Abstract：
:Thyroxine binding protein characteristics were defined in 6 normal subjects, 4 with thyroxine binding globulin (TBG) deficiency, 3 with TBG increase, one with hyperthyroxinemic dysalbuminemia, 7 with severe non-thyroidal illness, and 3 with chronic renal failure. Free thyroxine was measured by Sephadex partition in plasma to which increasing thyroxine concentrations were added. Deconvolution of the resultant titration data was performed by computer modelling. Abnormalities of thyroxine binding capacities or of binding affinities occur in non-thyroidal illness, chronic renal failure, and sporadically. The patient with hyperthyroxinemic dysalbuminemia had increased thyroxine binding affinity to thyroxine binding prealbumin as well as to albumin. "Free-T4 assays" or estimates of Free-T4 by calculation from total thyroxine and measures of protein binding such as T3-uptake must be expected to be perturbed by these binding protein abnormalities unless such interferences are explicitly demonstrated to be absent.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Keane PM,Walker WH,Thornton G,Rodbard Ddoi
10.1016/s0009-9120(86)80073-xsubject
Has Abstractpub_date
1986-02-01 00:00:00pages
52-7issue
1eissn
0009-9120issn
1873-2933pii
S0009-9120(86)80073-Xjournal_volume
19pub_type
杂志文章abstract：BACKGROUND:It is unclear if the point-of-care (POC) Clinitest hCG device is subject to high-dose hook interference from physiological concentrations of intact human chorionic gonadotropin (hCG), β-core fragment of hCG (hCGβcf), and hCG free β-subunit (hCGβ) found in urine during pregnancy. We used a simulation model to...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2014.11.004
更新日期：2015-02-01 00:00:00
abstract：OBJECTIVES:To verify the role of IL-12 in the pathogenesis of psoriasis and determine its relation to IFNgamma. DESIGN AND METHODS:Skin biopsies from lesional and non-lesional skin of 30 patients and 10 healthy controls were obtained for quantitative PCR examination of IL-12 (P40) and IFNgamma mRNA as well as in situ ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2005.11.008
更新日期：2006-02-01 00:00:00
abstract：OBJECTIVES:To study the usefulness of combined cardiac Troponin T (cTnT) and CK-MB mass determinations in risk stratification of acute coronary syndromes. DESIGN AND METHODS:Blood samples for cTnT and CK-MB mass were collected at arrival and 4, 8, and 12-24 later in 301 consecutive patients with recent acute chest pai...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2003.12.003
更新日期：2004-04-01 00:00:00
abstract：:Creatine kinase (CK) isoenzymes were determined on 15 patients undergoing open heart surgery with bypass. The study was performed to examine the relationship between the hypothermia under which the surgery is conducted and the appearance of CK isoenzymes in the serum both during and after surgery. All patients showed ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(82)96822-9
更新日期：1982-12-01 00:00:00
abstract：OBJECTIVES:The circulating lipoproteins may cause some abnormalities in platelet composition and function in hypercholesterolemia. The aim of this study was to investigate whether platelet apoptosis, platelet activation, platelet aggregation, platelet-leukocyte aggregate (PLA) formation and lipid peroxidation occur sim...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2005.09.005
更新日期：2005-12-01 00:00:00
abstract：:A study conducted on 228 diabetic patients has shown a significant positive association between serum gamma-glutamyl transpeptidase (GGT) and triglyceride levels. Both fall with treatment, the most marked reduction occurring in patients on insulin. We suggest that the association between serum GGT and triglyceride lev...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(76)80059-8
更新日期：1976-08-01 00:00:00
abstract：OBJECTIVES:Molecular screening plays a major role in prognostic categorization and subsequent definition of treatment strategies for acute myeloid leukemia. The possibility of using IDH1/2 mutations as a marker for the monitoring of minimal residual disease (MRD) is still under investigation and remains unclear. METHO...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2018.08.012
更新日期：2018-11-01 00:00:00
abstract：:We present data comparing the Roche CEA radioimmunoassay (RIA) utilizing ultrafiltration and the Abbott CEA immunoradiometric assay (IRMA) methods for standard curve sensitivity, analytical reproducibility, recovery (pre and post extraction), dilution linearity, and patient correlation. The Roche intra- and inter-assa...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(82)97007-2
更新日期：1982-10-01 00:00:00
abstract：AIM:Luciferase immunoprecipitation (LIPS) assays show good sensitivity and specificity in testing islet specific autoantibodies for diagnosis of type 1 diabetes (T1D). However, there are currently no LIPS assays available for detecting proinsulin/insulin autoantibody (IAA) previously. We here developed a LIPS assay to ...
