当前位置： SCI文献检索 > CLINICAL BIOCHEMISTRY期刊下所有文献 > Plasma circular RNA panel acts as a novel diagnostic biomarker for colorectal cancer.

Plasma circular RNA panel acts as a novel diagnostic biomarker for colorectal cancer.

Abstract：

BACKGROUND:Colorectal cancer (CRC) is one of the most common cancers worldwide, and emerging lines of evidence have implicated circular RNAs (circRNAs), a novel class of endogenous noncoding RNAs, in CRC development. However, whether plasma circRNAs might be novel diagnostic biomarkers for CRC remains unclear. METHODS:We investigated the plasma levels of selected circRNAs by quantitative real-time PCR (qRT-PCR). The presence of the candidate circRNAs was confirmed through RNase R assays, qRT-PCR and DNA sequencing, and their diagnostic value was evaluated using a receiver operating characteristic (ROC) curve. RESULTS:The plasma levels of three circRNAs (circ-CCDC66, circ-ABCC1 and circ-STIL) were significantly decreased in CRC patients (n = 45) compared with healthy controls (n = 61). The ROC curve analysis showed that the area under the ROC curve (AUC) of the three-circRNA panel was 0.780, which is higher than that of traditional protein biomarkers, such as carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9). Combining the circRNA panel with CEA and CA19-9 might improve the ability to diagnose CRC (AUC = 0.855). In addition, the plasma circ-ABCC1 level was related to tumor growth and progression, and the plasma circ-CCDC66 and circ-ABCC1 levels were decreased in precursor lesions of CRC, including colon adenomas and adenomatous polyps. More importantly, circ-CCDC66 and circ-STIL were found to be useful for diagnosing early-stage CRC, and the three-circRNA panel improved the ability to diagnose CEA-negative and CA19-9-negative CRC. CONCLUSION:Our study provides the first identification of a panel of three plasma circRNAs that could serve as a novel and independent diagnostic biomarker for CRC.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Lin J,Cai D,Li W,Yu T,Mao H,Jiang S,Xiao B

doi

10.1016/j.clinbiochem.2019.10.012

subject

Has Abstract

pub_date

2019-12-01 00:00:00

pages

60-68

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(19)30541-7

journal_volume

74

pub_type

杂志文章

相关文献

CLINICAL BIOCHEMISTRY文献大全
  • Functional polymorphism in the manganese superoxide dismutase (MnSOD) gene in patients with asthma.

    abstract：OBJECTIVES:To investigate the possible association between functional variant Ala-9Val in the MnSOD gene and asthma in the case-control study comprising 626 Caucasian subjects. METHODS:MnSOD genotypes were determined by PCR with subsequent restriction analysis by the BsaWI enzyme. RESULTS:Significant differences in a...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2005.12.001

    authors： Holla LI,Kankova K,Vasku A

    更新日期：2006-03-01 00:00:00

  • A novel (A)γδβ(0)-thalassemia caused by DNA deletion-inversion-insertion of the β-globin gene cluster and five olfactory receptor genes: Genetic interactions, hematological phenotypes and molecular characterization.

    abstract：OBJECTIVE:To report the phenotypes and genetic basis of a novel (A)γδβ(0)-thalassemia found in Thai individuals with several forms of thalassemia. DESIGNS AND METHODS:An initial study was done in an adult Thai woman who had hypochromic microcytic red cells with unusually 100% Hb F. Extended study was carried out on he...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2015.03.023

    authors： Singha K,Fucharoen G,Hama A,Fucharoen S

    更新日期：2015-07-01 00:00:00

  • The diagnostic value of apolipoprotein E in pediatric patients with invasive bacterial infections.

    abstract：OBJECTIVE:To evaluate the potential diagnostic value of apolipoprotein E (ApoE) measurements in pediatric patients with invasive bacterial infections. DESIGN AND METHOD:A total of 185 pediatric patients were enrolled in this study, including 94 patients with confirmable infections and 91 patients without confirmable i...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2011.11.009

    authors： Wang C,Wang Y,Wang A,Fu P,Yang Y

    更新日期：2012-02-01 00:00:00

  • A reference system for cortisol.

