Abstract:
OBJECTIVES:Most clinical laboratories replaced their manual precipitation techniques for the determination of urinary protein with automated dye binding assays or benzethonium chloride-turbidimetric assays. Few studies have validated these assays for the measurement of urinary proteins in the normal range. DESIGN AND METHODS:This study compares four automated assays for the measurement of urinary protein to a manual Ponceau S/TCA precipitation assay. We evaluated the linearity, the precision, the analytical sensitivity, the accuracy and the recovery of different proteins for each assay. RESULTS:All assays showed good linearity with the theoretical concentration of albumin present in the sample. The coefficient of variation was below 10% at a concentration of 0.142 g/L. However, the manual Ponceau S/TCA assay demonstrated superior analytical sensitivity. Accuracy determinations showed a variable positive bias and poor correlations at concentrations below 0.1 g/L when compared to the Ponceau S/TCA assay. Small molecular weight peptides particularly affected the pyrogallol red assays but other urinary components also interfered with the automated assays. CONCLUSIONS:Most automated assays show high imprecision and poor accuracy for the measurement of urinary protein in the normal range. The Ponceau S/TCA offers a precise and accurate manual alternative to these automated assays.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Dube J,Girouard J,Leclerc P,Douville Pdoi
10.1016/j.clinbiochem.2004.12.010subject
Has Abstractpub_date
2005-05-01 00:00:00pages
479-85issue
5eissn
0009-9120issn
1873-2933pii
S0009-9120(04)00365-0journal_volume
38pub_type
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journal_title:Clinical biochemistry
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journal_title:Clinical biochemistry
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