-strand conformational polymorphisms (SSCP): studies of the genetic polymorphisms of exon 4 of apolipoprotein C III.

    abstract：OBJECTIVE:We used single-strand conformational polymorphism (SSCP). To screen for mutations/polymorphisms in exon 4 of the apolipoprotein C III in 45 patients with hypertriglyceridemia and 46 control individuals, single-strand conformational polymorphism was investigated using restriction endonuclease and amplification...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/0009-9120(94)00083-8

    authors： Beheshti I,Hanson NQ,Copeland KR,Garg U,Tsai MY

    更新日期：1995-06-01 00:00:00