Abstract:
BACKGROUND:Oxidative stress is evident from an early stage in chronic kidney disease (CKD). Therefore, we investigated redox biomarkers in polycystic kidney disease (ADPKD) and IgA nephropathy (IGAN). METHODS:This is a case-control study with three groups: ADPKD (n = 54), IGAN (n = 58) and healthy controls (n = 86). The major plasma aminothiols with their redox species were examined: homocysteine (Hcy), cysteinglycine (CG), cysteine (Cys) and glutathione (GSH). The redox ratio was the ratio of reduced free and oxidized aminothiols in plasma. We investigated malonedialdehyde (MDA) and advanced oxidation protein products (AOPP), and ten single nucleotide polymorphisms of antioxidant enzymes. RESULTS:Patients had elevated oxidized free Hcy and Cys with associated low redox ratios - most pronounced in IGAN. Patients with IGAN had elevated AOPP and possibly MDA. Oxidized free Hcy and Cys with redox ratios were correlated to AOPP, MDA and proteinuria. Furthermore, there was an independent relationship to parathyroid hormone (PTH). IGAN had an elevated frequency of Val16Ala SNP rs4880, which influence the function of mitochondrial superoxide dismutase 2 (p = 0.03). CONCLUSIONS:Patients with ADPKD and IGAN have evidence of oxidative stress from stage 1 to 4 - most pronounced in IGAN. In patients, aminothiol redox biomarkers were correlated to AOPP, proteinuria and PTH, which are known prognostic markers in CKD. It may be possible that oxidative stress influences PTH dysregulation in CKD. The association between IGAN and the redox related variant allele rs4880(C) might indicate a new susceptibility locus for IGAN, but this needs verification.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Tariq A,Mansoor MA,Marti HP,Jonsson G,Slettan A,Weeraman P,Apeland Tdoi
10.1016/j.clinbiochem.2018.04.010subject
Has Abstractpub_date
2018-06-01 00:00:00pages
33-40eissn
0009-9120issn
1873-2933pii
S0009-9120(17)31049-4journal_volume
56pub_type
杂志文章abstract::During the past decade, tandem mass spectrometry hyphenated to liquid chromatography separation systems (HPLC-MS/MS) has developed to an important technology in clinical chemistry - not only for research purposes but also for routine use. At present, most important application fields are target analyses in therapeutic...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2008.02.017
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVE:Recently cortisol level in hair is supposed to be a good biomarker of chronic stress. We had developed a new method based on HPLC-FLU for identification of cortisol in human hair and measured cortisol distribution of each 1-cm segment along hair shaft. DESIGN AND METHODS:Hair sample was pulverized and incuba...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2010.01.014
更新日期:2010-05-01 00:00:00
abstract::Proteomics, the analysis of expressed proteins, has been an important developing area of research for the past two decades [Anderson, NG, Anderson, NL. Twenty years of two-dimensional electrophoresis: past, present and future. Electrophoresis 1996;17:443-453]. Advances in technology have led to a rapid increase in app...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2004.09.002
更新日期:2004-11-01 00:00:00
abstract::Although a multitude of hepatitis B markers are available to discriminate different states of hepatitis B virus (HBV) infection, there remains a need to detect the HBV DNA itself. The HBV DNA test is the only test that can quantify the number of complete virions. As shown in this review, the diagnostic value of testin...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/0009-9120(93)90127-r
更新日期:1993-08-01 00:00:00
abstract::We compared results of urine drug analysis with clinical data and history to test the usefulness of peripartum drug screening and to establish guidelines for optimal testing. Urine from 28 mothers and 52 babies was analysed. Drugs not suspected by history were found in 10 mothers and six babies. Results assisted in th...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(88)80113-9
更新日期:1988-01-01 00:00:00
abstract::Osteopontin (OPN) is a highly phosphorylated glycophosphoprotein having acidic characteristics and rich in aspartic acid. OPN, a multifunctional protein, has important functions on cardiovascular diseases, cancer, diabetes and kidney stone diseases and in the process of inflammation, biomineralization, cell viability ...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2018.07.003
更新日期:2018-09-01 00:00:00
abstract::We present data comparing the Roche CEA radioimmunoassay (RIA) utilizing ultrafiltration and the Abbott CEA immunoradiometric assay (IRMA) methods for standard curve sensitivity, analytical reproducibility, recovery (pre and post extraction), dilution linearity, and patient correlation. The Roche intra- and inter-assa...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(82)97007-2
