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Urine flow cytometer quantification of leukocytes in samples containing a large proportion of lymphocytes.

Abstract：

OBJECTIVE:We compared leukocyte counts obtained by cytometric analysis and Fuchs-Rosenthal (FR) chamber counting in different proportions of lymphocytes (Lym%) suspensions and cerebrospinal fluid (CSF). DESIGN AND METHODS:UF-100 (UF) was evaluated. For preparation of cell suspensions, gradient density centrifugation method was used. RESULTS:The regression equation for UF and FR chamber counting of the cell suspensions was y=0.88x+18.8 WBC/microL (r=0.832, n=106). For a few high Lym% samples, markedly underestimated WBC counts were obtained by UF. CONCLUSIONS:Underestimated WBC count is due not to systematic error but to random error. Counts of the "other" population by UF may be useful for detection of underestimated samples.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Yamanishi H,Imai N,Ohmine T,Nishiyama M,Suehisa E,Kanakura Y,Iwatani Y

doi

10.1016/j.clinbiochem.2006.05.003

subject

Has Abstract

pub_date

2006-08-01 00:00:00

pages

857-9

issue

8

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(06)00157-3

journal_volume

39

pub_type

杂志文章

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