Comparison of immunoturbidimetric and immunonephelometric assays for specific proteins.

Abstract:

:Immunoturbidimetric assays for specific proteins are available on "open system" clinical chemistry analyzers. The analytical performance of nine immunoturbidimetric specific protein assays (C3, C4, CRP, Haptoglobin, IgA, IgG, IgM, RF, and Transferrin) was compared to immunonephelometry. Testing was performed on the Abbott ARCHITECT ci8200 and the Dade Behring BNII nephelometer and evaluated for precision, linearity, limit of detection, prozone phenomenon, method comparison, workflow, and proficiency testing survey comparison. Immunoturbidimetric assays performance was satisfactory for total precision, linearity, limit of detection and the prozone effect was not observed. Method comparison was acceptable for the immunoglobulins, CRP and transferrin but less favorable for the other assays, likely due to methodology and antibody specificity differences. Immunourbidimetric specific protein assays allow for efficient test consolidation on a general purpose clinical chemistry analyzer.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Mali B,Armbruster D,Serediak E,Ottenbreit T

doi

10.1016/j.clinbiochem.2009.06.016

subject

Has Abstract

pub_date

2009-10-01 00:00:00

pages

1568-71

issue

15

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(09)00277-X

journal_volume

42

pub_type

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