当前位置： SCI文献检索 > CLINICAL BIOCHEMISTRY期刊下所有文献 > Simultaneous determination of fluoxetine and norfluoxetine in dried blood spots using high-performance liquid chromatography-tandem mass spectrometry.

Simultaneous determination of fluoxetine and norfluoxetine in dried blood spots using high-performance liquid chromatography-tandem mass spectrometry.

Abstract：

BACKGROUND:Therapeutic drug monitoring (TDM) of the widely prescribed antidepressant fluoxetine (FLU) is recommended in certain situations, such as occurrence of toxicity, inadequate response or suspect of poor adherence. Dried blood spot (DBS) sampling is an increasingly studied alternative for TDM, particularly for outpatients, due to its ease of collection and inherent stability. OBJECTIVES:The aim of this study was to develop and validate an LC-MS/MS assay for the simultaneous quantification of FLU and norfluoxetine (NFLU) in DBS. DESIGN AND METHODS:The assay is based on a liquid extraction of single DBS with 8mm of diameter, using FLU-D6 as the internal standard, followed by reversed phase separation in an Accucore® C18 column (100×2.1mm, 2.6μm). Mobile phase was composed of water and acetonitrile (gradient from 80:20 to 50:50, v/v), both containing formic acid 0.1%. The assay was validated and applied to 30 patients under FLU pharmacotherapy. RESULTS:The assay was linear in the range 10-750ngmL-1. Precision assays presented CV% of 3.13-9.61 and 3.54-7.99 for FLU and NFLU, respectively, and accuracy in the range of 97.98-110.44% and 100.25-105.8%. FLU and NFLU were stable at 25 and 45°C for 7days. The assay was evaluated in 30 patients under FLU treatment. Concentrations of both compounds were higher in DBS than in plasma, and the use of the multiplying factors 0.71 and 0.68 for FLU and NFLU, respectively, allowed acceptable estimation of plasma concentrations, with median prediction bias of -0.55 to 0.55% and mean differences of 0.4 to 2.2ngmL-1. CONCLUSIONS:The presented data support the clinical use of DBS for therapeutic drug monitoring of FLU.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

da Silva ACC,Raasch JR,Vargas TG,Peteffi GP,Hahn RZ,Antunes MV,Perassolo MS,Linden R

doi

10.1016/j.clinbiochem.2017.10.002

subject

Has Abstract

pub_date

2018-02-01 00:00:00

pages

85-93

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(17)30794-4

journal_volume

52

pub_type

杂志文章

相关文献

CLINICAL BIOCHEMISTRY文献大全
  • The effect of specimen collection additives and sample storage on the Dupont ACA glucose method.

    abstract：:Two studies are described in this paper for the purpose of extending the application of the Dupont ACA glucose method to (1) analysis of specimens containing sodium fluoride and sodium iodoacetate, and (2) analysis of specimens containing EDTA, provided analysis is conducted immediately following specimen collection a...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(82)96520-1

    authors： Dubois JA,Roberts G,Beekman J

    更新日期：1982-04-01 00:00:00

  • Performance characteristics of six automated 25-hydroxyvitamin D assays: Mind your 3s and 2s.

    abstract：BACKGROUND:Measurement of 25(OH)D has evolved rapidly, with an increased number of high-throughput automated immunoassays becoming available in recent years. The aim of this study was to fully evaluate six commercially available automated 25(OH)D immunoassays, including 2 newly available (Beckman Coulter) and one recal...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2015.08.005

    authors： Wyness SP,Straseski JA

    更新日期：2015-11-01 00:00:00

  • Relationship between measured average glucose by continuous glucose monitor and HbA1c measured by three different routine laboratory methods.

    abstract：OBJECTIVES:The relationship between glycated hemoglobin A1c (HbA1c) and average glucose has been described by the empirically derived estimated average glucose (eAG) equation in the A1c-Derived Average Glucose (ADAG) study, with extensive calibration efforts in four secondary reference HbA1c methods. It is not known if...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2015.02.012

    authors： Loh TP,Sethi SK,Wong MS,Tai ES,Kao SL

    更新日期：2015-05-01 00:00:00

  • Low basal levels of circulating adiponectin in patients undergoing coronary stenting predict in-stent restenosis, independently of basal levels of inflammatory markers: lipoprotein associated phospholipase A2, and myeloperoxidase.

