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Enzyme linked lectin assay (ELLA) for direct analysis of transferrin sialylation in serum samples.

Abstract：

OBJECTIVES:Glycosylation analysis provides many opportunities for diagnostics, but its complexity hampers its routine application. Aiming to alleviate this problem, we developed a simple assay that can measure sialylation of transferrin directly from serum. DESIGN AND METHODS:Transferrin samples with different levels of sialylation were prepared by desialylation. Enzyme-linked-lectin assay (ELLA) and high-performance anion-exchange chromatography (HPAEC) have been used to analyze transferrin sialylation. Periodate oxidation was used to oxidize carbohydrates on antibodies. RESULTS:ELLA was developed for the analysis of serum transferrin sialylation. Antibodies oxidized in situ with periodate have been used to capture transferrin from serum samples. Sialic acid on transferrin has been detected with Sambucus nigra agglutinin (SNA) lectin. Transferrin samples with different sialylation levels prepared by differential desialylation have been used as standards. Accuracy of the method has been confirmed by comparison to HPAEC analysis. CONCLUSIONS:A rapid and simple ELLA that can be routinely used for the analysis of serum transferrin sialylation has been developed.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Gornik O,Lauc G

doi

10.1016/j.clinbiochem.2007.01.010

subject

Has Abstract

pub_date

2007-06-01 00:00:00

pages

718-23

issue

9-10

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(07)00063-X

journal_volume

40

pub_type

杂志文章

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