Long-term stability of biochemical markers in pediatric serum specimens stored at -80 °C: a CALIPER Substudy.

Abstract:

OBJECTIVES:Pediatric serum samples collected from healthy children in the CALIPER (Canadian Laboratory Initiative in Pediatric Reference Interval) project are stored at -80 °C for various periods of time. This study aimed to determine the stability of chemistry, protein, and hormone analytes under these conditions. DESIGN AND METHODS:Serum samples collected from children of 0-18 years of age attending outpatient clinics were pooled into a single pool or into age-group specific pools. Following baseline measurement, each pool was aliquoted and kept frozen at -80 °C until analysis. Samples were analyzed for 57 biochemical markers at monthly intervals over a 10-13 month period and each aliquot was subject to one freeze-thaw cycle before analysis. The analysis was performed on VITROS Chemistry System, COBAS INTEGRA 400 Plus and IMMULITE 2500. Values obtained at monthly intervals were compared to baseline measurements and examined for trends over time. RESULTS:A majority of analytes measured in this study showed no significant time-dependent change relative to baseline or trend over time after up to 13 months of storage. PTH showed up to 27.2% decline after 10 months of storage with most of the decline evident after 2 months. Most analytes showed variability over time, which is thought to reflect assay variability rather than changes in analyte stability. CONCLUSIONS:The study shows stability for a majority of analytes stored in serum at -80 °C after up to 13 months of storage. Samples do not require immediate testing for reference interval determination for the selected analytes with possible exception of PTH.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Brinc D,Chan MK,Venner AA,Pasic MD,Colantonio D,Kyriakopolou L,Adeli K

doi

10.1016/j.clinbiochem.2012.03.029

subject

Has Abstract

pub_date

2012-07-01 00:00:00

pages

816-26

issue

10-11

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(12)00160-9

journal_volume

45

pub_type

杂志文章
  • Monoclonal antibody technology for cyclosporine monitoring.

    abstract::Monitoring blood levels of Cyclosporine (CsA) has been the basis for adjusting individual dosage regimens in the clinic. Radioimmunoassays using polyclonal antisera reacted with CsA and some CsA metabolites, leading to overestimation when compared with high-performance liquid chromatographic measurements of CsA. Monoc...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/0009-9120(91)90147-7

    authors: Quesniaux VF

    更新日期:1991-02-01 00:00:00

  • IDH1 and IDH2 mutations in patients with acute myeloid leukemia: Suitable targets for minimal residual disease monitoring?

    abstract:OBJECTIVES:Molecular screening plays a major role in prognostic categorization and subsequent definition of treatment strategies for acute myeloid leukemia. The possibility of using IDH1/2 mutations as a marker for the monitoring of minimal residual disease (MRD) is still under investigation and remains unclear. METHO...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2018.08.012

    authors: Petrova L,Vrbacky F,Lanska M,Zavrelova A,Zak P,Hrochova K

    更新日期:2018-11-01 00:00:00

  • Simultaneous determination of glucose, triglycerides, urea, cholesterol, albumin and total protein in human plasma by Fourier transform infrared spectroscopy: direct clinical biochemistry without reagents.

    abstract:OBJECTIVE:Direct measurement of chemical constituents in complex biologic matrices without the use of analyte specific reagents could be a step forward toward the simplification of clinical biochemistry. Problems related to reagents such as production errors, improper handling, and lot-to-lot variations would be elimin...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2014.05.064

    authors: Jessen TE,Höskuldsson AT,Bjerrum PJ,Verder H,Sørensen L,Bratholm PS,Christensen B,Jensen LS,Jensen MA

    更新日期:2014-09-01 00:00:00

  • A physiological approach to jaundice.

    abstract::An understanding of the production, transport, hepatic uptake, biotransformation and excretion of bile pigments is necessary for the rational investigation of a patient presenting with jaundice. These aspects are reviewed, and at each step of bilirubin metabolism the clinical abnormalities giving rise to jaundice are ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(76)80037-9

    authors: Badley BW

    更新日期:1976-06-01 00:00:00

  • The evaluation of autoantibodies against oxidatively modified low-density lipoprotein (LDL), susceptibility of LDL to oxidation, serum lipids and lipid hydroperoxide levels, total antioxidant status, antioxidant enzyme activities, and endothelial dysfunct

    abstract:OBJECTIVES:Behçet's disease is a multisystem disorder characterized by a chronic inflammation including acute attacks and remission periods. Decreased enzyme activity of the antioxidant system and increased levels of free radicals may have important roles in the damage of tissues observed in the disease period. In addi...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(02)00290-4

    authors: Orem A,Yandi YE,Vanizor B,Cimşit G,Uydu HA,Malkoç M

    更新日期:2002-05-01 00:00:00

  • B-type natriuretic peptide in pediatrics.

