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Cortisol and cortisone analysis in serum and plasma by atmospheric pressure photoionization tandem mass spectrometry.

Abstract：

OBJECTIVES:Cortisol metabolism is controlled by 11beta-hydroxysteroid dehydrogenase (11beta-HSD) isoenzymes, which interconvert cortisol and cortisone. Accurate measurement of the cortisol and cortisone concentrations and their ratio provide useful information about 11beta-HSD activity. METHODS:Cortisol and cortisone were extracted with methyl-tert-butyl ether from 100 microl of serum or plasma. The extract was evaporated, reconstituted with mobile phase, and analyzed by tandem mass spectrometry using a photoionization interface. The transitions monitored were: m/z 363 to 121 and 363 to 97 for cortisol, 361 to 163 and 361 to 105 for cortisone. RESULTS:Within-run and between-run coefficients of variation were less than 6% and 12%; 14% and 22%; 11% and 21% for cortisol, cortisone, and their ratio, respectively. The limit of detection was 1 microg/l for cortisol and 5 microg/l for cortisone. Normal ranges for cortisol and cortisone concentration and for their ratio in plasma (n = 120) determined as the central 95% were 33-246 microg/l for cortisol, 8-27 microg/l for cortisone, and 0.081-0.301 for the cortisone/cortisol ratio. CONCLUSIONS:We developed a simple sensitive method for cortisol and cortisone analysis in plasma and serum that uses a small sample volume. The method is very specific, fast, does not have any known interference, and is useful for diagnosis of variety of disease and pathologic conditions.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Kushnir MM,Neilson R,Roberts WL,Rockwood AL

doi

10.1016/j.clinbiochem.2004.01.005

subject

Has Abstract

pub_date

2004-05-01 00:00:00

pages

357-62

issue

5

eissn

0009-9120

issn

1873-2933

pii

S0009912004000232

journal_volume

37

pub_type

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