    abstract：OBJECTIVES:The International Federation of Clinical Chemistry (IFCC) initiated a pilot study, with cortisol as an example, that aims to implement the concept of standardization of hapten immunoprocedures on the basis of metrological traceability. In fact, this standardization concept comes down to correct calibration o...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(98)00037-x

    authors： Thienpont LM

    更新日期：1998-08-01 00:00:00

  • Correlation of endothelin 1 plasma levels with plasma antioxidant capacity in elderly patients treated for hypertension.

    abstract：UNLABELLED:Experimental studies confirmed that reactive oxygen species increase endothelin-1 (ET-1) synthesis, and modulate ET-1 signaling pathway resulting in vasoconstriction and vascular remodeling. The aim of this study was to evaluate the relationship between plasma ET-1 concentration and antioxidant status in pat...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2008.11.002

    authors： Skalska AB,Pietrzycka A,Stepniewski M

    更新日期：2009-03-01 00:00:00

  • Quality assurance practices for point of care testing programs: Recommendations by the Canadian society of clinical chemists point of care testing interest group.

    abstract：:Point of Care Testing (POCT) refers to clinical laboratory testing performed outside the central laboratory, nearer to the patient and sometimes at the patient bedside. The testing is usually performed by clinical staff, such as physicians or nurses, who are not laboratory trained. This document was developed by the P...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.clinbiochem.2020.11.008

    authors： Venner AA,Beach LA,Shea JL,Knauer MJ,Huang Y,Fung AWS,Dalton J,Provencal M,Shaw JLV

    更新日期：2021-02-01 00:00:00

  • Serum galectin-3 levels in patients with nonalcoholic fatty liver disease.

    abstract：OBJECTIVES:Galectin-3 might serve as a biomarker of human metabolic alterations. We measured serum levels of galectin-3 in patients with nonalcoholic fatty liver disease (NAFLD) and examined their association with clinical and histological phenotypes. DESIGN AND METHODS:Serum levels of galectin-3 were assayed in 71 pa...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2011.05.015

    authors： Yilmaz Y,Eren F,Kurt R,Yonal O,Polat Z,Senates E,Bacha M,Imeryuz N

    更新日期：2011-08-01 00:00:00

  • Estimation of LDL cholesterol based on the Friedewald formula and on apo B levels.

    abstract：OBJECTIVES:The plasma apolipoprotein B (apo B) concentrations have been considered to be a more accurate representation of atherogenic particles and it has been proposed that the formula LDL-C (mmol/L) = 0.41TC - 0.32TG + 1.70apo B - 0.27 is reliable for the estimation of LDL-C (Clin Chem 1997; 43: 808-15). We undertoo...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(00)00162-4

    authors： Bairaktari E,Hatzidimou K,Tzallas C,Vini M,Katsaraki A,Tselepis A,Elisaf M,Tsolas O

    更新日期：2000-10-01 00:00:00

  • Glucose-6-phosphate dehydrogenase enzyme stability in filter paper dried blood spots.

    abstract：OBJECTIVE:Prior to initial distribution of Glucose-6-phosphate dehydrogenase (G6PD) proficiency testing (PT) materials, we evaluated G6PD enzyme stability in dried blood spots (DBS) under various temperature and humidity environments to develop storage and usage guidelines for our new materials. DESIGN & METHODS:We pr...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2017.05.003

    authors： Flores SR,Hall EM,De Jesús VR

    更新日期：2017-10-01 00:00:00

  • A convenient method for the determination of non-specific binding in commercially available solid phase iodine labeled radioimmunoassay kits.

    abstract：:57Co-cyanocobalamin is substituted for iodine labeled antigen in three commercially available solid phase radioimmunoassay kits to evaluate the extent of non-specific binding by determination of the supernatant and residual fraction of unbound ligand and thus obtain a value for corrected binding throughout the range o...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(78)90070-x

    authors： Carter P

    更新日期：1978-06-01 00:00:00

  • 1,5-anhydroglucitol monitoring in diabetes: a mass balance perspective.