    abstract:OBJECTIVES:There has been growing interest in the use of serum B-type natriuretic peptide (BNP) and the N-terminal segment of its pro-hormone (NT-proBNP) as biomarkers for cardiac disease. The aim of this review is to summarize the current state of knowledge regarding BNP and NT-proBNP measurement in the pediatric popu...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/j.clinbiochem.2005.12.004

    authors: Davis GK,Bamforth F,Sarpal A,Dicke F,Rabi Y,Lyon ME

    更新日期:2006-06-01 00:00:00

  • Medical error, disclosure and patient safety: a global view of quality care.

    abstract::Medical errors are a prominent issue in health care. Numerous studies point at the high prevalence of adverse events, many of which are preventable. Although there is a range of severity in errors, they all cause harm, to the patient, to the system, or both. While errors have many causes, including human interactions ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/j.clinbiochem.2013.03.025

    authors: Kalra J,Kalra N,Baniak N

    更新日期:2013-09-01 00:00:00

  • Relationship between serum butyrylcholinesterase and the metabolic syndrome.

    abstract:OBJECTIVE:To identify independent predictors of serum butyrylcholinesterase (BuChE) activity and examine its relationship with the metabolic syndrome. DESIGN AND METHODS:Serum BuChE activity was measured and metabolic syndrome risk factors were assessed in 1097 healthy subjects recruited from the Newfoundland adult po...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2005.04.008

    authors: Randell EW,Mathews MS,Zhang H,Seraj JS,Sun G

    更新日期:2005-09-01 00:00:00

  • The multiple functions and mechanisms of osteopontin.

    abstract::Osteopontin (OPN) is a highly phosphorylated glycophosphoprotein having acidic characteristics and rich in aspartic acid. OPN, a multifunctional protein, has important functions on cardiovascular diseases, cancer, diabetes and kidney stone diseases and in the process of inflammation, biomineralization, cell viability ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/j.clinbiochem.2018.07.003

    authors: Icer MA,Gezmen-Karadag M

    更新日期:2018-09-01 00:00:00

  • Enzyme-linked immunosorbent assay (ELISAs) for metalloproteinase derived type II collagen neoepitope, CIIM--increased serum CIIM in subjects with severe radiographic osteoarthritis.

    abstract:OBJECTIVES:In joint degenerative diseases, the collagens are degraded by matrix metalloproteinases and protein fragments are released to serum as potential biomarkers. METHODS:A collagen type II specific neoepitope, CIIM, was identified (…RDGAAG(1053)) by mass spectrometry. Two ELISAs against the neoepitope were devel...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2011.01.001

    authors: Bay-Jensen AC,Liu Q,Byrjalsen I,Li Y,Wang J,Pedersen C,Leeming DJ,Dam EB,Zheng Q,Qvist P,Karsdal MA

    更新日期:2011-04-01 00:00:00

  • Effects of two types of medical contrast media on routine chemistry results by three automated chemistry analyzers.

    abstract:OBJECTIVES:The use of iodinated contrast media has grown in popularity in the past two decades, but relatively little attention has been paid to the possible interferential effects of contrast media on laboratory test results. Herein, we investigate medical contrast media interference with routine chemistry results obt...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2017.02.023

    authors: Park YJ,Rim JH,Yim J,Lee SG,Kim JH

    更新日期:2017-08-01 00:00:00

  • Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior.

    abstract:BACKGROUND:While investigating estrogen response element (ERE) binding properties of human estrogen receptor-α (hERα) in breast cancer cytosols, other ERE-binding proteins (ERE-BP) were observed. DESIGN AND METHODS:Recognition properties of ERE-BP were evaluated by electrophoretic mobility shift assays (EMSA) with ERE...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2013.07.005

    authors: Kruer TL,Cummins TD,Powell DW,Wittliff JL

    更新日期:2013-11-01 00:00:00

  • Determination of renal clearances of amylase/creatinine with chromogenic and enzymatic methods.

    abstract::Urinary amylase was estimated by chromogenic (amylochrome Roche) as well as enzymatic methods (SKI and Beckman: substrate starch and substrate maltotetraose respectively). Random and timed urines (24 hour collections) were analysed. Clearances of amylase gave different results dependent upon the amylase-test used and ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(79)80125-3

    authors: Hohenwallner W,Wimmer E,Sommer R

    更新日期:1979-12-01 00:00:00

  • External validation of heart-type fatty acid binding protein, high-sensitivity cardiac troponin, and electrocardiography as rule-out for acute myocardial infarction.

