Preanalytic of total antioxidant capacity assays performed in serum, plasma, urine and saliva.

Abstract:

:The investigation of oxidative stress (OS), its mechanisms and connections with human diseases, is a topic of interest with more than 36,000 PubMed citations to date. The OS can be approached either from the perspective of pro-oxidation, either of anti-oxidation, and both can be investigated considering individual chemical constituents or their pooled effect. Actually, as it is for any laboratory assay, whatever source of variability introduces a bias potentially undermining the test results regardless of its application. In this regard, the effect of sample collection, handling and storage - that collectively constitute the preanalytical phase - on the likeliness of the measured value represent a major challenge for any researcher. In this review, we will deal with methods devised to assess the so-called total antioxidant capacity (TAC), which represents the sinking capability expressed toward a given load of pro-oxidant species. Thus, it will be presented the information available to date on the preanalytical phase of TAS assessment, focusing on the issues that strictly concern the preservation of antioxidants within the specimen. Obviously, preanalytic should represent a first concern in any laboratory research, to which this work could contribute giving practical indications and raising the researchers' awareness about the issue.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Ialongo C

doi

10.1016/j.clinbiochem.2016.11.037

subject

Has Abstract

pub_date

2017-04-01 00:00:00

pages

356-363

issue

6

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(16)30422-2

journal_volume

50

pub_type

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