    abstract：:1,5-anhydroglucitol (AG) is a nonmetabolizable glucose analogue found in plasma due to ingestion. The normal steady-state concentration can be dramatically decreased by inhibition of tubular reabsorption during periods of hyperglycemia. For this reason, monitoring of AG has been plausibly advocated for detection of pe...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.clinbiochem.2008.08.086

    authors： Nerby CL,Stickle DF

    更新日期：2009-02-01 00:00:00

  • Performance evaluation of four 25-hydroxyvitamin D assays to measure 25-hydroxyvitamin D2.

    abstract：OBJECTIVES:The ability of current immunoassays to accurately measure equimolar amounts of 25(OH)D2 and 25(OH)D3 has been recently questioned. This study determined serum 25(OH)D2, 25(OH)D3 and total serum 25(OH)D concentrations in healthy vitamin D2-supplemented subjects by isotope dilution liquid chromatography mass s...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2015.05.021

    authors： Freeman J,Wilson K,Spears R,Shalhoub V,Sibley P

    更新日期：2015-11-01 00:00:00

  • Simplified method for determination of protocollagen proline hydroxylase.

    abstract：:Preparation of (14C) Pro labeled protocollagen and requirements for its hydroxylation by PPH was investigated. Protocollagen can be prepared by centrifugation of the biological material at 15.000 X g. Substances in current use, viz catalase, bovine serum albumin and dithiothreitol were found unnecessary under the hydr...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(76)80072-0

    authors： Blumenkrantz N,Asboe-Hansen G

    更新日期：1976-12-01 00:00:00

  • The value of biliary amylase and Hepatocarcinoma-Intestine-Pancreas/Pancreatitis-associated Protein I (HIP/PAP-I) in diagnosing biliary malignancies.

    abstract：BACKGROUND/OBJECTIVES:Elevated concentrations of Hepatocarcinoma-Intestine-Pancreas/Pancreatitis-associated Protein I (HIP/PAP-I) in pancreatic juice have been reported in patients with pancreatic adenocarcinoma and have been considered as a promising tumor marker. This study was conducted to investigate whether biliar...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2005.01.012

    authors： Chen CY,Lin XZ,Wu HC,Shiesh SC

    更新日期：2005-06-01 00:00:00

  • Comparison of new solid-phase extraction methods for chromatographic identification of drugs in clinical toxicological analysis.

    abstract：OBJECTIVES:Newly presented solid-phase extraction methods (Solid Phase Disc Extraction, SPEC-Plus, Multi-Modal, and Solid Phase Micro Extraction, SPME have been checked with respect to their applicability to clinical toxicological analysis. In comparison with conventional liquid/liquid-extraction and a common mixed-pha...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(96)00096-3

    authors： Degel F

    更新日期：1996-12-01 00:00:00

  • The shift from fluoride/oxalate to acid citrate/fluoride blood collection tubes for glucose testing - the impact upon patient results.

    abstract：OBJECTIVES:To ascertain whether or not the change in blood collection tubes for plasma glucose from fluoride/oxalate to citrate/fluoride/EDTA has had an effect upon the glucose results. METHODS:Plasma glucose results from fasting patients from 2007 to 2012 were extracted from the laboratory information system. The dat...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2014.01.011

    authors： Norman M,Jones I

    更新日期：2014-05-01 00:00:00

  • Vitamin D-binding protein gene polymorphism association with IA-2 autoantibodies in type 1 diabetes.

    abstract：BACKGROUND:Vitamin D-binding protein (DBP) is the main systemic transporter of 1.25(OH)2D3 and is essential for its cellular endocytosis. There are two known polymorphisms in exon 11 of the DBP gene resulting in amino acid variants: GAT-->GAG substitution replaces aspartic acid by glutamic acid in codon 416; and ACG-->...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2004.12.013

    authors： Ongagna JC,Pinget M,Belcourt A

    更新日期：2005-05-01 00:00:00

  • Effects of lifestyle modification on oxidized LDL, reactive oxygen species production and endothelial cell viability in patients with coronary artery disease.