    abstract:OBJECTIVE:To externally validate a clinical decision rule incorporating heart fatty acid binding protein (h-FABP), high-sensitivity troponin (hs-cTn) and electrocardiogram (ECG) for the detection of acute myocardial infarction (AMI) on presentation to the Emergency Department. We also investigated whether this clinical...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2017.10.001

    authors: Van Hise CB,Greenslade JH,Parsonage W,Than M,Young J,Cullen L

    更新日期:2018-02-01 00:00:00

  • Telomerase activity, and tissue polypeptide specific antigen (TPS) in Egyptian breast cancer patients.

    abstract:OBJECTIVES:Breast cancer is the most common malignancy among Egyptian women. The aim of this study is to evaluate the role of both telomerase and TPS estimation in assessment of breast cancer. METHODS:The study included 40 patients with breast cancer, and 20 patients with benign breast diseases. Telomerase activity in...

    journal_title:Clinical biochemistry

    pub_type: 临床试验,杂志文章

    doi:10.1016/s0009-9120(01)00253-3

    authors: Kassim SK,Fawzy H,El Rassad MM,Khalil FK,Khalifa A

    更新日期:2001-09-01 00:00:00

  • Method for monitoring of the protein amino group changes during carbonylation.

    abstract:OBJECTIVES:Carbonylation of the protein amino, guanidino and thiol groups is one of the important causes of vascular complications in diabetes. We developed a simple spectrophotometric method for monitoring of the changes in the protein amino group contents during carbonylation. DESIGN AND METHODS:The method is based ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2011.05.019

    authors: Aćimović JM,Jovanović VB,Veselinović MR,Srećković VD,Mandić LM

    更新日期:2011-08-01 00:00:00

  • Quantifying MMA by SLE LC-MS/MS: Unexpected challenges in assay development.

    abstract:OBJECTIVES:Analysis of serum/plasma methylmalonic acid (MMA) is important for the diagnosis and management of methylmalonic acidemia in pediatric populations. This work focuses on developing and validating a liquid chromatography tandem mass spectrometry (LC-MS/MS) method to monitor methylmalonic acidemia using a simpl...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2016.05.010

    authors: Lo SY,Gordon C,Sadilkova K,Jack RM,Dickerson JA

    更新日期:2016-09-01 00:00:00

  • A pro-inflammatory glycoprotein biomarker is associated with lower bilirubin in metabolic syndrome.

    abstract:OBJECTIVES:Bilirubin exerts anti-oxidative and anti-inflammatory properties which may beneficially influence the development of cardio-metabolic disorders. A nuclear magnetic resonance (NMR) spectroscopy-based glycoprotein biomarker, designated GlycA, whose signal originates from several glycosylated acute-phase protei...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2015.06.016

    authors: Dullaart RP,Gruppen EG,Connelly MA,Lefrandt JD

    更新日期:2015-11-01 00:00:00

  • Inhibition of serum protein binding of thyroxine in a hypothyroid patient with familial dysalbuminemic hyperthyroxinemia.

    abstract:OBJECTIVE:To investigate unusual free thyroxine (FT4) responses to T4 replacement doses in a hypothyroid patient with familial dysalbuminemic hyperthyroxinemia (FDH). METHODS:In this FDH hypothyroid patient, serum FT4 concentration by equilibrium dialysis and T4, triiodothyronine (T3), and thyroid stimulating hormone ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/0009-9120(95)02016-0

    authors: Premachandra BN,Wortsman J,Williams IK

    更新日期:1996-02-01 00:00:00

  • Solid phase measurements of antibody and lectin binding to xenogenic carbohydrate antigens.

    abstract:OBJECTIVES:In future pig-to-man xenotransplantation it is important to master tools that identify potentially xenogenic alphagalactose (Galalpha) antigens in the doner tissue. DESIGN AND METHODS:We have measured the binding potentials of Galalpha detecting lectins and antibodies, including a naturally occurring subfra...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(03)00120-6

    authors: Kirkeby S,André S,Gabius HJ

    更新日期:2004-01-01 00:00:00

  • Changes in serum obestatin, preptin and ghrelins in patients with Gestational Diabetes Mellitus.

    abstract:OBJECTIVES:The present study aims to establish the levels of acylated ghrelin, desacylated ghrelin, obestatin and preptin, during pregnancy and the postpartum period in pregnant women with Gestational Diabetes Mellitus (GDM) and healthy pregnancy women. DESIGN AND METHODS:The study registered 20 pregnant women with GD...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2011.08.1131

    authors: Baykus Y,Gurates B,Aydin S,Celik H,Kavak B,Aksoy A,Sahin I,Deniz R,Gungor S,Guzel SP,Minareci Y

    更新日期:2012-02-01 00:00:00

  • Plasma arginase concentration measured by an enzyme-linked immunosorbent assay (ELISA) in normal adult population.

