Problems with the estimation of urine protein by automated assays.

Abstract:

OBJECTIVES:Most clinical laboratories replaced their manual precipitation techniques for the determination of urinary protein with automated dye binding assays or benzethonium chloride-turbidimetric assays. Few studies have validated these assays for the measurement of urinary proteins in the normal range. DESIGN AND METHODS:This study compares four automated assays for the measurement of urinary protein to a manual Ponceau S/TCA precipitation assay. We evaluated the linearity, the precision, the analytical sensitivity, the accuracy and the recovery of different proteins for each assay. RESULTS:All assays showed good linearity with the theoretical concentration of albumin present in the sample. The coefficient of variation was below 10% at a concentration of 0.142 g/L. However, the manual Ponceau S/TCA assay demonstrated superior analytical sensitivity. Accuracy determinations showed a variable positive bias and poor correlations at concentrations below 0.1 g/L when compared to the Ponceau S/TCA assay. Small molecular weight peptides particularly affected the pyrogallol red assays but other urinary components also interfered with the automated assays. CONCLUSIONS:Most automated assays show high imprecision and poor accuracy for the measurement of urinary protein in the normal range. The Ponceau S/TCA offers a precise and accurate manual alternative to these automated assays.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Dube J,Girouard J,Leclerc P,Douville P

doi

10.1016/j.clinbiochem.2004.12.010

subject

Has Abstract

pub_date

2005-05-01 00:00:00

pages

479-85

issue

5

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(04)00365-0

journal_volume

38

pub_type

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