    abstract：OBJECTIVES:We evaluated the effects of lifestyle modification (LM) on lipid profile, oxidative stress and serum-stimulated human coronary artery endothelial cell (HCAEC) viability in coronary artery disease (CAD) patients after 6months. DESIGN AND METHODS:Thirty patients with CAD were randomly assigned to LM intervent...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.clinbiochem.2010.04.056

    authors： Srimahachota S,Wunsuwan R,Siritantikorn A,Boonla C,Chaiwongkarjohn S,Tosukhowong P

    更新日期：2010-07-01 00:00:00

  • Multiple atherosclerosis-related biomarkers associated with short- and long-term mortality after stroke.

    abstract：OBJECTIVES:We investigated the relationships of biomarkers of various pathophysiologic pathways including high-sensitivity C-reactive protein (hs-CRP), lipocalin-2 (LCN2), myeloperoxidase (MPO) and matrix metalloproteinases 9 (MMP9) with mortality in stroke patients. DESIGN AND METHODS:hs-CRP, LCN2 and MPO concentrati...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2012.06.014

    authors： Chan CP,Jiang HL,Leung LY,Wan WM,Cheng NM,Ip WS,Cheung KY,Chan RW,Wong LK,Graham CA,Renneberg R,Rainer TH

    更新日期：2012-11-01 00:00:00

  • Recent developments in meconium and hair testing methods for the confirmation of gestational exposures to alcohol and tobacco smoke.

    abstract：:The use of alcohol and tobacco is prevalent among pregnant women despite the well-known adverse effects of these substances imposed on the developing fetus and immense public health education efforts. Confirmation of gestational exposures to these compounds have relied mostly on maternal self-reporting, which is often...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.clinbiochem.2004.01.010

    authors： Chan D,Caprara D,Blanchette P,Klein J,Koren G

    更新日期：2004-06-01 00:00:00

  • Preanalytic of total antioxidant capacity assays performed in serum, plasma, urine and saliva.

    abstract：:The investigation of oxidative stress (OS), its mechanisms and connections with human diseases, is a topic of interest with more than 36,000 PubMed citations to date. The OS can be approached either from the perspective of pro-oxidation, either of anti-oxidation, and both can be investigated considering individual che...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.clinbiochem.2016.11.037

    authors： Ialongo C

    更新日期：2017-04-01 00:00:00

  • Comparison of gas-liquid chromatography and EMIT assay for serum valproic acid.

    abstract：:We describe a simple direct extraction method for the gas-liquid chromatography determination of serum valproic acid. The working range for the assay is 2-180 mg/L and our within-run precision was 5.8 and 4.3% at the 40 and 90 mg/L concentrations respectively. Hemolyzed and lipemic sera as well as samples from patient...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(81)90772-4

    authors： Leroux M,Budnik D,Hall K,Irvine-Meek J,Otten N,Seshia S

    更新日期：1981-04-01 00:00:00

  • Amplification of intermediate-size DNA sequences from formalin and B-5 fixed tissue by polymerase chain reaction.

    abstract：:Retrospective analysis of DNA from paraffin-embedded fixed bone marrow biopsy specimens is possible if preceded by amplification of the DNA sequences of interest by the polymerase chain reaction (PCR). These fixed specimens yield degraded DNA that may not be suitable for direct analysis by conventional digestion and h...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/0009-9120(92)80051-h

    authors： Crisan D,Mattson JC

    更新日期：1992-04-01 00:00:00

  • Hyaluronic acid as a non-invasive biomarker of liver fibrosis.

    abstract：UNLABELLED:Chronic liver diseases may cause inflammation and progressive scarring, over time leading to irreversible hepatic damage (cirrhosis). As a result, the need to assess and closely monitor individuals for risk factors of components of matrix deposition and degradation, as well as the severity of the fibrosis us...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.clinbiochem.2015.07.019

    authors： Neuman MG,Cohen LB,Nanau RM

    更新日期：2016-02-01 00:00:00

  • Analysis of the effects of iron and vitamin C co-supplementation on oxidative damage, antioxidant response and inflammation in THP-1 macrophages.