    abstract：OBJECTIVE:The aim of this study was to find a pre-interventional marker with the capacity to predict in-stent restenosis (ISR). Considering the anti-atherosclerotic role of adiponectin (APO), an adipocytokine with anti-inflammatory, anti-proliferative, anti-oxidative and anti-thrombotic properties, low plasma levels of...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2008.09.109

    authors： Moldoveanu E,Mut-Vitcu B,Tanaseanu GR,Marta DS,Manea G,Kosaka T,Vidulescu C,Tanaseanu C

    更新日期：2008-12-01 00:00:00

  • Application of denaturing gradient gel electrophoresis (DGGE) to screen for mutations of the human glucocorticoid receptor alpha gene (hGRalpha).

    abstract：OBJECTIVES:In a previous publication, we had presented a sensitive method to detect mutations of the segment of the human glucocorticoid receptor alpha (hGRalpha) gene encoding the ligand binding domain (LBD) and part of the DNA binding domain (DBD) of hGRalpha, as several types of glucocorticoid resistance syndromes h...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(03)00027-4

    authors： Tsolakidou AF,Coulocheri SA,Sekeris CE,Moutsatsou P

    更新日期：2003-06-01 00:00:00

  • Multicenter assessment of a hemoglobin A1c point-of-care device for diagnosis of diabetes mellitus.

    abstract：OBJECTIVE:A multisite investigation compared the analytical performance of a point-of-care (POC) HbA1c device with multiple commonly used HbA1c laboratory methods and an NGSP (National Glycohemoglobin Standardization Program) reference method. RESEARCH DESIGN AND METHODS:The Afinion AS100 POC device analyzed HbA1c usi...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.clinbiochem.2018.09.007

    authors： Sobolesky PM,Smith BE,Saenger AK,Schulz K,Apple FS,Scott MG,Wu AHB,Little RR,Fitzgerald RL

    更新日期：2018-11-01 00:00:00

  • Recent developments in meconium and hair testing methods for the confirmation of gestational exposures to alcohol and tobacco smoke.

    abstract：:The use of alcohol and tobacco is prevalent among pregnant women despite the well-known adverse effects of these substances imposed on the developing fetus and immense public health education efforts. Confirmation of gestational exposures to these compounds have relied mostly on maternal self-reporting, which is often...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.clinbiochem.2004.01.010

    authors： Chan D,Caprara D,Blanchette P,Klein J,Koren G

    更新日期：2004-06-01 00:00:00

  • The role of interleukin-12 in the pathogenesis of psoriasis.

    abstract：OBJECTIVES:To verify the role of IL-12 in the pathogenesis of psoriasis and determine its relation to IFNgamma. DESIGN AND METHODS:Skin biopsies from lesional and non-lesional skin of 30 patients and 10 healthy controls were obtained for quantitative PCR examination of IL-12 (P40) and IFNgamma mRNA as well as in situ ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2005.11.008

    authors： Shaker OG,Moustafa W,Essmat S,Abdel-Halim M,El-Komy M

    更新日期：2006-02-01 00:00:00

  • CRISPR-based tools: Alternative methods for the diagnosis of COVID-19.

    abstract：:The recently emerged severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) spread all over the world rapidly and caused a global pandemic. To prevent the virus from spreading to more individuals, it is of great importance to identify and isolate infected individuals through testing. Reverse transcription-quanti...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.clinbiochem.2020.12.011

    authors： Palaz F,Kalkan AK,Tozluyurt A,Ozsoz M

    更新日期：2021-01-09 00:00:00

  • Performance characteristics of the Access AMH assay for the quantitative determination of anti-Müllerian hormone (AMH) levels on the Access* family of automated immunoassay systems.

