Abstract：
:We present data comparing the Roche CEA radioimmunoassay (RIA) utilizing ultrafiltration and the Abbott CEA immunoradiometric assay (IRMA) methods for standard curve sensitivity, analytical reproducibility, recovery (pre and post extraction), dilution linearity, and patient correlation. The Roche intra- and inter-assay precision figures for this assay were 8% and 15%, respectively, for a CEA concentration of 4.0 ng/mL. The Abbott assay gave comparable precision values of 9% and 16%, respectively, for a CEA concentration of 2.0 ng/mL. Recovery for plasma spiked with CEA stock preparations was commercial-source-dependent when assayed by the Roche assay, but independent when measured by the Abbott assay. The Roche assay did not recover both preparations quantitatively, while the Abbott assay did. Plasma dilution studies over a wide range of CEA concentrations gave linear results for both assays provided the Roche assay utilizes the extraction method with dialyzate dilution for CEA values greater than 20 ng/mL. A difference (60%) was observed in linear regression slopes of the measured values between the Roche "indirect" and "direct" assays. Exposure of CEA to perchloric acid seems to play a critical role in recovery of CEA material when assayed by the Roche method. A large number of the patient samples assayed gave widely discrepant results when comparing the RIA to the IRMA methods. The potential significance of these discrepancies is discussed.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Valdes R Jr,Stein KEdoi
10.1016/s0009-9120(82)97007-2subject
Has Abstractpub_date
1982-10-01 00:00:00pages
241-7issue
5eissn
0009-9120issn
1873-2933pii
S0009-9120(82)97007-2journal_volume
15pub_type
杂志文章abstract：:We report a case of metastatic adenocarcinoma of the prostate with widespread bony and hepatic involvement having extremely high BB isoenzyme of creatine kinase in serum (2405 U/liter). The serum LD5/LD1 ratio is greater than one, with total LD activity more than twenty-five fold the normal value. The homogenate of pr...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(80)90502-0
更新日期：1980-02-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to determine whether the atherogenic index of plasma (AIP=log[triglycerides/HDL-cholesterol]) differs in heterozygous familial hypercholesterolemia (FH) patients with and without a history of cardiovascular disease (CVD). DESIGN AND METHODS:A total of 555 FH patients with known mut...
journal_title：Clinical biochemistry
pub_type： 杂志文章,多中心研究
doi：10.1016/j.clinbiochem.2011.11.001
更新日期：2012-01-01 00:00:00
abstract：:A study conducted on 228 diabetic patients has shown a significant positive association between serum gamma-glutamyl transpeptidase (GGT) and triglyceride levels. Both fall with treatment, the most marked reduction occurring in patients on insulin. We suggest that the association between serum GGT and triglyceride lev...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(76)80059-8
更新日期：1976-08-01 00:00:00
abstract：OBJECTIVES:Oxidative stress plays a role in the pathogenesis in patients with HCV infection. The objective of this study was to evaluate oxidant and antioxidant biomarkers in patients with HCV. DESIGN AND METHODS:Serum malonaldehyde (MDA) and nitric oxide (NO) levels and the activities of myeloperoxidase (MPO), aryles...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2009.06.007
更新日期：2009-09-01 00:00:00
abstract：OBJECTIVE:This article will review the advancements and new developments being made in (1) advanced computers, (2) microtechnology, (3) advanced immunodiagnostics, (4) neural networks, and (5) molecular biology. The influence of these technologies and their products on clinical laboratories is also discussed. CONCLUSI...
journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/0009-9120(94)00075-7
更新日期：1995-06-01 00:00:00
abstract：:A fluorescence polarization (FP) technique was applied to the assay of lysozyme activity in human urine. In this assay system, reaction between enzyme and fluorescein-labelled substrate produced a decrease in the fluorescein polarization (P) value. Enzyme activity and rate of decrease of P value exhibited a hyperbolic...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(85)80022-9
更新日期：1985-02-01 00:00:00
abstract：OBJECTIVES:We checked the efficiency of a new dialysis device adapted to small volumes to remove glycerol from cryopreserved red blood cells. DESIGN AND METHODS:Dialysis was performed on D-Tube96™ Dialyzer Mini device. In a preliminary trial, we measured the residual glycerol before, and 2, 4 and 24 h after dialysis. ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2011.03.023
更新日期：2011-06-01 00:00:00
abstract：OBJECTIVES:Therapeutic monitoring of everolimus with chromatographic methods (HPLC) enabled effective immunosuppression while limiting the incidence of drug-related adverse events. A fluorescence polarization immunoassay (FPIA) has been recently developed for the assessment of everolimus levels. The present study was d...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2006.08.013
更新日期：2006-12-01 00:00:00
abstract：OBJECTIVES:Hepcidin is the main regulator of systemic iron homeostasis and its expression is modulated by iron status, hypoxia, erythroid factors and inflammation. The aim of our study was to examine a relationship between level of hepcidin and iron status, erythropoietic activity, hypoxia and inflammation in exacerbat...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2015.07.010
更新日期：2015-12-01 00:00:00
abstract：OBJECTIVES:Eales' disease (ED) is an idiopathic retinal vasculitis condition, which affects retina of young adult males. The histopathological hallmark in ED is the adhesion of leukocytes to the endothelium and the infiltration of these cells into the retinal parenchyma. Phagocyte generated free radicals have been impl...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(03)00058-4
更新日期：2003-07-01 00:00:00
abstract：OBJECTIVES:To develop immunofluorometric procedures for measuring BRCA1 protein in various biological fluids and tissue extracts. DESIGN AND METHODS:Five commercially available monoclonal and polyclonal antibodies against BRCA1 were evaluated for developing competitive and non-competitive immunofluorometric procedures...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(97)00042-8
更新日期：1997-07-01 00:00:00
abstract：OBJECTIVES:Non-invasive prenatal aneuploidy testing (NIPT) by next-generation sequencing of circulating cell-free DNA in maternal plasma relies on chromosomal ratio (chrratio) measurements to detect aneuploid values that depart from euploid ratios. Diagnostic performances are known to depend on the fraction of fetal DN...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2018.06.015
更新日期：2018-09-01 00:00:00
abstract：OBJECTIVES:Reporting a case of inherited factor V deficiency and galactosemia. METHODS:A neonate was admitted with hematoma, jaundice, splenomegaly, diarrhea, anemia, abdominal ascites and bilateral cataracts that diagnosis of galactosaemia and factor V deficiency was established. RESULTS:Coinheritance of both coagul...
journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.clinbiochem.2011.12.024
更新日期：2012-03-01 00:00:00
abstract：OBJECTIVE:The detection of possible correlation between ribosomal protein RPS27A and multifunctional YB-1 expression in hepatocellular carcinoma (HCC). DESIGN AND METHODS:Tissue microarray slides containing totally 80 cores with 19 tissues of HCC, 1 tissue of hepatocholangiocarcinoma, 10 tissues of liver cirrhosis and...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2014.05.004
更新日期：2014-09-01 00:00:00
abstract：:Immunoturbidimetric assays for specific proteins are available on "open system" clinical chemistry analyzers. The analytical performance of nine immunoturbidimetric specific protein assays (C3, C4, CRP, Haptoglobin, IgA, IgG, IgM, RF, and Transferrin) was compared to immunonephelometry. Testing was performed on the Ab...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2009.06.016
更新日期：2009-10-01 00:00:00
abstract：:A simple and rapid method for serum sex hormone binding globulin (SHBG) was developed. Sera from pregnant women were pooled and the SHBG concentration was measured by Rosner's method. This pool was diluted with charcoal-treated and heated serum to give standards ranging in concentrations from 26.8-0.8 micrograms/L. Tw...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(85)80031-x
更新日期：1985-10-01 00:00:00
abstract：OBJECTIVE:We evaluated the erythrocyte lipid susceptibility to oxidation and erythrocyte antioxidant status in patients with myocardial infarction (MI). DESIGN AND METHODS:Patients with MI were divided into two group according to the severity of the disease as severe (n = 30) or milder (n = 25). Malondialdehyde as a m...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(98)00070-8
更新日期：1998-11-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to find a pre-interventional marker with the capacity to predict in-stent restenosis (ISR). Considering the anti-atherosclerotic role of adiponectin (APO), an adipocytokine with anti-inflammatory, anti-proliferative, anti-oxidative and anti-thrombotic properties, low plasma levels of...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2008.09.109
