Abstract:
OBJECTIVES:Accurate measurement of IgG subclass (IgGSc) levels are essential to aid in the diagnosis of disease states such as primary immunodeficiencies. However, there is no single standardisation of nephelometric and turbidimetric assays for these analytes and two reference materials have been utilised. We expand on previous reports and present data from a multi-site analysis that both identifies and quantitatively defines the differences in calibration resulting from the use of different reference materials. DESIGN AND METHODS:IgGSc antibodies in the serum specimens and reference materials were measured according to the manufacturers' instructions using commercially available IgGSc assays or components. RESULTS:Data from four independent sites showed that in spite of the different commercial suppliers of IgGSc assays calibrating to different reference materials, ERM-DA470k and WHO67 /97, the resulting calibrations were comparable for IgG1 and IgG2. However, for IgG3 and IgG4 the calibrations were significantly different. The use of assay specific normal ranges should compensate for these calibration differences, however, the two manufacturers' assays can give differing clinical classifications. The agreement between the different manufacturers' IgGSc assays was between 85.1% and 95.8% for all IgGSc assays, the discordance of sample classification for IgG1 and IgG2 assays was approximately 12% and 15% respectively, whilst that for IgG3 and IgG4 was 4% and 13% respectively. CONCLUSION:We discuss the similarities and differences between assays that utilise the different reference materials.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Wilson C,Ebling R,Henig C,Adler T,Nicolaevski R,Barak M,Cazabon J,Maisin D,Lepoutre T,Gruson D,Hughes RG,Parker ARdoi
10.1016/j.clinbiochem.2013.07.014subject
Has Abstractpub_date
2013-11-01 00:00:00pages
1751-5issue
16-17eissn
0009-9120issn
1873-2933pii
S0009-9120(13)00347-0journal_volume
46pub_type
杂志文章abstract::Macro creatine kinase type 2 (MCK-2) is a promising new tumour marker for carcinoma of the colon. We have purified it from tumour tissue, from serum and, for the first time, from normal colonic epithelium and identified it in a tissue culture line of colon cancer cells. The molecular weight of each preparation, regard...
journal_title:Clinical biochemistry
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journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
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abstract:OBJECTIVES:This study examines the redox status of drug-resistant epileptic patients and how it is modified after surgical treatment. DESIGN AND METHODS:The activity of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase), and markers of damage to biomolecules (malondialdehyde and advanced o...
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abstract:OBJECTIVES:Cortisol metabolism is controlled by 11beta-hydroxysteroid dehydrogenase (11beta-HSD) isoenzymes, which interconvert cortisol and cortisone. Accurate measurement of the cortisol and cortisone concentrations and their ratio provide useful information about 11beta-HSD activity. METHODS:Cortisol and cortisone ...
journal_title:Clinical biochemistry
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abstract::Mycophenolate mofetil is widely used in combination with either cyclosporine or tacrolimus for rejection prophylaxis in renal and heart transplant patients. Although not monitored routinely nearly to the degree that other agents such as cyclosporine or tacrolimus, there is an expanding body of experimental evidence fo...
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doi:10.1016/s0009-9120(00)00184-3
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journal_title:Clinical biochemistry
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doi:10.1016/j.clinbiochem.2012.04.017
更新日期:2012-10-01 00:00:00
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更新日期:1994-12-01 00:00:00
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doi:10.1016/j.clinbiochem.2005.11.008
更新日期:2006-02-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/0009-9120(92)80047-k
更新日期:1992-04-01 00:00:00
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journal_title:Clinical biochemistry
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doi:10.1016/j.clinbiochem.2010.08.008
更新日期:2011-01-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2010.07.003
更新日期:2010-11-01 00:00:00
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更新日期:2018-03-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2010.07.007
更新日期:2010-09-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/0009-9120(87)90011-7
更新日期:1987-12-01 00:00:00
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doi:10.1016/s0009-9120(96)00159-2
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doi:10.1016/j.clinbiochem.2010.08.015
更新日期:2010-11-01 00:00:00
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doi:10.1016/j.clinbiochem.2004.05.015
更新日期:2004-07-01 00:00:00
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更新日期:2018-06-01 00:00:00
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journal_title:Clinical biochemistry
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doi:10.1016/j.clinbiochem.2015.07.019
更新日期:2016-02-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2009.06.007
更新日期:2009-09-01 00:00:00
abstract::Lipoprotein lipase (LPL) of adipose cells is present only in membrane compartments, mainly in the Golgi apparatus. LPL is a typical secretory protein which appears to be active as a homodimer. The process of LPL synthesis and maturation requires multiple steps. LPL is synthesized in the endoplasmic reticulum as an ina...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/0009-9120(90)90034-r
更新日期:1990-10-01 00:00:00
abstract::Core Alzheimer's Disease (AD) biomarkers are the cerebrospinal fluid (CSF) proteins amyloid β42 and β40, and the tau proteins, total and phosphorylated. Their use is recommended by research guidelines for diagnostic purpose and for stratification of patients for clinical trials. However, novel potential biomarkers are...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2019.08.008
更新日期:2019-10-01 00:00:00
abstract::A highly specific enzyme-linked "sandwich" immunoassay was developed for determining cardiac myosin light chain II (MLC II) in serum by using an anticardiac MLC II monoclonal antibody and a solid phase consisting of glass rods coated with another monoclonal antibody. We can detect as little as 0.2 ng of cardiac MLC II...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/0009-9120(90)80042-h
更新日期:1990-12-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2007.03.005
更新日期:2007-06-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2005.01.012
更新日期:2005-06-01 00:00:00
abstract:OBJECTIVES:To compare the accuracy of procalcitonin (PCT) in early-onset neonatal sepsis (EOS) using standard cut-off values and a multilevel probabilistic approach. DESIGN AND METHODS:A retrospective study of PCT was performed in 149 newborns at risk of EOS, including preterm or prolonged rupture of membranes, chorio...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2008.05.015
更新日期:2008-10-01 00:00:00
abstract:BACKGROUND:The p53 gene is an established tumor suppressor and an inducer of apoptosis. We here attempt to determine whether the putative anticarcinogenic properties attributed to red wine and its polyphenolic constituents depend, at least in part, upon their ability to modulate p53 expression in cancer cells. METHODS...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(01)00246-6
更新日期:2001-07-01 00:00:00