    abstract：OBJECTIVES:Anti-Müllerian hormone (AMH) measurement is useful as an aid in the evaluation of ovarian reserve. In the past, its conventional use was restricted by the low-throughput and variability of existing manual AMH assays. We developed the automated Access AMH assay for the quantitative determination of AMH levels...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2016.08.005

    authors： Demirdjian G,Bord S,Lejeune C,Masica R,Rivière D,Nicouleau L,Denizot P,Marquet PY

    更新日期：2016-11-01 00:00:00

  • A simple matrix of analytical performance to identify assays that risk patients using External Quality Assurance Program data.

    abstract：OBJECTIVES:We propose a simple way to reliably rank assays for improvement according to patient risk, based solely on EQA imprecision and biological variation data. Because the underlying technique aligns the imprecision class of an assay from EQA data, peer performance can be used to assess achievable imprecision and ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2016.01.014

    authors： Mackay M,Hegedus G,Badrick T

    更新日期：2016-05-01 00:00:00

  • A comparative study of three approaches to the routine quantitation of human serum proteins.

    abstract：:The Laser Nephelometer PDQTM (Hyland Division, Travenol Laboratories Inc.) and the Abbott Bichromatic Analyser 100 (Abbott Laboratories) were compared with a radial immunodiffusion method. Seventy-eight serum specimens collected during routine blood testing were aliquoted and quantitated by the three procedures descri...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(78)90126-1

    authors： Bruver RM,Salkie ML

    更新日期：1978-06-01 00:00:00

  • The differential diagnostic model for serous peptidomics in HBV carriers established by MALDI-TOF-MS analysis.

    abstract：OBJECTIVES:Hepatitis B virus (HBV) can result in asymptomatic carrier (AsC) state or chronic inflammation of liver, which depends on the host immunity. We therefore investigated the peptidomic profiling in the process of HBV infection. DESIGN AND METHODS:In this study, serum from 116 HBV infected (AsC and chronic hepa...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2013.10.016

    authors： Tian L,Wang Y,Xu D,Gao Y,Wen X,Tian Y

    更新日期：2014-01-01 00:00:00

  • Comparison of umbilical cord tissue and meconium for the confirmation of in utero drug exposure.

    abstract：OBJECTIVES:Drug screening in neonates is traditionally performed using meconium, but cord tissue has been proposed as an alternative specimen. This study compares the detection of drugs in a large number of paired meconium and umbilical cord tissue samples from subjects at risk of in utero drug exposure. DESIGN AND ME...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2017.03.006

    authors： Colby JM

    更新日期：2017-09-01 00:00:00

  • Significant, quantifiable differences exist between IgG subclass standards WHO67/97 and ERM-DA470k and can result in different interpretation of results.

    abstract：OBJECTIVES:Accurate measurement of IgG subclass (IgGSc) levels are essential to aid in the diagnosis of disease states such as primary immunodeficiencies. However, there is no single standardisation of nephelometric and turbidimetric assays for these analytes and two reference materials have been utilised. We expand on...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2013.07.014

    authors： Wilson C,Ebling R,Henig C,Adler T,Nicolaevski R,Barak M,Cazabon J,Maisin D,Lepoutre T,Gruson D,Hughes RG,Parker AR

    更新日期：2013-11-01 00:00:00

  • Soluble serum Klotho in diabetic nephropathy: relationship to VEGF-A.

    abstract：OBJECTIVES:Both kidney expression and soluble serum Klotho are influenced by chronic kidney disease (CKD) and diabetes. Serum Klotho is a yet poorly explored biomarker. We describe, for the first time to our knowledge, serum Klotho in diabetic patients with CKD and its relationship to vascular endothelial growth factor...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2012.07.098

    authors： Kacso IM,Bondor CI,Kacso G

    更新日期：2012-11-01 00:00:00

  • The first study of galactose-1-phosphate uridyl transferase mutations in Iranian galactosemia patients.