    abstract::Human liver arginase has many biological effects on lymphocytes, macrophages, liver cells, and tumor cells, in addition to its major role in the liver urea cycle. We have developed a sandwich enzyme-linked immunosorbent assay (ELISA) method to quantitate arginase concentrations in plasma that can be applied to various...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/0009-9120(93)80009-j

    authors: Wang SR,Chen ML,Huang MH,Lin HY,Tsai JJ,Kuo BI

    更新日期:1993-12-01 00:00:00

  • Screening for cancer in high risk families.

    abstract:OBJECTIVE:To review screening for cancer in high risk families. METHODS AND RESULTS:Screening for hereditary cancer involves three steps: it is first necessary to identify families at high risk by examining the number and sites of cancer in a family. Special attention is given to cancers appearing at an early age, to ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/0009-9120(95)00022-2

    authors: Narod SA

    更新日期:1995-08-01 00:00:00

  • ISO 15189 accreditation: Requirements for quality and competence of medical laboratories, experience of a laboratory I.

    abstract:AIM:Medical laboratories are the key partners in patient safety. Laboratory results influence 70% of medical diagnoses. Quality of laboratory service is the major factor which directly affects the quality of health care. The clinical laboratory as a whole has to provide the best patient care promoting excellence. METH...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2008.09.011

    authors: Guzel O,Guner EI

    更新日期:2009-03-01 00:00:00

  • Association of serum bilirubin levels with albuminuria in patients with essential hypertension.

    abstract:OBJECTIVES:This study was undertaken to evaluate the relationship between serum bilirubin concentrations and the degree of urinary albumin excretion in hypertensive patients. DESIGN AND METHODS:A total of 120 hypertensive subjects were enrolled, in which 80 (67%) with normoalbuminuria (albumin excretion rate [AER] of ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2011.03.137

    authors: Huang SS,Huang PH,Chiang KH,Chen JW,Lin SJ

    更新日期:2011-07-01 00:00:00

  • Correlation between the administered dose of DHEA and serum levels of DHEA and DHEA-S in human volunteers: analysis of published data.

    abstract:OBJECTIVES:Studies from both experimental animals and humans suggest that administration of exogenous DHEA may have beneficial endocrine-metabolic, immunologic and neurologic effects. Several groups have administered DHEA to humans, but to the best of our knowledge, no one at this point has published a summary of the r...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(99)00021-1

    authors: Tummala S,Svec F

    更新日期:1999-07-01 00:00:00

  • A simplified assay for serum 25-hydroxycalciferol.

    abstract::The measurement of serum 25-hydroxycalciferol (25-OH D) has proved to be a reliable index of the vitamin D nutritional status in man providing the renal 25-hydroxycalciferol-1 alpha-hydroxylase is functional. Since it is known that 25 OHD binds to alpha-globulins for its transport to the kidney, we have developed a co...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(80)90735-3

    authors: Delvin EE,Dussault M,Glorieux FH

    更新日期:1980-06-01 00:00:00

  • The TCN2 776CNG polymorphism correlates with vitamin B(12) cellular delivery in healthy adult populations.

    abstract:OBJECTIVES:Vitamin B(12), or B(12), is an essential nutrient for humans, and its deficiency is a public health problem, especially in elderly population. Around 30% of circulating total B(12) levels are attached to transcobalamin II (TCN2), being referred as holotranscobalamin (holo-TC), and representing the biological...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2010.01.015

    authors: Castro R,Barroso M,Rocha M,Esse R,Ramos R,Ravasco P,Rivera I,de Almeida IT

    更新日期:2010-05-01 00:00:00

  • Plasma concentrations of Gas6 and soluble Axl correlate with disease and predict mortality in patients with critical limb ischemia.

    abstract:INTRODUCTION:Critical limb ischemia (CLI) is a severe peripheral arterial disease, characterized by rest pain, ulcers and gangrene in the legs. Gas6 is a vitamin K-dependent protein, which binds and activates the tyrosine kinase receptor Axl. Gas6-mediated Axl-signaling influences endothelial activation, neointima form...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2010.04.006

    authors: Ekman C,Gottsäter A,Lindblad B,Dahlbäck B

    更新日期:2010-07-01 00:00:00

  • Susceptibility of erythrocyte lipids to oxidation and erythrocyte antioxidant status in myocardial infarction.

    abstract:OBJECTIVE:We evaluated the erythrocyte lipid susceptibility to oxidation and erythrocyte antioxidant status in patients with myocardial infarction (MI). DESIGN AND METHODS:Patients with MI were divided into two group according to the severity of the disease as severe (n = 30) or milder (n = 25). Malondialdehyde as a m...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(98)00070-8

    authors: Konukoğlu D,Akçay T,Erdem T

    更新日期:1998-11-01 00:00:00