    abstract：OBJECTIVES:The aims of the study were to test the susceptibility of THP-1 macrophages to develop oxidative stress and to deploy antioxidant defense mechanisms that insure the balance between the pro- and antioxidant molecules. DESIGN AND METHODS:Differentiated THP-1 were incubated in the presence or absence of iron-as...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2011.04.012

    authors： Marcil V,Lavoie JC,Emonnot L,Seidman E,Levy E

    更新日期：2011-07-01 00:00:00

  • Systemic inflammation up-regulates serum hepcidin in exacerbations and stabile chronic obstructive pulmonary disease.

    abstract：OBJECTIVES:Hepcidin is the main regulator of systemic iron homeostasis and its expression is modulated by iron status, hypoxia, erythroid factors and inflammation. The aim of our study was to examine a relationship between level of hepcidin and iron status, erythropoietic activity, hypoxia and inflammation in exacerbat...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2015.07.010

    authors： Tandara L,Grubisic TZ,Ivan G,Jurisic Z,Tandara M,Gugo K,Mladinov S,Salamunic I

    更新日期：2015-12-01 00:00:00

  • Enhanced reduction in oxidative stress and altered glutathione and thioredoxin system response to unsaturated fatty acid load in familial hypercholesterolemia.

    abstract：OBJECTIVES:Familial hypercholesterolemia (FH) is characterized by increased oxidative stress (OS) levels. In the postprandial state, lipids and lipoproteins modulate OS status through their impact on pro-oxidant and antioxidant mechanisms. The objective of this study was to evaluate in patients with FH the response to ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2014.09.006

    authors： Cortes R,Martinez-Hervas S,Ivorra C,De Marco G,Gonzalez-Albert V,Rojo-Martínez G,Saez G,Carmena R,Ascaso JF,Real JT,Chaves FJ

    更新日期：2014-12-01 00:00:00

  • Changes in advanced oxidation protein products, advanced glycation end products, and s-nitrosylated proteins, in patients affected by polycythemia vera and essential thrombocythemia.

    abstract：OBJECTIVES:Oxidative stress has a clear pro tumoral effect in myeloproliferative neoplasms (MPDs). In this study, we analyzed oxidative stress in patients with essential thrombocythemia (ET) and polycythemia vera (PV). Design and methods We analyzed serum levels of advanced oxidation protein products (AOPPs) degradatio...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2012.07.100

    authors： Musolino C,Allegra A,Saija A,Alonci A,Russo S,Spatari G,Penna G,Gerace D,Cristani M,David A,Saitta S,Gangemi S

    更新日期：2012-11-01 00:00:00

  • Inherited factor V deficient neonate with galactosaemia.

    abstract：OBJECTIVES:Reporting a case of inherited factor V deficiency and galactosemia. METHODS:A neonate was admitted with hematoma, jaundice, splenomegaly, diarrhea, anemia, abdominal ascites and bilateral cataracts that diagnosis of galactosaemia and factor V deficiency was established. RESULTS:Coinheritance of both coagul...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.clinbiochem.2011.12.024

    authors： Mansouritorghabeh H,Sharifi-Hoseini MR,Shahroudian M

    更新日期：2012-03-01 00:00:00

  • Effect of eating on plasma radical-trapping antioxidant activity (TRAP) in patients with cirrhosis.

    abstract：OBJECTIVES:To ascertain the effects of eating on plasma antioxidant capacity in patients with liver disease. DESIGN AND METHODS:Eighteen cirrhotic patients were compared to 18 age and sex-matched controls. TRAP was measured by a fluorometric assay after a 12 h fast, and 60, 120, and 180 min after the study participant...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(99)00039-9

    authors： Pirisi M,Federico E,Bortolotti N,Fabris C,Falleti E,Soardo G,Gonano F,Bartoli E

    更新日期：1999-08-01 00:00:00