    abstract：OBJECTIVES:Classical galactosemia (McKusick 230400) is an autosomal recessive disorder caused by mutations in the galactose-1-phosphate uridyl transferase (GALT;EC 2.7.7.10) gene. DESIGN AND METHODS:In the present study, we report molecular analysis of 14 unrelated Iranian galactosemia children with reduced or without...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2006.04.020

    authors： Mirzajani F,Mirfakhraie R,Nabati F,Tabatabaei NN,Talachian E,Houshmand M

    更新日期：2006-07-01 00:00:00

  • Association of chitotriosidase enzyme activity and genotype with the risk of nephropathy in type 2 diabetes.

    abstract：OBJECTIVE:The immune-inflammatory system has been implicated in the pathogenesis of diabetic nephropathy; however, many of the mechanisms involved remain unclear. Chitotriosidase enzyme is an active human chitinase and a major protein product of activated macrophages. Although playing an important role in innate and ac...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2015.11.009

    authors： Elmonem MA,Amin HS,El-Essawy RA,Mehaney DA,Nabil M,Kamel LN,Farid IM

    更新日期：2016-04-01 00:00:00

  • Serum cystatin C as a better marker of vancomycin clearance than serum creatinine in elderly patients.

    abstract：OBJECTIVES:The purpose of this study is to assess the usefulness of the concentration of cystatin C (Cys-C) in serum for predicting the clearance of vancomycin (CLvcm) compared with the serum concentration of creatinine (SCr) in the elderly. METHODS:Thirty-nine serum samples were obtained from 24 elderly patients (65 ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2007.01.008

    authors： Okamoto G,Sakamoto T,Kimura M,Ukishima Y,Sonoda A,Mori N,Kato Y,Maeda T,Kagawa Y

    更新日期：2007-04-01 00:00:00

  • Stability of analytes related to clinical chemistry and bone metabolism in blood specimens after delayed processing.

    abstract：OBJECTIVES:We investigated the stability of 36 analytes related to clinical chemistry in a controlled storage study. DESIGN AND METHODS:Blood was collected from 11 subjects and was maintained for 45 min, 2.5 h, 5 h, or 24 h after phlebotomy before centrifugation. RESULTS:Statistically significant changes were observe...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2009.02.010

    authors： Zwart SR,Wolf M,Rogers A,Rodgers S,Gillman PL,Hitchcox K,Ericson KL,Smith SM

    更新日期：2009-06-01 00:00:00

  • Histamine quantification in human plasma using high resolution accurate mass LC-MS technology.

    abstract：BACKGROUND:Histamine (HA) is a small amine playing an important role in anaphylactic reactions. In order to identify and quantify HA in plasma matrix, different methods have been developed but present several disadvantages. Here, we developed an alternative method using liquid chromatography coupled with an ultra-high ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2015.08.012

    authors： Laurichesse M,Gicquel T,Moreau C,Tribut O,Tarte K,Morel I,Bendavid C,Amé-Thomas P

    更新日期：2016-01-01 00:00:00

  • Development of an immunoassay for differentiating human immunodeficiency virus infections--from vaccine-induced immune response in Tiantan vaccine trials in China.

    abstract：OBJECTIVE:To develop a Rapid Flow-through assay for distinguishing replicating Tiantan vaccine-generated serological response from true HIV infection. DESIGN AND METHOD:A Rapid Flow-through Test including gp41, gp36, sk1, sk2 and sk3 antigens was established and the performance of the assay was evaluated in clinical s...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2012.05.013

    authors： Yuan Z,Chen W,Zhang J,Zhang J,Xiang T,Hu J,Wu Z,Du X,Huang A,Zheng J

    更新日期：2012-10-01 00:00:00

  • Genetic determination of plasma lipids and insulin in the Czech population.

    abstract：OBJECTIVES:To evaluate the association between plasma lipids and insulin and variation in the genes for apolipoproteins (APO) E (CfoI), B (insertion/deletion), C1 (HpaI), and C3 (C-482T, C3238G) in a population-based Czech Slavonic study. DESIGN AND METHODS:In 131 men and 154 women, polymorphisms were investigated usi...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(01)00184-9

    authors： Hubacek JA,Waterworth DM,Poledne R,Pitha J,Skodová Z,Humphries SE,Talmud PJ