更新日期:1982-10-01 00:00:00
abstract:OBJECTIVES:Analysis of serum/plasma methylmalonic acid (MMA) is important for the diagnosis and management of methylmalonic acidemia in pediatric populations. This work focuses on developing and validating a liquid chromatography tandem mass spectrometry (LC-MS/MS) method to monitor methylmalonic acidemia using a simpl...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2016.05.010
更新日期:2016-09-01 00:00:00
abstract:OBJECTIVES:Hepatitis B virus (HBV) can result in asymptomatic carrier (AsC) state or chronic inflammation of liver, which depends on the host immunity. We therefore investigated the peptidomic profiling in the process of HBV infection. DESIGN AND METHODS:In this study, serum from 116 HBV infected (AsC and chronic hepa...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2013.10.016
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVES:The present study was designed to (a) evaluate and compare plasma ammonia levels (PAL) in patients with acute liver failure (ALF) and chronic liver disease (CLD) with or without hepatic encephalopathy (HE); (b) correlate the severity of HE with PAL; and (c) correlate PAL with clinical features of raised intr...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2005.04.013
更新日期:2005-08-01 00:00:00
abstract:OBJECTIVE:To confirm that the naproxen metabolite O-desmethylnaproxen interferes with the Jendrassik and Grof method for total bilirubin and was a possible cause of spurious hyperbilirubinemia in a case of naproxen overdose. DESIGN AND METHODS:Plasma was spiked with naproxen or O-desmethylnaproxen before total and dir...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2008.09.119
更新日期:2009-01-01 00:00:00
abstract:OBJECTIVE:This study aimed at evaluating Elecsys free triiodothyronine (FT3) assay performed on an Elecsys 2010 system, while paying special attention to age relationship in euthyroid subjects. DESIGN AND METHODS:FT3 levels were measured in 149 euthyroid control subjects aged between 2 and 92 years old, 33 hyperthyroi...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(98)00024-1
更新日期:1998-07-01 00:00:00
abstract::1. The following proteolytic enzymes were measured in muscles of control subjects and patients with muscular dystrophies and related neuromuscular diseases: an elastase-like enzyme, carboxypeptidase A, carboxypeptidase B and pyroglutamyl peptidase. 2. Elastase-like enzyme and carboxypeptidase B did not show significan...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(79)80001-6
更新日期:1979-04-01 00:00:00
abstract:OBJECTIVES:To determine pediatric reference values for serum zinc concentration in Iranian subjects. DESIGN AND METHODS:Serum zinc concentration was measured by flame atomic absorption spectrometry in 699 children and adolescents. Reference values for serum zinc were determined according to the Clinical and Laboratory...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2012.07.086
更新日期:2012-10-01 00:00:00
abstract:CONTEXT:Knee arthroplasty surgery is significant trauma, leading to an activated immune system causing inflammation and oxidative stress. Many current biomarkers are invasive, costly, and often slow to analyse, limiting their use for rapid inflammatory measurements. OBJECTIVES:We have examined the use of urinary neopt...
journal_title:Clinical biochemistry
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1016/j.clinbiochem.2018.11.002
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVES:Biallelic mutations in the ARG1 gene result in an uncommon autosomal recessive inborn defect of the urea cycle known as hyperargininemia (OMIM #207800). ARG1 splicing mutations are not reported often, and they are probably related to a more severe phenotype than missense mutations. In this article, we descri...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2015.07.015
更新日期:2015-12-01 00:00:00
abstract::Fourteen antirheumatoid drugs were tested for their effects on the in vitro hyaluronidase activity of normal human serum. Four drugs produced significant changes in enzyme activity. Different results were obtained with ovine testicular hyaluronidase when diluted with either saline or inactivated human serum. No increa...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(76)80053-7
更新日期:1976-08-01 00:00:00
abstract:OBJECTIVE:Elevated concentrations of hCG beta core fragment (hCGβcf) are known to cause false-negative results in qualitative urine pregnancy test devices, but the pattern of urinary hCGβcf during normal pregnancy has not been well characterized. Here, we evaluate the relationship between urine hCG, hCGβcf, and hCG fre...