更新日期：2008-12-01 00:00:00
abstract：OBJECTIVES:Blood sampling/handling alters matrix metalloproteinases (MMP) and tissue inhibitors of metalloproteinases (TIMP) expression. The aim of this study is to evaluate the effects of high molecular weight heparin on MMP and TIMP expression in blood. DESIGN AND METHODS:We analyzed by gelatin zymography and ELISA ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2008.09.104
更新日期：2008-12-01 00:00:00
abstract：:An automated biotin-streptavidin procedure for measuring progesterone in serum is described. The method is linear up to 98.3 nmol/L and the calibration curve is stable for at least 14 days. The lower limit of progesterone detection is 0.70 nmol/L. Although serum is the preferred specimen, progesterone levels can be me...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(92)90105-2
更新日期：1992-12-01 00:00:00
abstract：OBJECTIVES:The aim of the study was to evaluate the concentration of interleukin 12 (IL-12), the activity of phospholipase A(2) (PLA(2)), and platelet-activating factor acetylhydrolase (PAF-AH) in type 1 diabetes (DM1) patients treated with intensive insulin therapy. DESIGN AND METHODS:Studied parameters were measured...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2009.07.023
更新日期：2009-11-01 00:00:00
abstract：OBJECTIVES:[-2]proPSA may discriminate prostate cancer from benign biopsy results. We characterized the pre-analytical stability of [-2]proPSA. DESIGN AND METHODS:22 volunteers, total PSA of 4.5-19.3microg/L, had blood drawn simultaneously. Baseline measurements were performed and samples were stored under various con...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2010.04.062
更新日期：2010-07-01 00:00:00
abstract：CONTEXT:Knee arthroplasty surgery is significant trauma, leading to an activated immune system causing inflammation and oxidative stress. Many current biomarkers are invasive, costly, and often slow to analyse, limiting their use for rapid inflammatory measurements. OBJECTIVES:We have examined the use of urinary neopt...
journal_title：Clinical biochemistry
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1016/j.clinbiochem.2018.11.002
更新日期：2019-01-01 00:00:00
abstract：OBJECTIVES:Analysis of serum/plasma methylmalonic acid (MMA) is important for the diagnosis and management of methylmalonic acidemia in pediatric populations. This work focuses on developing and validating a liquid chromatography tandem mass spectrometry (LC-MS/MS) method to monitor methylmalonic acidemia using a simpl...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2016.05.010
更新日期：2016-09-01 00:00:00
abstract：OBJECTIVE:To investigate the feasibility of detecting circulating cancer cells in peripheral blood from gastric cancer patients using Piwi-interacting RNAs (piRNAs) as markers. DESIGN AND METHODS:Real-time quantitative reverse transcription-polymerase chain reaction was used to analyze piR-651 and piR-823 levels in th...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2011.06.004
更新日期：2011-09-01 00:00:00
abstract：OBJECTIVES:Characterization of beta-galactosidase in leukocytes and fibroblasts of heterozygotes for GM1 type I. DESIGN AND METHODS:Leukocyte and fibroblast beta-galactosidase activity was determined fluorimetrically using 4-methylumbelliferyl-beta-D-galactoside as an artificial substrate. Optimum pH, Km, Vmax and the...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(00)00049-7
更新日期：2000-03-01 00:00:00
abstract：OBJECTIVES:To evaluate the effect of Matrix metalloproteinase 7 -181A/G polymorphism (rs11568818) on the risk of cancer, we performed a meta-analysis. DESIGN AND METHODS:24 case-control studies were identified by searching Pubmed, Embase, ISI, OVID MEDLINE and EBSCO databases. Odd ratios and corresponding 95% confiden...
journal_title：Clinical biochemistry
pub_type： 杂志文章,meta分析
doi：10.1016/j.clinbiochem.2013.07.015
更新日期：2013-11-01 00:00:00
abstract：OBJECTIVE:Plasma ammonia measurement is greatly influenced by pre-analytical conditions, which may lead to false positives. We wanted to evaluate the prevalence and clinical impact of plasma ammonia false positives in a pediatric population. DESIGN AND METHODS:Over a 28-month period, charts of patients with elevated a...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2007.01.024
更新日期：2007-05-01 00:00:00
abstract：OBJECTIVE:To determine Retinol Binding Protein 4 (RBP4) levels in patients with Familial Partial Lipodystrophy (FPLD). METHODS:Ten patients with FPLD and a control group (9 patients) were selected to participate in the study. RESULTS:RBP4-log levels were lower in patients with FPLD in comparison to control group (1.5...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2009.03.006
更新日期：2009-07-01 00:00:00
abstract：OBJECTIVES:The aim of the study was to examine the long-term effect of commonly used whole soy foods (soy flour) and purified daidzein (one major isoflavone and the precursor of equol) on renal function among prehypertensive postmenopausal women who are also equol producers, a population most likely to benefit from soy...
journal_title：Clinical biochemistry
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.clinbiochem.2014.05.054
更新日期：2014-09-01 00:00:00