    更新日期：2001-03-01 00:00:00

  • Use of different chemical methods for acid phosphatase in cases of rape.

    abstract：:Generally acid phosphatase (ACP) assay is used for testing cases of alleged rape. Comparison of three different chemical methods for Acid Phosphatase (Andersch's method with p-nitrophenyl-phosphate substrate and tartrate inhibitor (A-Tart), Roy's method with thymolphthalein phosphate substrate (R-TMP), and Babson's me...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(77)92784-9

    authors： Pragay DA,Casey SJ,Gotthelf J

    更新日期：1977-10-01 00:00:00

  • A pro-inflammatory glycoprotein biomarker is associated with lower bilirubin in metabolic syndrome.

    abstract：OBJECTIVES:Bilirubin exerts anti-oxidative and anti-inflammatory properties which may beneficially influence the development of cardio-metabolic disorders. A nuclear magnetic resonance (NMR) spectroscopy-based glycoprotein biomarker, designated GlycA, whose signal originates from several glycosylated acute-phase protei...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2015.06.016

    authors： Dullaart RP,Gruppen EG,Connelly MA,Lefrandt JD

    更新日期：2015-11-01 00:00:00

  • Inhibition of serum protein binding of thyroxine in a hypothyroid patient with familial dysalbuminemic hyperthyroxinemia.

    abstract：OBJECTIVE:To investigate unusual free thyroxine (FT4) responses to T4 replacement doses in a hypothyroid patient with familial dysalbuminemic hyperthyroxinemia (FDH). METHODS:In this FDH hypothyroid patient, serum FT4 concentration by equilibrium dialysis and T4, triiodothyronine (T3), and thyroid stimulating hormone ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/0009-9120(95)02016-0

    authors： Premachandra BN,Wortsman J,Williams IK

    更新日期：1996-02-01 00:00:00

  • An overview of the kallikrein gene families in humans and other species: emerging candidate tumour markers.

    abstract：:Kallikreins are serine proteases with diverse physiologic functions. They are represented by multigene families in many animal species, especially in rat and mouse. Recently, the human kallikrein gene family has been fully characterized and includes 15 members, tandemly localized on chromosome 19q13.4. A new definitio...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/s0009-9120(03)00055-9

    authors： Yousef GM,Diamandis EP

    更新日期：2003-09-01 00:00:00

  • Transthyretin as a marker to predict outcome in critically ill patients.

    abstract：BACKGROUND:A determination of serum Transthyretin (TTR, Prealbumin) level is an objective method of assessing protein catabolic loss of severely ill patients and numerous studies have shown that TTR levels correlate with patient outcomes of non-critically ill patients. We evaluated whether TTR level correlates with the...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2008.06.016

    authors： Devakonda A,George L,Raoof S,Esan A,Saleh A,Bernstein LH

    更新日期：2008-10-01 00:00:00

  • Precipitation method for separating and quantifying bone and liver alkaline phosphatase isoenzymes.

    abstract：:We evaluated a method for quantifying bone isoenzyme of alkaline phosphatase (ALP) which utilizes wheat-germ lectin to precipitate this fraction. In precision studies, CVs ranged from 3.2 to 11.4% (within-day) and from 3.7 to 11.5% (day-to-day). The assay procedure was linear to 1100 U/L and was easily adapted to auto...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(05)80017-7

    authors： Burlina A,Plebani M,Secchiero S,Zaninotto M,Sciacovelli L

    更新日期：1991-10-01 00:00:00

  • Serum retinol binding protein 4 in patients with familial partial lipodystrophy.

    abstract：OBJECTIVE:To determine Retinol Binding Protein 4 (RBP4) levels in patients with Familial Partial Lipodystrophy (FPLD). METHODS:Ten patients with FPLD and a control group (9 patients) were selected to participate in the study. RESULTS:RBP4-log levels were lower in patients with FPLD in comparison to control group (1.5...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2009.03.006

    authors： Godoy-Matos AF,Moreira RO,MacDowell R,Bendet I,Mory PB,Moises RS

    更新日期：2009-07-01 00:00:00