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An ELISA for plasma retinol-binding protein using monoclonal and polyclonal antibodies: plasma variation in normal and insulin resistant subjects.

Abstract：

OBJECTIVES:Plasma retinol-binding protein (RBP) has been linked to insulin resistance and cardiovascular risk, yet little is know of its natural variation in plasma. We examined this in normal subjects and compared plasma levels and variability in lean subjects and subjects with the metabolic syndrome. METHODS:We established an "in house" ELISA for plasma RBP and measured levels in 20 normal subjects over daylight hours and 2 subject groups, either lean or classified with the metabolic syndrome. RESULTS:Plasma RBP in normal subjects did not vary over the day with no differences between males and females. There was also no difference in plasma RBP levels and between the age- and sex-matched lean subjects compared to the metabolic syndrome group. CONCLUSION:The lack of variation in plasma RBP in normal subjects and the lack of difference between plasma RBP in normal and metabolic syndrome subjects suggest the link between plasma RBP and insulin resistance is tenuous. Investigating a large cohort over the diabetic non-diabetic spectrum may clarify this issue.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Lewis JG,Shand BI,Frampton CM,Elder PA

doi

10.1016/j.clinbiochem.2007.03.021

subject

Has Abstract

pub_date

2007-07-01 00:00:00

pages

828-34

issue

11

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(07)00162-2

journal_volume

40

pub_type

杂志文章

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