journal_title:Clinical biochemistry
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.clinbiochem.2016.04.006
更新日期:2016-07-01 00:00:00
abstract:OBJECTIVE:To review biochemical literature and determine the significance of non-covalent bonding in plasma. RESULTS:Many non-covalent bonds exist in plasma. Relatively strong self-associations are found for serum albumin, several gamma-globulins, apolipoproteins and fibrin(ogen). Here, fibrinogen and fibrin, consider...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(96)00159-2
更新日期:1997-03-01 00:00:00
abstract:OBJECTIVES:Pediatric serum samples collected from healthy children in the CALIPER (Canadian Laboratory Initiative in Pediatric Reference Interval) project are stored at -80 °C for various periods of time. This study aimed to determine the stability of chemistry, protein, and hormone analytes under these conditions. DE...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2012.03.029
更新日期:2012-07-01 00:00:00
abstract:OBJECTIVES:Delayed graft function (DGF) is still a major issue in kidney transplantation. Plasma and urine neutrophil gelatinase-associated lipocalin (NGAL) were evaluated in a population of kidney donors and recipients to investigate their performance to predict early renal function. DESIGN AND METHODS:Plasma (pNGAL)...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2014.06.079
更新日期:2014-10-01 00:00:00
abstract::Erythrocyte morphology, kinematic viscosity, and membrane lipid composition have not been well documented in heterozygous hypobetalipoproteinemia (HBL). In our study of nine subjects with HBL from two kindreds, three presented with schistocytes (3-6%) while two of them also showed acanthocytosis (25-35%) on their peri...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(88)80020-1
更新日期:1988-12-01 00:00:00
abstract:BACKGROUND:Laboratory confirmation of alpha-1-antitrypsin (A1AT) deficiency may be achieved by multiple methods. Here, we compare the relative comprehensiveness and efficiency of pathogenic variant (PV) detection of four different protocols utilized at different diagnostic centres in Canada. METHODS:Diagnostic results...
journal_title:Clinical biochemistry
pub_type: 杂志文章,多中心研究
doi:10.1016/j.clinbiochem.2020.05.001
更新日期:2020-07-01 00:00:00
abstract::To design and deliver high quality, safe and effective products, manufacturers of in vitro diagnostic (IVD) products follow a structured, traceable process for controlling the uncertainty of results reported from their measurement systems. This process and its results however, are not often shared in detail with those...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2018.04.012
更新日期:2018-07-01 00:00:00
abstract:BACKGROUND:Increased levels of circulating endostatin predicts cardiovascular morbidity and impaired kidney function in the general population. The utility of endostatin as a risk marker for mortality in the emergency department (ED) has not been reported. AIM:Our main aim was to study the association between plasma e...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2019.10.004
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVE:We performed a side-by-side comparison between three stain-then-lyse commercially available methods (Ortho-mune Lysing solution, FACS lysing solution and ImmunoPrep reagent system) and a lyse-then-stain method using hypotonic NH4Cl. The major difference between these methods is that only in the latter the ali...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(98)00011-3
更新日期:1998-04-01 00:00:00
abstract::Ethanolamine is a compound that is frequently seen in urinary amino acid analysis. Although there is a single report of increased ethanolamine excretion associated with a distinctive storage disease in two siblings, the significance of ethanolaminuria is not known. We measured urinary ethanolamine/creatinine ratios in...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(88)80084-5
更新日期:1988-10-01 00:00:00
abstract:OBJECTIVES:We emphasize the importance of routine follow-up of subnormal TSH concentrations with QC materials. DESIGN AND METHODS:The functional sensitivity (FS) of the ADVIA Centaur system TSH assay was assessed. We report the values yielded for QC materials in two clinical laboratories. RESULTS:The FS was <0.02 mIU...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2012.04.017
更新日期:2012-10-01 00:00:00
abstract::Plasma membrane-bound 5'-nucleotidase (5'-NT), gamma-glutamyltransferase (gamma-GT) and soluble deoxynucleotidyltransferase (TdT) were studied in peripheral blood cells (PBMN) of 35 individuals, 26 male and 9 female, with circulating anti-HIV antibodies. Twenty-six were drug abusers, 2 were drug abusers and homosexual...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(89)80026-8
更新日期:1989-08-01 00:00:00
abstract:OBJECTIVES:Δ(9)-Tetrahydrocannabinol (THC) can be measured in exhaled breath by using an aerosol particle collection device. The sampling procedure is simple, non-invasive and takes only 2-3min. In the present study we measured the amount of THC in exhaled breath of cannabis users at specific time intervals up to 3h af...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2016.06.003
更新日期:2016-09-01 00:00:00