journal_title：Clinical biochemistry
pub_type： 临床试验,杂志文章
doi：10.1016/j.clinbiochem.2018.02.011
更新日期：2018-04-01 00:00:00
abstract：:Human liver arginase has many biological effects on lymphocytes, macrophages, liver cells, and tumor cells, in addition to its major role in the liver urea cycle. We have developed a sandwich enzyme-linked immunosorbent assay (ELISA) method to quantitate arginase concentrations in plasma that can be applied to various...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(93)80009-j
更新日期：1993-12-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to develop an improved method of direct DNA sequencing, which makes it possible to identify heterozygous mutations of the lipoprotein lipase (LPL) gene in order to understand the underlying genetic disorder of type IV hyperlipoproteinemia. METHODS AND RESULTS:The direct sequenci...
journal_title：Clinical biochemistry
pub_type： 临床试验,杂志文章
doi：10.1016/s0009-9120(97)00005-2
更新日期：1997-06-01 00:00:00
abstract：OBJECTIVE:Immunosuppressive therapy after liver transplantation may be a risk for kidney dysfunction. This work was designed to determine whether Tamm-Horsfall Protein (THP) could be considered as a marker for nephrotoxicity. DESIGN AND METHODS:THP was determined by an ELISA method in serial 24-h urine from liver tran...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(02)00274-6
更新日期：2002-02-01 00:00:00
abstract：BACKGROUND:Numerous etiologies are implicated in the complications of diabetes. A link between diabetes and the predisposition to certain cancers, including colon cancer, has been established during the last decade. However, the relationship between fecal occult blood test (FOBT), a screening method used principally fo...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2016.10.005
更新日期：2017-01-01 00:00:00
abstract：OBJECTIVES:To assess the medical applicability of CpG methylation as molecular markers for cancer diagnosis, we established a new system to determine DNA methylation based on TaqMan PCR combined with a methyl-binding-domain polypeptide 2. DESIGN AND METHODS:We evaluated the diagnostic applicability of this approach by...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2009.03.017
更新日期：2009-07-01 00:00:00
abstract：OBJECTIVES:The ratio of glycated albumin to HbA1c (GA/HbA1c ratio) is a known indicator that reflects fluctuations in plasma glucose. In this study, the association of the GA/HbA1c ratio to diabetic nephropathy and diabetic retinopathy in patients with type 2 diabetes was investigated. DESIGN AND METHODS:Among patient...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2016.11.032
更新日期：2017-04-01 00:00:00
abstract：BACKGROUND:Circulating B-type natriuretic peptide (BNP) is widely accepted as a diagnostic and risk assessment biomarker of cardiac function. Studies suggest that there are significant differences in measured concentrations among different commercial BNP immunoassays. The purpose of our study was to compare BNP-related...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2016.11.003
更新日期：2017-03-01 00:00:00
abstract：OBJECTIVES:The ability of current immunoassays to accurately measure equimolar amounts of 25(OH)D2 and 25(OH)D3 has been recently questioned. This study determined serum 25(OH)D2, 25(OH)D3 and total serum 25(OH)D concentrations in healthy vitamin D2-supplemented subjects by isotope dilution liquid chromatography mass s...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2015.05.021
更新日期：2015-11-01 00:00:00
abstract：BACKGROUND:Colorectal cancer (CRC) is one of the most common cancers worldwide, and emerging lines of evidence have implicated circular RNAs (circRNAs), a novel class of endogenous noncoding RNAs, in CRC development. However, whether plasma circRNAs might be novel diagnostic biomarkers for CRC remains unclear. METHODS...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2019.10.012
更新日期：2019-12-01 00:00:00
abstract：UNLABELLED:Frozen biospecimens are crucial for translational research and contain well-preserved nucleic acids and protein. However, the risks of freezer failure as well as space, cost, and environmental concerns of frozen biospecimens are substantial. OBJECTIVE:The purpose of the study was to review the current statu...
journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.clinbiochem.2013.12.011
更新日期：2014-03-01 00:00:00
abstract：OBJECTIVE:To establish a ELISA assay to measure release of type 1-phospholipase A2 propeptide from activated granulocytes. Human type 1-prophospholipase A2 (1-proPLA2) is biosynthesized and stored as inactive zymogen. Activation involves tryptic-like cleavage at the N-terminus, with equimolar release of the heptapeptid...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(94)00068-7
更新日期：1995-02-01 00:00:00
abstract：BACKGROUND:Single biomarker approaches provide only moderate accuracy in the non-invasive detection of exercise-induced myocardial ischemia. We therefore assessed the combination of the two most promising single biomarkers: high-sensitivity cardiac troponin I (hs-cTnI) and B-type natriuretic peptide (BNP). METHODS:Con...
journal_title：Clinical biochemistry
pub_type： 临床试验,杂志文章
doi：10.1016/j.clinbiochem.2017.10.014
更新日期：2018-02-01 00:00:00
abstract：OBJECTIVES:To examine whether the proportions of blood leukocyte subsets are altered in COPD, and to assess the disturbances in signalling pathways in the leukocytes of COPD patients, with respect to smoking history. DESIGN AND METHODS:The study was performed at the Faculty of Pharmacy and Biochemistry and University ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2007.12.013
更新日期：2008-04-01 00:00:00
abstract：BACKGROUND:Therapeutic drug monitoring (TDM) of the widely prescribed antidepressant fluoxetine (FLU) is recommended in certain situations, such as occurrence of toxicity, inadequate response or suspect of poor adherence. Dried blood spot (DBS) sampling is an increasingly studied alternative for TDM, particularly for o...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2017.10.002
更新日期：2018-02-01 00:00:00
abstract：OBJECTIVES:To evaluate the analytical performance of the testosterone assay performed on the Bayer immuno 1 system from Bayer Corporation. DESIGN AND METHODS:The precision was measured using three Bayer TESTpoint Ligand controls, three Medical Decision Pools and the Bayer SETpoint Testosterone calibrators. The lineari...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(97)00140-9
更新日期：1998-02-01 00:00:00
abstract：:The recently developed fluorescent immunoassay (FIA) for measuring serum carbamazepine concentration is compared to the established enzyme-multiplied-immunoassay-technique (EMIT). The Accuracy, precision and simplicity of these methods are comparable. The sensitivity of FIA is approximately eight times greater than th...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(82)96854-0
更新日期：1982-12-01 00:00:00
abstract：:Three commercially available assays (an enzyme-linked immunosorbent assay ELISA, an immunoradiometric assay, IRMA, and a nephelometric assay) for the determination of lipoprotein(a) [Lp(a)] were compared with respect to the dependency of these assays on the various apolipoprotein(a) [apo(a)] isoforms. Although there w...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(94)00052-w
更新日期：1994-12-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to determine whether the atherogenic index of plasma (AIP=log[triglycerides/HDL-cholesterol]) differs in heterozygous familial hypercholesterolemia (FH) patients with and without a history of cardiovascular disease (CVD). DESIGN AND METHODS:A total of 555 FH patients with known mut...
journal_title：Clinical biochemistry
pub_type： 杂志文章,多中心研究
doi：10.1016/j.clinbiochem.2011.11.001
更新日期：2012-01-01 00:00:00
abstract：:This study was performed to investigate the antioxidant mechanisms of polymorphonuclear leucocytes (PMN) in active stage of Behçet's Disease. PMN activities of myeloperoxidase (p < 0.02), superoxide dismutase (p < 0.001), catalase (p < 0.005), and glutathione peroxidase (p < 0.005) were significantly lower in the pati...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(94)90046-9
更新日期：1994-10-01 00:00:00
abstract：OBJECTIVES:To establish whether normal human subjects excrete glycine betaine at a constant rate. DESIGN AND METHODS:Urine was collected from ten normal healthy male subjects for 14 days, during which fluid intake was systematically varied from <800 mL to >3 L per day. Glycine betaine, sorbitol and creatinine excretio...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2006.10.029
更新日期：2007-04-01 00:00:00
abstract：:The synthesis and characterisation of a new colorimetric reagent for iron, 3-(2-pyridyl)-5,6-bis(2-furyl)-1,2,4-triazine are described. Peak assignments and chemical shifts of the high resolution 13C nmr spectrum are given. The reagent forms a tris iron(II) complex with epsilon max of 32000 Lcm-1 mol-1 at 577 nm. The ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(84)90613-1
更新日期：1984-10-01 00:00:00
